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The map label for this gene is waaA [H]

Identifier: 220917895

GI number: 220917895

Start: 3089819

End: 3091108

Strand: Reverse

Name: waaA [H]

Synonym: A2cp1_2796

Alternate gene names: 220917895

Gene position: 3091108-3089819 (Counterclockwise)

Preceding gene: 220917896

Following gene: 220917894

Centisome position: 61.46

GC content: 73.33

Gene sequence:

>1290_bases
GTGCACCTCGTCTACGCCATCGCCACCTACCTGCTGTTCGTGATCGGGCTGCCGTTCCTGCTCACGCACCCCAAGCTGCG
CCACGGGATCGCCCAGCGGCTCGGGCTGTACGGGCGCTCGCTGGACCGGGGCCGCGGCAGCCCGCGCATCTGGCTGCACG
GGGCGAGCGCCGGGGACCTGCTCTCGCTCCAGCCCATGATGGCGGAGCTGAAGGCCCGCATGCCGGGCTGCTGCGTGATC
GTCAGCACCATGACGAACAGCGGGCTCGCCATGGCCCGCAAGAAGCTGGGCGGGGTGGCGGACGTGGTGGTGTACGCACC
GTACGACCTCCCCGGCGCCACCCGGCGGGCGGTGCGGGCGCTCGAGCCGGACCTGCTCGTGCTCGAGTACACCGAGATCT
GGCCGAACCTGATCCGCTCCGCCCACAAGGCGGGCGTGCGCATCGCGCTCACCAACGGCCGCTTCAACCCGGAGAACCTC
TCGCGCTACCGCGCGCTGTTCCTCGCCATCGGCAACCCGCTGCGCCGCGTCGACTGCTTCCTGATGCGCTCCGACGAGGA
GGCCGAGCGCGTGCTCGCGCTCGGCGCGGCGCCGGACCGGGTCTGGGTGACGGGCAACACCAAGTTCGACGCGCTGGTGC
TGGACGCGGCCGAGGGCGGCAAGGCCGAGGCGCTGCGCGCGGAGATGGGGTTGCAGGCGGGCGCGCCGGTGCTCATGGCC
GGCTCCACGCACGAGGGCGAGGAGGAGCTGATCCTGGGCGCGTACCGCAAGCTGCTGGCGCGCCACCCTCGCCTGCAGCT
CGTCGTCGCGCCGCGCTACGTGGAGCGCTCCGGGCGCATCATGGCGCTCGCCGCCGAGGCGGGGCTCTCGGTGCGCCTGC
GCAGCGGCGGCGCGGCGGCGGGGCACGCGCAGGTCACCGTGCTCGACACGATCGGCGAGCTGGCGGCCGCGTACGGGCTG
GCCACGCTCGTGTTCGTGGGCGGGAGCTTCGTCACCCGCGGCGGCCAGAACGTGCTCGAGCCGGCCGCGCAGGGGCGCCC
GGTGCTGTTCGGGCCGCACATGGAGAACTTCAAGGACTCGGTCCAGGTGCTGCAGGGGAGGGGAGGGATCCAGGTCGCGA
CGCCCGAGAAGCTGCTCAAGGTGGCGGACGAGCTGCTCTCGCGACCCGATCAGCTCCAGGAGCTGGGCGTGCTGGCGCGG
CGCTCGGTCGGCGCCATCCGCGGCGCGAGCGCGCGCAACGTGGACCACATGCTGTCCATCCTGCCGCGCGGGAAGGTGGC
CGCAGCGTGA

Upstream 100 bases:

>100_bases
GGACGGTGCCCGCCGGGCGACTACCGCGCCGAGGTCGCGCTCGAAGGGCGTCGCTGGATCTTCACGAACCCGGTCCGCAT
CGAGTAAGAAGCGGCCAGCC

Downstream 100 bases:

>100_bases
GCGATCCCTCCAGCATCCTCGTCATCCGCTACAGCGCGATGGGCGACGTGGTGCTCGCCACCAGCGTGCTGGAGCCGCTC
CGCGCGCGGTTTCCGGGCGC

Product: Three-deoxy-D-manno-octulosonic-acid transferase domain-containing protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MHLVYAIATYLLFVIGLPFLLTHPKLRHGIAQRLGLYGRSLDRGRGSPRIWLHGASAGDLLSLQPMMAELKARMPGCCVI
VSTMTNSGLAMARKKLGGVADVVVYAPYDLPGATRRAVRALEPDLLVLEYTEIWPNLIRSAHKAGVRIALTNGRFNPENL
SRYRALFLAIGNPLRRVDCFLMRSDEEAERVLALGAAPDRVWVTGNTKFDALVLDAAEGGKAEALRAEMGLQAGAPVLMA
GSTHEGEEELILGAYRKLLARHPRLQLVVAPRYVERSGRIMALAAEAGLSVRLRSGGAAAGHAQVTVLDTIGELAAAYGL
ATLVFVGGSFVTRGGQNVLEPAAQGRPVLFGPHMENFKDSVQVLQGRGGIQVATPEKLLKVADELLSRPDQLQELGVLAR
RSVGAIRGASARNVDHMLSILPRGKVAAA

Sequences:

>Translated_429_residues
MHLVYAIATYLLFVIGLPFLLTHPKLRHGIAQRLGLYGRSLDRGRGSPRIWLHGASAGDLLSLQPMMAELKARMPGCCVI
VSTMTNSGLAMARKKLGGVADVVVYAPYDLPGATRRAVRALEPDLLVLEYTEIWPNLIRSAHKAGVRIALTNGRFNPENL
SRYRALFLAIGNPLRRVDCFLMRSDEEAERVLALGAAPDRVWVTGNTKFDALVLDAAEGGKAEALRAEMGLQAGAPVLMA
GSTHEGEEELILGAYRKLLARHPRLQLVVAPRYVERSGRIMALAAEAGLSVRLRSGGAAAGHAQVTVLDTIGELAAAYGL
ATLVFVGGSFVTRGGQNVLEPAAQGRPVLFGPHMENFKDSVQVLQGRGGIQVATPEKLLKVADELLSRPDQLQELGVLAR
RSVGAIRGASARNVDHMLSILPRGKVAAA
>Mature_429_residues
MHLVYAIATYLLFVIGLPFLLTHPKLRHGIAQRLGLYGRSLDRGRGSPRIWLHGASAGDLLSLQPMMAELKARMPGCCVI
VSTMTNSGLAMARKKLGGVADVVVYAPYDLPGATRRAVRALEPDLLVLEYTEIWPNLIRSAHKAGVRIALTNGRFNPENL
SRYRALFLAIGNPLRRVDCFLMRSDEEAERVLALGAAPDRVWVTGNTKFDALVLDAAEGGKAEALRAEMGLQAGAPVLMA
GSTHEGEEELILGAYRKLLARHPRLQLVVAPRYVERSGRIMALAAEAGLSVRLRSGGAAAGHAQVTVLDTIGELAAAYGL
ATLVFVGGSFVTRGGQNVLEPAAQGRPVLFGPHMENFKDSVQVLQGRGGIQVATPEKLLKVADELLSRPDQLQELGVLAR
RSVGAIRGASARNVDHMLSILPRGKVAAA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=370, Percent_Identity=38.3783783783784, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46009; Mature: 46009

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLVYAIATYLLFVIGLPFLLTHPKLRHGIAQRLGLYGRSLDRGRGSPRIWLHGASAGDL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCC
LSLQPMMAELKARMPGCCVIVSTMTNSGLAMARKKLGGVADVVVYAPYDLPGATRRAVRA
EEHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LEPDLLVLEYTEIWPNLIRSAHKAGVRIALTNGRFNPENLSRYRALFLAIGNPLRRVDCF
CCCCEEEEEHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEE
LMRSDEEAERVLALGAAPDRVWVTGNTKFDALVLDAAEGGKAEALRAEMGLQAGAPVLMA
EECCCHHHHHEEEECCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEE
GSTHEGEEELILGAYRKLLARHPRLQLVVAPRYVERSGRIMALAAEAGLSVRLRSGGAAA
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCEEEEEECCCCEEEEECCCCCC
GHAQVTVLDTIGELAAAYGLATLVFVGGSFVTRGGQNVLEPAAQGRPVLFGPHMENFKDS
CCEEEEEHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHCCCCEEECCCHHHHHHH
VQVLQGRGGIQVATPEKLLKVADELLSRPDQLQELGVLARRSVGAIRGASARNVDHMLSI
HHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LPRGKVAAA
CCCCCCCCC
>Mature Secondary Structure
MHLVYAIATYLLFVIGLPFLLTHPKLRHGIAQRLGLYGRSLDRGRGSPRIWLHGASAGDL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCC
LSLQPMMAELKARMPGCCVIVSTMTNSGLAMARKKLGGVADVVVYAPYDLPGATRRAVRA
EEHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LEPDLLVLEYTEIWPNLIRSAHKAGVRIALTNGRFNPENLSRYRALFLAIGNPLRRVDCF
CCCCEEEEEHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEE
LMRSDEEAERVLALGAAPDRVWVTGNTKFDALVLDAAEGGKAEALRAEMGLQAGAPVLMA
EECCCHHHHHEEEECCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEE
GSTHEGEEELILGAYRKLLARHPRLQLVVAPRYVERSGRIMALAAEAGLSVRLRSGGAAA
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCEEEEEECCCCEEEEECCCCCC
GHAQVTVLDTIGELAAAYGLATLVFVGGSFVTRGGQNVLEPAAQGRPVLFGPHMENFKDS
CCEEEEEHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHCCCCEEECCCHHHHHHH
VQVLQGRGGIQVATPEKLLKVADELLSRPDQLQELGVLARRSVGAIRGASARNVDHMLSI
HHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LPRGKVAAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]