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The map label for this gene is bioB

Identifier: 221230097

GI number: 221230097

Start: 1442529

End: 1443566

Strand: Direct

Name: bioB

Synonym: MLBr_01220

Alternate gene names: 221230097

Gene position: 1442529-1443566 (Clockwise)

Preceding gene: 221230096

Following gene: 221230098

Centisome position: 44.14

GC content: 59.54

Gene sequence:

>1038_bases
GTGACTCAGGCGGGGACTCGGTCAACTAATGGTGATACCGAAAATACTGACATCTTGGCTCAGGCTCGCCAGCAGGTGCT
AGAGCGCGGTGAGGGCTTGAGCCGGGACCAGGTGCTGCAGATGCTGTGGCTACCCGACGACCGACTCGAGGAATTGCTGG
CGTTAGCCCACGACGTGCGGATGCGCTGGTGCGGTCCAGAGGTCGAGATCGAGGGCATCATCAGTCTGAAAACCGGTGGC
TGCCCAGAGGATTGCCATTTCTGCTCACAGTCTGGGTTGTTCATGTCGCCGGTGCGCAGTGCCTGGCTGGATATCCGCAG
CCTGGTAGAGGCAGCCAAGCAGACTGCCAAGTCAGGTGCCACTGAATTCTGCATCGTGGCAGCGGTACGAGGGCCCGATG
AGCGGTTGATGTCCCAGGTAGCGGCCGGCATTGAGGCGATTCGGAACGAAGTCGAGATTAATATTGCTTGCTCGCTGGGG
ATGCTGACCGTCGAGCAGGTGGAGCAGCTCTCGGGCATTGGCGTGCACCGCTATAACCACAACCTCGAAACAGCCCGCTC
GTTTTTCACCAACGTTGTCACCACGCACACCTGGGAAGAGCGCTGGCAAACGTTATCGATGGTTCGGGACGCCGGCATGG
AGGTATGCTGTGGCGGCATTCTCGGCATGGGGGAGACAGTCGAGCAGCGCGCAGAATTTGCTCTTGAACTCGCCGAACTC
GGCCCTGATGAGGTTCCTTTGAATTTTCTCAACCCGAGGCCCGGTACTCCCTTTGGTGCCCTTGAGGTGATGCCGCCCAG
CGAAGCATTAAAATCGGTGGCTGCATTCCGATTGGCGTTGCCGCGCACGATCCTGCGCTTCGCCGGCGGCCGCGAGATCA
CCCTGGGTGACCTGGGCGCCAAGCAGGGCATGCTGGGCGGCATCAACGCAGTGATCGTGGGGAACTACTTGACCACGTTG
GGTCGGCCCGCCGAAGCCGATTTGAGATTGCTTGACGATCTACAAATGCCTATCAAGGCACTCAATGCCAGCCTATAG

Upstream 100 bases:

>100_bases
GACACCGTTCATGTCAGGCCGCGAAGAGCCTGGTGAACCGCTTCGGTATCCGCAAGCTCGGACCCTAGTTTTAAGACTTG
AACGTGAAAAGGAGTACTGC

Downstream 100 bases:

>100_bases
AAAATGCCTATAGAAAATAAGGACTGTGGTTCAGGATCTTCCTACTCCGATCGGTGCGGGCATCTACAACATCTATACCG
GTGTCAAACACCGGGATGAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTQAGTRSTNGDTENTDILAQARQQVLERGEGLSRDQVLQMLWLPDDRLEELLALAHDVRMRWCGPEVEIEGIISLKTGG
CPEDCHFCSQSGLFMSPVRSAWLDIRSLVEAAKQTAKSGATEFCIVAAVRGPDERLMSQVAAGIEAIRNEVEINIACSLG
MLTVEQVEQLSGIGVHRYNHNLETARSFFTNVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETVEQRAEFALELAEL
GPDEVPLNFLNPRPGTPFGALEVMPPSEALKSVAAFRLALPRTILRFAGGREITLGDLGAKQGMLGGINAVIVGNYLTTL
GRPAEADLRLLDDLQMPIKALNASL

Sequences:

>Translated_345_residues
MTQAGTRSTNGDTENTDILAQARQQVLERGEGLSRDQVLQMLWLPDDRLEELLALAHDVRMRWCGPEVEIEGIISLKTGG
CPEDCHFCSQSGLFMSPVRSAWLDIRSLVEAAKQTAKSGATEFCIVAAVRGPDERLMSQVAAGIEAIRNEVEINIACSLG
MLTVEQVEQLSGIGVHRYNHNLETARSFFTNVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETVEQRAEFALELAEL
GPDEVPLNFLNPRPGTPFGALEVMPPSEALKSVAAFRLALPRTILRFAGGREITLGDLGAKQGMLGGINAVIVGNYLTTL
GRPAEADLRLLDDLQMPIKALNASL
>Mature_344_residues
TQAGTRSTNGDTENTDILAQARQQVLERGEGLSRDQVLQMLWLPDDRLEELLALAHDVRMRWCGPEVEIEGIISLKTGGC
PEDCHFCSQSGLFMSPVRSAWLDIRSLVEAAKQTAKSGATEFCIVAAVRGPDERLMSQVAAGIEAIRNEVEINIACSLGM
LTVEQVEQLSGIGVHRYNHNLETARSFFTNVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETVEQRAEFALELAELG
PDEVPLNFLNPRPGTPFGALEVMPPSEALKSVAAFRLALPRTILRFAGGREITLGDLGAKQGMLGGINAVIVGNYLTTLG
RPAEADLRLLDDLQMPIKALNASL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=35.5048859934853, Blast_Score=185, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321725, Length=334, Percent_Identity=28.1437125748503, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_MYCLB (B8ZR86)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503513.1
- ProteinModelPortal:   B8ZR86
- SMR:   B8ZR86
- EnsemblBacteria:   EBMYCT00000086505
- GeneID:   7327079
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017018
- HOGENOM:   HBG405569
- OMA:   ATGATEF
- ProtClustDB:   PRK06256
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37559; Mature: 37427

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAGTRSTNGDTENTDILAQARQQVLERGEGLSRDQVLQMLWLPDDRLEELLALAHDVR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MRWCGPEVEIEGIISLKTGGCPEDCHFCSQSGLFMSPVRSAWLDIRSLVEAAKQTAKSGA
HHHCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TEFCIVAAVRGPDERLMSQVAAGIEAIRNEVEINIACSLGMLTVEQVEQLSGIGVHRYNH
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHCCCEEEECC
NLETARSFFTNVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETVEQRAEFALELAEL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GPDEVPLNFLNPRPGTPFGALEVMPPSEALKSVAAFRLALPRTILRFAGGREITLGDLGA
CCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KQGMLGGINAVIVGNYLTTLGRPAEADLRLLDDLQMPIKALNASL
CCCCCCCCHHEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQAGTRSTNGDTENTDILAQARQQVLERGEGLSRDQVLQMLWLPDDRLEELLALAHDVR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MRWCGPEVEIEGIISLKTGGCPEDCHFCSQSGLFMSPVRSAWLDIRSLVEAAKQTAKSGA
HHHCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TEFCIVAAVRGPDERLMSQVAAGIEAIRNEVEINIACSLGMLTVEQVEQLSGIGVHRYNH
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHCCCEEEECC
NLETARSFFTNVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETVEQRAEFALELAEL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GPDEVPLNFLNPRPGTPFGALEVMPPSEALKSVAAFRLALPRTILRFAGGREITLGDLGA
CCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KQGMLGGINAVIVGNYLTTLGRPAEADLRLLDDLQMPIKALNASL
CCCCCCCCHHEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA