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The map label for this gene is leuC [H]

Identifier: 222109206

GI number: 222109206

Start: 93776

End: 95125

Strand: Reverse

Name: leuC [H]

Synonym: Avi_9636

Alternate gene names: 222109206

Gene position: 95125-93776 (Counterclockwise)

Preceding gene: 222109212

Following gene: 222109205

Centisome position: 72.93

GC content: 54.3

Gene sequence:

>1350_bases
CTGCTGTATATCGACCGAATTTTTTTACATGAACTAACTGGCGCCTCTGCTATGAGGCAACTGGAGAATGGAAGTCGCCG
GCCACGCCGGTCAGATTCAGTATTCGTTACCATCGACCACGTAATTGATACGGCACCTGGCCGCACGAAAAACCAATCAG
TTGCATCGCTAGGCTCCGCGATGATCCAAGAGACGACGGAGGCCGCCCGCCGATATGGGTTTACCCTTTTCGATGCCGAT
GATCGTCGCCAAGGAATCGTACACGTAATATCTCCCGACCTTGGAATAGCTTTGCCAGGCGTGACATTAGTTTGTGGCGA
TAGCCATACGAGTACTGTCGGTGGGGTTGGAGCGATTGGCTTCGGCATCGGCTCGACCGACTGTGAACAGATTTTGGCTT
CTCAGACACTGGCTTTGAAGCGGCCGAGAGCAATGCGCATCTGGGTGACCGGTATTCTGCAGAACGGTGTATACCCAAAG
GACCTCATTCTTGATCTTATAAGGAATATAGGGGCAAATGGAGGCCTTGGCTTCGCCGTAGAATTTGCAGGCCCCGCCAT
AGCGGCTATGTCTGTCGAGGGTCGGCTGACGCTCTGCAACATGGCAACAGAGTTCGGTGCGAAATTTGGCTTCGTGGCAC
CAGATGAGCAGACGTTCAACTATCTCGTGGGTAGGAACTATGCGCCCCATGGTCGGGCTTGGGATGAAGCTGTCGAATAT
TGGAAATCGCTGACGACAGATACGGACGCGACGTTCGACCGGGAAGTCAGCATTGATTGCGCCTGCCTTGAACCACAAAT
CACTTGGGGTACAAGCCCCCAGCACGTAGTAGGAGTTGCTTCGAAAGTGCCTGAGATTGAAGAGATCTCACCCGATCAAC
GGTCGTCGGCACTACGTGCGTTGGACTACATGCATCTCAAACCTGGGCAGCAGTTAGAAGGCCTCCCCGTTGATGCCGCA
TACATCGGTGCTTGCACGAATGCGCGGCTAGCGGATCTTCAAGCGGCTGGATCAGTTCTTCGAGGTCGCAGAGTTCACCC
CTCGGTCCTGGCGATCTGTGTTCCCGGATCAAGAGAAGTCAAGGAGAAAGCCGAAGCCGAGGGCCTACATGCTGTTTTCA
AATCAGCTGGCTTCGAGTGGCATGAAGCGGGGTGCGGGCTGTGTGGGCATATGGGCAATGATCGGCTCAAGGGTCTACGC
GTTGTTAGTACGACGAACCGAAACTTTGTCGGAAGACAGGGGCCTGGGACGATGACGCACCTCGCGAGCCCAGTAACAGT
AGCCGCATCAGCAATCTTAGGCGGAATCGCAGATATAAGGAAAATGGAAAATGCGGGGAGCGCCCTATGA

Upstream 100 bases:

>100_bases
ATCCAACCGATGATGGTGAGGCTCAGAGAAAATGGCCCGCACATTTTTTGAAAAAGTCTGGAACGATCACGTCGTTACCG
AAATCGATTCAGACACGTCT

Downstream 100 bases:

>100_bases
CGTCAATCCTCGTCGTCGGAACTGCTGTGCCTATCATTGAGGACAACGTCGACACGGACCAAATCACCCCTGGAACCGAG
CTCATGCGTTCCGCTGATGA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MLYIDRIFLHELTGASAMRQLENGSRRPRRSDSVFVTIDHVIDTAPGRTKNQSVASLGSAMIQETTEAARRYGFTLFDAD
DRRQGIVHVISPDLGIALPGVTLVCGDSHTSTVGGVGAIGFGIGSTDCEQILASQTLALKRPRAMRIWVTGILQNGVYPK
DLILDLIRNIGANGGLGFAVEFAGPAIAAMSVEGRLTLCNMATEFGAKFGFVAPDEQTFNYLVGRNYAPHGRAWDEAVEY
WKSLTTDTDATFDREVSIDCACLEPQITWGTSPQHVVGVASKVPEIEEISPDQRSSALRALDYMHLKPGQQLEGLPVDAA
YIGACTNARLADLQAAGSVLRGRRVHPSVLAICVPGSREVKEKAEAEGLHAVFKSAGFEWHEAGCGLCGHMGNDRLKGLR
VVSTTNRNFVGRQGPGTMTHLASPVTVAASAILGGIADIRKMENAGSAL

Sequences:

>Translated_449_residues
MLYIDRIFLHELTGASAMRQLENGSRRPRRSDSVFVTIDHVIDTAPGRTKNQSVASLGSAMIQETTEAARRYGFTLFDAD
DRRQGIVHVISPDLGIALPGVTLVCGDSHTSTVGGVGAIGFGIGSTDCEQILASQTLALKRPRAMRIWVTGILQNGVYPK
DLILDLIRNIGANGGLGFAVEFAGPAIAAMSVEGRLTLCNMATEFGAKFGFVAPDEQTFNYLVGRNYAPHGRAWDEAVEY
WKSLTTDTDATFDREVSIDCACLEPQITWGTSPQHVVGVASKVPEIEEISPDQRSSALRALDYMHLKPGQQLEGLPVDAA
YIGACTNARLADLQAAGSVLRGRRVHPSVLAICVPGSREVKEKAEAEGLHAVFKSAGFEWHEAGCGLCGHMGNDRLKGLR
VVSTTNRNFVGRQGPGTMTHLASPVTVAASAILGGIADIRKMENAGSAL
>Mature_449_residues
MLYIDRIFLHELTGASAMRQLENGSRRPRRSDSVFVTIDHVIDTAPGRTKNQSVASLGSAMIQETTEAARRYGFTLFDAD
DRRQGIVHVISPDLGIALPGVTLVCGDSHTSTVGGVGAIGFGIGSTDCEQILASQTLALKRPRAMRIWVTGILQNGVYPK
DLILDLIRNIGANGGLGFAVEFAGPAIAAMSVEGRLTLCNMATEFGAKFGFVAPDEQTFNYLVGRNYAPHGRAWDEAVEY
WKSLTTDTDATFDREVSIDCACLEPQITWGTSPQHVVGVASKVPEIEEISPDQRSSALRALDYMHLKPGQQLEGLPVDAA
YIGACTNARLADLQAAGSVLRGRRVHPSVLAICVPGSREVKEKAEAEGLHAVFKSAGFEWHEAGCGLCGHMGNDRLKGLR
VVSTTNRNFVGRQGPGTMTHLASPVTVAASAILGGIADIRKMENAGSAL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=27.7404921700224, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI8659555, Length=372, Percent_Identity=27.1505376344086, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=446, Percent_Identity=47.5336322869955, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787531, Length=369, Percent_Identity=28.7262872628726, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081781, Length=379, Percent_Identity=25.8575197889182, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=444, Percent_Identity=29.0540540540541, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=440, Percent_Identity=28.8636363636364, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=489, Percent_Identity=26.3803680981595, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=325, Percent_Identity=29.2307692307692, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=451, Percent_Identity=45.8980044345898, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=447, Percent_Identity=27.5167785234899, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320440, Length=424, Percent_Identity=27.122641509434, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=27.0089285714286, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=27.0089285714286, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161076999, Length=414, Percent_Identity=27.0531400966184, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28571643, Length=447, Percent_Identity=26.3982102908277, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17137564, Length=397, Percent_Identity=27.455919395466, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24645686, Length=373, Percent_Identity=27.6139410187668, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 48014; Mature: 48014

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIDRIFLHELTGASAMRQLENGSRRPRRSDSVFVTIDHVIDTAPGRTKNQSVASLGSA
CEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCCCCCHHHHHHHHH
MIQETTEAARRYGFTLFDADDRRQGIVHVISPDLGIALPGVTLVCGDSHTSTVGGVGAIG
HHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCEECCCEEEEECCCCCCCCCCCHHEE
FGIGSTDCEQILASQTLALKRPRAMRIWVTGILQNGVYPKDLILDLIRNIGANGGLGFAV
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCCEEEE
EFAGPAIAAMSVEGRLTLCNMATEFGAKFGFVAPDEQTFNYLVGRNYAPHGRAWDEAVEY
EECCCEEEEEEECCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
WKSLTTDTDATFDREVSIDCACLEPQITWGTSPQHVVGVASKVPEIEEISPDQRSSALRA
HHHHCCCCCCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHH
LDYMHLKPGQQLEGLPVDAAYIGACTNARLADLQAAGSVLRGRRVHPSVLAICVPGSREV
HHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
KEKAEAEGLHAVFKSAGFEWHEAGCGLCGHMGNDRLKGLRVVSTTNRNFVGRQGPGTMTH
HHHHHHHHHHHHHHHCCCCCHHCCCCHHCCCCCCHHCCEEEEEECCCCCCCCCCCCCHHH
LASPVTVAASAILGGIADIRKMENAGSAL
HCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLYIDRIFLHELTGASAMRQLENGSRRPRRSDSVFVTIDHVIDTAPGRTKNQSVASLGSA
CEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCCCCCCCCHHHHHHHHH
MIQETTEAARRYGFTLFDADDRRQGIVHVISPDLGIALPGVTLVCGDSHTSTVGGVGAIG
HHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCEECCCEEEEECCCCCCCCCCCHHEE
FGIGSTDCEQILASQTLALKRPRAMRIWVTGILQNGVYPKDLILDLIRNIGANGGLGFAV
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCCEEEE
EFAGPAIAAMSVEGRLTLCNMATEFGAKFGFVAPDEQTFNYLVGRNYAPHGRAWDEAVEY
EECCCEEEEEEECCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
WKSLTTDTDATFDREVSIDCACLEPQITWGTSPQHVVGVASKVPEIEEISPDQRSSALRA
HHHHCCCCCCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHH
LDYMHLKPGQQLEGLPVDAAYIGACTNARLADLQAAGSVLRGRRVHPSVLAICVPGSREV
HHHHCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHH
KEKAEAEGLHAVFKSAGFEWHEAGCGLCGHMGNDRLKGLRVVSTTNRNFVGRQGPGTMTH
HHHHHHHHHHHHHHHCCCCCHHCCCCHHCCCCCCHHCCEEEEEECCCCCCCCCCCCCHHH
LASPVTVAASAILGGIADIRKMENAGSAL
HCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]