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The map label for this gene is hisH

Identifier: 221230123

GI number: 221230123

Start: 1505426

End: 1506046

Strand: Direct

Name: hisH

Synonym: MLBr_01260

Alternate gene names: 221230123

Gene position: 1505426-1506046 (Clockwise)

Preceding gene: 221230122

Following gene: 221230124

Centisome position: 46.06

GC content: 60.23

Gene sequence:

>621_bases
GTGAGAAGTAAATCGGTTGTAGTTCTGGATTACGGTTCGGGTAATTTGTGGTCGGTGCAGCGCGCGCTGCAGCGGGTTGG
TGCCGCGGTCGAAGTTACGGCTGACTCAGCTGCAGGCGCCGCCGCTGACGGGTTGCTGGTGCCCGGTGTCGGCGCGTTCG
AGGCGTGCATGGCAGGTCTGCGGAAAATCGCGGGTGAACGGACCATCGCCGAGCGGATCGTCGCGGGACGGCCGGTATTA
GGGGTCTGTGTCGGCATGCAGATTTTGTTCGCCCGCGGTGTCGAATTCGGTGTGGAAACAACAGGCTGTAGGCAGTGGCC
GGGCGTTGTGACCCGGCTCGACGCCCCGGTGGTTCCCCATATGGGCTGGAACGTAGTCGATTCCGCTTCTGGAAGTGCGT
TGTTCAAGGGCCTAGATGCTGGAGTGCGGTTCTATTTCGTGCATTCTTATGCCGCGCAGCGTTGGGAAGGCTCATCCAAG
GCGCTGCTGACATGGGCAACTCACCAAGTACCGTTTTTGGCCGCGGTGGAAGAAGGACCACTGGTTGCCACCCAGTTCCA
TCCGGAGAAGAGTGGGGACGCTGGAGCAACCTTGCTGAGCAATTGGCTTGGGGAACTTTAA

Upstream 100 bases:

>100_bases
ACATCACTGAAGCGCAATACAAAGCCGTCGCGCGCGCGTTAAGTGAGGCGGTCAAATTCGACCCTCGGTTTTCGGGCGTG
CCGTCCACCAAAGGTGTCCT

Downstream 100 bases:

>100_bases
AGGATGCTGTTGATGTCGCTGATACTTTTACCGGCTGTCGACGTGGTCGAGGGGCGCGCCGTGCGCCTGGTTCAAGGAAA
GGCCGGTAGCGAAAACGATT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MRSKSVVVLDYGSGNLWSVQRALQRVGAAVEVTADSAAGAAADGLLVPGVGAFEACMAGLRKIAGERTIAERIVAGRPVL
GVCVGMQILFARGVEFGVETTGCRQWPGVVTRLDAPVVPHMGWNVVDSASGSALFKGLDAGVRFYFVHSYAAQRWEGSSK
ALLTWATHQVPFLAAVEEGPLVATQFHPEKSGDAGATLLSNWLGEL

Sequences:

>Translated_206_residues
MRSKSVVVLDYGSGNLWSVQRALQRVGAAVEVTADSAAGAAADGLLVPGVGAFEACMAGLRKIAGERTIAERIVAGRPVL
GVCVGMQILFARGVEFGVETTGCRQWPGVVTRLDAPVVPHMGWNVVDSASGSALFKGLDAGVRFYFVHSYAAQRWEGSSK
ALLTWATHQVPFLAAVEEGPLVATQFHPEKSGDAGATLLSNWLGEL
>Mature_206_residues
MRSKSVVVLDYGSGNLWSVQRALQRVGAAVEVTADSAAGAAADGLLVPGVGAFEACMAGLRKIAGERTIAERIVAGRPVL
GVCVGMQILFARGVEFGVETTGCRQWPGVVTRLDAPVVPHMGWNVVDSASGSALFKGLDAGVRFYFVHSYAAQRWEGSSK
ALLTWATHQVPFLAAVEEGPLVATQFHPEKSGDAGATLLSNWLGEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=205, Percent_Identity=41.4634146341463, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=211, Percent_Identity=36.0189573459716, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_MYCLE (Q9X7C0)

Other databases:

- EMBL:   AL049913
- EMBL:   AL583921
- PIR:   T45249
- RefSeq:   NP_301909.1
- ProteinModelPortal:   Q9X7C0
- SMR:   Q9X7C0
- EnsemblBacteria:   EBMYCT00000029487
- GeneID:   910371
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1260
- NMPDR:   fig|272631.1.peg.781
- Leproma:   ML1260
- GeneTree:   EBGT00050000016176
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13146
- BioCyc:   MLEP272631:ML1260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21652; Mature: 21652

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I ; PS00217 SUGAR_TRANSPORT_2

Important sites: ACT_SITE 83-83 ACT_SITE 187-187 ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKSVVVLDYGSGNLWSVQRALQRVGAAVEVTADSAAGAAADGLLVPGVGAFEACMAGL
CCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHH
RKIAGERTIAERIVAGRPVLGVCVGMQILFARGVEFGVETTGCRQWPGVVTRLDAPVVPH
HHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEECCCCCCCC
MGWNVVDSASGSALFKGLDAGVRFYFVHSYAAQRWEGSSKALLTWATHQVPFLAAVEEGP
CCCCEEECCCCHHHHHHHCCCEEEEEEEHHHHHHCCCCCCEEEEEECCCCCEEEEECCCC
LVATQFHPEKSGDAGATLLSNWLGEL
EEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRSKSVVVLDYGSGNLWSVQRALQRVGAAVEVTADSAAGAAADGLLVPGVGAFEACMAGL
CCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHH
RKIAGERTIAERIVAGRPVLGVCVGMQILFARGVEFGVETTGCRQWPGVVTRLDAPVVPH
HHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEECCCCCCCC
MGWNVVDSASGSALFKGLDAGVRFYFVHSYAAQRWEGSSKALLTWATHQVPFLAAVEEGP
CCCCEEECCCCHHHHHHHCCCEEEEEEEHHHHHHCCCCCCEEEEEECCCCCEEEEECCCC
LVATQFHPEKSGDAGATLLSNWLGEL
EEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002