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The map label for this gene is gcvH

Identifier: 220917814

GI number: 220917814

Start: 2994422

End: 2994808

Strand: Direct

Name: gcvH

Synonym: A2cp1_2715

Alternate gene names: 220917814

Gene position: 2994422-2994808 (Clockwise)

Preceding gene: 220917813

Following gene: 220917815

Centisome position: 59.54

GC content: 66.41

Gene sequence:

>387_bases
ATGGAAATCCCCGAGGACCTCAGGTACACGCGCGAGCACGAGTGGGCGCGCCGCAAGGGCAGCGCGATCGTGGTCGGCAT
CACCGACTTCGCCCAGCAGCAGCTCGGCGACGTCGTCTACGTCGAGCTCCCGGACGTCGGCGACCCCGTCAAGAAGGGCG
AGTCGTTCGGCGTGGTCGAGTCCACCAAGGCCGTCTCCGAGCTGTTCGCCCCGATCTCCGGCAAGGTGATCGAGGTGAAC
GACCCGCTCTCCGACGCGCCCGAGACCATCAACGAGGATCCGTACGAGGAGGGCTGGATGATCACCATCGAGCCGTCCGA
TCCGAAGGACCTCGAGGCGCTGATGGACGCCGCCGCCTACAAGGCGTTCGTCGAGGAGCAGGAGTAG

Upstream 100 bases:

>100_bases
CCGCCGAGGGCTCCACCTTCGCAGTCGAGATCCGCGGCCGGGCCGCGGCGGCCAAGGCCGTGAAGACGCCGTTCTACACT
CGGAAGTGACGGAGACGACC

Downstream 100 bases:

>100_bases
CGCCGAGCGCCGCCCGCCGGGCGGTCCCCCTCCCTCGCGGCGCCGTCGCCGCGGAGCCAAGCCTTGCGATACCACCCGCA
CACCCCGGACGACGTCCGCG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MEIPEDLRYTREHEWARRKGSAIVVGITDFAQQQLGDVVYVELPDVGDPVKKGESFGVVESTKAVSELFAPISGKVIEVN
DPLSDAPETINEDPYEEGWMITIEPSDPKDLEALMDAAAYKAFVEEQE

Sequences:

>Translated_128_residues
MEIPEDLRYTREHEWARRKGSAIVVGITDFAQQQLGDVVYVELPDVGDPVKKGESFGVVESTKAVSELFAPISGKVIEVN
DPLSDAPETINEDPYEEGWMITIEPSDPKDLEALMDAAAYKAFVEEQE
>Mature_128_residues
MEIPEDLRYTREHEWARRKGSAIVVGITDFAQQQLGDVVYVELPDVGDPVKKGESFGVVESTKAVSELFAPISGKVIEVN
DPLSDAPETINEDPYEEGWMITIEPSDPKDLEALMDAAAYKAFVEEQE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=42.9752066115703, Blast_Score=119, Evalue=6e-28,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=42.1487603305785, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=47.6190476190476, Blast_Score=136, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=46.6666666666667, Blast_Score=119, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=44.1666666666667, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=41.3223140495868, Blast_Score=100, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=44.7154471544715, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_ANAD2 (B8JDY7)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493118.1
- ProteinModelPortal:   B8JDY7
- SMR:   B8JDY7
- GeneID:   7299716
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2715
- HOGENOM:   HBG693789
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14229; Mature: 14229

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPEDLRYTREHEWARRKGSAIVVGITDFAQQQLGDVVYVELPDVGDPVKKGESFGVVE
CCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCEEEEECCCCCCHHHCCCCCCCHH
STKAVSELFAPISGKVIEVNDPLSDAPETINEDPYEEGWMITIEPSDPKDLEALMDAAAY
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
KAFVEEQE
HHHHHCCC
>Mature Secondary Structure
MEIPEDLRYTREHEWARRKGSAIVVGITDFAQQQLGDVVYVELPDVGDPVKKGESFGVVE
CCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCEEEEECCCCCCHHHCCCCCCCHH
STKAVSELFAPISGKVIEVNDPLSDAPETINEDPYEEGWMITIEPSDPKDLEALMDAAAY
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
KAFVEEQE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA