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The map label for this gene is leuC

Identifier: 220931928

GI number: 220931928

Start: 1181319

End: 1182581

Strand: Direct

Name: leuC

Synonym: Hore_10870

Alternate gene names: 220931928

Gene position: 1181319-1182581 (Clockwise)

Preceding gene: 220931927

Following gene: 220931929

Centisome position: 45.82

GC content: 42.04

Gene sequence:

>1263_bases
ATGGGTATGACTATGATTGAAAAAATAATTGCGGCCCATACCAATAGAGATAAGGTAAAACCAGGTGATATAGTGAATGC
CAGGATTGACTTTGTCCTGGGAAATGATGTCACCACTCCTGTTGCCGTAAAAGAATTCAATAAGATCGGGATTGATGAGG
TCTTTGATCAGGATAGAATAGCTATTGTACCAGACCATTTTACCCCCAATAAAGATATTAAATCTGCTGAACAGTGTAAA
TTTATTAGGGAATTTGCCCGGGATAAAGGGATTCATAATTACTTTGAAATAGGAGAAATGGGGATTGAACACTGTCTTTT
GCCCGAAAAGGGGCTTGCTTTACCAGGTGAAATAATAATTGGAGCTGATTCTCATACCTGTACTTATGGGGCCCTGGGGG
CTTTTGCTACCGGAGTTGGGAGTACCGATATGGCAGCAGCTATGGCAACAGGTTATACCTGGTTTAAAGTTCCATCGACT
ATCCAATTTGTTTATAATGGGGAACTTAAACCCTGGGTCAGTGGTAAGGATTTAATCCTGTATACTATTGGTGATATTGG
TGTTGACGGGGCCCTTTACAGGGCCATGGAGTTTACAGGGGAAACCATTGAAAATCTTTCCATGGATAATAGAATGACCA
TATCCAACATGGCCATTGAGGCCGGTGGGAAATGTGGACTTATCGCTCCTGACGAAAAAACCTTTAAATATCTAGAAAAT
AGGGCTCAAAGGGATTATACACCATGTTACAGCGATGATGATGCTGAATATGAAAAGGTTATAGAATATGATGTCAGTGA
TATTGAGCCCCAGGTTGCATTTCCCCATTTACCTGAAAACACCAGGCCTATTAGTGAGGAAGGTGATATAAAAATTGATC
AGGCAGTAATCGGTTCCTGTACAAATGGTAGAATGGAGGATTTAAGGATAGCAGCCGGTATTTTAAAGGAGAAACAGGTC
CATCATGATGTCAGGTGTATTGTAATACCGGGTACTCAGGATATTTATAAGCAGGCTCTCAAGGAAGGACTTATTGAAAT
CTTTATTGAGGCCGGTGCAGCTGTCAGTACACCTACCTGTGGTCCCTGTCTGGGAGGTCACATGGGTATACTGGCCCGTG
GAGAAAAGGCCATTGCTACAACAAATCGTAATTTTGTGGGGAGAATGGGTCATCCTGAAAGTGAAGTTTATCTAGCTAAT
CCTGCAGTAGCCGCTGCTTCTGCTATAAAAGGGTATATAGCCCACCCGGAGGAGGTTTTATAA

Upstream 100 bases:

>100_bases
TGCCCTTGCTTATCTCGAAACCATTAATAAATTTTTAACATCAGAACAAACAAGGCAGACAACTTCTGCACAAGAAGGCA
TTTAAAAGGAGGTTAAAATA

Downstream 100 bases:

>100_bases
TGAAACTTAAGGGAAGAGTACTTAAATATGGTGATAATGTTGATACTGATGTAATTATTCCGGCACGTTACCTGAATACA
TCAAACCCGGATGAGCTTGC

Product: 3-isopropylmalate dehydratase, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MGMTMIEKIIAAHTNRDKVKPGDIVNARIDFVLGNDVTTPVAVKEFNKIGIDEVFDQDRIAIVPDHFTPNKDIKSAEQCK
FIREFARDKGIHNYFEIGEMGIEHCLLPEKGLALPGEIIIGADSHTCTYGALGAFATGVGSTDMAAAMATGYTWFKVPST
IQFVYNGELKPWVSGKDLILYTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYLEN
RAQRDYTPCYSDDDAEYEKVIEYDVSDIEPQVAFPHLPENTRPISEEGDIKIDQAVIGSCTNGRMEDLRIAAGILKEKQV
HHDVRCIVIPGTQDIYKQALKEGLIEIFIEAGAAVSTPTCGPCLGGHMGILARGEKAIATTNRNFVGRMGHPESEVYLAN
PAVAAASAIKGYIAHPEEVL

Sequences:

>Translated_420_residues
MGMTMIEKIIAAHTNRDKVKPGDIVNARIDFVLGNDVTTPVAVKEFNKIGIDEVFDQDRIAIVPDHFTPNKDIKSAEQCK
FIREFARDKGIHNYFEIGEMGIEHCLLPEKGLALPGEIIIGADSHTCTYGALGAFATGVGSTDMAAAMATGYTWFKVPST
IQFVYNGELKPWVSGKDLILYTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYLEN
RAQRDYTPCYSDDDAEYEKVIEYDVSDIEPQVAFPHLPENTRPISEEGDIKIDQAVIGSCTNGRMEDLRIAAGILKEKQV
HHDVRCIVIPGTQDIYKQALKEGLIEIFIEAGAAVSTPTCGPCLGGHMGILARGEKAIATTNRNFVGRMGHPESEVYLAN
PAVAAASAIKGYIAHPEEVL
>Mature_419_residues
GMTMIEKIIAAHTNRDKVKPGDIVNARIDFVLGNDVTTPVAVKEFNKIGIDEVFDQDRIAIVPDHFTPNKDIKSAEQCKF
IREFARDKGIHNYFEIGEMGIEHCLLPEKGLALPGEIIIGADSHTCTYGALGAFATGVGSTDMAAAMATGYTWFKVPSTI
QFVYNGELKPWVSGKDLILYTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYLENR
AQRDYTPCYSDDDAEYEKVIEYDVSDIEPQVAFPHLPENTRPISEEGDIKIDQAVIGSCTNGRMEDLRIAAGILKEKQVH
HDVRCIVIPGTQDIYKQALKEGLIEIFIEAGAAVSTPTCGPCLGGHMGILARGEKAIATTNRNFVGRMGHPESEVYLANP
AVAAASAIKGYIAHPEEVL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=450, Percent_Identity=28.2222222222222, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=22.7154046997389, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI8659555, Length=371, Percent_Identity=23.4501347708895, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=33.4047109207709, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI87081781, Length=347, Percent_Identity=27.3775216138329, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1787531, Length=358, Percent_Identity=24.3016759776536, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI2367097, Length=461, Percent_Identity=24.9457700650759, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=450, Percent_Identity=28.6666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=336, Percent_Identity=30.952380952381, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=29.0780141843972, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=423, Percent_Identity=22.6950354609929, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=34.589800443459, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321429, Length=431, Percent_Identity=33.8747099767981, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6323335, Length=447, Percent_Identity=29.9776286353468, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=458, Percent_Identity=29.0393013100437, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI28571643, Length=445, Percent_Identity=28.0898876404494, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=27.9642058165548, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=27.9642058165548, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161076999, Length=337, Percent_Identity=30.5637982195846, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=24.5945945945946, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24645686, Length=368, Percent_Identity=25.2717391304348, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_HALOH (B8CX22)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508836.1
- ProteinModelPortal:   B8CX22
- SMR:   B8CX22
- GeneID:   7312829
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_10870
- HOGENOM:   HBG330745
- OMA:   PENTKTI
- ProtClustDB:   CLSK2476423
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45854; Mature: 45723

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTMIEKIIAAHTNRDKVKPGDIVNARIDFVLGNDVTTPVAVKEFNKIGIDEVFDQDRI
CCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCHHHHCCCCCE
AIVPDHFTPNKDIKSAEQCKFIREFARDKGIHNYFEIGEMGIEHCLLPEKGLALPGEIII
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
GADSHTCTYGALGAFATGVGSTDMAAAMATGYTWFKVPSTIQFVYNGELKPWVSGKDLIL
ECCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCCCEEE
YTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYLEN
EEECCCCCCHHHHHHHHHCCHHHHHCCCCCCEEEEHEEEECCCCEEEECCCHHHHHHHHH
RAQRDYTPCYSDDDAEYEKVIEYDVSDIEPQVAFPHLPENTRPISEEGDIKIDQAVIGSC
HHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCC
TNGRMEDLRIAAGILKEKQVHHDVRCIVIPGTQDIYKQALKEGLIEIFIEAGAAVSTPTC
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPCLGGHMGILARGEKAIATTNRNFVGRMGHPESEVYLANPAVAAASAIKGYIAHPEEVL
CCCCCCCCCEEECCCEEEEECCCCEEECCCCCCCCEEEECCHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
GMTMIEKIIAAHTNRDKVKPGDIVNARIDFVLGNDVTTPVAVKEFNKIGIDEVFDQDRI
CHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCHHHHCCCCCE
AIVPDHFTPNKDIKSAEQCKFIREFARDKGIHNYFEIGEMGIEHCLLPEKGLALPGEIII
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
GADSHTCTYGALGAFATGVGSTDMAAAMATGYTWFKVPSTIQFVYNGELKPWVSGKDLIL
ECCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCCCEEE
YTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYLEN
EEECCCCCCHHHHHHHHHCCHHHHHCCCCCCEEEEHEEEECCCCEEEECCCHHHHHHHHH
RAQRDYTPCYSDDDAEYEKVIEYDVSDIEPQVAFPHLPENTRPISEEGDIKIDQAVIGSC
HHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCC
TNGRMEDLRIAAGILKEKQVHHDVRCIVIPGTQDIYKQALKEGLIEIFIEAGAAVSTPTC
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPCLGGHMGILARGEKAIATTNRNFVGRMGHPESEVYLANPAVAAASAIKGYIAHPEEVL
CCCCCCCCCEEECCCEEEEECCCCEEECCCCCCCCEEEECCHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA