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The map label for this gene is bioD

Identifier: 221230096

GI number: 221230096

Start: 1441095

End: 1441775

Strand: Direct

Name: bioD

Synonym: MLBr_01218

Alternate gene names: 221230096

Gene position: 1441095-1441775 (Clockwise)

Preceding gene: 221230095

Following gene: 221230097

Centisome position: 44.1

GC content: 64.32

Gene sequence:

>681_bases
TTGACTGTCGTGGTCGTTACCGGCACTGACACGGGGGTCGGCAAGACCGTCGCCTGTGCAGCGCTGGCGTGTCACGCGCG
TCAAGCTGGTATCGAAGTGGCAGTGTGCAAACCGGTTCAGACCGGAACTCAGATCGGAGACGATGACCTTGCCGAGGTGG
CCCGACTATCTGGGGTGACCGAACTCACCGGCTTGGTGAGATATCCGCAACCACTGGCGCCGGCCGCCGCCGCCGAACAC
GCCGGGATGGCATTACCCACCCGCGAGCAACTGCTGGAGCTCATCGCCGGACTGGATCGCCCGGGACGCTTGATTTTGGT
CGAAGGCGCTGGTGGTCTACTGGTCGAACTCGCTGATGCTAGTGCCACTTTGCGTGATCTAGCTGTCGAGCTAGGTGCAC
TGGCACTGGTCACGGTCAGTGTGGAATTGGGCACCCTCAACCACACCGCGCTGACTCTGGAGGCGTTGACCACACGAGGG
GTTGCGTGCGCCGGCTTGGTGATCGGCAGCTGGACGGGGCGGCCCGGTGCGGTGCAGATATCCAATCGGTCTGCGCTGGC
CCGGCTGGCTCCAATGCGGGCCACGTTGCCTGCCGGGGCCGGATCGATGGACGCCACGGATTTTGCGGCGATGAGCGCGG
CGGCCTTCGACCGGGACTGGGTAACCACGTTGGTCCATTGA

Upstream 100 bases:

>100_bases
GGCTGCGGTTGACTGCGCACGCGTCTCTCGATAGCGCTAAGTTGGAAGTGGCCCGGCGGGTGTTGACGGACGTTCTTGGT
GGCTGTTGTGTGGCGCGCCG

Downstream 100 bases:

>100_bases
TGGTTCATTCGCTCGAGCTGGTCTTCGACCCCGACACCGAGTCGGCGATCTGTCGCATTTGGGACCAGCTGGCCAGCGCC
GGAATACCCAGCCAAGCGTC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTVVVVTGTDTGVGKTVACAALACHARQAGIEVAVCKPVQTGTQIGDDDLAEVARLSGVTELTGLVRYPQPLAPAAAAEH
AGMALPTREQLLELIAGLDRPGRLILVEGAGGLLVELADASATLRDLAVELGALALVTVSVELGTLNHTALTLEALTTRG
VACAGLVIGSWTGRPGAVQISNRSALARLAPMRATLPAGAGSMDATDFAAMSAAAFDRDWVTTLVH

Sequences:

>Translated_226_residues
MTVVVVTGTDTGVGKTVACAALACHARQAGIEVAVCKPVQTGTQIGDDDLAEVARLSGVTELTGLVRYPQPLAPAAAAEH
AGMALPTREQLLELIAGLDRPGRLILVEGAGGLLVELADASATLRDLAVELGALALVTVSVELGTLNHTALTLEALTTRG
VACAGLVIGSWTGRPGAVQISNRSALARLAPMRATLPAGAGSMDATDFAAMSAAAFDRDWVTTLVH
>Mature_225_residues
TVVVVTGTDTGVGKTVACAALACHARQAGIEVAVCKPVQTGTQIGDDDLAEVARLSGVTELTGLVRYPQPLAPAAAAEHA
GMALPTREQLLELIAGLDRPGRLILVEGAGGLLVELADASATLRDLAVELGALALVTVSVELGTLNHTALTLEALTTRGV
ACAGLVIGSWTGRPGAVQISNRSALARLAPMRATLPAGAGSMDATDFAAMSAAAFDRDWVTTLVH

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=199, Percent_Identity=31.1557788944724, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_MYCLB (B8ZR85)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503512.1
- ProteinModelPortal:   B8ZR85
- SMR:   B8ZR85
- EnsemblBacteria:   EBMYCT00000085642
- GeneID:   7327264
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017652
- HOGENOM:   HBG650065
- OMA:   VVIGSWP
- ProtClustDB:   PRK00090
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 22924; Mature: 22792

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVVVTGTDTGVGKTVACAALACHARQAGIEVAVCKPVQTGTQIGDDDLAEVARLSGVT
CEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHH
ELTGLVRYPQPLAPAAAAEHAGMALPTREQLLELIAGLDRPGRLILVEGAGGLLVELADA
HHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCC
SATLRDLAVELGALALVTVSVELGTLNHTALTLEALTTRGVACAGLVIGSWTGRPGAVQI
HHHHHHHHHHHCCEEEEEEEEEECCCCCCEEEEEHHHHCCCEEEEEEEECCCCCCCEEEE
SNRSALARLAPMRATLPAGAGSMDATDFAAMSAAAFDRDWVTTLVH
CCCHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TVVVVTGTDTGVGKTVACAALACHARQAGIEVAVCKPVQTGTQIGDDDLAEVARLSGVT
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHH
ELTGLVRYPQPLAPAAAAEHAGMALPTREQLLELIAGLDRPGRLILVEGAGGLLVELADA
HHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCC
SATLRDLAVELGALALVTVSVELGTLNHTALTLEALTTRGVACAGLVIGSWTGRPGAVQI
HHHHHHHHHHHCCEEEEEEEEEECCCCCCEEEEEHHHHCCCEEEEEEEECCCCCCCEEEE
SNRSALARLAPMRATLPAGAGSMDATDFAAMSAAAFDRDWVTTLVH
CCCHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA