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The map label for this gene is yfiY [H]

Identifier: 222527354

GI number: 222527354

Start: 5129361

End: 5130449

Strand: Direct

Name: yfiY [H]

Synonym: Chy400_4143

Alternate gene names: 222527354

Gene position: 5129361-5130449 (Clockwise)

Preceding gene: 222527353

Following gene: 222527355

Centisome position: 97.35

GC content: 56.47

Gene sequence:

>1089_bases
ATGGTATACCGACTATTCATCCTTGTCATCCTGCTCACTCTGCTCGCCGCCTGTGGAGGTGCGCCGGCGACTGTCACGCC
TACGAGCGCACCAGATGCACTTACGGAAAATCCCGCACCGACAAGTGCGCCTGGCGCAACACCTGCTTCAACTGTTGCAA
CCTTCCCCGTCACCATCGAACACAAGTATGGAAGCACCACCATCCCGGCGGCGCCACAACGGGTAATTGCGCTAGGATAC
ACCGACCAGGACCCGATCCTCGCCCTGGGCGTGCGTCCCATTGCGGTACGCTACTGGTTTGGTGATGCCACACGCACAGC
CTGGCCGTGGCAAGAAGCTGCCTTCGGTGACGTTCGTCCGCAAGTTCTCAACATGCCCTTTGGTCAGCTCAACATTGAAA
CCATCGCCGCCCTAAATCCAGACCTGATCATCGCCGTCTCTGCCGGTATTACCGAAGAGGAGTACAACACCCTGAGTCAG
ATTGCCCCCACGCTCGCGCAATCTGATGCCTACGTCGATTTTGGTGTCCCCTGGCAGGAACAAACTCGTGTAATCGGGCA
GGCGCTCGGACGATCAGAACAGGCTGAGGCATTAATTTCCTCAATTGAAGCACGTTTTGCTGAACTCCGACAGCAGTATC
CACAGTTTGCCGGCGCCACAGCGGTCATTGCCTCACCAGCGAACGAAGGACAATTCTTCTTCTCTGGCACCCAACACGAA
CGGATGCGCTTTTTAACCTCGCTCGGCTTTGTACTCCCTGCCGAACTCGACCAGATAGCCAGCACATCGTTCTTCGGCTC
GATTAGCGGTGAACGCCTTGATCTTTTCGATACCGATGTCTTGATCTGGACGGTTACTCCTGAACAGCGAGCTGTTATTG
AAGCTAATCCGCTCTTTCAACAACTACGCGCCACGCAGGAAGGTCGCGTCATCTGGCTTGACTCTACCGGCGACGCCGAG
AGTGATCTGGTCGGGCCGGCCCTGGTCTATAGCAGTGTGTTGAGCTTGCCGCTCGTATTTGAAGAACTGGTACCCCAGCT
TGCCGCCGCTGTTGATGGCGACCCGACAACACAGGCTGCACAACGTTGA

Upstream 100 bases:

>100_bases
AGATCTCGTCAAGCACCTGGTTACAAGTCAATAACACGTGCCAACGACAAATCCTTGCGGAGCCGAACTATCTTGTCAAT
CCCTGAACGGAGTCTTCACT

Downstream 100 bases:

>100_bases
GGGGTATGTTGATAGCTAACCGTCCGGTTAAGCGGAGTCATCACCAAATGTTTGTGTCTACAGGAGAACCATCTATGACA
GCGTTTCAGCGCATCTCCAT

Product: periplasmic binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MVYRLFILVILLTLLAACGGAPATVTPTSAPDALTENPAPTSAPGATPASTVATFPVTIEHKYGSTTIPAAPQRVIALGY
TDQDPILALGVRPIAVRYWFGDATRTAWPWQEAAFGDVRPQVLNMPFGQLNIETIAALNPDLIIAVSAGITEEEYNTLSQ
IAPTLAQSDAYVDFGVPWQEQTRVIGQALGRSEQAEALISSIEARFAELRQQYPQFAGATAVIASPANEGQFFFSGTQHE
RMRFLTSLGFVLPAELDQIASTSFFGSISGERLDLFDTDVLIWTVTPEQRAVIEANPLFQQLRATQEGRVIWLDSTGDAE
SDLVGPALVYSSVLSLPLVFEELVPQLAAAVDGDPTTQAAQR

Sequences:

>Translated_362_residues
MVYRLFILVILLTLLAACGGAPATVTPTSAPDALTENPAPTSAPGATPASTVATFPVTIEHKYGSTTIPAAPQRVIALGY
TDQDPILALGVRPIAVRYWFGDATRTAWPWQEAAFGDVRPQVLNMPFGQLNIETIAALNPDLIIAVSAGITEEEYNTLSQ
IAPTLAQSDAYVDFGVPWQEQTRVIGQALGRSEQAEALISSIEARFAELRQQYPQFAGATAVIASPANEGQFFFSGTQHE
RMRFLTSLGFVLPAELDQIASTSFFGSISGERLDLFDTDVLIWTVTPEQRAVIEANPLFQQLRATQEGRVIWLDSTGDAE
SDLVGPALVYSSVLSLPLVFEELVPQLAAAVDGDPTTQAAQR
>Mature_362_residues
MVYRLFILVILLTLLAACGGAPATVTPTSAPDALTENPAPTSAPGATPASTVATFPVTIEHKYGSTTIPAAPQRVIALGY
TDQDPILALGVRPIAVRYWFGDATRTAWPWQEAAFGDVRPQVLNMPFGQLNIETIAALNPDLIIAVSAGITEEEYNTLSQ
IAPTLAQSDAYVDFGVPWQEQTRVIGQALGRSEQAEALISSIEARFAELRQQYPQFAGATAVIASPANEGQFFFSGTQHE
RMRFLTSLGFVLPAELDQIASTSFFGSISGERLDLFDTDVLIWTVTPEQRAVIEANPLFQQLRATQEGRVIWLDSTGDAE
SDLVGPALVYSSVLSLPLVFEELVPQLAAAVDGDPTTQAAQR

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=277, Percent_Identity=27.7978339350181, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 38923; Mature: 38923

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYRLFILVILLTLLAACGGAPATVTPTSAPDALTENPAPTSAPGATPASTVATFPVTIE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
HKYGSTTIPAAPQRVIALGYTDQDPILALGVRPIAVRYWFGDATRTAWPWQEAAFGDVRP
ECCCCCCCCCCCCEEEEEECCCCCCEEEECCCEEEEEEEECCCCCCCCCCHHCCCCCCCH
QVLNMPFGQLNIETIAALNPDLIIAVSAGITEEEYNTLSQIAPTLAQSDAYVDFGVPWQE
HHHCCCCCCCCCEEHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHH
QTRVIGQALGRSEQAEALISSIEARFAELRQQYPQFAGATAVIASPANEGQFFFSGTQHE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHH
RMRFLTSLGFVLPAELDQIASTSFFGSISGERLDLFDTDVLIWTVTPEQRAVIEANPLFQ
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCEEEECCHHHH
QLRATQEGRVIWLDSTGDAESDLVGPALVYSSVLSLPLVFEELVPQLAAAVDGDPTTQAA
HHHCCCCCCEEEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
QR
CC
>Mature Secondary Structure
MVYRLFILVILLTLLAACGGAPATVTPTSAPDALTENPAPTSAPGATPASTVATFPVTIE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
HKYGSTTIPAAPQRVIALGYTDQDPILALGVRPIAVRYWFGDATRTAWPWQEAAFGDVRP
ECCCCCCCCCCCCEEEEEECCCCCCEEEECCCEEEEEEEECCCCCCCCCCHHCCCCCCCH
QVLNMPFGQLNIETIAALNPDLIIAVSAGITEEEYNTLSQIAPTLAQSDAYVDFGVPWQE
HHHCCCCCCCCCEEHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHH
QTRVIGQALGRSEQAEALISSIEARFAELRQQYPQFAGATAVIASPANEGQFFFSGTQHE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHH
RMRFLTSLGFVLPAELDQIASTSFFGSISGERLDLFDTDVLIWTVTPEQRAVIEANPLFQ
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCEEEECCHHHH
QLRATQEGRVIWLDSTGDAESDLVGPALVYSSVLSLPLVFEELVPQLAAAVDGDPTTQAA
HHHCCCCCCEEEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
QR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]