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The map label for this gene is pafB

Identifier: 221230167

GI number: 221230167

Start: 1581189

End: 1582184

Strand: Direct

Name: pafB

Synonym: MLBr_01329

Alternate gene names: 221230167

Gene position: 1581189-1582184 (Clockwise)

Preceding gene: 221230166

Following gene: 221230168

Centisome position: 48.38

GC content: 62.95

Gene sequence:

>996_bases
ATGGCGACGTCGAAAGTTGAACGGCTGGTCAACCTGGTTATCGCCTTGCTGTCGACCCGTGGCTACATGACCGCTGAAAA
GATCAGATCCAGCGTGGCGGGTTACTCCGACAGTCCTACCGTAGAGGCGTTCTCTCGGATGTTTGAGCGCGATAAGAACG
AGTTACGTGACCTTGGCATACCGCTCGAGGTCGGCAAGGTGTCGGCGCTAGACCCCTCCGAGGGCTACCGCATCAACCGC
GATGCGTACGCACTTCCTCCCGTCGAGCTAACCCCGGACGAAGCGGCTGCAGTAGCTGTCGCAACTCAGCTGTGGGAATC
GCAAGAACTGATCACCGCGACACAAGGCGCATTGCTCAAGCTGCGGGCCGCCGGGGTTGACATCGACCCCCTCGATACAC
CGGTGGTCATCGCCTCATCGTCTGGGGTGTCAAGTCTGCGTGGGTCGGAGGATTTTCTATCAATCCTGTTGTCGGCCATT
GGTTCTCGGCAGGCAGTGCAGTTTCCGTACCGGCCGTCACGGGCTGAGCCTTACACCATGCGCAACGTCGAACCGTGGGG
TGTGATCACCGAGAACAGTTGCTGGTATCTCGTTGGCCACGACTGCGATCGCAACGCCACCCGCACTTTCCGGCTCTCTC
GGATCGGTTCGGAGGTCGCGCCGATCGGGCCTGCCGGCGCTGTCACCGTGCCTGACGGCGTCGATTTGCGCAGAATCGTG
TCTGACGCCGTCGCCGAAGTGTCGACCGGCGCTACCGCCCGGGTGTGGGTCGTCGACGGACGGGCCACTGCGTTGCGGCA
CGCCGGACGACCCGCCGGTGTTCGGCGACTGGGCGGCCGCGACGGTCAGGTGATCGAACTCGACATTGGATCCATCGACC
GGCTGGCCCGTGACATCGCTGGCCACGGTGCTGACGCTGTCGTGCTCGAGCCAGACGCTCTGCGCGACGATGTGTTGATA
CGGTTGCGCGCCCACGCGGGAACAGGGCCTTCATGA

Upstream 100 bases:

>100_bases
TAAACTTAACGACCAAGCGCAGCGCACGGTGTTGTGCAAAGACCCCTTCCGCGCGGTCGACGAGCGGGTCAAGCGGCTGA
TCGCGAGCATGTAGCTATAC

Downstream 100 bases:

>100_bases
CACAGCTGTCCACCCGCCTAGTGCGGCTGCTCAATATGGTGCCGTACTTCCAGGCTAACCCGCGGATTACCCGTGCGGAA
GCTGCCGCCGATCTGGGAGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MATSKVERLVNLVIALLSTRGYMTAEKIRSSVAGYSDSPTVEAFSRMFERDKNELRDLGIPLEVGKVSALDPSEGYRINR
DAYALPPVELTPDEAAAVAVATQLWESQELITATQGALLKLRAAGVDIDPLDTPVVIASSSGVSSLRGSEDFLSILLSAI
GSRQAVQFPYRPSRAEPYTMRNVEPWGVITENSCWYLVGHDCDRNATRTFRLSRIGSEVAPIGPAGAVTVPDGVDLRRIV
SDAVAEVSTGATARVWVVDGRATALRHAGRPAGVRRLGGRDGQVIELDIGSIDRLARDIAGHGADAVVLEPDALRDDVLI
RLRAHAGTGPS

Sequences:

>Translated_331_residues
MATSKVERLVNLVIALLSTRGYMTAEKIRSSVAGYSDSPTVEAFSRMFERDKNELRDLGIPLEVGKVSALDPSEGYRINR
DAYALPPVELTPDEAAAVAVATQLWESQELITATQGALLKLRAAGVDIDPLDTPVVIASSSGVSSLRGSEDFLSILLSAI
GSRQAVQFPYRPSRAEPYTMRNVEPWGVITENSCWYLVGHDCDRNATRTFRLSRIGSEVAPIGPAGAVTVPDGVDLRRIV
SDAVAEVSTGATARVWVVDGRATALRHAGRPAGVRRLGGRDGQVIELDIGSIDRLARDIAGHGADAVVLEPDALRDDVLI
RLRAHAGTGPS
>Mature_330_residues
ATSKVERLVNLVIALLSTRGYMTAEKIRSSVAGYSDSPTVEAFSRMFERDKNELRDLGIPLEVGKVSALDPSEGYRINRD
AYALPPVELTPDEAAAVAVATQLWESQELITATQGALLKLRAAGVDIDPLDTPVVIASSSGVSSLRGSEDFLSILLSAIG
SRQAVQFPYRPSRAEPYTMRNVEPWGVITENSCWYLVGHDCDRNATRTFRLSRIGSEVAPIGPAGAVTVPDGVDLRRIVS
DAVAEVSTGATARVWVVDGRATALRHAGRPAGVRRLGGRDGQVIELDIGSIDRLARDIAGHGADAVVLEPDALRDDVLIR
LRAHAGTGPS

Specific function: Does not seem to be involved in pupylation or substrate degradation

COG id: COG2378

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pafB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAFB_MYCLE (P54076)

Other databases:

- EMBL:   U00017
- EMBL:   AL035310
- EMBL:   AL583921
- PIR:   S72868
- RefSeq:   NP_301953.1
- EnsemblBacteria:   EBMYCT00000027905
- GeneID:   910438
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1329
- NMPDR:   fig|272631.1.peg.825
- Leproma:   ML1329
- GeneTree:   EBGT00050000016808
- HOGENOM:   HBG481015
- OMA:   MFERDKD
- ProtClustDB:   CLSK791626
- BioCyc:   MLEP272631:ML1329-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35404; Mature: 35273

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSKVERLVNLVIALLSTRGYMTAEKIRSSVAGYSDSPTVEAFSRMFERDKNELRDLGI
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PLEVGKVSALDPSEGYRINRDAYALPPVELTPDEAAAVAVATQLWESQELITATQGALLK
CEECCCEECCCCCCCEEECCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEE
LRAAGVDIDPLDTPVVIASSSGVSSLRGSEDFLSILLSAIGSRQAVQFPYRPSRAEPYTM
EEECCCCCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEE
RNVEPWGVITENSCWYLVGHDCDRNATRTFRLSRIGSEVAPIGPAGAVTVPDGVDLRRIV
CCCCCCCEEECCCEEEEECCCCCCCCHHEEEHHHHCCCCCCCCCCCCEECCCCCHHHHHH
SDAVAEVSTGATARVWVVDGRATALRHAGRPAGVRRLGGRDGQVIELDIGSIDRLARDIA
HHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHH
GHGADAVVLEPDALRDDVLIRLRAHAGTGPS
CCCCCEEEECCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
ATSKVERLVNLVIALLSTRGYMTAEKIRSSVAGYSDSPTVEAFSRMFERDKNELRDLGI
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PLEVGKVSALDPSEGYRINRDAYALPPVELTPDEAAAVAVATQLWESQELITATQGALLK
CEECCCEECCCCCCCEEECCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEE
LRAAGVDIDPLDTPVVIASSSGVSSLRGSEDFLSILLSAIGSRQAVQFPYRPSRAEPYTM
EEECCCCCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEE
RNVEPWGVITENSCWYLVGHDCDRNATRTFRLSRIGSEVAPIGPAGAVTVPDGVDLRRIV
CCCCCCCEEECCCEEEEECCCCCCCCHHEEEHHHHCCCCCCCCCCCCEECCCCCHHHHHH
SDAVAEVSTGATARVWVVDGRATALRHAGRPAGVRRLGGRDGQVIELDIGSIDRLARDIA
HHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHHH
GHGADAVVLEPDALRDDVLIRLRAHAGTGPS
CCCCCEEEECCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002