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The map label for this gene is nadB [H]

Identifier: 221230102

GI number: 221230102

Start: 1447795

End: 1449378

Strand: Direct

Name: nadB [H]

Synonym: MLBr_01226

Alternate gene names: 221230102

Gene position: 1447795-1449378 (Clockwise)

Preceding gene: 221230101

Following gene: 221230103

Centisome position: 44.3

GC content: 64.9

Gene sequence:

>1584_bases
ATGATGGCCGTTCCCGACTGGAAGCACGCGGCTGACGTGGTGGTAATAGGAACCGGCGTGGCGGGCTTGGCTGCCGCGCT
TGCCGCCCACCGCGCCGGTCGCAACGTCGTCGTACTTACCAAAGCCGATCAGCGGCGCGGCGAGACAGCGACGCACTACG
CGCAAGGCGGCATCGCAGTCGTGTTACCGGGTTCCGACGATTCTGTTGACGCCCATGCTTCCGACACCCTGGCGGCAGGG
GCAGGCATGTGCAATCTCGACACCGTGTATTCGATTGTAGCCGAAGGCTACCACGCGGTTACCGAATTAGTCGGTTACGG
AGCGCGTTTCGACGAATCGATTCCCGGGCGATGGGCGGTGACGTGTGAAGGAGGGCACTCGCGACGACGCATCGTGCACG
CCGGTGGCGATGCCACCGGCGCCGAGGTGCAGCGGGCGCTCGACCACGCCGCTGACGTGTTGGACATCCGCACTAGCCAC
CTTGCCCTGCGAGTGTTGCACGATGGCACCGTGGTGAACGGTGTGTCGGTGCTCAACCCAAACGGGTGGGGTATCGTCAG
CGCACCTTCGGTGATCCTGGCCTCCGGTGGGCTCGGGCACCTCTACGGCGCGACCACCAATCCTGAGGGGTCCACTGGCG
ACGGAATCGCGCTGGCGCTGTGGGCTGGCGTGGCGGTTAGCGATCTCGAGTTCATCCAATTCCACCCGACCATGCTTTTC
GCGGCGGGTACTGGCACCGGTGGTCGGCGGCCACTAGTAACCGAAGCCATCCGCGGTGAAGGTGCGATTTTGCTTGATAG
CAAAGGTAATTCGGTAACATCAGGGGTGCATCCACTGGGTGATCTGGCGCCGCGCGATGTTGTCGCTGCTGCTATCGACG
CGCGCCTGAAGGCCACTGGCGATTCGTGCGTCTACCTCGACGCCCGCGGCATCGACGGCTTCGCTTCCCGGTTCCCCACC
GTCACCGCCGCATGCCGGACTGTTGGTATCGACCCGGCCCGTGAACCGATCCCGGTTGTTCCGGGCGCACACTACAGCTG
CGGTGGCATCGTCACCGATGCGTACGGTCAAACCGAGCTGGCTGGGCTGTTCGCCGCTGGAGAGGTGGCCCGCACCGGGA
TGCACGGTGCTAACCGTTTGGCGTCCAACAGCTTGTTGGAAGGCTTGGTGGTCGGTGGCCGCGCCGGAAGGGCTGCGGCC
GCACATGCGGCGGCGGCTGGGCGTGCGTATGTATCCAAGCTCGAGCCTGTCACCCATCACGCGCTGAAGCGCCGTGAACT
CCAACGCGTGATGAGCCGAGATGCCGCGGTGATGCGCAACGCCGCAGGTCTGCAGCGGTTGTCCGACACGCTGGCTGAGG
CGCCGATCCGCCACGTGACCGGCCGCCGCGATTTCGAAGACGTTGCGTTGACGCTGACTGCCCGGGCAGTAGCAGCTGCG
GCCTTGGCCCGCAATGAAAGTCGGGGTTGTCACCACTGCACAGAGTATCCGGACACCGCGCCAGAACACGCCCGCAGCAC
TGTGATTCGACTGGCTGACGACCAAAACTTGGTGCGTGCGGAGGCATTGGCGACGGTGGGTTGA

Upstream 100 bases:

>100_bases
TCGAGGGGACCGACGAGGTTCACGTCGACTCGGAAATCGCGGCGGCGGGCCGGCGCAGTGTGCAGCGTATGATCGAAATC
GGGCTGCCTGGCGGTGGCGA

Downstream 100 bases:

>100_bases
TAATGCTGTCCGACTGCGAATTCGATGCGGCGCGAGACACTATTCGGCGCGCTCTGCATGAGGATCTACGGTACGGGCTC
GACATCACTACGCAGGCCAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MMAVPDWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKADQRRGETATHYAQGGIAVVLPGSDDSVDAHASDTLAAG
AGMCNLDTVYSIVAEGYHAVTELVGYGARFDESIPGRWAVTCEGGHSRRRIVHAGGDATGAEVQRALDHAADVLDIRTSH
LALRVLHDGTVVNGVSVLNPNGWGIVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQFHPTMLF
AAGTGTGGRRPLVTEAIRGEGAILLDSKGNSVTSGVHPLGDLAPRDVVAAAIDARLKATGDSCVYLDARGIDGFASRFPT
VTAACRTVGIDPAREPIPVVPGAHYSCGGIVTDAYGQTELAGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAA
AHAAAAGRAYVSKLEPVTHHALKRRELQRVMSRDAAVMRNAAGLQRLSDTLAEAPIRHVTGRRDFEDVALTLTARAVAAA
ALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEALATVG

Sequences:

>Translated_527_residues
MMAVPDWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKADQRRGETATHYAQGGIAVVLPGSDDSVDAHASDTLAAG
AGMCNLDTVYSIVAEGYHAVTELVGYGARFDESIPGRWAVTCEGGHSRRRIVHAGGDATGAEVQRALDHAADVLDIRTSH
LALRVLHDGTVVNGVSVLNPNGWGIVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQFHPTMLF
AAGTGTGGRRPLVTEAIRGEGAILLDSKGNSVTSGVHPLGDLAPRDVVAAAIDARLKATGDSCVYLDARGIDGFASRFPT
VTAACRTVGIDPAREPIPVVPGAHYSCGGIVTDAYGQTELAGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAA
AHAAAAGRAYVSKLEPVTHHALKRRELQRVMSRDAAVMRNAAGLQRLSDTLAEAPIRHVTGRRDFEDVALTLTARAVAAA
ALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEALATVG
>Mature_527_residues
MMAVPDWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKADQRRGETATHYAQGGIAVVLPGSDDSVDAHASDTLAAG
AGMCNLDTVYSIVAEGYHAVTELVGYGARFDESIPGRWAVTCEGGHSRRRIVHAGGDATGAEVQRALDHAADVLDIRTSH
LALRVLHDGTVVNGVSVLNPNGWGIVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQFHPTMLF
AAGTGTGGRRPLVTEAIRGEGAILLDSKGNSVTSGVHPLGDLAPRDVVAAAIDARLKATGDSCVYLDARGIDGFASRFPT
VTAACRTVGIDPAREPIPVVPGAHYSCGGIVTDAYGQTELAGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAA
AHAAAAGRAYVSKLEPVTHHALKRRELQRVMSRDAAVMRNAAGLQRLSDTLAEAPIRHVTGRRDFEDVALTLTARAVAAA
ALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEALATVG

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=549, Percent_Identity=30.2367941712204, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1788928, Length=540, Percent_Identity=37.5925925925926, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1790597, Length=526, Percent_Identity=32.5095057034221, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1786942, Length=578, Percent_Identity=32.3529411764706, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=533, Percent_Identity=29.8311444652908, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=539, Percent_Identity=28.3858998144712, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71986328, Length=438, Percent_Identity=25.7990867579909, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=577, Percent_Identity=29.9826689774697, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322416, Length=561, Percent_Identity=31.729055258467, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320788, Length=476, Percent_Identity=28.1512605042017, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137288, Length=550, Percent_Identity=31.2727272727273, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24655642, Length=550, Percent_Identity=31.2727272727273, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24655647, Length=550, Percent_Identity=31.2727272727273, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24663005, Length=548, Percent_Identity=29.014598540146, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54314; Mature: 54314

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAVPDWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKADQRRGETATHYAQGGIAV
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCEEE
VLPGSDDSVDAHASDTLAAGAGMCNLDTVYSIVAEGYHAVTELVGYGARFDESIPGRWAV
EECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
TCEGGHSRRRIVHAGGDATGAEVQRALDHAADVLDIRTSHLALRVLHDGTVVNGVSVLNP
EECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEECCEEEECC
NGWGIVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQFHPTMLF
CCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHCCHHCCCCEEEECCEEEE
AAGTGTGGRRPLVTEAIRGEGAILLDSKGNSVTSGVHPLGDLAPRDVVAAAIDARLKATG
EECCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECC
DSCVYLDARGIDGFASRFPTVTAACRTVGIDPAREPIPVVPGAHYSCGGIVTDAYGQTEL
CCEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHH
AGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAAAHAAAAGRAYVSKLEPVTHH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
ALKRRELQRVMSRDAAVMRNAAGLQRLSDTLAEAPIRHVTGRRDFEDVALTLTARAVAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHH
ALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEALATVG
HHHCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MMAVPDWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKADQRRGETATHYAQGGIAV
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCEEE
VLPGSDDSVDAHASDTLAAGAGMCNLDTVYSIVAEGYHAVTELVGYGARFDESIPGRWAV
EECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
TCEGGHSRRRIVHAGGDATGAEVQRALDHAADVLDIRTSHLALRVLHDGTVVNGVSVLNP
EECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEECCEEEECC
NGWGIVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQFHPTMLF
CCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHCCHHCCCCEEEECCEEEE
AAGTGTGGRRPLVTEAIRGEGAILLDSKGNSVTSGVHPLGDLAPRDVVAAAIDARLKATG
EECCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECC
DSCVYLDARGIDGFASRFPTVTAACRTVGIDPAREPIPVVPGAHYSCGGIVTDAYGQTEL
CCEEEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHH
AGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAAAHAAAAGRAYVSKLEPVTHH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
ALKRRELQRVMSRDAAVMRNAAGLQRLSDTLAEAPIRHVTGRRDFEDVALTLTARAVAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHH
ALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEALATVG
HHHCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]