Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 221230125

GI number: 221230125

Start: 1507601

End: 1508386

Strand: Direct

Name: hisF

Synonym: MLBr_01263

Alternate gene names: 221230125

Gene position: 1507601-1508386 (Clockwise)

Preceding gene: 221230124

Following gene: 221230126

Centisome position: 46.13

GC content: 62.09

Gene sequence:

>786_bases
ATGTACTCGGGTAACGGTCTTGCGGTACGAGTGATTCCTTGCCTGGACGTCTATTGTGGGCGTGTGGTTAAAGGGGTCAA
TTTCAAGAACCTCCGGGATGCGGGTGATCTCGTGGAGTTGGCTGCCGCCTACGATGCTGAAGGCGCCGACGAGCTGGCTT
TTCTTGACGTGACTGCGTCGTCGTCGGGCAGGGCCACCATGCTGGAGGTGGTGCGCTGCACCGCCGAGCAGGTGTTCATC
CCGTTGATGGTCGGCGGTGGGGTGCGTACGGTCGCTGACGTCGACGTGTTGCTGCGTGCGGGAGCTGACAAGGTTGCGGT
CAACACCGCAGCAATTGCTCGTCCCGAGCTATTGGCCGACATGGCAGGGCAATTCGGTTCTCAATGCATCGTGTTATCTG
TCGACGCGCGCACGGTACCCACCGGATCAGCGCGAACACCGTCCGGGTGGGAGGCCACTACTCATGGCGGTTACCGCGGC
ACCGGTATTGACGCTGTTGAGTGGGCGGCCCGTGGGGCCGACCTTGGGGTGGGAGAAATCCTGCTGAACTCGATGGACGC
CGACGGCACTAAAGCCGGGTTCGACTTGGCGATGTTGCGGGCTGTTCGTGCCGCGGTCACGGTGCCGGTCATAGCTAGCG
GCGGCGCCGGGGCAATCGAACACTTCGTGCCAGCGGTTACTGCCGGTGCTGATGCGGTTTTGGCGGCCAGTGTCTTTCAT
TTCCGAGAGCTAACGATCGGGCAGGTGAAAGACGCTATGGCCGCAGCAGGGATCGCGGTGCGATGA

Upstream 100 bases:

>100_bases
TGGACAACCACGGCGTCGTGTGTGGTAAATGCGGCACCCGGCGTGCATAGCCAGATCTTCGAGATCCTTTGCGGCATGAG
CGAACCGGAGGATTACCAAG

Downstream 100 bases:

>100_bases
CACTCGACCCAGACATTGCTGTACGCCTCAAGCGCAACGCCGAAGGTTTATTCACCGCCGTCGTACAGGAGCGCTCCAGC
GGAGACGTGCTAATGGTCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MYSGNGLAVRVIPCLDVYCGRVVKGVNFKNLRDAGDLVELAAAYDAEGADELAFLDVTASSSGRATMLEVVRCTAEQVFI
PLMVGGGVRTVADVDVLLRAGADKVAVNTAAIARPELLADMAGQFGSQCIVLSVDARTVPTGSARTPSGWEATTHGGYRG
TGIDAVEWAARGADLGVGEILLNSMDADGTKAGFDLAMLRAVRAAVTVPVIASGGAGAIEHFVPAVTAGADAVLAASVFH
FRELTIGQVKDAMAAAGIAVR

Sequences:

>Translated_261_residues
MYSGNGLAVRVIPCLDVYCGRVVKGVNFKNLRDAGDLVELAAAYDAEGADELAFLDVTASSSGRATMLEVVRCTAEQVFI
PLMVGGGVRTVADVDVLLRAGADKVAVNTAAIARPELLADMAGQFGSQCIVLSVDARTVPTGSARTPSGWEATTHGGYRG
TGIDAVEWAARGADLGVGEILLNSMDADGTKAGFDLAMLRAVRAAVTVPVIASGGAGAIEHFVPAVTAGADAVLAASVFH
FRELTIGQVKDAMAAAGIAVR
>Mature_261_residues
MYSGNGLAVRVIPCLDVYCGRVVKGVNFKNLRDAGDLVELAAAYDAEGADELAFLDVTASSSGRATMLEVVRCTAEQVFI
PLMVGGGVRTVADVDVLLRAGADKVAVNTAAIARPELLADMAGQFGSQCIVLSVDARTVPTGSARTPSGWEATTHGGYRG
TGIDAVEWAARGADLGVGEILLNSMDADGTKAGFDLAMLRAVRAAVTVPVIASGGAGAIEHFVPAVTAGADAVLAASVFH
FRELTIGQVKDAMAAAGIAVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=44.4015444015444, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=28.3720930232558, Blast_Score=78, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=34.2767295597484, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_MYCLB (B8ZRB4)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503541.1
- ProteinModelPortal:   B8ZRB4
- SMR:   B8ZRB4
- EnsemblBacteria:   EBMYCT00000085297
- GeneID:   7324716
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015857
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26664; Mature: 26664

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 16-16 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSGNGLAVRVIPCLDVYCGRVVKGVNFKNLRDAGDLVELAAAYDAEGADELAFLDVTAS
CCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
SSGRATMLEVVRCTAEQVFIPLMVGGGVRTVADVDVLLRAGADKVAVNTAAIARPELLAD
CCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHCCHHHHHH
MAGQFGSQCIVLSVDARTVPTGSARTPSGWEATTHGGYRGTGIDAVEWAARGADLGVGEI
HHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
LLNSMDADGTKAGFDLAMLRAVRAAVTVPVIASGGAGAIEHFVPAVTAGADAVLAASVFH
HHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
FRELTIGQVKDAMAAAGIAVR
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYSGNGLAVRVIPCLDVYCGRVVKGVNFKNLRDAGDLVELAAAYDAEGADELAFLDVTAS
CCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECC
SSGRATMLEVVRCTAEQVFIPLMVGGGVRTVADVDVLLRAGADKVAVNTAAIARPELLAD
CCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHCCHHHHHH
MAGQFGSQCIVLSVDARTVPTGSARTPSGWEATTHGGYRGTGIDAVEWAARGADLGVGEI
HHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHH
LLNSMDADGTKAGFDLAMLRAVRAAVTVPVIASGGAGAIEHFVPAVTAGADAVLAASVFH
HHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
FRELTIGQVKDAMAAAGIAVR
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA