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The map label for this gene is leuA1 [H]

Identifier: 222109189

GI number: 222109189

Start: 76409

End: 77671

Strand: Reverse

Name: leuA1 [H]

Synonym: Avi_9608

Alternate gene names: 222109189

Gene position: 77671-76409 (Counterclockwise)

Preceding gene: 222109190

Following gene: 222109188

Centisome position: 59.55

GC content: 52.49

Gene sequence:

>1263_bases
ATGTCAGATTTAATCCTGGAGACAGCGCTTAGCAATAGGGTTATCTTTCATGAAGAGGTGGCGAGGGACGGGGCGCAAGG
GAAGACGCTCCTGAATGGCCATCAGAGAGTTCAAATCGCAAAACGTCACAGCTCCATTTTCGGTGAATTCGCGCCTTATC
ACTTGGTCTTCAATGCTGGTTTCCCGAGTGCTGGCAAGGATGAGCTTGAAGCGACAAGGCAGGTCGTTGATTGCGTCGAC
GAATGCTATGTCGGAGCAGCTGGACGAGCAACTCGGCAAGATGTTGATTTGTTGCTCCAGGCGGTACACGGCGCGCGGCA
CGGTAGGATCTTCATTGCTGTGCCTGCCTCCAATCCTATGGCATATTCCATGATGCATTGTACGGCTGAGAAGGCAAAAC
AAGCGGCATTGGATATGGTAAGATATGCCAGAGATAAGAACCCAGCCTTGATGGTTGACGTGGCCCTTGCAGATGCATCT
CGTTCCGACGTGGGCTTTCTAGCCGCGTCGGCGGCCGAGTACGCCGCCGCCGGTGCATCAATGGTGATCGTATGTGACAC
GGTGGGGCAGTTTTTTCCGTCGGAAAGCCGATGGTTTTTTGACGAACTTGCTCGACGGTCTGATCCGGATTTGCGTTACG
TTGCTCACATGCACAACGATCTTGGCTTCGGCTTAGTCAATACACTTGAAGCTTTGTCTGCCAAAATATATGGCCTGACG
AGTTCATGGCTCTCTCTTGGAGAGCGGGCAGGAATGCCTGCAACAGAACAGTTACTGTTTGCGCTTGGCTATCAAAGCAG
TCTTTTGCCAAAAAAGTTGGGCGTCGTGGATGAGATATGGCGCTCACCACCTGATCTACGTCAGCTCTCGCCGATCGCAA
GGTACGTCAGTCAAAGTACACGAAGGCCTCTCATGACAACCGATCCCATTGTGGGAACAGGTGTGAACACGATTTCGACC
GGCACGCCATTCACGAATCCGGCTCTCTTTCAGCCGTTCGACCCCTTAGAGATACTTGGAGTTGAACCAAAGGTGCTGTT
GAGCTCTCTAGCAAGCCACCGCATAATTCATGCCGTTGCGGCTCGGCTAGGATGGGCGCTCGACCAGTCTCAGGCATCCG
CAGCTCTTGAATGGGTAAAGGCCGAGGCCTTCAGCCGCAATGTAGCTATTATTGAAGATGAAGACTTTGAGCATTTTTTA
AAGGGAGCGAACTTCGCCCAAACATCCGCCGCCGCGAAGGATCACCTTAGGATCTCTCGATGA

Upstream 100 bases:

>100_bases
AGGTTTGTCTGCTGCAGGCGCCGTGAAATGAATTGACGCGCCTAGAGGATCCCTGCGGTAACGACGGCGTGCAATCGTGA
AACCAAGAGCCGGAGGTAAC

Downstream 100 bases:

>100_bases
ACAAACCTCACGTTAGCACTGATCACACGACGTTTCATATCACATGAGGGTTAGGGTTTGATATGAGCAATTCATGCCAA
CTCGTTTTTCCATCTCACAT

Product: isopropylmalate/homocitrate/citramalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSDLILETALSNRVIFHEEVARDGAQGKTLLNGHQRVQIAKRHSSIFGEFAPYHLVFNAGFPSAGKDELEATRQVVDCVD
ECYVGAAGRATRQDVDLLLQAVHGARHGRIFIAVPASNPMAYSMMHCTAEKAKQAALDMVRYARDKNPALMVDVALADAS
RSDVGFLAASAAEYAAAGASMVIVCDTVGQFFPSESRWFFDELARRSDPDLRYVAHMHNDLGFGLVNTLEALSAKIYGLT
SSWLSLGERAGMPATEQLLFALGYQSSLLPKKLGVVDEIWRSPPDLRQLSPIARYVSQSTRRPLMTTDPIVGTGVNTIST
GTPFTNPALFQPFDPLEILGVEPKVLLSSLASHRIIHAVAARLGWALDQSQASAALEWVKAEAFSRNVAIIEDEDFEHFL
KGANFAQTSAAAKDHLRISR

Sequences:

>Translated_420_residues
MSDLILETALSNRVIFHEEVARDGAQGKTLLNGHQRVQIAKRHSSIFGEFAPYHLVFNAGFPSAGKDELEATRQVVDCVD
ECYVGAAGRATRQDVDLLLQAVHGARHGRIFIAVPASNPMAYSMMHCTAEKAKQAALDMVRYARDKNPALMVDVALADAS
RSDVGFLAASAAEYAAAGASMVIVCDTVGQFFPSESRWFFDELARRSDPDLRYVAHMHNDLGFGLVNTLEALSAKIYGLT
SSWLSLGERAGMPATEQLLFALGYQSSLLPKKLGVVDEIWRSPPDLRQLSPIARYVSQSTRRPLMTTDPIVGTGVNTIST
GTPFTNPALFQPFDPLEILGVEPKVLLSSLASHRIIHAVAARLGWALDQSQASAALEWVKAEAFSRNVAIIEDEDFEHFL
KGANFAQTSAAAKDHLRISR
>Mature_419_residues
SDLILETALSNRVIFHEEVARDGAQGKTLLNGHQRVQIAKRHSSIFGEFAPYHLVFNAGFPSAGKDELEATRQVVDCVDE
CYVGAAGRATRQDVDLLLQAVHGARHGRIFIAVPASNPMAYSMMHCTAEKAKQAALDMVRYARDKNPALMVDVALADASR
SDVGFLAASAAEYAAAGASMVIVCDTVGQFFPSESRWFFDELARRSDPDLRYVAHMHNDLGFGLVNTLEALSAKIYGLTS
SWLSLGERAGMPATEQLLFALGYQSSLLPKKLGVVDEIWRSPPDLRQLSPIARYVSQSTRRPLMTTDPIVGTGVNTISTG
TPFTNPALFQPFDPLEILGVEPKVLLSSLASHRIIHAVAARLGWALDQSQASAALEWVKAEAFSRNVAIIEDEDFEHFLK
GANFAQTSAAAKDHLRISR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=317, Percent_Identity=25.8675078864353, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 45797; Mature: 45665

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLILETALSNRVIFHEEVARDGAQGKTLLNGHQRVQIAKRHSSIFGEFAPYHLVFNAG
CCCHHHHHHHCCCEEEHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECC
FPSAGKDELEATRQVVDCVDECYVGAAGRATRQDVDLLLQAVHGARHGRIFIAVPASNPM
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
AYSMMHCTAEKAKQAALDMVRYARDKNPALMVDVALADASRSDVGFLAASAAEYAAAGAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
MVIVCDTVGQFFPSESRWFFDELARRSDPDLRYVAHMHNDLGFGLVNTLEALSAKIYGLT
EEEEECHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
SSWLSLGERAGMPATEQLLFALGYQSSLLPKKLGVVDEIWRSPPDLRQLSPIARYVSQST
HHHHHHHHHCCCCHHHHHHHHHCCCHHCCHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHH
RRPLMTTDPIVGTGVNTISTGTPFTNPALFQPFDPLEILGVEPKVLLSSLASHRIIHAVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
ARLGWALDQSQASAALEWVKAEAFSRNVAIIEDEDFEHFLKGANFAQTSAAAKDHLRISR
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDLILETALSNRVIFHEEVARDGAQGKTLLNGHQRVQIAKRHSSIFGEFAPYHLVFNAG
CCHHHHHHHCCCEEEHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECC
FPSAGKDELEATRQVVDCVDECYVGAAGRATRQDVDLLLQAVHGARHGRIFIAVPASNPM
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
AYSMMHCTAEKAKQAALDMVRYARDKNPALMVDVALADASRSDVGFLAASAAEYAAAGAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
MVIVCDTVGQFFPSESRWFFDELARRSDPDLRYVAHMHNDLGFGLVNTLEALSAKIYGLT
EEEEECHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
SSWLSLGERAGMPATEQLLFALGYQSSLLPKKLGVVDEIWRSPPDLRQLSPIARYVSQST
HHHHHHHHHCCCCHHHHHHHHHCCCHHCCHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHH
RRPLMTTDPIVGTGVNTISTGTPFTNPALFQPFDPLEILGVEPKVLLSSLASHRIIHAVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
ARLGWALDQSQASAALEWVKAEAFSRNVAIIEDEDFEHFLKGANFAQTSAAAKDHLRISR
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]