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The map label for this gene is leuB [H]

Identifier: 220931930

GI number: 220931930

Start: 1183071

End: 1184147

Strand: Direct

Name: leuB [H]

Synonym: Hore_10890

Alternate gene names: 220931930

Gene position: 1183071-1184147 (Clockwise)

Preceding gene: 220931929

Following gene: 220931931

Centisome position: 45.89

GC content: 40.67

Gene sequence:

>1077_bases
ATGCTTAAAATTGCTGTAATACCCGGGGATGGAATTGGAAAAGAGGTTACCGATCAGGGTCTTAAGGTTATGGAATATTT
AAATGGTAAAAATGGATTACAGTTAACCCTTGATAAATTCGAACTGGGTGCTGAAAGGTATCTAAAAAATGGAGAAGTTT
TGCCTGAAAAAGTTTTGAAAAAACTTGAAACATATAATTCTATATATCTGGGGGCAGTGGGGGATCCACGGGTTCCAGCC
GGTGTCCTGGAACAGGGTATATTGTTGAAACTTAGATTTTATTTTGACCAGTATGTTAATCTAAGACCGGTTAAACTTTT
TAAAGATGACTTTTCACCGTTAAAAAACAAAGGGGTTAATGACATTGATTTTACAGTGATCAGGGAAAACACTGAAGGGT
TATATGCCGGGGTAGGTGGATTTTTAAAGAAGAATACCCCTGATGAAGTAGCCATCCAGGAGATGGTTAATACCCGGAAG
GGTGTTGACAGGGTTATAAAATATGCTTTTGAATATGCCCTCAAATATGGAAAAGAACCCAAGGTTACCCTGTGTGATAA
GAGCAATGTATTAACCTATGCCCATAATCTCTGGCAGAGGGCCTTTAAGGATATTGCTGTAACTTATCCCCGGGTTGAAA
CTGACCATTTTCTGGTTGATGCCATTACCATGAAGATGGTAAGAAATCCTGAAATATTTGATGTTATTGTTACCTGTAAT
ATGTTCGGTGATATTATTACAGACCTGGGGGCTGAACTTCAGGGTGGTATGGGCCTGGCCGTTTCCGGAAACATTAACCC
AGACAGTGTTTCCATGTTTGAACCCGTTCATGGTTCTGCTCCGGATATTGCCGGTCAAAATAAAGCAAATCCACTGGCTG
CTATTCTGGCGGTTGGGATGATGTTGAAACATCTCGGTTATTCTAATCTGGGCGACCGGGTAGAAAAGGCTGTGGAACAG
GCCCTGAAGGAAAATCAGGTAACCCCGGATATGGGAGGAAATTTATCGACTGATGAAGTTGGTAATTATATTGTAACTTT
ATTAAAACAGGACAGGGAAGGTGAGGATGATGAGTAG

Upstream 100 bases:

>100_bases
GAAAAAAATATAAATATTGATTCAACACCCTTTCCCTCTTTTATGCAGGAAATTATTAAAGCAGGTGGGTTAATTGAAAA
AATTAAAGGGGGAATAAAAC

Downstream 100 bases:

>100_bases
TGGGATTAGTCTCACTTTATGATACTACATTAAGGGATGGGGCCCAGGGAGAAGGTATTTCCTTTTCCCTGGATGATAAA
TTAAAAATTGTTTATAGACT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLKIAVIPGDGIGKEVTDQGLKVMEYLNGKNGLQLTLDKFELGAERYLKNGEVLPEKVLKKLETYNSIYLGAVGDPRVPA
GVLEQGILLKLRFYFDQYVNLRPVKLFKDDFSPLKNKGVNDIDFTVIRENTEGLYAGVGGFLKKNTPDEVAIQEMVNTRK
GVDRVIKYAFEYALKYGKEPKVTLCDKSNVLTYAHNLWQRAFKDIAVTYPRVETDHFLVDAITMKMVRNPEIFDVIVTCN
MFGDIITDLGAELQGGMGLAVSGNINPDSVSMFEPVHGSAPDIAGQNKANPLAAILAVGMMLKHLGYSNLGDRVEKAVEQ
ALKENQVTPDMGGNLSTDEVGNYIVTLLKQDREGEDDE

Sequences:

>Translated_358_residues
MLKIAVIPGDGIGKEVTDQGLKVMEYLNGKNGLQLTLDKFELGAERYLKNGEVLPEKVLKKLETYNSIYLGAVGDPRVPA
GVLEQGILLKLRFYFDQYVNLRPVKLFKDDFSPLKNKGVNDIDFTVIRENTEGLYAGVGGFLKKNTPDEVAIQEMVNTRK
GVDRVIKYAFEYALKYGKEPKVTLCDKSNVLTYAHNLWQRAFKDIAVTYPRVETDHFLVDAITMKMVRNPEIFDVIVTCN
MFGDIITDLGAELQGGMGLAVSGNINPDSVSMFEPVHGSAPDIAGQNKANPLAAILAVGMMLKHLGYSNLGDRVEKAVEQ
ALKENQVTPDMGGNLSTDEVGNYIVTLLKQDREGEDDE
>Mature_358_residues
MLKIAVIPGDGIGKEVTDQGLKVMEYLNGKNGLQLTLDKFELGAERYLKNGEVLPEKVLKKLETYNSIYLGAVGDPRVPA
GVLEQGILLKLRFYFDQYVNLRPVKLFKDDFSPLKNKGVNDIDFTVIRENTEGLYAGVGGFLKKNTPDEVAIQEMVNTRK
GVDRVIKYAFEYALKYGKEPKVTLCDKSNVLTYAHNLWQRAFKDIAVTYPRVETDHFLVDAITMKMVRNPEIFDVIVTCN
MFGDIITDLGAELQGGMGLAVSGNINPDSVSMFEPVHGSAPDIAGQNKANPLAAILAVGMMLKHLGYSNLGDRVEKAVEQ
ALKENQVTPDMGGNLSTDEVGNYIVTLLKQDREGEDDE

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=30.5475504322767, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4758582, Length=250, Percent_Identity=32.4, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI28178816, Length=350, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI28178821, Length=349, Percent_Identity=28.9398280802292, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI28178838, Length=238, Percent_Identity=31.5126050420168, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI28178819, Length=185, Percent_Identity=35.1351351351351, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=42.6966292134831, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=35.7541899441341, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=29.2191435768262, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71986051, Length=333, Percent_Identity=31.2312312312312, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=348, Percent_Identity=29.8850574712644, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=361, Percent_Identity=27.1468144044321, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=358, Percent_Identity=29.3296089385475, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=34.733893557423, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=34.5303867403315, Blast_Score=185, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=355, Percent_Identity=33.2394366197183, Blast_Score=164, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=33.7110481586402, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=32.8530259365994, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=32.8530259365994, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24661184, Length=353, Percent_Identity=31.1614730878187, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI161078635, Length=266, Percent_Identity=32.3308270676692, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI161078633, Length=266, Percent_Identity=32.3308270676692, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24650122, Length=266, Percent_Identity=32.3308270676692, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI161078639, Length=266, Percent_Identity=32.3308270676692, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI161078637, Length=266, Percent_Identity=32.3308270676692, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI281362242, Length=263, Percent_Identity=31.9391634980989, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24648872, Length=263, Percent_Identity=31.9391634980989, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI20130355, Length=348, Percent_Identity=22.1264367816092, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39615; Mature: 39615

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIAVIPGDGIGKEVTDQGLKVMEYLNGKNGLQLTLDKFELGAERYLKNGEVLPEKVLK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCHHHHHCCCCCCHHHHHH
KLETYNSIYLGAVGDPRVPAGVLEQGILLKLRFYFDQYVNLRPVKLFKDDFSPLKNKGVN
HHHHHCCEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
DIDFTVIRENTEGLYAGVGGFLKKNTPDEVAIQEMVNTRKGVDRVIKYAFEYALKYGKEP
CCEEEEEEECCCCHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KVTLCDKSNVLTYAHNLWQRAFKDIAVTYPRVETDHFLVDAITMKMVRNPEIFDVIVTCN
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHH
MFGDIITDLGAELQGGMGLAVSGNINPDSVSMFEPVHGSAPDIAGQNKANPLAAILAVGM
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MLKHLGYSNLGDRVEKAVEQALKENQVTPDMGGNLSTDEVGNYIVTLLKQDREGEDDE
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLKIAVIPGDGIGKEVTDQGLKVMEYLNGKNGLQLTLDKFELGAERYLKNGEVLPEKVLK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCHHHHHCCCCCCHHHHHH
KLETYNSIYLGAVGDPRVPAGVLEQGILLKLRFYFDQYVNLRPVKLFKDDFSPLKNKGVN
HHHHHCCEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
DIDFTVIRENTEGLYAGVGGFLKKNTPDEVAIQEMVNTRKGVDRVIKYAFEYALKYGKEP
CCEEEEEEECCCCHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KVTLCDKSNVLTYAHNLWQRAFKDIAVTYPRVETDHFLVDAITMKMVRNPEIFDVIVTCN
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHH
MFGDIITDLGAELQGGMGLAVSGNINPDSVSMFEPVHGSAPDIAGQNKANPLAAILAVGM
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MLKHLGYSNLGDRVEKAVEQALKENQVTPDMGGNLSTDEVGNYIVTLLKQDREGEDDE
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA