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The map label for this gene is purR [H]

Identifier: 222527385

GI number: 222527385

Start: 5173130

End: 5174113

Strand: Direct

Name: purR [H]

Synonym: Chy400_4175

Alternate gene names: 222527385

Gene position: 5173130-5174113 (Clockwise)

Preceding gene: 222527384

Following gene: 222527386

Centisome position: 98.18

GC content: 53.15

Gene sequence:

>984_bases
ATGCCCACGATTAAAGATGTTGCACGTCGTGCCGGCGTTAGTGTAACGGCAGCCTCATATGCGCTTAACCGTACCGGGAC
GATTAGTGAAGAGACACGTCAACGGGTATTACAGGCAGCCGAAGAGTTAAACTACCATCCCAATGCCTTCGCCCGTCATC
TCAAAGCACGTCGTTCGCTTACCATCGGCGTGTTCATTACCCGCTTTGCCGGTGCGTTCTACGAAGAGATTCTCGATGGG
ATTCACGAGGTTATTCTTGCGACCGATTATGAGTTAATCGTCTGTCCAGAAACGCGAGTCGAACGTCGTATTTTCACGCA
TCGCCAGGTTGATGGCGCCATCGTCTTCGACACCAGCATTCCGAACGAACAGATTCTCCGGCTGGCAGGGCCGAAATTCC
CGATTGTCGTGATGGATCGGTACCTGGAAAATAAATTTGTCTTTCCCATCCTGCTCGATAACCGGCAAGGGGTTGTGCAG
GCGTTCAACCATCTCTACCGCCAGGGTTTACGGCGATTGGCCTATATCGCCGGTGCCCCAGACTCGTTCGACAACAACGA
GCGCATGCAAGCCTTTTTAAATGAGGCAACGCGCCACCACCTATCACTCCGGGTCTATCCCGGTAATTTTACCGAACAGT
CAGGATATGACGCTGCTCGTGCCATCATTGCTGATGGCGACTTACCTGAAGCGGTATTTTGCGCTAATGACCAGATGGCG
ATTGGCTTTATCCGGGCCATGCACGAACACGGCCTGGAAGCGCCTCGCGACATCGCTGTGGTTGGCTGCGACGACATTCA
GATTGCACGCTACGTTCAACCACCACTGTCAACCATTGGCGCCTCACGGCGTGAATGGGGTGGAACCGCAGCCAAATGGC
TCATGCGCTATATCGAACATGAAGAAACCTCACCACCGCAACGAATCCCGACAACCTTTATCGTCCGTGCCTCGTCATTG
AAGCGACCTGAATTGTTGGAGTAA

Upstream 100 bases:

>100_bases
CGAATTATCAAGACGGCTTAATCCACCGCATCACGATGGCTCATCCTGCTATTCTGGCCGGACTAGAGCGACTTGGGATC
GAACAGCTTCAGGAGGAGTA

Downstream 100 bases:

>100_bases
CTTGTGGACACGTACACTCTCCTCAACTACGAGCGTGAAGGTGCTATCAACTTTTTCCGAGCGGGTACCAATCTCGATCC
GAACAGTCCAGGCTATGGTC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPTIKDVARRAGVSVTAASYALNRTGTISEETRQRVLQAAEELNYHPNAFARHLKARRSLTIGVFITRFAGAFYEEILDG
IHEVILATDYELIVCPETRVERRIFTHRQVDGAIVFDTSIPNEQILRLAGPKFPIVVMDRYLENKFVFPILLDNRQGVVQ
AFNHLYRQGLRRLAYIAGAPDSFDNNERMQAFLNEATRHHLSLRVYPGNFTEQSGYDAARAIIADGDLPEAVFCANDQMA
IGFIRAMHEHGLEAPRDIAVVGCDDIQIARYVQPPLSTIGASRREWGGTAAKWLMRYIEHEETSPPQRIPTTFIVRASSL
KRPELLE

Sequences:

>Translated_327_residues
MPTIKDVARRAGVSVTAASYALNRTGTISEETRQRVLQAAEELNYHPNAFARHLKARRSLTIGVFITRFAGAFYEEILDG
IHEVILATDYELIVCPETRVERRIFTHRQVDGAIVFDTSIPNEQILRLAGPKFPIVVMDRYLENKFVFPILLDNRQGVVQ
AFNHLYRQGLRRLAYIAGAPDSFDNNERMQAFLNEATRHHLSLRVYPGNFTEQSGYDAARAIIADGDLPEAVFCANDQMA
IGFIRAMHEHGLEAPRDIAVVGCDDIQIARYVQPPLSTIGASRREWGGTAAKWLMRYIEHEETSPPQRIPTTFIVRASSL
KRPELLE
>Mature_326_residues
PTIKDVARRAGVSVTAASYALNRTGTISEETRQRVLQAAEELNYHPNAFARHLKARRSLTIGVFITRFAGAFYEEILDGI
HEVILATDYELIVCPETRVERRIFTHRQVDGAIVFDTSIPNEQILRLAGPKFPIVVMDRYLENKFVFPILLDNRQGVVQA
FNHLYRQGLRRLAYIAGAPDSFDNNERMQAFLNEATRHHLSLRVYPGNFTEQSGYDAARAIIADGDLPEAVFCANDQMAI
GFIRAMHEHGLEAPRDIAVVGCDDIQIARYVQPPLSTIGASRREWGGTAAKWLMRYIEHEETSPPQRIPTTFIVRASSLK
RPELLE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=326, Percent_Identity=33.4355828220859, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787948, Length=317, Percent_Identity=32.1766561514196, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=28.6144578313253, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=28.6144578313253, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=30.4794520547945, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=27.8787878787879, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788474, Length=296, Percent_Identity=28.0405405405405, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789456, Length=295, Percent_Identity=30.1694915254237, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790715, Length=309, Percent_Identity=25.2427184466019, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=27.5757575757576, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786268, Length=297, Percent_Identity=25.2525252525253, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=26.0188087774295, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=25.3776435045317, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36963; Mature: 36831

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTIKDVARRAGVSVTAASYALNRTGTISEETRQRVLQAAEELNYHPNAFARHLKARRSL
CCCHHHHHHHCCCEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
TIGVFITRFAGAFYEEILDGIHEVILATDYELIVCPETRVERRIFTHRQVDGAIVFDTSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCC
PNEQILRLAGPKFPIVVMDRYLENKFVFPILLDNRQGVVQAFNHLYRQGLRRLAYIAGAP
CHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
DSFDNNERMQAFLNEATRHHLSLRVYPGNFTEQSGYDAARAIIADGDLPEAVFCANDQMA
CCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHEEEECCCCCCEEEECCCCHH
IGFIRAMHEHGLEAPRDIAVVGCDDIQIARYVQPPLSTIGASRREWGGTAAKWLMRYIEH
HHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCHHHHCCCHHHCCCHHHHHHHHHHHH
EETSPPQRIPTTFIVRASSLKRPELLE
CCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
PTIKDVARRAGVSVTAASYALNRTGTISEETRQRVLQAAEELNYHPNAFARHLKARRSL
CCHHHHHHHCCCEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
TIGVFITRFAGAFYEEILDGIHEVILATDYELIVCPETRVERRIFTHRQVDGAIVFDTSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCC
PNEQILRLAGPKFPIVVMDRYLENKFVFPILLDNRQGVVQAFNHLYRQGLRRLAYIAGAP
CHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
DSFDNNERMQAFLNEATRHHLSLRVYPGNFTEQSGYDAARAIIADGDLPEAVFCANDQMA
CCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHEEEECCCCCCEEEECCCCHH
IGFIRAMHEHGLEAPRDIAVVGCDDIQIARYVQPPLSTIGASRREWGGTAAKWLMRYIEH
HHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCHHHHCCCHHHCCCHHHHHHHHHHHH
EETSPPQRIPTTFIVRASSLKRPELLE
CCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA