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The map label for this gene is nadE [H]

Identifier: 220918056

GI number: 220918056

Start: 3268992

End: 3269963

Strand: Reverse

Name: nadE [H]

Synonym: A2cp1_2957

Alternate gene names: 220918056

Gene position: 3269963-3268992 (Counterclockwise)

Preceding gene: 220918059

Following gene: 220918055

Centisome position: 65.02

GC content: 70.99

Gene sequence:

>972_bases
ATGTTCACCAGAGACGTCCTGAAGATCGACGCGCCGCTCGTCTCCGAGCGGATCGAGGGGAGCATCCGCGAGCAGGTCCT
GGGGACCCTGCGTCGCCGTGGCGCGGTCGTCGGGATGTCCGGGGGCATCGACAGCTCGGTGGTCGCCTCGCTCTGCGTCC
GCGCGCTCGGCGCGGATCGGGTGGTGGGCCTGCTCATGCCGGAGCGCGACTCGTCCGGCGACGCGCTGCGGCTGGGCCGC
ATGCTGGCCGCCCACCTCGGCATCCGGCACGTGGTGGAGGACATCGCCCCGGCGCTGGAGGGGCTGGGCTGCTATGCGCG
CCAGCTCGAGGCGATCCGCATGGCGGTGCCGGAGTACGGAGACGGCTGGCGCTGCAAGCTCACGCTCCCCTCGCTCCTGG
AGGGCGAGCGGCTCAACATCACCAGCCTCACCGTGTCGGATCCCCGAGGAACCCAGCGGACCGTGCGCATGCCGCCCGCG
GCGTACCTGCAGATGGTCGCGGCGACGAACTTCAAGCAGCGCACGCGCAAGATGATGGAGTACTACCACGCCGATCGCCT
GAACTACGCGGTGGCGGGGACGCCCAACCGGCTCGAGTACGACCAGGGCTTCTTCGTGAAGCAGGGCGACGGCGCCGCGG
ACTTCAAGCCCATCGCGCACCTCTACAAGACCCAGGTCTACGCGCTGGCCGAGCACCTCGGCGTGCCGGAGGAGATCCGC
CGGCGGCCGCCCACCACCGACACGTTCTCGCTGGCGCAGACGCAGGAGGAGTTCTACTTCGCGCTGCCGTACCGCGAGAT
GGACCTGTGCCTGTGGGCGCACGAGCACGACACGCCGGCCGCCGAGGTGGCGCCGGTGCTGGGCCTCACGCCGGAGCAGG
TCGAGCGCGTCTACCGCGACATCGAGGCGAAGCGCCGGGTGAGCAGGTACCTGCACCAGGCGCCGCTGCTCGTGGCGGAT
CCGCGGGGCTGA

Upstream 100 bases:

>100_bases
GGATCGAGCCTAGAATCCCGACTTCGGCGCCGGGGGGCACCCGTCAACGTGGGACTGCCATGCCGCGCCCCGCAGGCGCG
ATAGTCGCGGTCGGGGGAAC

Downstream 100 bases:

>100_bases
CATGTGCGGCATCGCCGGCATCGTCTCGCTGCGCGACGGGAGCCCGCCGCCGGGGCTCGACGAGCTCCAGGCGATGACCG
GCGCGCTGCGGCACCGCGGC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFTRDVLKIDAPLVSERIEGSIREQVLGTLRRRGAVVGMSGGIDSSVVASLCVRALGADRVVGLLMPERDSSGDALRLGR
MLAAHLGIRHVVEDIAPALEGLGCYARQLEAIRMAVPEYGDGWRCKLTLPSLLEGERLNITSLTVSDPRGTQRTVRMPPA
AYLQMVAATNFKQRTRKMMEYYHADRLNYAVAGTPNRLEYDQGFFVKQGDGAADFKPIAHLYKTQVYALAEHLGVPEEIR
RRPPTTDTFSLAQTQEEFYFALPYREMDLCLWAHEHDTPAAEVAPVLGLTPEQVERVYRDIEAKRRVSRYLHQAPLLVAD
PRG

Sequences:

>Translated_323_residues
MFTRDVLKIDAPLVSERIEGSIREQVLGTLRRRGAVVGMSGGIDSSVVASLCVRALGADRVVGLLMPERDSSGDALRLGR
MLAAHLGIRHVVEDIAPALEGLGCYARQLEAIRMAVPEYGDGWRCKLTLPSLLEGERLNITSLTVSDPRGTQRTVRMPPA
AYLQMVAATNFKQRTRKMMEYYHADRLNYAVAGTPNRLEYDQGFFVKQGDGAADFKPIAHLYKTQVYALAEHLGVPEEIR
RRPPTTDTFSLAQTQEEFYFALPYREMDLCLWAHEHDTPAAEVAPVLGLTPEQVERVYRDIEAKRRVSRYLHQAPLLVAD
PRG
>Mature_323_residues
MFTRDVLKIDAPLVSERIEGSIREQVLGTLRRRGAVVGMSGGIDSSVVASLCVRALGADRVVGLLMPERDSSGDALRLGR
MLAAHLGIRHVVEDIAPALEGLGCYARQLEAIRMAVPEYGDGWRCKLTLPSLLEGERLNITSLTVSDPRGTQRTVRMPPA
AYLQMVAATNFKQRTRKMMEYYHADRLNYAVAGTPNRLEYDQGFFVKQGDGAADFKPIAHLYKTQVYALAEHLGVPEEIR
RRPPTTDTFSLAQTQEEFYFALPYREMDLCLWAHEHDTPAAEVAPVLGLTPEQVERVYRDIEAKRRVSRYLHQAPLLVAD
PRG

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 36104; Mature: 36104

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTRDVLKIDAPLVSERIEGSIREQVLGTLRRRGAVVGMSGGIDSSVVASLCVRALGADR
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCHH
VVGLLMPERDSSGDALRLGRMLAAHLGIRHVVEDIAPALEGLGCYARQLEAIRMAVPEYG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGWRCKLTLPSLLEGERLNITSLTVSDPRGTQRTVRMPPAAYLQMVAATNFKQRTRKMME
CCEEEEEECHHHHCCCCEEEEEEEECCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHH
YYHADRLNYAVAGTPNRLEYDQGFFVKQGDGAADFKPIAHLYKTQVYALAEHLGVPEEIR
HHHHHHCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRPPTTDTFSLAQTQEEFYFALPYREMDLCLWAHEHDTPAAEVAPVLGLTPEQVERVYRD
CCCCCCCCHHHHHHHCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHH
IEAKRRVSRYLHQAPLLVADPRG
HHHHHHHHHHHHHCCEEEECCCC
>Mature Secondary Structure
MFTRDVLKIDAPLVSERIEGSIREQVLGTLRRRGAVVGMSGGIDSSVVASLCVRALGADR
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCHH
VVGLLMPERDSSGDALRLGRMLAAHLGIRHVVEDIAPALEGLGCYARQLEAIRMAVPEYG
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGWRCKLTLPSLLEGERLNITSLTVSDPRGTQRTVRMPPAAYLQMVAATNFKQRTRKMME
CCEEEEEECHHHHCCCCEEEEEEEECCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHH
YYHADRLNYAVAGTPNRLEYDQGFFVKQGDGAADFKPIAHLYKTQVYALAEHLGVPEEIR
HHHHHHCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRPPTTDTFSLAQTQEEFYFALPYREMDLCLWAHEHDTPAAEVAPVLGLTPEQVERVYRD
CCCCCCCCHHHHHHHCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHH
IEAKRRVSRYLHQAPLLVADPRG
HHHHHHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]