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The map label for this gene is thiM

Identifier: 222523695

GI number: 222523695

Start: 473525

End: 474334

Strand: Reverse

Name: thiM

Synonym: Chy400_0401

Alternate gene names: 222523695

Gene position: 474334-473525 (Counterclockwise)

Preceding gene: 222523696

Following gene: 222523694

Centisome position: 9.0

GC content: 60.12

Gene sequence:

>810_bases
ATGACTCTGAACGAGCGGATCGCGGAGTTGCGCGAACGGGTGCGCCAGCAGCGCCCCCTCATTCACCACATTACCAATTT
TGTGGTGATGAATGATACGGCCAATGTAACGCTGCACATCGGTGGGTTGCCGGTTATGGCGCATGATCGAGCCGAGGTGG
CCGAGATGGTGACCGCTGCAGGTGCGCTGGTGTTGAATGTGGGCACGTTGTCGCCTGATTGGATTGAAGCGATGTTGATT
GCCGGTCGGCGGGCAAACGAGCTGGGGATTCCTATCGTGCTCGATCCGGTGGGTGCTGGTGCGACAAGCCTGCGCACGGC
GAGTAATCGGCGGTTGCTTGAAGAGCTTCAGATTGCGGTGGTGCGGGGTAATTCCGGCGAGATCGGTGCGCTGGCCGGGA
TGGGTGGCGTGGTCAAAGGGGTCGAGACGGTGGTTGAGGTCGATGATCCGACCGCAGCCGCGAAGGCGCTGGCCCAACAG
TACCGTACTGTGGTTGCTGTTACCGGTCGGCAAGATGTCGTGACCGATGGGAAGCGGGTGTTTCTGGTCGATAATGGTCA
TGAATGGCTGAAGACGCTCACCGGCACTGGCTGTTCGGCAACCACGGTTATCGCTGCATTTGCTGCAGTTGAGCGCGAGT
ATCCCTTTGCCGCAGCGGCTGCGCTGGCCTGTTTTGGTCTGGCCGCCGAACTGGCCGCGCCGGCAGCACGTGGGCCGGCA
AGCTTTAAGGTGGCCTTTTACGATGCTATCTACCATCTTTCGGCTGATCAGATTCGTGCCGGGGCACGGGTAACTGCTGT
TGCAGGGTAG

Upstream 100 bases:

>100_bases
GCACCGTCTGCATTGCGGAATAACCGTCCTGCCGCGGCAGGGCGGTTTTTTGCTACCTGCCCAATGATGTTCCGCCGTCT
GCTGGCGCAAGGAGGCCATG

Downstream 100 bases:

>100_bases
TTTCCCTGATAAGGAAAGGATCTGCCTGTGAGTATCGCCCATATTGTTGACTGGCGGCTGTATGTCGTCACTGATGCCGG
GCTGTCGCGAGGCCGTTCAC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTLNERIAELRERVRQQRPLIHHITNFVVMNDTANVTLHIGGLPVMAHDRAEVAEMVTAAGALVLNVGTLSPDWIEAMLI
AGRRANELGIPIVLDPVGAGATSLRTASNRRLLEELQIAVVRGNSGEIGALAGMGGVVKGVETVVEVDDPTAAAKALAQQ
YRTVVAVTGRQDVVTDGKRVFLVDNGHEWLKTLTGTGCSATTVIAAFAAVEREYPFAAAAALACFGLAAELAAPAARGPA
SFKVAFYDAIYHLSADQIRAGARVTAVAG

Sequences:

>Translated_269_residues
MTLNERIAELRERVRQQRPLIHHITNFVVMNDTANVTLHIGGLPVMAHDRAEVAEMVTAAGALVLNVGTLSPDWIEAMLI
AGRRANELGIPIVLDPVGAGATSLRTASNRRLLEELQIAVVRGNSGEIGALAGMGGVVKGVETVVEVDDPTAAAKALAQQ
YRTVVAVTGRQDVVTDGKRVFLVDNGHEWLKTLTGTGCSATTVIAAFAAVEREYPFAAAAALACFGLAAELAAPAARGPA
SFKVAFYDAIYHLSADQIRAGARVTAVAG
>Mature_268_residues
TLNERIAELRERVRQQRPLIHHITNFVVMNDTANVTLHIGGLPVMAHDRAEVAEMVTAAGALVLNVGTLSPDWIEAMLIA
GRRANELGIPIVLDPVGAGATSLRTASNRRLLEELQIAVVRGNSGEIGALAGMGGVVKGVETVVEVDDPTAAAKALAQQY
RTVVAVTGRQDVVTDGKRVFLVDNGHEWLKTLTGTGCSATTVIAAFAAVEREYPFAAAAALACFGLAAELAAPAARGPAS
FKVAFYDAIYHLSADQIRAGARVTAVAG

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=242, Percent_Identity=35.9504132231405, Blast_Score=131, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325042, Length=276, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_CHLAA (A9WDL9)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634014.1
- ProteinModelPortal:   A9WDL9
- SMR:   A9WDL9
- GeneID:   5825778
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0375
- HOGENOM:   HBG351126
- OMA:   MGICGEL
- ProtClustDB:   PRK09355
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28200; Mature: 28069

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 46-46 BINDING 122-122 BINDING 168-168 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNERIAELRERVRQQRPLIHHITNFVVMNDTANVTLHIGGLPVMAHDRAEVAEMVTAA
CCHHHHHHHHHHHHHHHCCHHHHHHCEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHC
GALVLNVGTLSPDWIEAMLIAGRRANELGIPIVLDPVGAGATSLRTASNRRLLEELQIAV
CEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEEEE
VRGNSGEIGALAGMGGVVKGVETVVEVDDPTAAAKALAQQYRTVVAVTGRQDVVTDGKRV
EECCCCCEEEECCCCHHHHHHHHEEECCCCHHHHHHHHHHHHEEEEECCCCCEECCCCEE
FLVDNGHEWLKTLTGTGCSATTVIAAFAAVEREYPFAAAAALACFGLAAELAAPAARGPA
EEEECCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
SFKVAFYDAIYHLSADQIRAGARVTAVAG
CEEEEEEHHHHHCCHHHHHCCCEEEEECC
>Mature Secondary Structure 
TLNERIAELRERVRQQRPLIHHITNFVVMNDTANVTLHIGGLPVMAHDRAEVAEMVTAA
CHHHHHHHHHHHHHHHCCHHHHHHCEEEECCCCEEEEEECCEEEEECCHHHHHHHHHHC
GALVLNVGTLSPDWIEAMLIAGRRANELGIPIVLDPVGAGATSLRTASNRRLLEELQIAV
CEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHEEEE
VRGNSGEIGALAGMGGVVKGVETVVEVDDPTAAAKALAQQYRTVVAVTGRQDVVTDGKRV
EECCCCCEEEECCCCHHHHHHHHEEECCCCHHHHHHHHHHHHEEEEECCCCCEECCCCEE
FLVDNGHEWLKTLTGTGCSATTVIAAFAAVEREYPFAAAAALACFGLAAELAAPAARGPA
EEEECCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
SFKVAFYDAIYHLSADQIRAGARVTAVAG
CEEEEEEHHHHHCCHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA