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The map label for this gene is leuD

Identifier: 220931929

GI number: 220931929

Start: 1182581

End: 1183078

Strand: Direct

Name: leuD

Synonym: Hore_10880

Alternate gene names: 220931929

Gene position: 1182581-1183078 (Clockwise)

Preceding gene: 220931928

Following gene: 220931930

Centisome position: 45.87

GC content: 37.15

Gene sequence:

>498_bases
ATGAAACTTAAGGGAAGAGTACTTAAATATGGTGATAATGTTGATACTGATGTAATTATTCCGGCACGTTACCTGAATAC
ATCAAACCCGGATGAGCTTGCCCGTCATTGCATGGAGGATATAGACCCTGATTTTGCCAGTAAAGTAAAGGATGGAGATA
TTATAGTTGCTGGAAAAAATTTCGGTTCAGGTAGTTCCAGGGAACATGCCCCCCTCGCTATAAAATCGGCTGGGGTCTCC
TGTGTTATTGCGGAGAGTTTTGCCAGGATTTTTTATCGAAATGCAATCAACATTGGTTTACCTATTTTAGAATGCAAGGA
TGTTACTGACAGGATAGAAGAGGGGGACTTAGTAGAAGTTGATTTAAATGAAGGTAAAATTAAGTTACCAGAAAAAAATA
TAAATATTGATTCAACACCCTTTCCCTCTTTTATGCAGGAAATTATTAAAGCAGGTGGGTTAATTGAAAAAATTAAAGGG
GGAATAAAACATGCTTAA

Upstream 100 bases:

>100_bases
GAATGGGTCATCCTGAAAGTGAAGTTTATCTAGCTAATCCTGCAGTAGCCGCTGCTTCTGCTATAAAAGGGTATATAGCC
CACCCGGAGGAGGTTTTATA

Downstream 100 bases:

>100_bases
AATTGCTGTAATACCCGGGGATGGAATTGGAAAAGAGGTTACCGATCAGGGTCTTAAGGTTATGGAATATTTAAATGGTA
AAAATGGATTACAGTTAACC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKLKGRVLKYGDNVDTDVIIPARYLNTSNPDELARHCMEDIDPDFASKVKDGDIIVAGKNFGSGSSREHAPLAIKSAGVS
CVIAESFARIFYRNAINIGLPILECKDVTDRIEEGDLVEVDLNEGKIKLPEKNINIDSTPFPSFMQEIIKAGGLIEKIKG
GIKHA

Sequences:

>Translated_165_residues
MKLKGRVLKYGDNVDTDVIIPARYLNTSNPDELARHCMEDIDPDFASKVKDGDIIVAGKNFGSGSSREHAPLAIKSAGVS
CVIAESFARIFYRNAINIGLPILECKDVTDRIEEGDLVEVDLNEGKIKLPEKNINIDSTPFPSFMQEIIKAGGLIEKIKG
GIKHA
>Mature_165_residues
MKLKGRVLKYGDNVDTDVIIPARYLNTSNPDELARHCMEDIDPDFASKVKDGDIIVAGKNFGSGSSREHAPLAIKSAGVS
CVIAESFARIFYRNAINIGLPILECKDVTDRIEEGDLVEVDLNEGKIKLPEKNINIDSTPFPSFMQEIIKAGGLIEKIKG
GIKHA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=141, Percent_Identity=35.4609929078014, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=174, Percent_Identity=34.4827586206897, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=161, Percent_Identity=33.5403726708075, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_HALOH (B8CX23)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508837.1
- ProteinModelPortal:   B8CX23
- SMR:   B8CX23
- GeneID:   7312830
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_10880
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02084
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18040; Mature: 18040

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKGRVLKYGDNVDTDVIIPARYLNTSNPDELARHCMEDIDPDFASKVKDGDIIVAGKN
CCCCCCEEEECCCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEECCC
FGSGSSREHAPLAIKSAGVSCVIAESFARIFYRNAINIGLPILECKDVTDRIEEGDLVEV
CCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCCCCEEEE
DLNEGKIKLPEKNINIDSTPFPSFMQEIIKAGGLIEKIKGGIKHA
ECCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure
MKLKGRVLKYGDNVDTDVIIPARYLNTSNPDELARHCMEDIDPDFASKVKDGDIIVAGKN
CCCCCCEEEECCCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEECCC
FGSGSSREHAPLAIKSAGVSCVIAESFARIFYRNAINIGLPILECKDVTDRIEEGDLVEV
CCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCEEECCCHHHHCCCCCEEEE
DLNEGKIKLPEKNINIDSTPFPSFMQEIIKAGGLIEKIKGGIKHA
ECCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA