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The map label for this gene is asnB [H]

Identifier: 220933073

GI number: 220933073

Start: 2441788

End: 2443242

Strand: Reverse

Name: asnB [H]

Synonym: Hore_22410

Alternate gene names: 220933073

Gene position: 2443242-2441788 (Counterclockwise)

Preceding gene: 220933075

Following gene: 220933072

Centisome position: 94.77

GC content: 41.37

Gene sequence:

>1455_bases
ATGAGTGGCATTGCAGGAGTTTACGAGGATGATGACCCCGGACTGGTGAGAGATGTCCTAAAAAAGATGGAACACCGGGG
TCCAGAAAGTACCAGAATTTATAAGGCCCCGAAGGGGGTTCTGGGATGTACCCGGATTAAGGGTAAGGGGGATTATTCCC
CGAAAGAGGTTATAGCTATTTCCGATGGCAGGGTGGTAGTTGAAGGAAAAAAAGACAGTTCAGGGTATGGACAATGGGTT
AAAGGCAATAGTGAAATAATCGAACTATATAATAAATTTGGGAAGGAATGTGTTCACTACTTTAATGGCCCCTTTGCTAT
TGCTATTTTTGACGGGGAAAATATTTTTGTGGCCCGGGATCCTTTAGGATTGAAACCCCTGTACTATAGTAATATTGGTG
AAAAATTTGTATTTGCCTCTGAAATTAAGGGTCTGGCCCAGTTTGATTCAGAGATTAAAACCTTCCCTCCCGGACACTAT
TATCATTCTGAAGTAGGGTTTCAGGAATATTTTAAAGGTGTATCCCATAAACAGAGGATAACTTCAGTCGATAAAGGGGT
AAAAAGGCTGCGTAATTTACTTATTAAAACGGTAAAGAGGAGAATTGGTGGAATTGATAACCCTGGCATATATTTAAGTG
GAGGGATTGATAGCAGTACTATTGTGGCTGCAGCTTCGAAAGTTAAGGAAGTTATTAAAACCTTTGCAGTCGGGGCTAAA
GGGAGTAATGATCTGGAAAAAGCCAGGCTGGTGGCAGACCATCTGGGAACCGACCACTATGAATACCGGTATACTGTAGA
AGAGGCTCTGGAAATAGTCCCTGAGGTTATTTATCAGCTGGAATCATTTGACCAGTATCTGGTCCGCAGTTCAATTGCCA
ACTATTTTGTTGGCAGGCTGGCCAAAGAGACGGGGGTTGAACTGGTACTATGTGGTGAAGGTGGCGATGAGTTATTTGGT
GGTTATCACTATTTAAAGGACATGCAAAGCCTTGCTGATATCAGGGAAGAGCTTGAAGTTCTAACAATGTCGGGCCATTC
CAATGGTTTTCAGAGGGTAGATAGAATGAATATGGCCCATTCCCTGTTATATGATATGCCTTTTATGGACAAAGAGATTG
TAAAATTTGCCTTCAGTATTCCGGTAAAGTGGAAGCTTTATGGTAATAAACCGGTGGAGAAATGGATTTTAAGGAAGGCC
TTTGAGGATGACCTTCCTGATGAGATTATCTGGCGAAAGAAGGCTAAATTTTTTGAGGGGTCAAATACCACTGATACTAT
AAAGAGCAAAATAGAGTCATTTATAAGTGATAAAGAGTTTAAAAAGGAAAAAAAGATTTCTGATGATGTTACCCTCAGGA
GTAAAGAAGAGCTTTATTACTACCGAATCTTCAGGGAGTATTATCCCCACCGGTCAATCCTGGATACCATCGGAAGGACC
CGGACTGTAAATTAA

Upstream 100 bases:

>100_bases
TATCTTAAAATTTTTGAGAAAAAAAGGAAGGAATTTTTATTATTATCTTTAAATGATAATAAAAAGGTAATAAAAACCAG
GGAGGTATTACACTAACACA

Downstream 100 bases:

>100_bases
GATAAGGAAGGTCCTACAGGCCCCTGTTACAGGGGCCTTTGTTTAATTTATACTTGTTTAATGTGGCGAATGGCTTTTAC
AGGAACGGAGGAATGGTCAG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSGIAGVYEDDDPGLVRDVLKKMEHRGPESTRIYKAPKGVLGCTRIKGKGDYSPKEVIAISDGRVVVEGKKDSSGYGQWV
KGNSEIIELYNKFGKECVHYFNGPFAIAIFDGENIFVARDPLGLKPLYYSNIGEKFVFASEIKGLAQFDSEIKTFPPGHY
YHSEVGFQEYFKGVSHKQRITSVDKGVKRLRNLLIKTVKRRIGGIDNPGIYLSGGIDSSTIVAAASKVKEVIKTFAVGAK
GSNDLEKARLVADHLGTDHYEYRYTVEEALEIVPEVIYQLESFDQYLVRSSIANYFVGRLAKETGVELVLCGEGGDELFG
GYHYLKDMQSLADIREELEVLTMSGHSNGFQRVDRMNMAHSLLYDMPFMDKEIVKFAFSIPVKWKLYGNKPVEKWILRKA
FEDDLPDEIIWRKKAKFFEGSNTTDTIKSKIESFISDKEFKKEKKISDDVTLRSKEELYYYRIFREYYPHRSILDTIGRT
RTVN

Sequences:

>Translated_484_residues
MSGIAGVYEDDDPGLVRDVLKKMEHRGPESTRIYKAPKGVLGCTRIKGKGDYSPKEVIAISDGRVVVEGKKDSSGYGQWV
KGNSEIIELYNKFGKECVHYFNGPFAIAIFDGENIFVARDPLGLKPLYYSNIGEKFVFASEIKGLAQFDSEIKTFPPGHY
YHSEVGFQEYFKGVSHKQRITSVDKGVKRLRNLLIKTVKRRIGGIDNPGIYLSGGIDSSTIVAAASKVKEVIKTFAVGAK
GSNDLEKARLVADHLGTDHYEYRYTVEEALEIVPEVIYQLESFDQYLVRSSIANYFVGRLAKETGVELVLCGEGGDELFG
GYHYLKDMQSLADIREELEVLTMSGHSNGFQRVDRMNMAHSLLYDMPFMDKEIVKFAFSIPVKWKLYGNKPVEKWILRKA
FEDDLPDEIIWRKKAKFFEGSNTTDTIKSKIESFISDKEFKKEKKISDDVTLRSKEELYYYRIFREYYPHRSILDTIGRT
RTVN
>Mature_483_residues
SGIAGVYEDDDPGLVRDVLKKMEHRGPESTRIYKAPKGVLGCTRIKGKGDYSPKEVIAISDGRVVVEGKKDSSGYGQWVK
GNSEIIELYNKFGKECVHYFNGPFAIAIFDGENIFVARDPLGLKPLYYSNIGEKFVFASEIKGLAQFDSEIKTFPPGHYY
HSEVGFQEYFKGVSHKQRITSVDKGVKRLRNLLIKTVKRRIGGIDNPGIYLSGGIDSSTIVAAASKVKEVIKTFAVGAKG
SNDLEKARLVADHLGTDHYEYRYTVEEALEIVPEVIYQLESFDQYLVRSSIANYFVGRLAKETGVELVLCGEGGDELFGG
YHYLKDMQSLADIREELEVLTMSGHSNGFQRVDRMNMAHSLLYDMPFMDKEIVKFAFSIPVKWKLYGNKPVEKWILRKAF
EDDLPDEIIWRKKAKFFEGSNTTDTIKSKIESFISDKEFKKEKKISDDVTLRSKEELYYYRIFREYYPHRSILDTIGRTR
TVN

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010852, Length=431, Percent_Identity=30.1624129930394, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI296010850, Length=431, Percent_Identity=30.1624129930394, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI168229248, Length=532, Percent_Identity=28.3834586466165, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI168229252, Length=532, Percent_Identity=28.3834586466165, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI168229250, Length=532, Percent_Identity=28.3834586466165, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI296010848, Length=431, Percent_Identity=30.1624129930394, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786889, Length=493, Percent_Identity=32.0486815415822, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25147557, Length=499, Percent_Identity=32.064128256513, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71993933, Length=507, Percent_Identity=31.7554240631164, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17560178, Length=392, Percent_Identity=32.9081632653061, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25147560, Length=293, Percent_Identity=29.3515358361775, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321563, Length=548, Percent_Identity=30.1094890510949, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6325403, Length=547, Percent_Identity=29.6160877513711, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45553209, Length=499, Percent_Identity=31.2625250501002, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 55095; Mature: 54963

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIAGVYEDDDPGLVRDVLKKMEHRGPESTRIYKAPKGVLGCTRIKGKGDYSPKEVIAI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCEEEEE
SDGRVVVEGKKDSSGYGQWVKGNSEIIELYNKFGKECVHYFNGPFAIAIFDGENIFVARD
ECCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECC
PLGLKPLYYSNIGEKFVFASEIKGLAQFDSEIKTFPPGHYYHSEVGFQEYFKGVSHKQRI
CCCCCCHHHHCCCCHHEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHH
TSVDKGVKRLRNLLIKTVKRRIGGIDNPGIYLSGGIDSSTIVAAASKVKEVIKTFAVGAK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
GSNDLEKARLVADHLGTDHYEYRYTVEEALEIVPEVIYQLESFDQYLVRSSIANYFVGRL
CCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKETGVELVLCGEGGDELFGGYHYLKDMQSLADIREELEVLTMSGHSNGFQRVDRMNMAH
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHH
SLLYDMPFMDKEIVKFAFSIPVKWKLYGNKPVEKWILRKAFEDDLPDEIIWRKKAKFFEG
HHHHCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
SNTTDTIKSKIESFISDKEFKKEKKISDDVTLRSKEELYYYRIFREYYPHRSILDTIGRT
CCCHHHHHHHHHHHHCHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHCCCHHHHHHCCCC
RTVN
CCCC
>Mature Secondary Structure 
SGIAGVYEDDDPGLVRDVLKKMEHRGPESTRIYKAPKGVLGCTRIKGKGDYSPKEVIAI
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCEEEEE
SDGRVVVEGKKDSSGYGQWVKGNSEIIELYNKFGKECVHYFNGPFAIAIFDGENIFVARD
ECCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECC
PLGLKPLYYSNIGEKFVFASEIKGLAQFDSEIKTFPPGHYYHSEVGFQEYFKGVSHKQRI
CCCCCCHHHHCCCCHHEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHH
TSVDKGVKRLRNLLIKTVKRRIGGIDNPGIYLSGGIDSSTIVAAASKVKEVIKTFAVGAK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
GSNDLEKARLVADHLGTDHYEYRYTVEEALEIVPEVIYQLESFDQYLVRSSIANYFVGRL
CCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKETGVELVLCGEGGDELFGGYHYLKDMQSLADIREELEVLTMSGHSNGFQRVDRMNMAH
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHH
SLLYDMPFMDKEIVKFAFSIPVKWKLYGNKPVEKWILRKAFEDDLPDEIIWRKKAKFFEG
HHHHCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
SNTTDTIKSKIESFISDKEFKKEKKISDDVTLRSKEELYYYRIFREYYPHRSILDTIGRT
CCCHHHHHHHHHHHHCHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHCCCHHHHHHCCCC
RTVN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]