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The map label for this gene is metG

Identifier: 222526954

GI number: 222526954

Start: 4651962

End: 4653764

Strand: Direct

Name: metG

Synonym: Chy400_3732

Alternate gene names: 222526954

Gene position: 4651962-4653764 (Clockwise)

Preceding gene: 222526953

Following gene: 222526956

Centisome position: 88.29

GC content: 56.3

Gene sequence:

>1803_bases
ATGCCTGAACATATCCTGGTTGCCGTTGCCTGGCCGTATGCCAATGGGCCGCGTCACATCGGCCACGTTGCCGGATTTGG
TGTCCCGGCTGATATTTTTGCCCGTTACCACCGTTTGCGGGGGAACCACGTGTTGATGATTTCGGGCACTGATGAGCACG
GTACGCCGATTACGCTGGTTGCCGACAAAGAAGGAACCACGCCCCAGGCGATTGCTGATCGCTACAACAAAATCATCGGC
GACGATCTCTACAATCTTGGGTTGAGCTACGATATTTTCACGCGCACGACAACCGCAAACCACTATGCGGTAACCCAGGA
CATCTTCCGCACGCTCTACGAGCGGGGCTATATTATTCGCCAGGAGACACTGGGTGCGTTCTCGGCCACGACTGGCCGTA
CTCTGCCTGACCGCTACATCGAAGGCACCTGTCCAATCTGCGGCTACGATGAAGCACGTGGCGATCAGTGTGATAACTGT
GGCAGCCAGCTCGATCCAACCGACCTCATCAATCCGCGCTCGAAGGTGGATGGACAGCCACCGGTGTTCAAACCTACCGA
ACACTTCTTTCTCGATCTGCCGGCATTTGCCGAACAGTTGCACGCCTGGATTGATCGGCAAACCCACTGGCGCCCAAACG
TGCGTAACTTCTCGCTCAACTTCTTGAAAGAACTGAAGCCACGGGCTATCACGCGCGATCTGGAGTGGGGTGTACCCATT
CCCCTGCCGGAATATGTCCATCGTGACGATAAAAAGATTTATGTCTGGTTTGACGCGGTGATCGGCTACCTGTCGGCCAG
CATCGAATGGGCACGCAATAGTGGTCAACCTGATGCATGGCGTACCTGGTGGCAAAATCCGGCTGCCCGCCATTTCTACT
TCATGGGCAAAGATAACATCGTTTTCCACACGGTTATCTGGCCGGCGATGCTGCTCGGTTATGGTGCAGGTGGTGATTTT
GGTACCGATCCGAGTGGAACCTACCAGGGCGTTCCCCTCCAATTGCCGTACAACGTTGTATCGAGTGAATTTCTCACGAT
GGAGGGGAAGAAGTTCTCAAGCTCGCGTGGGATTGTTATCTACGTCAACGATTTTCTCAGCCGGTACGATGCCGATGCGC
TGCGCTACTTTCTGACCATCGCCGGGCCTGAAAACCAGGATACCGATTTCACCTGGGCCGAGTTTGTGCGCCGGAACAAT
GACGAATTAGTGGCAACGTGGGGCAATCTGGTCAACCGCACGCTGACCAATGTCTACAAGAATTTTGGGAGCGTCCCCCA
ACCCGGCCCCCTGACCGCAACCGACGAGCAGTTGATACAAGAGGTGAACGGCGGTCTGGAGACCGTGGGCGATCTGCTGG
CTACAGCTCGCTTCAAAGCCGCGCTGAGCGAGGCGATGCGGCTGGCCGCGCAGGTGAATATCTACCTGAGCGAACAGGAG
CCGTGGAAAGTGATCAAGAGTGATCGCGAGCGTGCTGCAACCATCTGGTACGTCGCGCTGCGCTGTGTTGACACACTCAA
GATCATCTTCACACCGTTCCTGCCCTTCTCCAGCCAGCGCCTGCACGAGTATTTGGGGTATGACGGTTACATCGCCGGCC
CGCTCTCTTTCCGCGATGTCACCGAAGCCGATGGCCGCGTTCATCGCGTACTGACCGGCAATTATGCCGAATGGGTTGGG
CGCTGGCAACCATCAGCGTTACCGGTGGGGCAGGCGCTCCGCCAACCGCAGCCACTCTTCAAGAAACTTGATGAAAAGGT
TGTTGATGAAGAACTGGCGCGGATGCAGAGTCGGGCGTCATAG

Upstream 100 bases:

>100_bases
GCCTTTCCCCTCTACCACAGATATACTCACAGTGTTCTGCCGCAAGATTCATTGGAAGCACGTTCGTCAGTTGTTATTGA
TTGACGTTTGAGGTGATACA

Downstream 100 bases:

>100_bases
GCGGCGTGGTCGCCATTACAACGCACGTCGCCATTGGAGAGAGCATTAACCCCTGGATCGATAACTATCGTTCGCTACAC
CATAAAACAGGAGCACTGGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MPEHILVAVAWPYANGPRHIGHVAGFGVPADIFARYHRLRGNHVLMISGTDEHGTPITLVADKEGTTPQAIADRYNKIIG
DDLYNLGLSYDIFTRTTTANHYAVTQDIFRTLYERGYIIRQETLGAFSATTGRTLPDRYIEGTCPICGYDEARGDQCDNC
GSQLDPTDLINPRSKVDGQPPVFKPTEHFFLDLPAFAEQLHAWIDRQTHWRPNVRNFSLNFLKELKPRAITRDLEWGVPI
PLPEYVHRDDKKIYVWFDAVIGYLSASIEWARNSGQPDAWRTWWQNPAARHFYFMGKDNIVFHTVIWPAMLLGYGAGGDF
GTDPSGTYQGVPLQLPYNVVSSEFLTMEGKKFSSSRGIVIYVNDFLSRYDADALRYFLTIAGPENQDTDFTWAEFVRRNN
DELVATWGNLVNRTLTNVYKNFGSVPQPGPLTATDEQLIQEVNGGLETVGDLLATARFKAALSEAMRLAAQVNIYLSEQE
PWKVIKSDRERAATIWYVALRCVDTLKIIFTPFLPFSSQRLHEYLGYDGYIAGPLSFRDVTEADGRVHRVLTGNYAEWVG
RWQPSALPVGQALRQPQPLFKKLDEKVVDEELARMQSRAS

Sequences:

>Translated_600_residues
MPEHILVAVAWPYANGPRHIGHVAGFGVPADIFARYHRLRGNHVLMISGTDEHGTPITLVADKEGTTPQAIADRYNKIIG
DDLYNLGLSYDIFTRTTTANHYAVTQDIFRTLYERGYIIRQETLGAFSATTGRTLPDRYIEGTCPICGYDEARGDQCDNC
GSQLDPTDLINPRSKVDGQPPVFKPTEHFFLDLPAFAEQLHAWIDRQTHWRPNVRNFSLNFLKELKPRAITRDLEWGVPI
PLPEYVHRDDKKIYVWFDAVIGYLSASIEWARNSGQPDAWRTWWQNPAARHFYFMGKDNIVFHTVIWPAMLLGYGAGGDF
GTDPSGTYQGVPLQLPYNVVSSEFLTMEGKKFSSSRGIVIYVNDFLSRYDADALRYFLTIAGPENQDTDFTWAEFVRRNN
DELVATWGNLVNRTLTNVYKNFGSVPQPGPLTATDEQLIQEVNGGLETVGDLLATARFKAALSEAMRLAAQVNIYLSEQE
PWKVIKSDRERAATIWYVALRCVDTLKIIFTPFLPFSSQRLHEYLGYDGYIAGPLSFRDVTEADGRVHRVLTGNYAEWVG
RWQPSALPVGQALRQPQPLFKKLDEKVVDEELARMQSRAS
>Mature_599_residues
PEHILVAVAWPYANGPRHIGHVAGFGVPADIFARYHRLRGNHVLMISGTDEHGTPITLVADKEGTTPQAIADRYNKIIGD
DLYNLGLSYDIFTRTTTANHYAVTQDIFRTLYERGYIIRQETLGAFSATTGRTLPDRYIEGTCPICGYDEARGDQCDNCG
SQLDPTDLINPRSKVDGQPPVFKPTEHFFLDLPAFAEQLHAWIDRQTHWRPNVRNFSLNFLKELKPRAITRDLEWGVPIP
LPEYVHRDDKKIYVWFDAVIGYLSASIEWARNSGQPDAWRTWWQNPAARHFYFMGKDNIVFHTVIWPAMLLGYGAGGDFG
TDPSGTYQGVPLQLPYNVVSSEFLTMEGKKFSSSRGIVIYVNDFLSRYDADALRYFLTIAGPENQDTDFTWAEFVRRNND
ELVATWGNLVNRTLTNVYKNFGSVPQPGPLTATDEQLIQEVNGGLETVGDLLATARFKAALSEAMRLAAQVNIYLSEQEP
WKVIKSDRERAATIWYVALRCVDTLKIIFTPFLPFSSQRLHEYLGYDGYIAGPLSFRDVTEADGRVHRVLTGNYAEWVGR
WQPSALPVGQALRQPQPLFKKLDEKVVDEELARMQSRAS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=597, Percent_Identity=35.3433835845896, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI24308436, Length=542, Percent_Identity=28.2287822878229, Blast_Score=169, Evalue=9e-42,
Organism=Escherichia coli, GI1788432, Length=532, Percent_Identity=32.3308270676692, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1786861, Length=223, Percent_Identity=23.7668161434978, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=605, Percent_Identity=32.2314049586777, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI212645096, Length=527, Percent_Identity=25.2371916508539, Blast_Score=156, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71992781, Length=363, Percent_Identity=24.5179063360882, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=596, Percent_Identity=34.8993288590604, Blast_Score=352, Evalue=9e-98,
Organism=Saccharomyces cerevisiae, GI6321610, Length=560, Percent_Identity=23.2142857142857, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24655311, Length=600, Percent_Identity=34.5, Blast_Score=347, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21358483, Length=535, Percent_Identity=24.2990654205607, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CHLAA (A9WKM1)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001637038.1
- GeneID:   5825267
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3464
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   CLSK2477367
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 67972; Mature: 67841

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEHILVAVAWPYANGPRHIGHVAGFGVPADIFARYHRLRGNHVLMISGTDEHGTPITLV
CCCEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
ADKEGTTPQAIADRYNKIIGDDLYNLGLSYDIFTRTTTANHYAVTQDIFRTLYERGYIIR
ECCCCCCHHHHHHHHHHHHCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCEEEE
QETLGAFSATTGRTLPDRYIEGTCPICGYDEARGDQCDNCGSQLDPTDLINPRSKVDGQP
EHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHCCCCC
PVFKPTEHFFLDLPAFAEQLHAWIDRQTHWRPNVRNFSLNFLKELKPRAITRDLEWGVPI
CCCCCCHHHEEECHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCCCCC
PLPEYVHRDDKKIYVWFDAVIGYLSASIEWARNSGQPDAWRTWWQNPAARHFYFMGKDNI
CCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCE
VFHTVIWPAMLLGYGAGGDFGTDPSGTYQGVPLQLPYNVVSSEFLTMEGKKFSSSRGIVI
EEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHCCHHEEECCCCCCCCCCEEE
YVNDFLSRYDADALRYFLTIAGPENQDTDFTWAEFVRRNNDELVATWGNLVNRTLTNVYK
EHHHHHHHHCHHHEEEEEEEECCCCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
NFGSVPQPGPLTATDEQLIQEVNGGLETVGDLLATARFKAALSEAMRLAAQVNIYLSEQE
HHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
PWKVIKSDRERAATIWYVALRCVDTLKIIFTPFLPFSSQRLHEYLGYDGYIAGPLSFRDV
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCCCC
TEADGRVHRVLTGNYAEWVGRWQPSALPVGQALRQPQPLFKKLDEKVVDEELARMQSRAS
CCCCCCEEEEEECCHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEHILVAVAWPYANGPRHIGHVAGFGVPADIFARYHRLRGNHVLMISGTDEHGTPITLV
CCEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
ADKEGTTPQAIADRYNKIIGDDLYNLGLSYDIFTRTTTANHYAVTQDIFRTLYERGYIIR
ECCCCCCHHHHHHHHHHHHCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCEEEE
QETLGAFSATTGRTLPDRYIEGTCPICGYDEARGDQCDNCGSQLDPTDLINPRSKVDGQP
EHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHCCCCC
PVFKPTEHFFLDLPAFAEQLHAWIDRQTHWRPNVRNFSLNFLKELKPRAITRDLEWGVPI
CCCCCCHHHEEECHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCCCCC
PLPEYVHRDDKKIYVWFDAVIGYLSASIEWARNSGQPDAWRTWWQNPAARHFYFMGKDNI
CCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCE
VFHTVIWPAMLLGYGAGGDFGTDPSGTYQGVPLQLPYNVVSSEFLTMEGKKFSSSRGIVI
EEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHCCHHEEECCCCCCCCCCEEE
YVNDFLSRYDADALRYFLTIAGPENQDTDFTWAEFVRRNNDELVATWGNLVNRTLTNVYK
EHHHHHHHHCHHHEEEEEEEECCCCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
NFGSVPQPGPLTATDEQLIQEVNGGLETVGDLLATARFKAALSEAMRLAAQVNIYLSEQE
HHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
PWKVIKSDRERAATIWYVALRCVDTLKIIFTPFLPFSSQRLHEYLGYDGYIAGPLSFRDV
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCCCC
TEADGRVHRVLTGNYAEWVGRWQPSALPVGQALRQPQPLFKKLDEKVVDEELARMQSRAS
CCCCCCEEEEEECCHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA