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The map label for this gene is thiDE [H]

Identifier: 222523693

GI number: 222523693

Start: 472050

End: 472850

Strand: Reverse

Name: thiDE [H]

Synonym: Chy400_0399

Alternate gene names: 222523693

Gene position: 472850-472050 (Counterclockwise)

Preceding gene: 222523694

Following gene: 222523691

Centisome position: 8.97

GC content: 56.18

Gene sequence:

>801_bases
ATGACGTTACCGCGTTGTCTGACGATTGCCGGTTCTGATTCTGGTGGTGGGGCCGGTATTCAGGCCGATTTAAAAGCATT
TGCGGCCCTGGGTGCGTATGGTATGAGCGTACTGACCGCCGTGACGGCTCAGAATACGGTGGGGGTGCAAGGGGTGGTGG
AATTGCCGGCAGCGTTTGTCGGTCAACAGATCGACTCGGTCGTTACCGACATCGGCGTCGATGCGGTGAAGACCGGGATG
CTGGCAAATGCCGACATTATTGCGGTGGTTGCTGAGAAGGCACGAGCCTATCAGTGGCCTTACCTGGTGGTTGATCCGGT
GATGGTAGCCAAGAGTGGACATCCATTGTTGCACCCTGAGGCGCAGCAGGCTTTGATCACGCTGTTGTTGCCACTGGCTA
CCATCGTGACCCCGAATCTCCCGGAAGCACGGGCACTAACCGGGCTTAACATTACAACTATTGCTGAGATGGAACAGGCG
GCACAGATGATCCATGCCATGGGGCCGCGCTGGGTACTGGTGAAAGGTGGACATCTCGAAGGTGATAGTGTTGATGTGCT
CTTCGACGGGGCGCAGTATCAGTACTTTACGGCGCCACGGATAGAGACACGACACACTCACGGAACCGGTTGTACTTTCG
CCTCGGCGATTGCTGCCGGTTTAGCAAAGGGTCTGTCGGTACCTGATGCAGTTGCACAGGCGAAAGAATACATTACCACC
GCTCTCCGTCATGCACCAGGTTTAGGTCAAGGGCATGGCCCGGTACACCATTTTGCCGCGTGGTATGGGGTTGCTGAATA
G

Upstream 100 bases:

>100_bases
ATGGAATTGCTGTTGTGTCAGCAGTAGTCGCAGCAGATGATGTCACTGCCGCTGCTCGTCAATTGCGTGCGCTGGTGTCA
GTTACTCAGGAGAAGGCGCT

Downstream 100 bases:

>100_bases
CAAACTGATACCGAAAGCAAAAGAAATTGAGGGAAGTGTATGCAACCAGAGAGCATCACTTCCCTCAATGAGCGTCAATT
CACGCAGAGAAAACAACGTT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTLPRCLTIAGSDSGGGAGIQADLKAFAALGAYGMSVLTAVTAQNTVGVQGVVELPAAFVGQQIDSVVTDIGVDAVKTGM
LANADIIAVVAEKARAYQWPYLVVDPVMVAKSGHPLLHPEAQQALITLLLPLATIVTPNLPEARALTGLNITTIAEMEQA
AQMIHAMGPRWVLVKGGHLEGDSVDVLFDGAQYQYFTAPRIETRHTHGTGCTFASAIAAGLAKGLSVPDAVAQAKEYITT
ALRHAPGLGQGHGPVHHFAAWYGVAE

Sequences:

>Translated_266_residues
MTLPRCLTIAGSDSGGGAGIQADLKAFAALGAYGMSVLTAVTAQNTVGVQGVVELPAAFVGQQIDSVVTDIGVDAVKTGM
LANADIIAVVAEKARAYQWPYLVVDPVMVAKSGHPLLHPEAQQALITLLLPLATIVTPNLPEARALTGLNITTIAEMEQA
AQMIHAMGPRWVLVKGGHLEGDSVDVLFDGAQYQYFTAPRIETRHTHGTGCTFASAIAAGLAKGLSVPDAVAQAKEYITT
ALRHAPGLGQGHGPVHHFAAWYGVAE
>Mature_265_residues
TLPRCLTIAGSDSGGGAGIQADLKAFAALGAYGMSVLTAVTAQNTVGVQGVVELPAAFVGQQIDSVVTDIGVDAVKTGML
ANADIIAVVAEKARAYQWPYLVVDPVMVAKSGHPLLHPEAQQALITLLLPLATIVTPNLPEARALTGLNITTIAEMEQAA
QMIHAMGPRWVLVKGGHLEGDSVDVLFDGAQYQYFTAPRIETRHTHGTGCTFASAIAAGLAKGLSVPDAVAQAKEYITTA
LRHAPGLGQGHGPVHHFAAWYGVAE

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=51.5384615384615, Blast_Score=240, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6325378, Length=274, Percent_Identity=35.4014598540146, Blast_Score=148, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6324997, Length=277, Percent_Identity=38.6281588447653, Blast_Score=135, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=38.2671480144404, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27619; Mature: 27488

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRCLTIAGSDSGGGAGIQADLKAFAALGAYGMSVLTAVTAQNTVGVQGVVELPAAFV
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GQQIDSVVTDIGVDAVKTGMLANADIIAVVAEKARAYQWPYLVVDPVMVAKSGHPLLHPE
HHHHHHHHHHHCHHHHHHCCCCCCHHHEEEHHHHHHHCCCEEEECCEEEECCCCCCCCCH
AQQALITLLLPLATIVTPNLPEARALTGLNITTIAEMEQAAQMIHAMGPRWVLVKGGHLE
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GDSVDVLFDGAQYQYFTAPRIETRHTHGTGCTFASAIAAGLAKGLSVPDAVAQAKEYITT
CCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALRHAPGLGQGHGPVHHFAAWYGVAE
HHHHCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TLPRCLTIAGSDSGGGAGIQADLKAFAALGAYGMSVLTAVTAQNTVGVQGVVELPAAFV
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GQQIDSVVTDIGVDAVKTGMLANADIIAVVAEKARAYQWPYLVVDPVMVAKSGHPLLHPE
HHHHHHHHHHHCHHHHHHCCCCCCHHHEEEHHHHHHHCCCEEEECCEEEECCCCCCCCCH
AQQALITLLLPLATIVTPNLPEARALTGLNITTIAEMEQAAQMIHAMGPRWVLVKGGHLE
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GDSVDVLFDGAQYQYFTAPRIETRHTHGTGCTFASAIAAGLAKGLSVPDAVAQAKEYITT
CCCEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALRHAPGLGQGHGPVHHFAAWYGVAE
HHHHCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA