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The map label for this gene is neo [H]

Identifier: 222525953

GI number: 222525953

Start: 3343523

End: 3344308

Strand: Direct

Name: neo [H]

Synonym: Chy400_2709

Alternate gene names: 222525953

Gene position: 3343523-3344308 (Clockwise)

Preceding gene: 222525952

Following gene: 222525958

Centisome position: 63.46

GC content: 56.36

Gene sequence:

>786_bases
ATGGTGCGCAGTCACGAACTTCCCGTAGCTGTTCAGCGGCTCTTGCAGGGTAGCAGTGTTGAACTGATGACAGATGGTTG
TACAGCGGCGCAGGTCTATCGTATCGAGCAGGATGGTCGTTGTATGTACCTGAAGATGCAGTCGCTTGCCGATACGCCGT
CGCTGCACGTGGAAGCCCGTATCCTCGATTGGTTGCAGGGTAAACTGCCGGTGCCGGAGGTGCTGGTGTATGTGGCCGAT
GACCGGTATGAATATCTGGTTTCGACCGAGGTGGCCGGTCAGAATGGGGTCAGGGCGATGGCGACGCTGCGAGCGGAAGC
GCTGGTTGAGCTGATGGCGAGTGGCCTGCGCATGATCCACGCGCTCGATCCTGCTACCTGCCCGTTCGATGCCGGTCTTG
AGTTGCGGTTGATGCAGGCGCGTGCCAATGTGTTGAACGGTCTGGTTGATGAGACCGATTTTGATCCGGAGCGGCTCGGT
AGCACGACCGCTCAGGATATTCTGGCCGCTCTGGAACGCGAGCGCCCGGCGACAGATGATCTGGTGTTCACCCATGGCGA
TTACTGTCTGCCTAATATCATTGTGCGCGATGGTATGATCGGTGGCTTTATTGACTGGGGGCGGGCAGGAGTTGCCGACC
GTTACAACGATCTGGCGATTGCCTCACGGAGTATTCGTTATAATCTAGGTCCCCAGTACGAGCAGTACTTTTTTGCGTGT
TACGGCCTTGAACAGGTTGATGCCGGTAAAATCGCGTATTACCGCATGCTGGACGAATTATTCTAG

Upstream 100 bases:

>100_bases
GCTACCAGAGGGTCGGGATAGGCAGGGAGTGACGAGTCGCCCGGATGCAGGATCGGCCAATGTCTGTTTCAGATGGTCTG
GTAGGAATGAATGAGGTCGT

Downstream 100 bases:

>100_bases
CGTGGCTCTGCGGATCGTACCGCTGACCGCACCAAAGCCGATGCGATAGCCGTCACCCTGTGCCCAGCCGCGCATTGTGA
CGGTATCACCATCTTCGAGC

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: APH(3')-II; APH(3')II; Kanamycin kinase, type II; Neomycin-kanamycin phosphotransferase type II [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MVRSHELPVAVQRLLQGSSVELMTDGCTAAQVYRIEQDGRCMYLKMQSLADTPSLHVEARILDWLQGKLPVPEVLVYVAD
DRYEYLVSTEVAGQNGVRAMATLRAEALVELMASGLRMIHALDPATCPFDAGLELRLMQARANVLNGLVDETDFDPERLG
STTAQDILAALERERPATDDLVFTHGDYCLPNIIVRDGMIGGFIDWGRAGVADRYNDLAIASRSIRYNLGPQYEQYFFAC
YGLEQVDAGKIAYYRMLDELF

Sequences:

>Translated_261_residues
MVRSHELPVAVQRLLQGSSVELMTDGCTAAQVYRIEQDGRCMYLKMQSLADTPSLHVEARILDWLQGKLPVPEVLVYVAD
DRYEYLVSTEVAGQNGVRAMATLRAEALVELMASGLRMIHALDPATCPFDAGLELRLMQARANVLNGLVDETDFDPERLG
STTAQDILAALERERPATDDLVFTHGDYCLPNIIVRDGMIGGFIDWGRAGVADRYNDLAIASRSIRYNLGPQYEQYFFAC
YGLEQVDAGKIAYYRMLDELF
>Mature_261_residues
MVRSHELPVAVQRLLQGSSVELMTDGCTAAQVYRIEQDGRCMYLKMQSLADTPSLHVEARILDWLQGKLPVPEVLVYVAD
DRYEYLVSTEVAGQNGVRAMATLRAEALVELMASGLRMIHALDPATCPFDAGLELRLMQARANVLNGLVDETDFDPERLG
STTAQDILAALERERPATDDLVFTHGDYCLPNIIVRDGMIGGFIDWGRAGVADRYNDLAIASRSIRYNLGPQYEQYFFAC
YGLEQVDAGKIAYYRMLDELF

Specific function: Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 29138; Mature: 29138

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00109 PROTEIN_KINASE_TYR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRSHELPVAVQRLLQGSSVELMTDGCTAAQVYRIEQDGRCMYLKMQSLADTPSLHVEAR
CCCCCCCCHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEEEEECHHCCCCCCEEHHH
ILDWLQGKLPVPEVLVYVADDRYEYLVSTEVAGQNGVRAMATLRAEALVELMASGLRMIH
HHHHHCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALDPATCPFDAGLELRLMQARANVLNGLVDETDFDPERLGSTTAQDILAALERERPATDD
HCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCC
LVFTHGDYCLPNIIVRDGMIGGFIDWGRAGVADRYNDLAIASRSIRYNLGPQYEQYFFAC
EEEECCCCCCCHHHEECCCCCCCHHCCCCCCCCCCCHHHHHHCCEEECCCCCHHHHHHHH
YGLEQVDAGKIAYYRMLDELF
CCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MVRSHELPVAVQRLLQGSSVELMTDGCTAAQVYRIEQDGRCMYLKMQSLADTPSLHVEAR
CCCCCCCCHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEEEEECHHCCCCCCEEHHH
ILDWLQGKLPVPEVLVYVADDRYEYLVSTEVAGQNGVRAMATLRAEALVELMASGLRMIH
HHHHHCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALDPATCPFDAGLELRLMQARANVLNGLVDETDFDPERLGSTTAQDILAALERERPATDD
HCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCC
LVFTHGDYCLPNIIVRDGMIGGFIDWGRAGVADRYNDLAIASRSIRYNLGPQYEQYFFAC
EEEECCCCCCCHHHEECCCCCCCHHCCCCCCCCCCCHHHHHHCCEEECCCCCHHHHHHHH
YGLEQVDAGKIAYYRMLDELF
CCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6295884; 3889831; 12628253 [H]