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The map label for this gene is yngG [H]

Identifier: 222523691

GI number: 222523691

Start: 470191

End: 471132

Strand: Reverse

Name: yngG [H]

Synonym: Chy400_0397

Alternate gene names: 222523691

Gene position: 471132-470191 (Counterclockwise)

Preceding gene: 222523693

Following gene: 222523690

Centisome position: 8.94

GC content: 57.64

Gene sequence:

>942_bases
ATGACCACAATGACAATTGGCACGCTACCGACGTTTGTGCATATTCGTGAAGTAGGGCCGCGTGATGGTTTGCAGAATGA
GCCAACGATTCTGACGACGGCGCAGAAGATAACGTTGATTGAGTTGTTGGCAGCGACCGGGTTGCGTGCGATTGAAGTAG
GGGCATTTGTGCGTCCGCAACAAGTGCCGCAGATGGCCGATACGGAAGCTGTCTTTGCCGGTATCAAGCGCCAGCCTGGG
GTAGTGTACAGTGCAATTGCGCCGAATGTTATCGGAGCGCGGCGGGCAATTGCCGCCGGTGCAGATGTTGTGCAGGTGTT
TCTCAGCGCCAGCGAGAGCCATAATCGCAGTAATGTGAATATGAGTATCGAGCAATCACTGGTGCAGGTGGCCGAGATGG
CGACGCTGGTGCATGCAGCCGGGAAGCCATTTGATGCGGTGCTGTCGGTGGCCTTCGGCTGCCCGTTTGAAGGGGATGTG
CCGATTGAGCGGGTGTTGGATCTGTGTCAGCGCTTGCTCGATTTGGGTGCCGAGCAGTTGACGCTGGGTGATACCACCGG
CATGGCTCACCCGCTGTTGGTGCAAGAGGTTGTCAGGGCGTTTCGCGCACGTTTTCCGCGCCAGCCCTTGCGTTTGCATT
TGCACAGTGCGCGGGGGGCGGGTCTGGCAAACCTGCTGGCAGCGTTGCAATTGGGTGTCGATCTGTTTGACTCTAGCATT
GGTGGTATTGGTGGTTGTCCGTTTGCGCCCGGCGCTCCTGGAAATCTCTGCACCGAAGATGTCACGCATTTGCTGCACGA
GATGGGGATTGCGACCGGGTTGAATCTGCCGGCACTCATGGCGACGGCGCGTGAACTTGAACGCATGTTAGGGCACGAAG
TGCCGGGACAGACGATTAAAGCCGGTATTTGTAAGCATCTGCGGGACCGCGGCGAGGGTTGA

Upstream 100 bases:

>100_bases
TGCTGAGTCATACATAGTGCCGATCATGACGATTGCAGGCTGCCAACAGTGATTGCCGGCAACATGAACCGTTCAAAACC
AGCAACCAGGGAGGTCGATG

Downstream 100 bases:

>100_bases
ATATGGCAGGTACACATTATGGATCCGCGTGATCGGGTGGTGATTATTACCGGTGCCTCAAGTGGGATTGGGGCGGCGAC
GGCTCGTTGTTTTGCGGCTG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTTMTIGTLPTFVHIREVGPRDGLQNEPTILTTAQKITLIELLAATGLRAIEVGAFVRPQQVPQMADTEAVFAGIKRQPG
VVYSAIAPNVIGARRAIAAGADVVQVFLSASESHNRSNVNMSIEQSLVQVAEMATLVHAAGKPFDAVLSVAFGCPFEGDV
PIERVLDLCQRLLDLGAEQLTLGDTTGMAHPLLVQEVVRAFRARFPRQPLRLHLHSARGAGLANLLAALQLGVDLFDSSI
GGIGGCPFAPGAPGNLCTEDVTHLLHEMGIATGLNLPALMATARELERMLGHEVPGQTIKAGICKHLRDRGEG

Sequences:

>Translated_313_residues
MTTMTIGTLPTFVHIREVGPRDGLQNEPTILTTAQKITLIELLAATGLRAIEVGAFVRPQQVPQMADTEAVFAGIKRQPG
VVYSAIAPNVIGARRAIAAGADVVQVFLSASESHNRSNVNMSIEQSLVQVAEMATLVHAAGKPFDAVLSVAFGCPFEGDV
PIERVLDLCQRLLDLGAEQLTLGDTTGMAHPLLVQEVVRAFRARFPRQPLRLHLHSARGAGLANLLAALQLGVDLFDSSI
GGIGGCPFAPGAPGNLCTEDVTHLLHEMGIATGLNLPALMATARELERMLGHEVPGQTIKAGICKHLRDRGEG
>Mature_312_residues
TTMTIGTLPTFVHIREVGPRDGLQNEPTILTTAQKITLIELLAATGLRAIEVGAFVRPQQVPQMADTEAVFAGIKRQPGV
VYSAIAPNVIGARRAIAAGADVVQVFLSASESHNRSNVNMSIEQSLVQVAEMATLVHAAGKPFDAVLSVAFGCPFEGDVP
IERVLDLCQRLLDLGAEQLTLGDTTGMAHPLLVQEVVRAFRARFPRQPLRLHLHSARGAGLANLLAALQLGVDLFDSSIG
GIGGCPFAPGAPGNLCTEDVTHLLHEMGIATGLNLPALMATARELERMLGHEVPGQTIKAGICKHLRDRGEG

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=305, Percent_Identity=40, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI109150422, Length=278, Percent_Identity=42.4460431654676, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI109150427, Length=278, Percent_Identity=42.4460431654676, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=37.5, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17510563, Length=281, Percent_Identity=35.5871886120996, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24582381, Length=280, Percent_Identity=39.6428571428571, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 33172; Mature: 33041

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMTIGTLPTFVHIREVGPRDGLQNEPTILTTAQKITLIELLAATGLRAIEVGAFVRPQ
CCEEEECCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHCCCCCHH
QVPQMADTEAVFAGIKRQPGVVYSAIAPNVIGARRAIAAGADVVQVFLSASESHNRSNVN
HCCCHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MSIEQSLVQVAEMATLVHAAGKPFDAVLSVAFGCPFEGDVPIERVLDLCQRLLDLGAEQL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHE
TLGDTTGMAHPLLVQEVVRAFRARFPRQPLRLHLHSARGAGLANLLAALQLGVDLFDSSI
ECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHCC
GGIGGCPFAPGAPGNLCTEDVTHLLHEMGIATGLNLPALMATARELERMLGHEVPGQTIK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
AGICKHLRDRGEG
HHHHHHHHHCCCC
>Mature Secondary Structure 
TTMTIGTLPTFVHIREVGPRDGLQNEPTILTTAQKITLIELLAATGLRAIEVGAFVRPQ
CEEEECCCHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCHHHHHCCCCCHH
QVPQMADTEAVFAGIKRQPGVVYSAIAPNVIGARRAIAAGADVVQVFLSASESHNRSNVN
HCCCHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MSIEQSLVQVAEMATLVHAAGKPFDAVLSVAFGCPFEGDVPIERVLDLCQRLLDLGAEQL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHE
TLGDTTGMAHPLLVQEVVRAFRARFPRQPLRLHLHSARGAGLANLLAALQLGVDLFDSSI
ECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHCC
GGIGGCPFAPGAPGNLCTEDVTHLLHEMGIATGLNLPALMATARELERMLGHEVPGQTIK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
AGICKHLRDRGEG
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]