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The map label for this gene is ykvO [H]

Identifier: 220918422

GI number: 220918422

Start: 3703491

End: 3704231

Strand: Direct

Name: ykvO [H]

Synonym: A2cp1_3326

Alternate gene names: 220918422

Gene position: 3703491-3704231 (Clockwise)

Preceding gene: 220918420

Following gene: 220918425

Centisome position: 73.64

GC content: 77.6

Gene sequence:

>741_bases
ATGACGACGACGACTGGGGCCGGGCTGAAGGATCGGACGGTGGTGGTGGTGGGCGGCGGGTCGGGCATCGGCCTCGCGGT
CGCGCGGGAGGCGCTGGCGCGCGGCGCGGAGGTGGTGCTGGCGGGGCGCTCCCGGGAGCGCCTGGCGCGCGCGCTCGGAA
CGCTCGGCGCCGGCGCGCGGGCGCGGGCCGTCGCGGCCGACGTCCGGCAGGAGGACGAGGTGATGCGGCTGTTCCGGGAG
GTCCCGGCCGCCGACCACGTGGTCGCGACGGCGGTCGTGCCCGCGTACCAGCCCATCGCCGCGTTCGACCTCGAGGCGGC
CCGCGCGGTGGTGGACTCGAAGCTGCTCGGCGCGCTGCTGCTCGCGAAGCACGGGGCGCCCAGGCTGCGGCCGGGCGGCT
CGCTCACGTTCACCACCGGGATCGCCGCGGATCGCCCGATGCCACGGGGCGCGATGATCGCCGCCGTGAACGGCGCGCTG
GCCGCCTTCGTGCGGGCCGCGGCGCTGGAGCTCGCCCCGCTGCGGGTGAACGCGCTCTCGCCCGGGTGGGTGGACACGGA
GATCTGGGACGCGGTCGCCGGCGACGGCAAGGCGGCGACGTTCGCCGCGATGGCCGAGCGGCTCCCCGCCCGCCGGATCG
GGCGGCCGGAGGACGTCGCCCACGCCGCCGCGTTCCTGATGGAGAACGGCTTCACCACCGGGAGCGTGCTCCGGGTGGAC
GGGGGGCATCCGCTGGTCTAG

Upstream 100 bases:

>100_bases
AACAGCCGCAGGTCCCCCGGATCGATCATCTCATCCATGGCACGAAGCATACCGGATCGTCCCGTTGATCACCCGCGCCG
CCGCGGGCATGGATGCGTGC

Downstream 100 bases:

>100_bases
GCGGCGCTCCGCGACCCCGTCCTCACGAGGCGAGCGCCCCGGCCGCGCGCGCCGCCGGCGGCCGGGGCGCCCGGCTCACC
CGCGGTCGCGGTCGCGGTAC

Product: short chain dehydrogenase

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTTTTGAGLKDRTVVVVGGGSGIGLAVAREALARGAEVVLAGRSRERLARALGTLGAGARARAVAADVRQEDEVMRLFRE
VPAADHVVATAVVPAYQPIAAFDLEAARAVVDSKLLGALLLAKHGAPRLRPGGSLTFTTGIAADRPMPRGAMIAAVNGAL
AAFVRAAALELAPLRVNALSPGWVDTEIWDAVAGDGKAATFAAMAERLPARRIGRPEDVAHAAAFLMENGFTTGSVLRVD
GGHPLV

Sequences:

>Translated_246_residues
MTTTTGAGLKDRTVVVVGGGSGIGLAVAREALARGAEVVLAGRSRERLARALGTLGAGARARAVAADVRQEDEVMRLFRE
VPAADHVVATAVVPAYQPIAAFDLEAARAVVDSKLLGALLLAKHGAPRLRPGGSLTFTTGIAADRPMPRGAMIAAVNGAL
AAFVRAAALELAPLRVNALSPGWVDTEIWDAVAGDGKAATFAAMAERLPARRIGRPEDVAHAAAFLMENGFTTGSVLRVD
GGHPLV
>Mature_245_residues
TTTTGAGLKDRTVVVVGGGSGIGLAVAREALARGAEVVLAGRSRERLARALGTLGAGARARAVAADVRQEDEVMRLFREV
PAADHVVATAVVPAYQPIAAFDLEAARAVVDSKLLGALLLAKHGAPRLRPGGSLTFTTGIAADRPMPRGAMIAAVNGALA
AFVRAAALELAPLRVNALSPGWVDTEIWDAVAGDGKAATFAAMAERLPARRIGRPEDVAHAAAFLMENGFTTGSVLRVDG
GHPLV

Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=257, Percent_Identity=27.6264591439689, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI32483357, Length=248, Percent_Identity=29.8387096774194, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=29.5081967213115, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=27.8884462151394, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI5031737, Length=232, Percent_Identity=29.3103448275862, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=29.3172690763052, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI10190704, Length=247, Percent_Identity=27.1255060728745, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1790717, Length=242, Percent_Identity=25.2066115702479, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082100, Length=252, Percent_Identity=26.984126984127, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=27.2357723577236, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=246, Percent_Identity=26.0162601626016, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=26.7175572519084, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=28.4615384615385, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=262, Percent_Identity=24.8091603053435, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=245, Percent_Identity=30.2040816326531, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=31.3253012048193, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=30.2788844621514, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355319, Length=243, Percent_Identity=27.5720164609054, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357041, Length=249, Percent_Identity=30.1204819277108, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=240, Percent_Identity=23.75, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644339, Length=246, Percent_Identity=28.4552845528455, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 25206; Mature: 25075

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTGAGLKDRTVVVVGGGSGIGLAVAREALARGAEVVLAGRSRERLARALGTLGAGAR
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCH
ARAVAADVRQEDEVMRLFREVPAADHVVATAVVPAYQPIAAFDLEAARAVVDSKLLGALL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LAKHGAPRLRPGGSLTFTTGIAADRPMPRGAMIAAVNGALAAFVRAAALELAPLRVNALS
HHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEECCC
PGWVDTEIWDAVAGDGKAATFAAMAERLPARRIGRPEDVAHAAAFLMENGFTTGSVLRVD
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEC
GGHPLV
CCCCCC
>Mature Secondary Structure 
TTTTGAGLKDRTVVVVGGGSGIGLAVAREALARGAEVVLAGRSRERLARALGTLGAGAR
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCH
ARAVAADVRQEDEVMRLFREVPAADHVVATAVVPAYQPIAAFDLEAARAVVDSKLLGALL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LAKHGAPRLRPGGSLTFTTGIAADRPMPRGAMIAAVNGALAAFVRAAALELAPLRVNALS
HHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEECCC
PGWVDTEIWDAVAGDGKAATFAAMAERLPARRIGRPEDVAHAAAFLMENGFTTGSVLRVD
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEC
GGHPLV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} 20 {D-gluconate}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]