Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 222523694

GI number: 222523694

Start: 472847

End: 473497

Strand: Reverse

Name: thiE

Synonym: Chy400_0400

Alternate gene names: 222523694

Gene position: 473497-472847 (Counterclockwise)

Preceding gene: 222523695

Following gene: 222523693

Centisome position: 8.99

GC content: 56.37

Gene sequence:

>651_bases
GTGAGTATCGCCCATATTGTTGACTGGCGGCTGTATGTCGTCACTGATGCCGGGCTGTCGCGAGGCCGTTCACATCGCGC
AGTGATTGAAGCGGCGATAGTTGGTGGCGCCACAGTTGTGCAGTATCGGGAGAAGCATGCCTCAACCCGGCAGATGATTG
AAGAAGCGCTGGAATTGCGTGATCTGACCCGACGGGCTGGGGTGCCACTGATCGTCAATGATCGGGTAGATGTGGCACTG
GCGGTAGATGCTGATGGTGTTCACGTCGGTCAGGATGATATGCCGGTGGCACTGGCGCGACGATTGATCGGCAACAAACT
GTTGGGAGTGTCGGCCCACAATCTCAGTGAAGCACTTCAGGCGGTGCGTGATGGGGCAGATTATCTCGGCGTTGGGCCGA
TCTTCGCCACAACAACCAAACCAGATGCCGCAGCGCCGATAGGCCTGGATGGTTTACGCGCCATCCGGCAACACGTATCG
ATCCCAATTGTGGCAATTGGCGGTATTAATCAGGCAAATGCTGCTGATGTGATGCGGGCAGGTGCTGATGGAATTGCTGT
TGTGTCAGCAGTAGTCGCAGCAGATGATGTCACTGCCGCTGCTCGTCAATTGCGTGCGCTGGTGTCAGTTACTCAGGAGA
AGGCGCTATGA

Upstream 100 bases:

>100_bases
TTACGATGCTATCTACCATCTTTCGGCTGATCAGATTCGTGCCGGGGCACGGGTAACTGCTGTTGCAGGGTAGTTTCCCT
GATAAGGAAAGGATCTGCCT

Downstream 100 bases:

>100_bases
CGTTACCGCGTTGTCTGACGATTGCCGGTTCTGATTCTGGTGGTGGGGCCGGTATTCAGGCCGATTTAAAAGCATTTGCG
GCCCTGGGTGCGTATGGTAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSIAHIVDWRLYVVTDAGLSRGRSHRAVIEAAIVGGATVVQYREKHASTRQMIEEALELRDLTRRAGVPLIVNDRVDVAL
AVDADGVHVGQDDMPVALARRLIGNKLLGVSAHNLSEALQAVRDGADYLGVGPIFATTTKPDAAAPIGLDGLRAIRQHVS
IPIVAIGGINQANAADVMRAGADGIAVVSAVVAADDVTAAARQLRALVSVTQEKAL

Sequences:

>Translated_216_residues
MSIAHIVDWRLYVVTDAGLSRGRSHRAVIEAAIVGGATVVQYREKHASTRQMIEEALELRDLTRRAGVPLIVNDRVDVAL
AVDADGVHVGQDDMPVALARRLIGNKLLGVSAHNLSEALQAVRDGADYLGVGPIFATTTKPDAAAPIGLDGLRAIRQHVS
IPIVAIGGINQANAADVMRAGADGIAVVSAVVAADDVTAAARQLRALVSVTQEKAL
>Mature_215_residues
SIAHIVDWRLYVVTDAGLSRGRSHRAVIEAAIVGGATVVQYREKHASTRQMIEEALELRDLTRRAGVPLIVNDRVDVALA
VDADGVHVGQDDMPVALARRLIGNKLLGVSAHNLSEALQAVRDGADYLGVGPIFATTTKPDAAAPIGLDGLRAIRQHVSI
PIVAIGGINQANAADVMRAGADGIAVVSAVVAADDVTAAARQLRALVSVTQEKAL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=188, Percent_Identity=35.1063829787234, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=227, Percent_Identity=40.0881057268723, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CHLAA (A9WDL8)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634013.1
- ProteinModelPortal:   A9WDL8
- SMR:   A9WDL8
- GeneID:   5825777
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0374
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   CLSK943643
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22596; Mature: 22465

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAHIVDWRLYVVTDAGLSRGRSHRAVIEAAIVGGATVVQYREKHASTRQMIEEALELR
CCCCCEECEEEEEEECCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DLTRRAGVPLIVNDRVDVALAVDADGVHVGQDDMPVALARRLIGNKLLGVSAHNLSEALQ
HHHHHCCCCEEEECCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCEECCCHHHHHHHHH
AVRDGADYLGVGPIFATTTKPDAAAPIGLDGLRAIRQHVSIPIVAIGGINQANAADVMRA
HHHCCCCCEECCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHC
GADGIAVVSAVVAADDVTAAARQLRALVSVTQEKAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIAHIVDWRLYVVTDAGLSRGRSHRAVIEAAIVGGATVVQYREKHASTRQMIEEALELR
CCCCEECEEEEEEECCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DLTRRAGVPLIVNDRVDVALAVDADGVHVGQDDMPVALARRLIGNKLLGVSAHNLSEALQ
HHHHHCCCCEEEECCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCEECCCHHHHHHHHH
AVRDGADYLGVGPIFATTTKPDAAAPIGLDGLRAIRQHVSIPIVAIGGINQANAADVMRA
HHHCCCCCEECCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHC
GADGIAVVSAVVAADDVTAAARQLRALVSVTQEKAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA