Click here to switch to the map view.

The map label for this gene is fabH [C]

Identifier: 222523544

GI number: 222523544

Start: 296337

End: 297386

Strand: Reverse

Name: fabH [C]

Synonym: Chy400_0250

Alternate gene names: 222523544

Gene position: 297386-296337 (Counterclockwise)

Preceding gene: 222523545

Following gene: 222523543

Centisome position: 5.64

GC content: 60.48

Gene sequence:

>1050_bases
ATGATGAAACCACAGCATCCTGTCGGTATTGTCGGCTACGGTGCCTATATCCCGCGGTACCGGATTGCTGCCCGCGAGAT
CGCCCGGGTCTGGACTGATGGGCAGAGTGGAACGCCAATCGAGGCGAAGAGTGTTCCGGGTCCTGACGAAGATACGATTA
CGATGTCTATTGAAGCGGCTCGCAATGCGCTGGCCCGGGCCGCCATCCCGGCGACGGCGTTGGGTGCAGTCTGGATCGGT
AGTGAGAGCCATCCGTACAGCGTGAAACCTTCAGGGACGGTGGTTGCCGATGCGCTGGGGGCCGGCCCCTGGGTAAGCGC
CGCTGACTGGGAGTTTGCCTGCAAAGCCGGTTCTGAAGCGCTGACCGCAGCGATGGCGCTGGTTGGCAGTGGTATGCAAC
GCTATGCGCTGGCCGTCGGCGCCGATACAGCGCAGGGCCGCCCCGGTGATGCGCTGGAATACACTGCTTCCGCCGGTGCC
GCAGCGTTGATCGTTGGTCCGGCCAGCGAGGCGCTGGCAACCATTGATGCCACGTTGTCATACGTGACCGATACGCCCGA
CTTTTACCGTCGCGCCGATCGGCCCTACCCGGTGCACGGTAATCGCTTCACCGGCGAACCGGCTTACTTCCATCAGATTC
AGTCAGCAGCTACCGAATTGTTACGCCATCTCAACCGCACCGCAGCCGATTTCACCTATGCCGTCTTTCATCAACCCAAT
GCCAAGTTTCCGCAAGCGGTTGCCAAACGCCTGGGTTTCACCGATAAACAGATTGCCCCCGGCTTGCTCAGCCCTCAGAT
CGGTAACACCTATTCCGGGGCAGCGCTGCTTGGTCTCTGCGCTATCCTCGATGTGGCCCAACCGGGTGAGACGATTTTTA
TGACGACCTACGGCAGTGGGGCCGGTTCCGATGCCTATGCACTTACTGTTACCGAAGCCGTTCTGGAACGTCGTGAGCGG
GCACCGCTTGTCGCGGCCTACCTGCAACGGGCAGTGATGATTGATTACGCCATCTATGCGAAGTGGCGCGGTAAGCTGGT
GATGGGATAG

Upstream 100 bases:

>100_bases
CTCTGTTTCCCGATAACAGATGAGGATTCGGTACGTATTGGTTCGCCTGCGGAAGGCGAGCCGGTCTGGGTATGCCTGAC
ATCTGCATGGAGTACAGTTC

Downstream 100 bases:

>100_bases
TCTATCAGGAGTGCAACAATGACCAATGTCTATATTGCAGGGATAGGGGCAACCGCCGTTGGCGAACATTATCGCCGTGG
GCTGGCCGATCTGGTGAGTG

Product: hypothetical protein

Products: acetoacetyl-ACP; Coenzyme A; CO2; acetyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MMKPQHPVGIVGYGAYIPRYRIAAREIARVWTDGQSGTPIEAKSVPGPDEDTITMSIEAARNALARAAIPATALGAVWIG
SESHPYSVKPSGTVVADALGAGPWVSAADWEFACKAGSEALTAAMALVGSGMQRYALAVGADTAQGRPGDALEYTASAGA
AALIVGPASEALATIDATLSYVTDTPDFYRRADRPYPVHGNRFTGEPAYFHQIQSAATELLRHLNRTAADFTYAVFHQPN
AKFPQAVAKRLGFTDKQIAPGLLSPQIGNTYSGAALLGLCAILDVAQPGETIFMTTYGSGAGSDAYALTVTEAVLERRER
APLVAAYLQRAVMIDYAIYAKWRGKLVMG

Sequences:

>Translated_349_residues
MMKPQHPVGIVGYGAYIPRYRIAAREIARVWTDGQSGTPIEAKSVPGPDEDTITMSIEAARNALARAAIPATALGAVWIG
SESHPYSVKPSGTVVADALGAGPWVSAADWEFACKAGSEALTAAMALVGSGMQRYALAVGADTAQGRPGDALEYTASAGA
AALIVGPASEALATIDATLSYVTDTPDFYRRADRPYPVHGNRFTGEPAYFHQIQSAATELLRHLNRTAADFTYAVFHQPN
AKFPQAVAKRLGFTDKQIAPGLLSPQIGNTYSGAALLGLCAILDVAQPGETIFMTTYGSGAGSDAYALTVTEAVLERRER
APLVAAYLQRAVMIDYAIYAKWRGKLVMG
>Mature_349_residues
MMKPQHPVGIVGYGAYIPRYRIAAREIARVWTDGQSGTPIEAKSVPGPDEDTITMSIEAARNALARAAIPATALGAVWIG
SESHPYSVKPSGTVVADALGAGPWVSAADWEFACKAGSEALTAAMALVGSGMQRYALAVGADTAQGRPGDALEYTASAGA
AALIVGPASEALATIDATLSYVTDTPDFYRRADRPYPVHGNRFTGEPAYFHQIQSAATELLRHLNRTAADFTYAVFHQPN
AKFPQAVAKRLGFTDKQIAPGLLSPQIGNTYSGAALLGLCAILDVAQPGETIFMTTYGSGAGSDAYALTVTEAVLERRER
APLVAAYLQRAVMIDYAIYAKWRGKLVMG

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013751
- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08545 ACP_syn_III; PF08541 ACP_syn_III_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 36842; Mature: 36842

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKPQHPVGIVGYGAYIPRYRIAAREIARVWTDGQSGTPIEAKSVPGPDEDTITMSIEAA
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEHHHH
RNALARAAIPATALGAVWIGSESHPYSVKPSGTVVADALGAGPWVSAADWEFACKAGSEA
HHHHHHHHCCHHHHCEEEECCCCCCEEECCCCCEEEHHCCCCCCCCCCCCHHHHHCCHHH
LTAAMALVGSGMQRYALAVGADTAQGRPGDALEYTASAGAAALIVGPASEALATIDATLS
HHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHH
YVTDTPDFYRRADRPYPVHGNRFTGEPAYFHQIQSAATELLRHLNRTAADFTYAVFHQPN
HHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
AKFPQAVAKRLGFTDKQIAPGLLSPQIGNTYSGAALLGLCAILDVAQPGETIFMTTYGSG
CCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCC
AGSDAYALTVTEAVLERRERAPLVAAYLQRAVMIDYAIYAKWRGKLVMG
CCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCEEECC
>Mature Secondary Structure
MMKPQHPVGIVGYGAYIPRYRIAAREIARVWTDGQSGTPIEAKSVPGPDEDTITMSIEAA
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEHHHH
RNALARAAIPATALGAVWIGSESHPYSVKPSGTVVADALGAGPWVSAADWEFACKAGSEA
HHHHHHHHCCHHHHCEEEECCCCCCEEECCCCCEEEHHCCCCCCCCCCCCHHHHHCCHHH
LTAAMALVGSGMQRYALAVGADTAQGRPGDALEYTASAGAAALIVGPASEALATIDATLS
HHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHH
YVTDTPDFYRRADRPYPVHGNRFTGEPAYFHQIQSAATELLRHLNRTAADFTYAVFHQPN
HHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
AKFPQAVAKRLGFTDKQIAPGLLSPQIGNTYSGAALLGLCAILDVAQPGETIFMTTYGSG
CCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCC
AGSDAYALTVTEAVLERRERAPLVAAYLQRAVMIDYAIYAKWRGKLVMG
CCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-CoA; malonyl-ACP; acetyl-CoA [C]

Specific reaction: acetyl-CoA + malonyl-ACP = acetoacetyl-ACP + Coenzyme A + CO2 [acyl carrier protein] + acetyl-CoA = acetyl-ACP + Coenzyme A [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA