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The map label for this gene is leuD [H]

Identifier: 22127522

GI number: 22127522

Start: 4046327

End: 4046929

Strand: Direct

Name: leuD [H]

Synonym: y3648

Alternate gene names: 22127522

Gene position: 4046327-4046929 (Clockwise)

Preceding gene: 22127521

Following gene: 22127524

Centisome position: 87.95

GC content: 44.28

Gene sequence:

>603_bases
ATGGCAAAATTTATTCAACACATTGGTTTGGTTGCGCCGTTAGATGCAGCAAACGTCGATACCGACGCGATTATCCCTAA
GCAGTTTTTGCAAAAAGTGACTCGCACGGGCTTTGGTCAACACCTGTTTAACGATTGGCGTTTTTTGGATGATGCCGGCA
AGGTACCGAATCCTGATTTTGTCCTGAACCTGCCTCGCTATCAGGGTGCCACTATTTTGTTAGCCCGTGAGAATTTTGGC
TGTGGCTCCTCACGTGAACACGCACCATGGGCGCTGACGGATTTTGGTTTTAAAGTGGTTATCGCCCCGAGTTTTGCTGA
TATTTTTTATGGTAACGCGTTTAATAACCAATTATTACCGGTGACATTAAGCGAAGCGGATATTGATACCTTATTCCAAT
TGGTTAAAGAGAATGAAGGTATTGAGTTTGTGGTTGATCTCGAGCAGCAAACGGTTAATGCCGGTGGCAAAAGCTATGCT
TTCGAAATCGATCCTTTCCGCCGCCACTGTATGATTAATGGGTTGGATAGCATTGGCTTGACGTTGCAGCATGAACACAA
TATCTCAGCATACGAAAAACAACAGCCTGAATTTCTACGTTAA

Upstream 100 bases:

>100_bases
ATTTGGTCAGCCCAGCCATGGCCGCCGCTGCCGCCGTCAGTGGTCATTTTGCTGATGTTCGCGAATTATCCGCCACCACC
CACTAACCGGAGACACCACC

Downstream 100 bases:

>100_bases
ATAACCGTTAAGGCCAGCAGAACTAACATGCCAGCCTGTTTAGTAAATATATCGTTATTTTTAGTAGTAATAGATTGTGA
GTCATTAATAGATTGTAAGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MAKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDFVLNLPRYQGATILLARENFG
CGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLPVTLSEADIDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYA
FEIDPFRRHCMINGLDSIGLTLQHEHNISAYEKQQPEFLR

Sequences:

>Translated_200_residues
MAKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDFVLNLPRYQGATILLARENFG
CGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLPVTLSEADIDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYA
FEIDPFRRHCMINGLDSIGLTLQHEHNISAYEKQQPEFLR
>Mature_199_residues
AKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDFVLNLPRYQGATILLARENFGC
GSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLPVTLSEADIDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYAF
EIDPFRRHCMINGLDSIGLTLQHEHNISAYEKQQPEFLR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=79.6954314720812, Blast_Score=334, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321429, Length=203, Percent_Identity=53.2019704433498, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22472; Mature: 22340

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDF
CHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCE
VLNLPRYQGATILLARENFGCGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLP
EEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCEEE
VTLSEADIDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYAFEIDPFRRHCMINGLDSIGL
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCE
TLQHEHNISAYEKQQPEFLR
EEEECCCCCHHHCCCCCCCC
>Mature Secondary Structure 
AKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDF
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCE
VLNLPRYQGATILLARENFGCGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLP
EEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCEEE
VTLSEADIDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYAFEIDPFRRHCMINGLDSIGL
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCE
TLQHEHNISAYEKQQPEFLR
EEEECCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA