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The map label for this gene is pdhB [H]

Identifier: 222525383

GI number: 222525383

Start: 2703119

End: 2704114

Strand: Direct

Name: pdhB [H]

Synonym: Chy400_2128

Alternate gene names: 222525383

Gene position: 2703119-2704114 (Clockwise)

Preceding gene: 222525382

Following gene: 222525384

Centisome position: 51.3

GC content: 56.22

Gene sequence:

>996_bases
ATGCCTGTGATTACCTATCGCCAGGCTCTGAACGATACGCTTGGTGAGGAATTAGCTCGCGATCCGAACGTCTTTCTGAT
GGGCGAAGAGATCGGCGTCTTTCAGGGTTCGTATCGGGTTACCGAGGGTTTATTGGCCGAGTTTGGCCCGAAGCGGGTAG
TTGATACCCCCATCGCTGAAGAAGGTTTTGTCGGCGTGGCGATTGGCGCCGCGATGCTGGGGCTGCGTCCCGTCGTCGAG
ATTATGACCATTAACTTTATTCTGGTCGCTATCGACCAGGTCGTTAACCACGCCTCAAAGATTCACTATATGTTTGGTGG
TCAGGTGAGTGTGCCGCTCGTGATTCGCACTCCTTCCGGCGGTACCGGTCAGTTGGCTGCGACGCACTCACAGTCCTTCG
AGAACTGGTTTGCCTACTGCCCTGGTCTGAAAGTTGTGGCACCGGCAACTCCCTACGATGCTAAAGGCTTGCTGCGTGCC
GCCATTCGTGATGATGACCCGGTCATCTTTATCGAGTCGCTGGCCCTCTACGACACGAAGGGCGAAGTCCCCGAAGACGA
TGACTATGTCGTGCCGATTGGGGTTGCGGAAGTCAAGCGTCAGGGGACTGATGTCACCGTGGTTTCCTATTCGCGTATGA
CGGCGGTAGCTCTCCAGGTGGCCCAGCGCATGGAACAAGAGGGGATTAGCGTCGAGGTCGTCGATTTGCGCTCGCTCCGG
CCACTCGACCGCCCCACAATCATTGAGTCGGTGAAGAAGACCAATCGTGCGGTCGTTATCGCTGAAGACTGGTATTCATA
CGGGGTGACGGCTGAAATCGCGGCGACGATCCAGGAAGAGGCGTTCGATTATCTCGATGCGCCAGTCTATCGGGTGGCCG
GTCTCGAAGTGCCGCTGCCATACGCCAAGGAATTATCGGCTGCTTCAAAGCCGAATGCTAACAGCCTGATTTACGCTATT
CGACAGGTAATGCGCGGCACCAGGCGCATGCGATAA

Upstream 100 bases:

>100_bases
ATGTGTAACGATGTGAGAGTACGTAACGCATGTATCTGATCAATAAGTTCCGCGATAGCCGGCCATACATCCGGCAATTG
TTGAATGCAAAGAGGTTTCT

Downstream 100 bases:

>100_bases
CTGTTCTGTAATGAATTACAGGAGGCTCCATGGGTGAAGTAACCATGCCTCGTCTCTCCGACACAATGTCGGAAGGTACT
GTGGGCCGCTGGTTGAAGAA

Product: transketolase central subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MPVITYRQALNDTLGEELARDPNVFLMGEEIGVFQGSYRVTEGLLAEFGPKRVVDTPIAEEGFVGVAIGAAMLGLRPVVE
IMTINFILVAIDQVVNHASKIHYMFGGQVSVPLVIRTPSGGTGQLAATHSQSFENWFAYCPGLKVVAPATPYDAKGLLRA
AIRDDDPVIFIESLALYDTKGEVPEDDDYVVPIGVAEVKRQGTDVTVVSYSRMTAVALQVAQRMEQEGISVEVVDLRSLR
PLDRPTIIESVKKTNRAVVIAEDWYSYGVTAEIAATIQEEAFDYLDAPVYRVAGLEVPLPYAKELSAASKPNANSLIYAI
RQVMRGTRRMR

Sequences:

>Translated_331_residues
MPVITYRQALNDTLGEELARDPNVFLMGEEIGVFQGSYRVTEGLLAEFGPKRVVDTPIAEEGFVGVAIGAAMLGLRPVVE
IMTINFILVAIDQVVNHASKIHYMFGGQVSVPLVIRTPSGGTGQLAATHSQSFENWFAYCPGLKVVAPATPYDAKGLLRA
AIRDDDPVIFIESLALYDTKGEVPEDDDYVVPIGVAEVKRQGTDVTVVSYSRMTAVALQVAQRMEQEGISVEVVDLRSLR
PLDRPTIIESVKKTNRAVVIAEDWYSYGVTAEIAATIQEEAFDYLDAPVYRVAGLEVPLPYAKELSAASKPNANSLIYAI
RQVMRGTRRMR
>Mature_330_residues
PVITYRQALNDTLGEELARDPNVFLMGEEIGVFQGSYRVTEGLLAEFGPKRVVDTPIAEEGFVGVAIGAAMLGLRPVVEI
MTINFILVAIDQVVNHASKIHYMFGGQVSVPLVIRTPSGGTGQLAATHSQSFENWFAYCPGLKVVAPATPYDAKGLLRAA
IRDDDPVIFIESLALYDTKGEVPEDDDYVVPIGVAEVKRQGTDVTVVSYSRMTAVALQVAQRMEQEGISVEVVDLRSLRP
LDRPTIIESVKKTNRAVVIAEDWYSYGVTAEIAATIQEEAFDYLDAPVYRVAGLEVPLPYAKELSAASKPNANSLIYAIR
QVMRGTRRMR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=47.8527607361963, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=45.7055214723926, Blast_Score=307, Evalue=9e-84,
Organism=Homo sapiens, GI4557353, Length=299, Percent_Identity=40.8026755852843, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI34101272, Length=299, Percent_Identity=40.8026755852843, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=49.3865030674847, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17506935, Length=299, Percent_Identity=40.133779264214, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=47.239263803681, Blast_Score=318, Evalue=8e-88,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=49.2354740061162, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=49.2354740061162, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI160714832, Length=299, Percent_Identity=38.7959866220736, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI160714828, Length=299, Percent_Identity=38.7959866220736, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=56.6666666666667, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=56.6666666666667, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36224; Mature: 36093

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITYRQALNDTLGEELARDPNVFLMGEEIGVFQGSYRVTEGLLAEFGPKRVVDTPIAE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCHHHHHHHHHHCCCCCCCCCCCCC
EGFVGVAIGAAMLGLRPVVEIMTINFILVAIDQVVNHASKIHYMFGGQVSVPLVIRTPSG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEEECCCC
GTGQLAATHSQSFENWFAYCPGLKVVAPATPYDAKGLLRAAIRDDDPVIFIESLALYDTK
CCCCEEECCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCC
GEVPEDDDYVVPIGVAEVKRQGTDVTVVSYSRMTAVALQVAQRMEQEGISVEVVDLRSLR
CCCCCCCCEEEEECHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PLDRPTIIESVKKTNRAVVIAEDWYSYGVTAEIAATIQEEAFDYLDAPVYRVAGLEVPLP
CCCCHHHHHHHHHCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCCCC
YAKELSAASKPNANSLIYAIRQVMRGTRRMR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVITYRQALNDTLGEELARDPNVFLMGEEIGVFQGSYRVTEGLLAEFGPKRVVDTPIAE
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCHHHHHHHHHHCCCCCCCCCCCCC
EGFVGVAIGAAMLGLRPVVEIMTINFILVAIDQVVNHASKIHYMFGGQVSVPLVIRTPSG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEEECCCC
GTGQLAATHSQSFENWFAYCPGLKVVAPATPYDAKGLLRAAIRDDDPVIFIESLALYDTK
CCCCEEECCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCC
GEVPEDDDYVVPIGVAEVKRQGTDVTVVSYSRMTAVALQVAQRMEQEGISVEVVDLRSLR
CCCCCCCCEEEEECHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PLDRPTIIESVKKTNRAVVIAEDWYSYGVTAEIAATIQEEAFDYLDAPVYRVAGLEVPLP
CCCCHHHHHHHHHCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCCCC
YAKELSAASKPNANSLIYAIRQVMRGTRRMR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]