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The map label for this gene is tpiA

Identifier: 222527344

GI number: 222527344

Start: 5117369

End: 5118121

Strand: Reverse

Name: tpiA

Synonym: Chy400_4132

Alternate gene names: 222527344

Gene position: 5118121-5117369 (Counterclockwise)

Preceding gene: 222527345

Following gene: 222527343

Centisome position: 97.14

GC content: 57.24

Gene sequence:

>753_bases
ATGCGTATTCCATTAATCGCCGGCAACTGGAAGATGTACAAGACGGTTGGCGAAGCAACGACGCTGGTGCGCGATTTGCT
GGCCGGTCTCGGTGAATTATCGGATCGCGAAGCTATCGTTTGTCCGCCCTTCACCGCACTCGCTGCCGTGGCAGCGCTGG
TCGCCGATAGCCCGCTGGGGTTGGGGGCGCAGAATCTGTACCCCGAAGCGCAGGGTGCGTTTACCGGTGAGGTCTCGCCG
CCAATGCTGGTTGATATTGGCTGTCGTTACGTCATTATTGGTCATAGCGAGCGTCGTCAGTATTTTGGCGAGAGTGATGC
GTTTGTGAATCGAAAATTGCGTGCCGCCCTTGCCCATGGTCTGCGTCCCATTGTGTGTGTTGGCGAGAGTAAGCCCCAGC
GTGACGCCGGGCAGGCCGAGCCGATTGTGACGGCACAGGTGCGTGCTGCCCTGCTTGAGGTGCCTCCGGATCAAATGGCA
AACGTGGTGATTGCTTACGAGCCGATCTGGGCCATTGGTACCGGCGATACGGCAACTCCTGCGGATGCGCAGGCGATGCA
TGCGGCAATTCGGGCAACTCTGGCCGAATTGTACGGTTCTGAGATTGCCGCAACGGTACGTATCCAGTACGGTGGCAGTG
TGAAGCCGGATAATATCGACGAATTGATGGCTCAGCCCGATATTGATGGTGCGTTGGTTGGTGGTGCTTCGTTACAGGCA
GCCAGTTTTCTGCGGATCATCCATTATCAGTAG

Upstream 100 bases:

>100_bases
AGCCGTTGGGGTTGGTTTGAGTTTTCTGCTTAGCGATGATCTGTTGTTTACATTAATACAAACCTTCGTTCGCCTGTTGT
AAATAGCTGCTGAGGTTTTC

Downstream 100 bases:

>100_bases
ATGGAACCTGAACGTAGTTCAGCGCAACAGACGTGCAGGCGACGGTTACTGAAGCCGTCGCCGTTTGCGCTTTCGGCTAC
CTCATACTCGTTGCGACAGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRIPLIAGNWKMYKTVGEATTLVRDLLAGLGELSDREAIVCPPFTALAAVAALVADSPLGLGAQNLYPEAQGAFTGEVSP
PMLVDIGCRYVIIGHSERRQYFGESDAFVNRKLRAALAHGLRPIVCVGESKPQRDAGQAEPIVTAQVRAALLEVPPDQMA
NVVIAYEPIWAIGTGDTATPADAQAMHAAIRATLAELYGSEIAATVRIQYGGSVKPDNIDELMAQPDIDGALVGGASLQA
ASFLRIIHYQ

Sequences:

>Translated_250_residues
MRIPLIAGNWKMYKTVGEATTLVRDLLAGLGELSDREAIVCPPFTALAAVAALVADSPLGLGAQNLYPEAQGAFTGEVSP
PMLVDIGCRYVIIGHSERRQYFGESDAFVNRKLRAALAHGLRPIVCVGESKPQRDAGQAEPIVTAQVRAALLEVPPDQMA
NVVIAYEPIWAIGTGDTATPADAQAMHAAIRATLAELYGSEIAATVRIQYGGSVKPDNIDELMAQPDIDGALVGGASLQA
ASFLRIIHYQ
>Mature_250_residues
MRIPLIAGNWKMYKTVGEATTLVRDLLAGLGELSDREAIVCPPFTALAAVAALVADSPLGLGAQNLYPEAQGAFTGEVSP
PMLVDIGCRYVIIGHSERRQYFGESDAFVNRKLRAALAHGLRPIVCVGESKPQRDAGQAEPIVTAQVRAALLEVPPDQMA
NVVIAYEPIWAIGTGDTATPADAQAMHAAIRATLAELYGSEIAATVRIQYGGSVKPDNIDELMAQPDIDGALVGGASLQA
ASFLRIIHYQ

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=42.914979757085, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=42.914979757085, Blast_Score=187, Evalue=5e-48,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=45.3441295546559, Blast_Score=206, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=42.914979757085, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=43.1451612903226, Blast_Score=192, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=45.748987854251, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=45.748987854251, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=45.748987854251, Blast_Score=204, Evalue=3e-53,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CHLAA (P96744)

Other databases:

- EMBL:   CP000909
- EMBL:   U73963
- RefSeq:   YP_001637392.1
- ProteinModelPortal:   P96744
- SMR:   P96744
- GeneID:   5825634
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3825
- HOGENOM:   HBG708281
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26475; Mature: 26475

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPLIAGNWKMYKTVGEATTLVRDLLAGLGELSDREAIVCPPFTALAAVAALVADSPLG
CCCCEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCC
LGAQNLYPEAQGAFTGEVSPPMLVDIGCRYVIIGHSERRQYFGESDAFVNRKLRAALAHG
CCHHHCCCCCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHC
LRPIVCVGESKPQRDAGQAEPIVTAQVRAALLEVPPDQMANVVIAYEPIWAIGTGDTATP
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCEEEEECCEEEEECCCCCCC
ADAQAMHAAIRATLAELYGSEIAATVRIQYGGSVKPDNIDELMAQPDIDGALVGGASLQA
CHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHCCCCCCCEEECCCHHHH
ASFLRIIHYQ
HHHHHHHCCC
>Mature Secondary Structure
MRIPLIAGNWKMYKTVGEATTLVRDLLAGLGELSDREAIVCPPFTALAAVAALVADSPLG
CCCCEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCC
LGAQNLYPEAQGAFTGEVSPPMLVDIGCRYVIIGHSERRQYFGESDAFVNRKLRAALAHG
CCHHHCCCCCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHC
LRPIVCVGESKPQRDAGQAEPIVTAQVRAALLEVPPDQMANVVIAYEPIWAIGTGDTATP
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCEEEEECCEEEEECCCCCCC
ADAQAMHAAIRATLAELYGSEIAATVRIQYGGSVKPDNIDELMAQPDIDGALVGGASLQA
CHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHCCCCCCCEEECCCHHHH
ASFLRIIHYQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9037042