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The map label for this gene is leuA

Identifier: 220931927

GI number: 220931927

Start: 1179756

End: 1181303

Strand: Direct

Name: leuA

Synonym: Hore_10860

Alternate gene names: 220931927

Gene position: 1179756-1181303 (Clockwise)

Preceding gene: 220931926

Following gene: 220931928

Centisome position: 45.76

GC content: 38.82

Gene sequence:

>1548_bases
ATGGGTGAGGTTAAAATATTTGATACAACGTTAAGGGATGGCGAGCAGTCTCCCGGCGTCAGTCTTATTCCTGAAGAAAA
ACTTGCTATAGCTAAACAGCTCGCCAGGATGAAAGTTGATGTTATTGAGGCAGGTTTTCCAATTTCTTCACCGGGGGATT
TTGAGGCTGTTCAGATTATCAGCGAGAATATCAGAGATGTTGAGGTGGCTGCCCTGGCCCGGAGCAGGAAAAAGGATATA
GACCGGGCCTGGGAAGCACTAAGAAATGGAGGGGATCCTAGAATTCATGTATTTATTGCTACCTCTCCTATTCATATGAA
ATATAAATTAAAATTGTCTGAGGAACAGGTTATAGAAAAAGCAGTTGAGGCTGTAAAATATGCCTCTAAATACACATCCA
ATATTGAATTTTCTGCTGAGGATGCTTCCCGGAGCCAACCTGTTTTTTTATATCGGCTCTTTGAAAGGGTTATTAATGCG
GGGGCGAAGGTTATTAATATACCTGATACTGTCGGTTATGCGATTCCTGAAGAGTTTGGTAAATTAATAAGAGATATAAA
AGAAAATGTTTCCAATATTGATAAGGTTGATATCAGTGTTCACTGCCATAACGACCTCGGTCTGGCTGTGGCCAATAGTC
TGGCGGCGGTAGAGAATGGAGCCAACCAGATTGAGGTTGCTGTTAATGGTATTGGTGAAAGGGCAGGTAATACAGCACTT
GAAGAGATTATTATGGCCCTTTATACCCGGAAGGATTTTTACAATATCGGGATAAACCAGGATACAACCCAGATTGCGAG
ATTGAGCAAACTGGTTTCAAATTTAACCGGGATGACTATCCAGCCCAATAAAGCTATTGTCGGGGCCAATGCTTTTGCCC
ATGAATCAGGTATTCACCAGGATGGTGTCATTAAAGAAAGAACTACCTATGAAATTATGGATGCCCGGACAATAGGTCTT
AAGGATAATAAACTGGTTCTGGGTAAACATTCAGGACGACATGCCTTTCGGGAGTTTATTCAAAAACTTGGTTATGATAT
AGATGACGAAACTTTTGAAGAGATTTTTATTGAATTTAAGAAACTGGCTGATAAGAAGAAAAATATTACCCATGTTGAAA
TTGAAGCACTTATCGATAATCATTATCATACTTTTGATAAGGTTTATGAACTGGATTACTTATCGGTTAATACCGGTAAC
AAAGTTCTACCGACAGCGACAATAAAGCTTAAAAAGGAGAATAATATCATTGAAAAAGCTGCCTGTAGTGGTGATGGACC
GGTTGATGCTATTTTTCAGGCTATCAATGAAATTGTCGGTATAGATGATATTAAATTAATTTCATACCATATTAATGCTG
TTACTGAAGGTAAGGATGCCCTGGGTGAAGTTATAGTTAAAACTAAAATTGAGGATAACACCTATACTGGACATAGTGCT
ATGACTGATATTACTGAAGCCAGTGCCCTTGCTTATCTCGAAACCATTAATAAATTTTTAACATCAGAACAAACAAGGCA
GACAACTTCTGCACAAGAAGGCATTTAA

Upstream 100 bases:

>100_bases
ATGCCTTAACCAGTAAGGATGAAAATCACCTGATTGAAAAGGTGGGTAAAAAATTAAGGGATATGATGCCCTGGATTAAT
GAATAGAAGGTGTGTTTAAA

Downstream 100 bases:

>100_bases
AAGGAGGTTAAAATAATGGGTATGACTATGATTGAAAAAATAATTGCGGCCCATACCAATAGAGATAAGGTAAAACCAGG
TGATATAGTGAATGCCAGGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MGEVKIFDTTLRDGEQSPGVSLIPEEKLAIAKQLARMKVDVIEAGFPISSPGDFEAVQIISENIRDVEVAALARSRKKDI
DRAWEALRNGGDPRIHVFIATSPIHMKYKLKLSEEQVIEKAVEAVKYASKYTSNIEFSAEDASRSQPVFLYRLFERVINA
GAKVINIPDTVGYAIPEEFGKLIRDIKENVSNIDKVDISVHCHNDLGLAVANSLAAVENGANQIEVAVNGIGERAGNTAL
EEIIMALYTRKDFYNIGINQDTTQIARLSKLVSNLTGMTIQPNKAIVGANAFAHESGIHQDGVIKERTTYEIMDARTIGL
KDNKLVLGKHSGRHAFREFIQKLGYDIDDETFEEIFIEFKKLADKKKNITHVEIEALIDNHYHTFDKVYELDYLSVNTGN
KVLPTATIKLKKENNIIEKAACSGDGPVDAIFQAINEIVGIDDIKLISYHINAVTEGKDALGEVIVKTKIEDNTYTGHSA
MTDITEASALAYLETINKFLTSEQTRQTTSAQEGI

Sequences:

>Translated_515_residues
MGEVKIFDTTLRDGEQSPGVSLIPEEKLAIAKQLARMKVDVIEAGFPISSPGDFEAVQIISENIRDVEVAALARSRKKDI
DRAWEALRNGGDPRIHVFIATSPIHMKYKLKLSEEQVIEKAVEAVKYASKYTSNIEFSAEDASRSQPVFLYRLFERVINA
GAKVINIPDTVGYAIPEEFGKLIRDIKENVSNIDKVDISVHCHNDLGLAVANSLAAVENGANQIEVAVNGIGERAGNTAL
EEIIMALYTRKDFYNIGINQDTTQIARLSKLVSNLTGMTIQPNKAIVGANAFAHESGIHQDGVIKERTTYEIMDARTIGL
KDNKLVLGKHSGRHAFREFIQKLGYDIDDETFEEIFIEFKKLADKKKNITHVEIEALIDNHYHTFDKVYELDYLSVNTGN
KVLPTATIKLKKENNIIEKAACSGDGPVDAIFQAINEIVGIDDIKLISYHINAVTEGKDALGEVIVKTKIEDNTYTGHSA
MTDITEASALAYLETINKFLTSEQTRQTTSAQEGI
>Mature_514_residues
GEVKIFDTTLRDGEQSPGVSLIPEEKLAIAKQLARMKVDVIEAGFPISSPGDFEAVQIISENIRDVEVAALARSRKKDID
RAWEALRNGGDPRIHVFIATSPIHMKYKLKLSEEQVIEKAVEAVKYASKYTSNIEFSAEDASRSQPVFLYRLFERVINAG
AKVINIPDTVGYAIPEEFGKLIRDIKENVSNIDKVDISVHCHNDLGLAVANSLAAVENGANQIEVAVNGIGERAGNTALE
EIIMALYTRKDFYNIGINQDTTQIARLSKLVSNLTGMTIQPNKAIVGANAFAHESGIHQDGVIKERTTYEIMDARTIGLK
DNKLVLGKHSGRHAFREFIQKLGYDIDDETFEEIFIEFKKLADKKKNITHVEIEALIDNHYHTFDKVYELDYLSVNTGNK
VLPTATIKLKKENNIIEKAACSGDGPVDAIFQAINEIVGIDDIKLISYHINAVTEGKDALGEVIVKTKIEDNTYTGHSAM
TDITEASALAYLETINKFLTSEQTRQTTSAQEGI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=48.7903225806452, Blast_Score=441, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320071, Length=360, Percent_Identity=31.1111111111111, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320019, Length=379, Percent_Identity=30.6068601583113, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324682, Length=479, Percent_Identity=26.5135699373695, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=479, Percent_Identity=26.9311064718163, Blast_Score=127, Evalue=5e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_HALOH (B8CX21)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508835.1
- ProteinModelPortal:   B8CX21
- GeneID:   7312828
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_10860
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   CLSK2476424
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57025; Mature: 56894

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVKIFDTTLRDGEQSPGVSLIPEEKLAIAKQLARMKVDVIEAGFPISSPGDFEAVQII
CCCEEEEECHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SENIRDVEVAALARSRKKDIDRAWEALRNGGDPRIHVFIATSPIHMKYKLKLSEEQVIEK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEECCHHHHHHH
AVEAVKYASKYTSNIEFSAEDASRSQPVFLYRLFERVINAGAKVINIPDTVGYAIPEEFG
HHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHCCCCHHHHH
KLIRDIKENVSNIDKVDISVHCHNDLGLAVANSLAAVENGANQIEVAVNGIGERAGNTAL
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHH
EEIIMALYTRKDFYNIGINQDTTQIARLSKLVSNLTGMTIQPNKAIVGANAFAHESGIHQ
HHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEECCHHHHCCCCCC
DGVIKERTTYEIMDARTIGLKDNKLVLGKHSGRHAFREFIQKLGYDIDDETFEEIFIEFK
CCCCCCCCCEEEECCEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KLADKKKNITHVEIEALIDNHYHTFDKVYELDYLSVNTGNKVLPTATIKLKKENNIIEKA
HHHHHHCCCEEEEEEEHHCCCHHHHHHHEEEEEEEECCCCEEECEEEEEEECCCCHHHHH
ACSGDGPVDAIFQAINEIVGIDDIKLISYHINAVTEGKDALGEVIVKTKIEDNTYTGHSA
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHEEEEECCCCCCCCHH
MTDITEASALAYLETINKFLTSEQTRQTTSAQEGI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GEVKIFDTTLRDGEQSPGVSLIPEEKLAIAKQLARMKVDVIEAGFPISSPGDFEAVQII
CCEEEEECHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SENIRDVEVAALARSRKKDIDRAWEALRNGGDPRIHVFIATSPIHMKYKLKLSEEQVIEK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEECCHHHHHHH
AVEAVKYASKYTSNIEFSAEDASRSQPVFLYRLFERVINAGAKVINIPDTVGYAIPEEFG
HHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHCCCCHHHHH
KLIRDIKENVSNIDKVDISVHCHNDLGLAVANSLAAVENGANQIEVAVNGIGERAGNTAL
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHH
EEIIMALYTRKDFYNIGINQDTTQIARLSKLVSNLTGMTIQPNKAIVGANAFAHESGIHQ
HHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEECCHHHHCCCCCC
DGVIKERTTYEIMDARTIGLKDNKLVLGKHSGRHAFREFIQKLGYDIDDETFEEIFIEFK
CCCCCCCCCEEEECCEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KLADKKKNITHVEIEALIDNHYHTFDKVYELDYLSVNTGNKVLPTATIKLKKENNIIEKA
HHHHHHCCCEEEEEEEHHCCCHHHHHHHEEEEEEEECCCCEEECEEEEEEECCCCHHHHH
ACSGDGPVDAIFQAINEIVGIDDIKLISYHINAVTEGKDALGEVIVKTKIEDNTYTGHSA
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHEEEEECCCCCCCCHH
MTDITEASALAYLETINKFLTSEQTRQTTSAQEGI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA