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The map label for this gene is rob [H]

Identifier: 22127597

GI number: 22127597

Start: 4137895

End: 4138761

Strand: Direct

Name: rob [H]

Synonym: y3723

Alternate gene names: 22127597

Gene position: 4137895-4138761 (Clockwise)

Preceding gene: 22127595

Following gene: 22127599

Centisome position: 89.94

GC content: 46.94

Gene sequence:

>867_bases
ATGGATCAAGCCAGTATCATTCGTGACTTGCTTAGCTGGCTAGAAAGTCATTTAGACCAGCCTTTAGCTCTGGACAATGT
TGCTGCCAAAGCGGGTTATTCGAAATGGCATTTGCAGCGAATGTTCAAGGATGTCACCGGGAATGCTATCGGTGCTTATA
TCCGTGCAAGAAGGCTATCAAAAGCCGCTGTCGCGTTACGTTTAACCAGTCGCCCTATTCTGGATATCGCTCTGCAATAT
CGTTTTGATTCGCAGCAAACTTTTACCCGCGCGTTTAAAAAACAGTTTGCACAGACGCCCGCATTATACCGACGGGCTGA
AGACTGGCACTCATCTGGAATATGTCCACCGATCCGCTTAGGAACATATACCCTGCCTCAACCTGAATTTATTACCTTAC
CCGAACAACATTTGGTAGGGATAACCCAAAGTTATTCTTGTACGCTTGAACAAATTTCAACACACCGTGCTGAATTACGC
TTACATTTTTGGCAACAATACCTCGGCGATGCCGATCAATTACCCCCAGTTCTGTATGGATTACATCACTCCCGGCCAAA
TCCGGAGAAAGATGACGAGCAAGAAATTTTCTATACGACGGCAATTGAACCACAGCATATTCCGTGTAACGTACCCGAGG
GCCAACCGGTTATCTTGCAGGGAGGTGAGTATGTGCAGTTCAGCTATGATGGGCCGCTTGATGGGTTACAAAATTTCATA
CTGACACTGTATGGCACCATTCTGCCTCAGTTGGCTCTGATCCGCAGACGTGGCTATGATATTGAACGTTTCTACCCGCA
GGGGAGGCCGAAAGATGGGCCTCCTGCTACGCTGAAATGTGACTATTTCATTCCAATTCGCCGTTAA

Upstream 100 bases:

>100_bases
CTTAAGCAGTCGGGAACATAAAAAGTTGCTACTATGCGCCAGAGCTATTCTGTTCATATTACTAATACCCTAAATTTAAG
CTGTTTTACGAGGAAGTTTT

Downstream 100 bases:

>100_bases
CGCTGTAATTCATCAAGTGCTGGCATATCTAAATGAGCGGTATCGCCAGCACTTTCAATCACCCAACCTGACGCCAACCA
CGGGCTTTCCTGATAATCAA

Product: right origin-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MDQASIIRDLLSWLESHLDQPLALDNVAAKAGYSKWHLQRMFKDVTGNAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRAEDWHSSGICPPIRLGTYTLPQPEFITLPEQHLVGITQSYSCTLEQISTHRAELR
LHFWQQYLGDADQLPPVLYGLHHSRPNPEKDDEQEIFYTTAIEPQHIPCNVPEGQPVILQGGEYVQFSYDGPLDGLQNFI
LTLYGTILPQLALIRRRGYDIERFYPQGRPKDGPPATLKCDYFIPIRR

Sequences:

>Translated_288_residues
MDQASIIRDLLSWLESHLDQPLALDNVAAKAGYSKWHLQRMFKDVTGNAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRAEDWHSSGICPPIRLGTYTLPQPEFITLPEQHLVGITQSYSCTLEQISTHRAELR
LHFWQQYLGDADQLPPVLYGLHHSRPNPEKDDEQEIFYTTAIEPQHIPCNVPEGQPVILQGGEYVQFSYDGPLDGLQNFI
LTLYGTILPQLALIRRRGYDIERFYPQGRPKDGPPATLKCDYFIPIRR
>Mature_288_residues
MDQASIIRDLLSWLESHLDQPLALDNVAAKAGYSKWHLQRMFKDVTGNAIGAYIRARRLSKAAVALRLTSRPILDIALQY
RFDSQQTFTRAFKKQFAQTPALYRRAEDWHSSGICPPIRLGTYTLPQPEFITLPEQHLVGITQSYSCTLEQISTHRAELR
LHFWQQYLGDADQLPPVLYGLHHSRPNPEKDDEQEIFYTTAIEPQHIPCNVPEGQPVILQGGEYVQFSYDGPLDGLQNFI
LTLYGTILPQLALIRRRGYDIERFYPQGRPKDGPPATLKCDYFIPIRR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=289, Percent_Identity=68.1660899653979, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=56.7307692307692, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=48.5148514851485, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33065; Mature: 33065

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQASIIRDLLSWLESHLDQPLALDNVAAKAGYSKWHLQRMFKDVTGNAIGAYIRARRLS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
KAAVALRLTSRPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRAEDWHSSGICPPIRL
HHHHEEEECCCCCEEHHEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
GTYTLPQPEFITLPEQHLVGITQSYSCTLEQISTHRAELRLHFWQQYLGDADQLPPVLYG
CEEECCCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LHHSRPNPEKDDEQEIFYTTAIEPQHIPCNVPEGQPVILQGGEYVQFSYDGPLDGLQNFI
HHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHH
LTLYGTILPQLALIRRRGYDIERFYPQGRPKDGPPATLKCDYFIPIRR
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MDQASIIRDLLSWLESHLDQPLALDNVAAKAGYSKWHLQRMFKDVTGNAIGAYIRARRLS
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
KAAVALRLTSRPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRAEDWHSSGICPPIRL
HHHHEEEECCCCCEEHHEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
GTYTLPQPEFITLPEQHLVGITQSYSCTLEQISTHRAELRLHFWQQYLGDADQLPPVLYG
CEEECCCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LHHSRPNPEKDDEQEIFYTTAIEPQHIPCNVPEGQPVILQGGEYVQFSYDGPLDGLQNFI
HHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHH
LTLYGTILPQLALIRRRGYDIERFYPQGRPKDGPPATLKCDYFIPIRR
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]