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The map label for this gene is cysM2 [H]

Identifier: 221230766

GI number: 221230766

Start: 2865834

End: 2867228

Strand: Reverse

Name: cysM2 [H]

Synonym: MLBr_02396

Alternate gene names: 221230766

Gene position: 2867228-2865834 (Counterclockwise)

Preceding gene: 221230767

Following gene: 221230765

Centisome position: 87.73

GC content: 62.72

Gene sequence:

>1395_bases
ATGCGGATTGCGCAGCACATCAGTGACCTCATCGGCGGCACGCCACTGGTCCGCCTGAATTCTGTCGTCCCGGCCGGTAC
CGGGGTCGTAGCGGCAAAGGTCGAATATTTCAACTCCGGGGGCAGCTCCAAGGACCGGATCGCGGCTAAAATGATCGATG
CGGCAGAGGCCAGCGGTCAGCTGAAGCCGGGCGGCACCATTGTCGAACCTACGTCGGGTAATACCGGTGTTGGTCTGGCT
TTAGTCGCCCAGCATCGCGGGTATAAGTGTGTGTTCGTCTGCCCCGACAAAGTTAGCGAGGACAAACGGAATGTGCTGTT
GGCCTACGGAGCCGAGGTAGTCGTATGTCCGACGGCCGTGCCGCCCGACGATCCGGACAGCTACTACAGCGTTTCCGACC
GGTTGGTCGCCAAGATCGACGGTGCCTGGAAACCAGACCAGTACGCCAACCCAGCAGGACCGGCCAGCCATTACGAGACC
ACTGGCCCGGAGATTTGGGCCGACACCGACGGTAGGGTAACCCATTTCGTCGCTGGCATCGGTACCGGTGGGACAATCAC
CGGCGTGGGCCGCTACCTCAAGGAGGTGTCCGGCGGATTGGTACGTATCATCGGTGCCGATCCGGAAGGGTCAGTGTATT
CGGGCGGTACCGGACGGCCATATCTGGTCGAGGGGGTGGGCGAGGACTTCTGGCCGGCGGCGTACGACCCGATGGTGCCC
GATCAGATCATCGCGGTGTCTGACTCCGACTCGTTCAATATGACCAGGCGGTTGGCCCGCGAAGAAGCGATGCTGGTCGG
CGGGTCGTGTGGCATGGCGGTCGTGGCTGCTCTGCAAGTGGCCGAAGAAGCCGGGCCGGCCGCGCTGGTCGTGGTCTTGC
TGCCGGACGGCGGCCGTGGCTACATGTCCAAGATCTTCAATGACGCCTGGATGTCGTCGTATGGGTTCCTGCGTAGCCGT
CTGGACGGGTCTACCGAGCAGCCCATAGTGGGTGATGTGTTGCGTCGGAAATCGGGCGCGTTGCCCGACTTGGTGCACAC
CCATCCGTCGGAGACGGTGCGCGATGCCATTGGCATTCTGCGTGAGTACGCGGTATCCCAGATCCCGGTTGTCGGCGCCG
AGCCTCCGGTGATGGCGGGCGAGGTTGTCGGCAGCGTCTCCGAACGGGAACTACTTTCGGCCGTGTTTGAAGGTCGTGCC
AGCTTGGCCGACGCCGTCTCGGAGCACATGGGCCCGCCACTACGACTAATCGGCGACGGTGAGTTGGTTAGTGTGGCCGG
TGAGGCGCTGCGCGACTCCGATGCGCTGATGGTGGTGGAGAAAGGCAAACTGGTCGGGGTCATAACGCGATACGACTTGT
TGGGTTTCCTCTCGGAGGGCGCACCGCTACGTTAG

Upstream 100 bases:

>100_bases
TGGCGAGTACATCCTCGAGGCCACCGGATAGCGGATCGGGGTGGTTCGCCATCACCCGAGTGGTTGTGGGTGGCAAGAAT
TCGCCTGAGATTATGAACAC

Downstream 100 bases:

>100_bases
CGGGATGATCGAGCCGCAAGTTAGTTCGGCTACAAACCAAACACGGCGACGACTCGATACGCCGAAACCCGTGCCCGTTC
GCTACGCGAATTTGTTCTCA

Product: cystathionine beta-synthase

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRIAQHISDLIGGTPLVRLNSVVPAGTGVVAAKVEYFNSGGSSKDRIAAKMIDAAEASGQLKPGGTIVEPTSGNTGVGLA
LVAQHRGYKCVFVCPDKVSEDKRNVLLAYGAEVVVCPTAVPPDDPDSYYSVSDRLVAKIDGAWKPDQYANPAGPASHYET
TGPEIWADTDGRVTHFVAGIGTGGTITGVGRYLKEVSGGLVRIIGADPEGSVYSGGTGRPYLVEGVGEDFWPAAYDPMVP
DQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVVAALQVAEEAGPAALVVVLLPDGGRGYMSKIFNDAWMSSYGFLRSR
LDGSTEQPIVGDVLRRKSGALPDLVHTHPSETVRDAIGILREYAVSQIPVVGAEPPVMAGEVVGSVSERELLSAVFEGRA
SLADAVSEHMGPPLRLIGDGELVSVAGEALRDSDALMVVEKGKLVGVITRYDLLGFLSEGAPLR

Sequences:

>Translated_464_residues
MRIAQHISDLIGGTPLVRLNSVVPAGTGVVAAKVEYFNSGGSSKDRIAAKMIDAAEASGQLKPGGTIVEPTSGNTGVGLA
LVAQHRGYKCVFVCPDKVSEDKRNVLLAYGAEVVVCPTAVPPDDPDSYYSVSDRLVAKIDGAWKPDQYANPAGPASHYET
TGPEIWADTDGRVTHFVAGIGTGGTITGVGRYLKEVSGGLVRIIGADPEGSVYSGGTGRPYLVEGVGEDFWPAAYDPMVP
DQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVVAALQVAEEAGPAALVVVLLPDGGRGYMSKIFNDAWMSSYGFLRSR
LDGSTEQPIVGDVLRRKSGALPDLVHTHPSETVRDAIGILREYAVSQIPVVGAEPPVMAGEVVGSVSERELLSAVFEGRA
SLADAVSEHMGPPLRLIGDGELVSVAGEALRDSDALMVVEKGKLVGVITRYDLLGFLSEGAPLR
>Mature_464_residues
MRIAQHISDLIGGTPLVRLNSVVPAGTGVVAAKVEYFNSGGSSKDRIAAKMIDAAEASGQLKPGGTIVEPTSGNTGVGLA
LVAQHRGYKCVFVCPDKVSEDKRNVLLAYGAEVVVCPTAVPPDDPDSYYSVSDRLVAKIDGAWKPDQYANPAGPASHYET
TGPEIWADTDGRVTHFVAGIGTGGTITGVGRYLKEVSGGLVRIIGADPEGSVYSGGTGRPYLVEGVGEDFWPAAYDPMVP
DQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVVAALQVAEEAGPAALVVVLLPDGGRGYMSKIFNDAWMSSYGFLRSR
LDGSTEQPIVGDVLRRKSGALPDLVHTHPSETVRDAIGILREYAVSQIPVVGAEPPVMAGEVVGSVSERELLSAVFEGRA
SLADAVSEHMGPPLRLIGDGELVSVAGEALRDSDALMVVEKGKLVGVITRYDLLGFLSEGAPLR

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=478, Percent_Identity=39.3305439330544, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI295821200, Length=478, Percent_Identity=39.3305439330544, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI4557415, Length=478, Percent_Identity=39.3305439330544, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI2367138, Length=310, Percent_Identity=38.3870967741935, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1788754, Length=331, Percent_Identity=39.5770392749245, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17534315, Length=325, Percent_Identity=44.3076923076923, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25147552, Length=323, Percent_Identity=42.7244582043344, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17562970, Length=310, Percent_Identity=40, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566674, Length=281, Percent_Identity=42.7046263345196, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI115535073, Length=331, Percent_Identity=41.0876132930514, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=39.5973154362416, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17561720, Length=328, Percent_Identity=38.4146341463415, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32566672, Length=217, Percent_Identity=37.3271889400922, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321594, Length=505, Percent_Identity=32.8712871287129, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321449, Length=333, Percent_Identity=34.8348348348348, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24643623, Length=483, Percent_Identity=34.5755693581781, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI20129101, Length=483, Percent_Identity=34.5755693581781, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 48563; Mature: 48563

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAQHISDLIGGTPLVRLNSVVPAGTGVVAAKVEYFNSGGSSKDRIAAKMIDAAEASGQ
CCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
LKPGGTIVEPTSGNTGVGLALVAQHRGYKCVFVCPDKVSEDKRNVLLAYGAEVVVCPTAV
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCC
PPDDPDSYYSVSDRLVAKIDGAWKPDQYANPAGPASHYETTGPEIWADTDGRVTHFVAGI
CCCCCCCHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEC
GTGGTITGVGRYLKEVSGGLVRIIGADPEGSVYSGGTGRPYLVEGVGEDFWPAAYDPMVP
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCC
DQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVVAALQVAEEAGPAALVVVLLPDGGRG
CCEEEECCCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCH
YMSKIFNDAWMSSYGFLRSRLDGSTEQPIVGDVLRRKSGALPDLVHTHPSETVRDAIGIL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHH
REYAVSQIPVVGAEPPVMAGEVVGSVSERELLSAVFEGRASLADAVSEHMGPPLRLIGDG
HHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
ELVSVAGEALRDSDALMVVEKGKLVGVITRYDLLGFLSEGAPLR
CEEEHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRIAQHISDLIGGTPLVRLNSVVPAGTGVVAAKVEYFNSGGSSKDRIAAKMIDAAEASGQ
CCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
LKPGGTIVEPTSGNTGVGLALVAQHRGYKCVFVCPDKVSEDKRNVLLAYGAEVVVCPTAV
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCEEEEECCCC
PPDDPDSYYSVSDRLVAKIDGAWKPDQYANPAGPASHYETTGPEIWADTDGRVTHFVAGI
CCCCCCCHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEC
GTGGTITGVGRYLKEVSGGLVRIIGADPEGSVYSGGTGRPYLVEGVGEDFWPAAYDPMVP
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCC
DQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVVAALQVAEEAGPAALVVVLLPDGGRG
CCEEEECCCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCH
YMSKIFNDAWMSSYGFLRSRLDGSTEQPIVGDVLRRKSGALPDLVHTHPSETVRDAIGIL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHH
REYAVSQIPVVGAEPPVMAGEVVGSVSERELLSAVFEGRASLADAVSEHMGPPLRLIGDG
HHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
ELVSVAGEALRDSDALMVVEKGKLVGVITRYDLLGFLSEGAPLR
CEEEHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]