Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 220918055

GI number: 220918055

Start: 3266954

End: 3268990

Strand: Reverse

Name: asnB [H]

Synonym: A2cp1_2956

Alternate gene names: 220918055

Gene position: 3268990-3266954 (Counterclockwise)

Preceding gene: 220918056

Following gene: 220918054

Centisome position: 65.0

GC content: 74.47

Gene sequence:

>2037_bases
ATGTGCGGCATCGCCGGCATCGTCTCGCTGCGCGACGGGAGCCCGCCGCCGGGGCTCGACGAGCTCCAGGCGATGACCGG
CGCGCTGCGGCACCGCGGCCCCGACGAGTTCGGCCTGTACCGCGACGCCCGCGCCGGCCTCGGCCACGCGCGCCTCTCGA
TCATCGACCTCGCCACCGGGCAGCAGCCGCTCTCCAACGAGGACGGCACGCTGTGGATCGCGTTCAACGGCGAGATCTTC
AACTACCTGGAGCTGCGCGAGGAGCTCGTCGCGCTCGGGCACCGGTTCCGGACGCGCAGCGACACCGAGGTGATCGTCCA
CGCGTTCGAGGCGTGGGGCGAGGACGCGTTCGCGCGCTTCAACGGCCAGTTCGCGGTGGCGCTGTGGGACTCGGCGCGCC
GGCGGCTGGTGCTGGCGCGCGACCGGGTCGGCGTCCGGCCGCTCTACCTCTGCGAGCACGCCGGTCGGCTGTGGTTCGCC
TCCGAGGTGTGCGCGCTGTTCGCGGGCGCGCCGGACCTCCCGCGCGCGCTGGACCCGGTGGGCCTGGCGGAGACGTTCAC
GTTCTGGACGGTGGTGCCGCCGCAGTCGGTGTTCGCCGGCGTGACCGAGCTCGAGCCCGGCCACGTCCGCACGGTGTCGC
GCGAGGGCACCACCGACCGCGCGTTCTGGGAGCCGTCGTACCCCGCAGGCGAGCGCGGCTTCCGCGGCTCGCTCGACGAG
GCCGTCGAGCGCGTGCGGGCGTCGCTCGAGCAGGCGGTCCGGCTGCGGATGCTCCGGGCCGACGTGCCGGTGGGCAGCTA
CCTCTCCGGCGGGCTGGACAGCTCGCTGGTGGCGGCGCTGGGCCGGCGCGTGAAGGGCGACCGCTTCTGCACGTACTCGA
TCCGCTTCGAGGACGCGGAGTACGACGAGACCGCGTTCCAGCGCGCGGTGGCGGCCCTGATCGAGAGCGATCACCGCGAG
ATCGTCGTCCGCCGCGCCGACATCGCCGAGGCGTTCCCGCAGGTCGTCGCGCACGCCGAGCGGCCCCTGCTCCGGACCGC
GCCGGCGCCGCTGTTCCTGCTCTCGCGGCTGGTGCGCGACTCCGGGATCAAGGTCGTGCTCACCGGCGAGGGCGCCGACG
AGATGTTCGCCGGCTACGACCTCTTCCGCGAGGGCAAGGTGCGCCGGTTCTGGGGCCGGCAGCCCGCGTCGGTGCTCCGC
CCGCGCTTGCTGGAGCGGCTCTACCCGTATCTCGCGCGGTCCCCGGTGGCCCAGCGGGCCATGGCGAGGGAGTTCTTCGG
CAGGGGCCGCGAGGCGCCGGGGGTGCCCGGCTTCGCGCACCAGACCCGCTGGCAGTCGGCGGCGGCGCTCCAGCGGATCT
TCACGCCGGGCGTCCGCGAGGCGGCGCGGTCGGTGGACGTGGTGGCGCGCCTGCTCCGCGATCTGCCGCCTTCGTTCGGC
GCCTGGGCCACGCTCTCGCAGGATCAGTACCTGGAGGTGCGCACGCTGCTCTCGGGGTACCTGCTGTCGTCCCAGGGCGA
CCGGATGCTGATGGCGCACTCGGTGGAGGGGCGCTTCCCGTTCCTCGACGCGGACGTGATCGAGCTCGCGAACGCGCTCC
CGCCCTCGTACAAGCTCCGCGTGCTCGACGAGAAGCACGCGCTGAAGCGCGCCGCGGCAGGGCTGGTGCCGCCCGAGATC
GTGCGCCGGCCCAAGCAGCCCTACCGCGCGCCCGACGCGCTCAGCTTCGTCGGGCCCGGCGCGCCGGGGTGGGCCGCCGA
GCTGATGAGCCCGGAGGGCGTCGCGCGCGCCGGCGTGTTCGAGCCGCGTGCGGTGGACCGGCTCTGGCGGAAGTGCCAGG
CGTCCGCGGGCGGGGAGCAGCTCTCGAACGCGGACAACATGGCGGTGGTGGGCGTGCTGTCCACGGGCCTGCTGCACGAT
CGGCTGGTGCGGAGGGCGCCGGAGCGGGCCGGCCGGCTCCAGCTCACGACCTTGGTGGACCGCCTGGGCGAGCGCCCCGG
CCTTGCGAACGGATTCCAGGAGAGGCAGGCGACATGA

Upstream 100 bases:

>100_bases
GAGCAGGTCGAGCGCGTCTACCGCGACATCGAGGCGAAGCGCCGGGTGAGCAGGTACCTGCACCAGGCGCCGCTGCTCGT
GGCGGATCCGCGGGGCTGAC

Downstream 100 bases:

>100_bases
CGACCCAGCAGATCATCCGGAAGTTCATCCTCGAGAACTTCTACGTCAGCGATCCTTCGGAGGTCGCGGACGACACCTTG
CTCGTGACCACCGGCCTGGT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MCGIAGIVSLRDGSPPPGLDELQAMTGALRHRGPDEFGLYRDARAGLGHARLSIIDLATGQQPLSNEDGTLWIAFNGEIF
NYLELREELVALGHRFRTRSDTEVIVHAFEAWGEDAFARFNGQFAVALWDSARRRLVLARDRVGVRPLYLCEHAGRLWFA
SEVCALFAGAPDLPRALDPVGLAETFTFWTVVPPQSVFAGVTELEPGHVRTVSREGTTDRAFWEPSYPAGERGFRGSLDE
AVERVRASLEQAVRLRMLRADVPVGSYLSGGLDSSLVAALGRRVKGDRFCTYSIRFEDAEYDETAFQRAVAALIESDHRE
IVVRRADIAEAFPQVVAHAERPLLRTAPAPLFLLSRLVRDSGIKVVLTGEGADEMFAGYDLFREGKVRRFWGRQPASVLR
PRLLERLYPYLARSPVAQRAMAREFFGRGREAPGVPGFAHQTRWQSAAALQRIFTPGVREAARSVDVVARLLRDLPPSFG
AWATLSQDQYLEVRTLLSGYLLSSQGDRMLMAHSVEGRFPFLDADVIELANALPPSYKLRVLDEKHALKRAAAGLVPPEI
VRRPKQPYRAPDALSFVGPGAPGWAAELMSPEGVARAGVFEPRAVDRLWRKCQASAGGEQLSNADNMAVVGVLSTGLLHD
RLVRRAPERAGRLQLTTLVDRLGERPGLANGFQERQAT

Sequences:

>Translated_678_residues
MCGIAGIVSLRDGSPPPGLDELQAMTGALRHRGPDEFGLYRDARAGLGHARLSIIDLATGQQPLSNEDGTLWIAFNGEIF
NYLELREELVALGHRFRTRSDTEVIVHAFEAWGEDAFARFNGQFAVALWDSARRRLVLARDRVGVRPLYLCEHAGRLWFA
SEVCALFAGAPDLPRALDPVGLAETFTFWTVVPPQSVFAGVTELEPGHVRTVSREGTTDRAFWEPSYPAGERGFRGSLDE
AVERVRASLEQAVRLRMLRADVPVGSYLSGGLDSSLVAALGRRVKGDRFCTYSIRFEDAEYDETAFQRAVAALIESDHRE
IVVRRADIAEAFPQVVAHAERPLLRTAPAPLFLLSRLVRDSGIKVVLTGEGADEMFAGYDLFREGKVRRFWGRQPASVLR
PRLLERLYPYLARSPVAQRAMAREFFGRGREAPGVPGFAHQTRWQSAAALQRIFTPGVREAARSVDVVARLLRDLPPSFG
AWATLSQDQYLEVRTLLSGYLLSSQGDRMLMAHSVEGRFPFLDADVIELANALPPSYKLRVLDEKHALKRAAAGLVPPEI
VRRPKQPYRAPDALSFVGPGAPGWAAELMSPEGVARAGVFEPRAVDRLWRKCQASAGGEQLSNADNMAVVGVLSTGLLHD
RLVRRAPERAGRLQLTTLVDRLGERPGLANGFQERQAT
>Mature_678_residues
MCGIAGIVSLRDGSPPPGLDELQAMTGALRHRGPDEFGLYRDARAGLGHARLSIIDLATGQQPLSNEDGTLWIAFNGEIF
NYLELREELVALGHRFRTRSDTEVIVHAFEAWGEDAFARFNGQFAVALWDSARRRLVLARDRVGVRPLYLCEHAGRLWFA
SEVCALFAGAPDLPRALDPVGLAETFTFWTVVPPQSVFAGVTELEPGHVRTVSREGTTDRAFWEPSYPAGERGFRGSLDE
AVERVRASLEQAVRLRMLRADVPVGSYLSGGLDSSLVAALGRRVKGDRFCTYSIRFEDAEYDETAFQRAVAALIESDHRE
IVVRRADIAEAFPQVVAHAERPLLRTAPAPLFLLSRLVRDSGIKVVLTGEGADEMFAGYDLFREGKVRRFWGRQPASVLR
PRLLERLYPYLARSPVAQRAMAREFFGRGREAPGVPGFAHQTRWQSAAALQRIFTPGVREAARSVDVVARLLRDLPPSFG
AWATLSQDQYLEVRTLLSGYLLSSQGDRMLMAHSVEGRFPFLDADVIELANALPPSYKLRVLDEKHALKRAAAGLVPPEI
VRRPKQPYRAPDALSFVGPGAPGWAAELMSPEGVARAGVFEPRAVDRLWRKCQASAGGEQLSNADNMAVVGVLSTGLLHD
RLVRRAPERAGRLQLTTLVDRLGERPGLANGFQERQAT

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=403, Percent_Identity=28.7841191066998, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI168229252, Length=403, Percent_Identity=28.7841191066998, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI168229250, Length=403, Percent_Identity=28.7841191066998, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI296010848, Length=375, Percent_Identity=29.3333333333333, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI296010852, Length=304, Percent_Identity=29.6052631578947, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI296010850, Length=304, Percent_Identity=29.6052631578947, Blast_Score=99, Evalue=2e-20,
Organism=Escherichia coli, GI1786889, Length=408, Percent_Identity=29.9019607843137, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71993933, Length=395, Percent_Identity=30.379746835443, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=395, Percent_Identity=30.379746835443, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI25147560, Length=388, Percent_Identity=27.8350515463918, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560178, Length=395, Percent_Identity=28.8607594936709, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=423, Percent_Identity=30.7328605200946, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325403, Length=426, Percent_Identity=29.5774647887324, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553209, Length=404, Percent_Identity=35.1485148514851, Blast_Score=202, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 74854; Mature: 74854

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIVSLRDGSPPPGLDELQAMTGALRHRGPDEFGLYRDARAGLGHARLSIIDLATG
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHCCHHHHHCCCCHHHEEEEEECCC
QQPLSNEDGTLWIAFNGEIFNYLELREELVALGHRFRTRSDTEVIVHAFEAWGEDAFARF
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHC
NGQFAVALWDSARRRLVLARDRVGVRPLYLCEHAGRLWFASEVCALFAGAPDLPRALDPV
CCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
GLAETFTFWTVVPPQSVFAGVTELEPGHVRTVSREGTTDRAFWEPSYPAGERGFRGSLDE
CCHHHEEEEEECCCHHHHCCHHCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCHHH
AVERVRASLEQAVRLRMLRADVPVGSYLSGGLDSSLVAALGRRVKGDRFCTYSIRFEDAE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCC
YDETAFQRAVAALIESDHREIVVRRADIAEAFPQVVAHAERPLLRTAPAPLFLLSRLVRD
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHC
SGIKVVLTGEGADEMFAGYDLFREGKVRRFWGRQPASVLRPRLLERLYPYLARSPVAQRA
CCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
MAREFFGRGREAPGVPGFAHQTRWQSAAALQRIFTPGVREAARSVDVVARLLRDLPPSFG
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
AWATLSQDQYLEVRTLLSGYLLSSQGDRMLMAHSVEGRFPFLDADVIELANALPPSYKLR
CEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEE
VLDEKHALKRAAAGLVPPEIVRRPKQPYRAPDALSFVGPGAPGWAAELMSPEGVARAGVF
EECHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHCCCCCHHHCCCC
EPRAVDRLWRKCQASAGGEQLSNADNMAVVGVLSTGLLHDRLVRRAPERAGRLQLTTLVD
CHHHHHHHHHHHHCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHHCHHHCCCCHHHHHHH
RLGERPGLANGFQERQAT
HHCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MCGIAGIVSLRDGSPPPGLDELQAMTGALRHRGPDEFGLYRDARAGLGHARLSIIDLATG
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHCCHHHHHCCCCHHHEEEEEECCC
QQPLSNEDGTLWIAFNGEIFNYLELREELVALGHRFRTRSDTEVIVHAFEAWGEDAFARF
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHC
NGQFAVALWDSARRRLVLARDRVGVRPLYLCEHAGRLWFASEVCALFAGAPDLPRALDPV
CCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
GLAETFTFWTVVPPQSVFAGVTELEPGHVRTVSREGTTDRAFWEPSYPAGERGFRGSLDE
CCHHHEEEEEECCCHHHHCCHHCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCHHH
AVERVRASLEQAVRLRMLRADVPVGSYLSGGLDSSLVAALGRRVKGDRFCTYSIRFEDAE
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCC
YDETAFQRAVAALIESDHREIVVRRADIAEAFPQVVAHAERPLLRTAPAPLFLLSRLVRD
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHC
SGIKVVLTGEGADEMFAGYDLFREGKVRRFWGRQPASVLRPRLLERLYPYLARSPVAQRA
CCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
MAREFFGRGREAPGVPGFAHQTRWQSAAALQRIFTPGVREAARSVDVVARLLRDLPPSFG
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
AWATLSQDQYLEVRTLLSGYLLSSQGDRMLMAHSVEGRFPFLDADVIELANALPPSYKLR
CEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEE
VLDEKHALKRAAAGLVPPEIVRRPKQPYRAPDALSFVGPGAPGWAAELMSPEGVARAGVF
EECHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHCCCCCHHHCCCC
EPRAVDRLWRKCQASAGGEQLSNADNMAVVGVLSTGLLHDRLVRRAPERAGRLQLTTLVD
CHHHHHHHHHHHHCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHHCHHHCCCCHHHHHHH
RLGERPGLANGFQERQAT
HHCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]