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The map label for this gene is leuD [H]

Identifier: 222109205

GI number: 222109205

Start: 93081

End: 93779

Strand: Reverse

Name: leuD [H]

Synonym: Avi_9634

Alternate gene names: 222109205

Gene position: 93779-93081 (Counterclockwise)

Preceding gene: 222109206

Following gene: 222109204

Centisome position: 71.9

GC content: 52.07

Gene sequence:

>699_bases
ATGACGTCAATCCTCGTCGTCGGAACTGCTGTGCCTATCATTGAGGACAACGTCGACACGGACCAAATCACCCCTGGAAC
CGAGCTCATGCGTTCCGCTGATGACAGCTACTTGCGGTGGGGAGGCGCGCTGTTCGCTGAAAGAAGATACAATCTCGATG
GAAACCTGAACCCGGATTTTATCCTAAATCAGACCGAGTGGCTTGGCGCGACGTTCTTGTTGACTGGGAAGAACTTTGGG
TGCGGATCTTCCCGTGAATGGGCCGCTAAGGCGATTCGCGGGTATGGATTCAAGGCAATTCTCGCTGAGTCCTTCGGAGA
GATTTTCATAGGTAATTGTTACCGGCATGGCATAGCGCCAATCGTGCTTTCCCCCAATGAAATCGGCCAGCTACGACATG
TCGCGTTGCAGCACTCCCCGATCACCATTGATCTGGCCAAGCAGACGATTTCCACCTCGCCCGAGAAGAAGTTCGATTTC
AAAATACCAGAAATGTATCGCCGGATGCTTATCGAAGGAATCGATGAGATTTCCTTTGTGATGAGATTTGCACCATCGGT
GGAGAACTTTGAGAAGACTGATCGGTTGAAGCGGCCTTGGATGCATGTCACCCGGGCCAATAACGGGAGTAAGTGCTATG
GCAGCGAACGAGATTGCCGCAAACATTGTCACGAATGCAGTTGGATGGCGGCGAGCTGA

Upstream 100 bases:

>100_bases
GGACGATGACGCACCTCGCGAGCCCAGTAACAGTAGCCGCATCAGCAATCTTAGGCGGAATCGCAGATATAAGGAAAATG
GAAAATGCGGGGAGCGCCCT

Downstream 100 bases:

>100_bases
CACCCGAGATCCCTCAGCTTGGACATACACCATATCCAATGCGGAACGGGAAGAGATTGAAGTTGCATTCGCACAAGCTA
GTGAAAGCGGTCTGGACACC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTSILVVGTAVPIIEDNVDTDQITPGTELMRSADDSYLRWGGALFAERRYNLDGNLNPDFILNQTEWLGATFLLTGKNFG
CGSSREWAAKAIRGYGFKAILAESFGEIFIGNCYRHGIAPIVLSPNEIGQLRHVALQHSPITIDLAKQTISTSPEKKFDF
KIPEMYRRMLIEGIDEISFVMRFAPSVENFEKTDRLKRPWMHVTRANNGSKCYGSERDCRKHCHECSWMAAS

Sequences:

>Translated_232_residues
MTSILVVGTAVPIIEDNVDTDQITPGTELMRSADDSYLRWGGALFAERRYNLDGNLNPDFILNQTEWLGATFLLTGKNFG
CGSSREWAAKAIRGYGFKAILAESFGEIFIGNCYRHGIAPIVLSPNEIGQLRHVALQHSPITIDLAKQTISTSPEKKFDF
KIPEMYRRMLIEGIDEISFVMRFAPSVENFEKTDRLKRPWMHVTRANNGSKCYGSERDCRKHCHECSWMAAS
>Mature_231_residues
TSILVVGTAVPIIEDNVDTDQITPGTELMRSADDSYLRWGGALFAERRYNLDGNLNPDFILNQTEWLGATFLLTGKNFGC
GSSREWAAKAIRGYGFKAILAESFGEIFIGNCYRHGIAPIVLSPNEIGQLRHVALQHSPITIDLAKQTISTSPEKKFDFK
IPEMYRRMLIEGIDEISFVMRFAPSVENFEKTDRLKRPWMHVTRANNGSKCYGSERDCRKHCHECSWMAAS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=189, Percent_Identity=35.4497354497354, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=202, Percent_Identity=32.6732673267327, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 26186; Mature: 26055

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSILVVGTAVPIIEDNVDTDQITPGTELMRSADDSYLRWGGALFAERRYNLDGNLNPDF
CCEEEEEECCCEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCCE
ILNQTEWLGATFLLTGKNFGCGSSREWAAKAIRGYGFKAILAESFGEIFIGNCYRHGIAP
EECCHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEECCHHHCCCCE
IVLSPNEIGQLRHVALQHSPITIDLAKQTISTSPEKKFDFKIPEMYRRMLIEGIDEISFV
EEECCCHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHCHHHHHHH
MRFAPSVENFEKTDRLKRPWMHVTRANNGSKCYGSERDCRKHCHECSWMAAS
HHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSILVVGTAVPIIEDNVDTDQITPGTELMRSADDSYLRWGGALFAERRYNLDGNLNPDF
CEEEEEECCCEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCCE
ILNQTEWLGATFLLTGKNFGCGSSREWAAKAIRGYGFKAILAESFGEIFIGNCYRHGIAP
EECCHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEEECCHHHCCCCE
IVLSPNEIGQLRHVALQHSPITIDLAKQTISTSPEKKFDFKIPEMYRRMLIEGIDEISFV
EEECCCHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHCHHHHHHH
MRFAPSVENFEKTDRLKRPWMHVTRANNGSKCYGSERDCRKHCHECSWMAAS
HHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA