Click here to switch to the map view.

The map label for this gene is pafA

Identifier: 221230166

GI number: 221230166

Start: 1579824

End: 1581182

Strand: Direct

Name: pafA

Synonym: MLBr_01328

Alternate gene names: 221230166

Gene position: 1579824-1581182 (Clockwise)

Preceding gene: 221230165

Following gene: 221230167

Centisome position: 48.34

GC content: 60.04

Gene sequence:

>1359_bases
GTGCAGCGTCGAATTATGGGAATCGAAACCGAGTTCGGTGTCACCTGCACATTTCATGGTCACCGTCGTCTTTCCCCGGA
TGAGGTGGCCCGCTATCTGTTCCGTCGAGTGGTGTCCTGGGGCCGCAGCTCCAATGTCTTCCTGCGTAACGGTGCCCGCC
TCTATCTTGACGTGGGCAGTCATCCCGAGTATGCTACCGCCGAATGCGACAACCTGGTGCAGCTTGTCACCCATGACCGG
GCTGGCGAATGGGTGCTAGAAGACCTGCTCGTTGATGCCGAGCAGCGGCTCGCCGACGAAGGCATCGGTGGCGACATTTA
CCTGTTCAAGAACAACACCGACTCGGCGGGAAACTCCTACGGCTGTCACGAAAACTACCTGATCGTGCGCGCTGGTGAGT
TCTCCAGAATTTCGGATGTGCTGCTGCCGTTCCTGGTCACTCGTCAGTTGATCTGCGGAGCCGGCAAGGTGCTGCAAACT
CCCAAAGCCGCGACGTTCTGCCTGTCACAACGTGCTGAGCATATCTGGGAGGGCGTCTCTTCGGCGACTACCCGTAGCCG
ACCGATTATCAACACCCGCGACGAGCCACACGCCGACGCTGAGAAGTATAGGCGGCTGCACGTCATCGTCGGTGACTCCA
ATATGTGCGAGACCACCACCATGCTCAAGGTGGGCACCGCCGCGCTAATGCTGGAAATGGTCGAAACCGGGGTTCCATTT
CGCGATTTTTCTCTGGACAACCCTATCCGCGCCATCCGGGAGGTCAGCCATGACATCACTGGGCGCCGTCCGGTGCGGTT
GGCTGGCGGGCGTCAAGCTAGTGCGCTGGACATCCAACGGGAATATTACACCCGGGCCTTCGAACATCTGCAAACCCGAG
AGCCTAACGTGCAGTTCGAGCAGGTTGTTGACTTGTGGGGCCGTCAGCTCGACGCCATTGAGAGCCAGGATTTCGCCAAG
GTGGATACCGAGATCGACTGGGTGATCAAACGCAAGCTGTTTCAGCGCTACCAGGACCGCGACAACATGGAGCTGACCGA
TCCGAAGATCGCCCAGCTGGACCTGGCCTATCACGACATCAAGCGTGGGCGCGGTGTCTTCGATCTGCTGCAGCGTAAGG
GGCTGGCGGCGCGTGTCACGACCGACGAAGACATTGCCGACGCGGTCAACCACCCGCCGCAAACCACCCGGGCTAGGCTG
CGCGGCGAGTTCATCAGCGCCGCGCAGGCGGCGGGACGTGATTTCACTGTCGACTGGGTACATCTTAAACTTAACGACCA
AGCGCAGCGCACGGTGTTGTGCAAAGACCCCTTCCGCGCGGTCGACGAGCGGGTCAAGCGGCTGATCGCGAGCATGTAG

Upstream 100 bases:

>100_bases
TTTTGTTGCTAGGGGGGTGGCACTGACATTGATGGTTGGTTGGTACCCGTGGGCGTGGCTCGCCCACCGCGATGCTCAAA
CAACCAGTACCCTCAAAGAT

Downstream 100 bases:

>100_bases
CTATACATGGCGACGTCGAAAGTTGAACGGCTGGTCAACCTGGTTATCGCCTTGCTGTCGACCCGTGGCTACATGACCGC
TGAAAAGATCAGATCCAGCG

Product: putative DNA-binding protein

Products: NA

Alternate protein names: Proteasome accessory factor A; Pup-conjugating enzyme

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDNLVQLVTHDR
AGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
PKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCETTTMLKVGTAALMLEMVETGVPF
RDFSLDNPIRAIREVSHDITGRRPVRLAGGRQASALDIQREYYTRAFEHLQTREPNVQFEQVVDLWGRQLDAIESQDFAK
VDTEIDWVIKRKLFQRYQDRDNMELTDPKIAQLDLAYHDIKRGRGVFDLLQRKGLAARVTTDEDIADAVNHPPQTTRARL
RGEFISAAQAAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM

Sequences:

>Translated_452_residues
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDNLVQLVTHDR
AGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
PKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCETTTMLKVGTAALMLEMVETGVPF
RDFSLDNPIRAIREVSHDITGRRPVRLAGGRQASALDIQREYYTRAFEHLQTREPNVQFEQVVDLWGRQLDAIESQDFAK
VDTEIDWVIKRKLFQRYQDRDNMELTDPKIAQLDLAYHDIKRGRGVFDLLQRKGLAARVTTDEDIADAVNHPPQTTRARL
RGEFISAAQAAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM
>Mature_452_residues
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDNLVQLVTHDR
AGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
PKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCETTTMLKVGTAALMLEMVETGVPF
RDFSLDNPIRAIREVSHDITGRRPVRLAGGRQASALDIQREYYTRAFEHLQTREPNVQFEQVVDLWGRQLDAIESQDFAK
VDTEIDWVIKRKLFQRYQDRDNMELTDPKIAQLDLAYHDIKRGRGVFDLLQRKGLAARVTTDEDIADAVNHPPQTTRARL
RGEFISAAQAAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM

Specific function: Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAFA_MYCLB (B8ZRF5)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503582.1
- EnsemblBacteria:   EBMYCT00000085218
- GeneID:   7326473
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016626
- HOGENOM:   HBG292322
- OMA:   EYATPEC
- ProtClustDB:   CLSK872004
- HAMAP:   MF_02111
- InterPro:   IPR022279
- InterPro:   IPR004347
- PIRSF:   PIRSF018077
- TIGRFAMs:   TIGR03686

Pfam domain/function: PF03136 DUF245

EC number: NA

Molecular weight: Translated: 51459; Mature: 51459

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGS
CCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCC
HPEYATAECDNLVQLVTHDRAGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSY
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATFCLSQRAEHIWEGVS
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHH
SATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCETTTMLKVGTAALMLEMVETGVPF
HHHHCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
RDFSLDNPIRAIREVSHDITGRRPVRLAGGRQASALDIQREYYTRAFEHLQTREPNVQFE
CCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
QVVDLWGRQLDAIESQDFAKVDTEIDWVIKRKLFQRYQDRDNMELTDPKIAQLDLAYHDI
HHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KRGRGVFDLLQRKGLAARVTTDEDIADAVNHPPQTTRARLRGEFISAAQAAGRDFTVDWV
HHCCCHHHHHHCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
HLKLNDQAQRTVLCKDPFRAVDERVKRLIASM
EEEECCCCCEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGS
CCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCC
HPEYATAECDNLVQLVTHDRAGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSY
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATFCLSQRAEHIWEGVS
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHH
SATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCETTTMLKVGTAALMLEMVETGVPF
HHHHCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
RDFSLDNPIRAIREVSHDITGRRPVRLAGGRQASALDIQREYYTRAFEHLQTREPNVQFE
CCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
QVVDLWGRQLDAIESQDFAKVDTEIDWVIKRKLFQRYQDRDNMELTDPKIAQLDLAYHDI
HHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KRGRGVFDLLQRKGLAARVTTDEDIADAVNHPPQTTRARLRGEFISAAQAAGRDFTVDWV
HHCCCHHHHHHCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
HLKLNDQAQRTVLCKDPFRAVDERVKRLIASM
EEEECCCCCEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA