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The map label for this gene is rfaF [H]

Identifier: 220917894

GI number: 220917894

Start: 3088824

End: 3089822

Strand: Reverse

Name: rfaF [H]

Synonym: A2cp1_2795

Alternate gene names: 220917894

Gene position: 3089822-3088824 (Counterclockwise)

Preceding gene: 220917895

Following gene: 220917893

Centisome position: 61.44

GC content: 77.98

Gene sequence:

>999_bases
GTGAGCGATCCCTCCAGCATCCTCGTCATCCGCTACAGCGCGATGGGCGACGTGGTGCTCGCCACCAGCGTGCTGGAGCC
GCTCCGCGCGCGGTTTCCGGGCGCGCGCATCGAGTGGGTGACCGACGCGCTGTACGCGCCGCTGCTGGAGGGGCTGCCCG
GGCTCGCGGCGGTGCACCGGCTGTCGCGCGAGGGCCCGAGCTCCGCGCTGCCGCTGGCGGCGCGGCTGCGCGGGCGGTTC
GACCTGGTCATCGACCTCCAGAACAAGGTGCGGAGCGCGGTGGTGGCCCGCGCCGCGGCCCCGCTGCGCACCGCGTTCAA
GCGGCGCAGCGCGCTCCGGGCGCTCCTGTCGGTGTTCGGCTCGGACCCGCCGCTGGTCCGCGCGCACCAGACCCAGCTCT
ACGGCGAGGCGCTCGCGGCCCTGGGCGTGAGCGGCCCCGGCCCGCTCGAGATCTCGCTCTCGCCGCAGGCGCGCGCGCTC
GCCGCCGACGCGCTGCAGGGCGTGGAGGCGCCGGCGGTCGCGCTCGCCCCCGGCGCGCGCTGGGCCACCAAGCGCTGGCC
CGCCGAGCGGTTCGCGGCGGTCGCCGACGCGCTGCACGCCGAGGGCGTGCGCATCGTCCTGTGCGGCGGCCCCGGCGACC
GCGACGCGTTCGCGGCGTTCCGCGCCGCCACCCGCGCGAAGGTCGCCGCCGATCTGTCTTTCCTGCCGCTCGACGCGCTC
GCCGCCGCCATCGCCCGCGTGCAGCTCCTCATCGCCTGCGACTCGGGCCCGGTGCACCTCGCCACCGCGGTGGGCACGCC
GGTGCTGGCGCTGTTCGGGCCGACCTCGGTCGCGCGCTGGGGGCCGCCGCCCCCCGGGCGCGCGCTCTCGCTCGGCCTCT
CCTGCTCGCCCTGCTCGAACCACGGCGGCGACTACTGCCCCGAGGGCCACCACCGCTGCCTCGCGGACCTGGCGCCGCAG
GCGGTGCTGGCCAGCGCGCGAGAGATGCTGCGGCGATGA

Upstream 100 bases:

>100_bases
GCGTGCTGGCGCGGCGCTCGGTCGGCGCCATCCGCGGCGCGAGCGCGCGCAACGTGGACCACATGCTGTCCATCCTGCCG
CGCGGGAAGGTGGCCGCAGC

Downstream 100 bases:

>100_bases
GCGGGCTCGAGGCGATCTGGTGGCGCGAGCGCCCGGGGCCCCTGGGGGCGCTGGCGGGCGCGCCGCTGCTGCTCGCCGAG
GCGCCGTTCCGCGCCGCCGC

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSDPSSILVIRYSAMGDVVLATSVLEPLRARFPGARIEWVTDALYAPLLEGLPGLAAVHRLSREGPSSALPLAARLRGRF
DLVIDLQNKVRSAVVARAAAPLRTAFKRRSALRALLSVFGSDPPLVRAHQTQLYGEALAALGVSGPGPLEISLSPQARAL
AADALQGVEAPAVALAPGARWATKRWPAERFAAVADALHAEGVRIVLCGGPGDRDAFAAFRAATRAKVAADLSFLPLDAL
AAAIARVQLLIACDSGPVHLATAVGTPVLALFGPTSVARWGPPPPGRALSLGLSCSPCSNHGGDYCPEGHHRCLADLAPQ
AVLASAREMLRR

Sequences:

>Translated_332_residues
MSDPSSILVIRYSAMGDVVLATSVLEPLRARFPGARIEWVTDALYAPLLEGLPGLAAVHRLSREGPSSALPLAARLRGRF
DLVIDLQNKVRSAVVARAAAPLRTAFKRRSALRALLSVFGSDPPLVRAHQTQLYGEALAALGVSGPGPLEISLSPQARAL
AADALQGVEAPAVALAPGARWATKRWPAERFAAVADALHAEGVRIVLCGGPGDRDAFAAFRAATRAKVAADLSFLPLDAL
AAAIARVQLLIACDSGPVHLATAVGTPVLALFGPTSVARWGPPPPGRALSLGLSCSPCSNHGGDYCPEGHHRCLADLAPQ
AVLASAREMLRR
>Mature_331_residues
SDPSSILVIRYSAMGDVVLATSVLEPLRARFPGARIEWVTDALYAPLLEGLPGLAAVHRLSREGPSSALPLAARLRGRFD
LVIDLQNKVRSAVVARAAAPLRTAFKRRSALRALLSVFGSDPPLVRAHQTQLYGEALAALGVSGPGPLEISLSPQARALA
ADALQGVEAPAVALAPGARWATKRWPAERFAAVADALHAEGVRIVLCGGPGDRDAFAAFRAATRAKVAADLSFLPLDALA
AAIARVQLLIACDSGPVHLATAVGTPVLALFGPTSVARWGPPPPGRALSLGLSCSPCSNHGGDYCPEGHHRCLADLAPQA
VLASAREMLRR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=345, Percent_Identity=28.695652173913, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790062, Length=295, Percent_Identity=28.8135593220339, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 34592; Mature: 34461

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPSSILVIRYSAMGDVVLATSVLEPLRARFPGARIEWVTDALYAPLLEGLPGLAAVHR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
LSREGPSSALPLAARLRGRFDLVIDLQNKVRSAVVARAAAPLRTAFKRRSALRALLSVFG
HHCCCCCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SDPPLVRAHQTQLYGEALAALGVSGPGPLEISLSPQARALAADALQGVEAPAVALAPGAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEECCCCC
WATKRWPAERFAAVADALHAEGVRIVLCGGPGDRDAFAAFRAATRAKVAADLSFLPLDAL
HHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
AAAIARVQLLIACDSGPVHLATAVGTPVLALFGPTSVARWGPPPPGRALSLGLSCSPCSN
HHHHHHHEEEEEECCCCEEEEHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCC
HGGDYCPEGHHRCLADLAPQAVLASAREMLRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDPSSILVIRYSAMGDVVLATSVLEPLRARFPGARIEWVTDALYAPLLEGLPGLAAVHR
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
LSREGPSSALPLAARLRGRFDLVIDLQNKVRSAVVARAAAPLRTAFKRRSALRALLSVFG
HHCCCCCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SDPPLVRAHQTQLYGEALAALGVSGPGPLEISLSPQARALAADALQGVEAPAVALAPGAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEECCCCC
WATKRWPAERFAAVADALHAEGVRIVLCGGPGDRDAFAAFRAATRAKVAADLSFLPLDAL
HHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
AAAIARVQLLIACDSGPVHLATAVGTPVLALFGPTSVARWGPPPPGRALSLGLSCSPCSN
HHHHHHHEEEEEECCCCEEEEHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCC
HGGDYCPEGHHRCLADLAPQAVLASAREMLRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]