Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 220917524

GI number: 220917524

Start: 2682111

End: 2683301

Strand: Reverse

Name: pgk

Synonym: A2cp1_2424

Alternate gene names: 220917524

Gene position: 2683301-2682111 (Counterclockwise)

Preceding gene: 220917525

Following gene: 220917523

Centisome position: 53.35

GC content: 70.78

Gene sequence:

>1191_bases
ATGGCGCTCAGGACCATCGACGCCCTCGAGCTCGCGGGCAAGCGGGTCTTCATCCGCGTGGACTTCAACGTCCCGCTCGA
CCCGCAGGGCAGGGTGACCGACGACGCGCGCATCCGCGCCGCGCTGCCGACCATCCGCCACGCGATCCAGGCCAAGGCCA
AGGTGATCCTGGCCTCGCACCTGGGGCGCCCGAAGGGCAAGCCGGAGGATCGCCAGAAGCTCACCCTGGAGCCGGCGGCG
GTGCGGCTCTCCGAACTGCTCTCCCAGGACGTGATCCTGGCGGACGACTGCGTCGGCGACGGGGTGAAGAAGCTGGTGCG
GGACCTGAAGGACGGGCACGTCCTCCTGCTCGAGAACCTCCGGTTCCACCCCGAGGAGGAGAAGAACGACGAGGCGTTCG
CCCGCGAGCTGGCCAGCCTGGCCGACGTGTGGGTGAACGACGCGTTCGGCACCGCGCACCGCGCCCACGCCTCGACCGCG
GGCATGGCGAGGTTCGTGAAGGAGAAGGCCGCCGGCTTCCTGGTGCAGAAGGAGGTCGAGTACCTGGGCAAGGCGCTCGG
CAGCCCGGCGCGGCCGTTCGTCGCCATCGTCGGCGGCGCCAAGGTGTCGGACAAGATCAAGGTCCTCGAGAACCTCATCG
CCAAGGCCGACGCGATCTGCGTCGGCGGCGCGATGGCCTACACGTTCCTGAAGGCGCAGGGCGTGCCGGTGGGGAAGAGC
CTGGTCGAGGAGGACAAGCTCGAGCTGGCCCGCCAGATCCTGGAGCGCGCCGAGGCCCGCAAGGTGGACTTGCTGCTCCC
GGTGGACCACGTCTGCGGCGCGGAGCCCAAGGAGACCGCCGAGCGCGTGGTCGTGAACGACCGCGCCATCCCCGACGGCC
TCATGGGCCTCGACATCGGCCCGAAGACGCTCGACCGCTACCGGCAGCGCATCGCCGCGGCGAAGACCGTCTTCTGGAAC
GGCCCCATGGGCCTGTTCGAGCAGAAGCCGTGGTCCGAGGGCACCTTCGGCGTCGCGAAGGCGATGGCGGCGAGCCCGGC
CGTCACCGTGGTGGGCGGCGGCGACAGCGCCGCGGCCGTCGAGCAGGCCGGTCTCGTGGACGCGATGAAGCACGTCTCCA
CCGGCGGCGGCGCCAGCCTCGAGTTCATCGAGGGGCGAGAGCTGCCCGGCGTGAAGGCTTGCGAGGAGTGA

Upstream 100 bases:

>100_bases
ACGGCAACTTCGCGAAGGTGTTCGCCTGGTACGACAACGAGTGGGGCTTCTCGAACCGCATGGTGGACGTGGCGAAGCTC
GTCACGAAGTGAGAGCCGCC

Downstream 100 bases:

>100_bases
CCGTGGCCCGCACCAAGTTCGTCTGCGGCAACTGGAAGATGCACAAGACGGTCGCCGAGGCGGTCCAGCTCGTGAAGGAG
CTGGCCGCCGGGCTCGGCGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MALRTIDALELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPEDRQKLTLEPAA
VRLSELLSQDVILADDCVGDGVKKLVRDLKDGHVLLLENLRFHPEEEKNDEAFARELASLADVWVNDAFGTAHRAHASTA
GMARFVKEKAAGFLVQKEVEYLGKALGSPARPFVAIVGGAKVSDKIKVLENLIAKADAICVGGAMAYTFLKAQGVPVGKS
LVEEDKLELARQILERAEARKVDLLLPVDHVCGAEPKETAERVVVNDRAIPDGLMGLDIGPKTLDRYRQRIAAAKTVFWN
GPMGLFEQKPWSEGTFGVAKAMAASPAVTVVGGGDSAAAVEQAGLVDAMKHVSTGGGASLEFIEGRELPGVKACEE

Sequences:

>Translated_396_residues
MALRTIDALELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPEDRQKLTLEPAA
VRLSELLSQDVILADDCVGDGVKKLVRDLKDGHVLLLENLRFHPEEEKNDEAFARELASLADVWVNDAFGTAHRAHASTA
GMARFVKEKAAGFLVQKEVEYLGKALGSPARPFVAIVGGAKVSDKIKVLENLIAKADAICVGGAMAYTFLKAQGVPVGKS
LVEEDKLELARQILERAEARKVDLLLPVDHVCGAEPKETAERVVVNDRAIPDGLMGLDIGPKTLDRYRQRIAAAKTVFWN
GPMGLFEQKPWSEGTFGVAKAMAASPAVTVVGGGDSAAAVEQAGLVDAMKHVSTGGGASLEFIEGRELPGVKACEE
>Mature_395_residues
ALRTIDALELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPEDRQKLTLEPAAV
RLSELLSQDVILADDCVGDGVKKLVRDLKDGHVLLLENLRFHPEEEKNDEAFARELASLADVWVNDAFGTAHRAHASTAG
MARFVKEKAAGFLVQKEVEYLGKALGSPARPFVAIVGGAKVSDKIKVLENLIAKADAICVGGAMAYTFLKAQGVPVGKSL
VEEDKLELARQILERAEARKVDLLLPVDHVCGAEPKETAERVVVNDRAIPDGLMGLDIGPKTLDRYRQRIAAAKTVFWNG
PMGLFEQKPWSEGTFGVAKAMAASPAVTVVGGGDSAAAVEQAGLVDAMKHVSTGGGASLEFIEGRELPGVKACEE

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=43.9320388349515, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=43.5523114355231, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=45.4773869346734, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=49.2682926829268, Blast_Score=363, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=45.5205811138014, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24581203, Length=418, Percent_Identity=44.7368421052632, Blast_Score=335, Evalue=2e-92,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=46.3592233009709, Blast_Score=334, Evalue=7e-92,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ANAD2 (B8JBD5)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492828.1
- ProteinModelPortal:   B8JBD5
- SMR:   B8JBD5
- GeneID:   7300084
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2424
- HOGENOM:   HBG453500
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42463; Mature: 42332

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 121-121 BINDING 154-154 BINDING 205-205 BINDING 296-296 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRTIDALELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASH
CCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGRPKGKPEDRQKLTLEPAAVRLSELLSQDVILADDCVGDGVKKLVRDLKDGHVLLLENL
CCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEHHHHCHHHHHHHHHCCCCCEEEEECC
RFHPEEEKNDEAFARELASLADVWVNDAFGTAHRAHASTAGMARFVKEKAAGFLVQKEVE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLGKALGSPARPFVAIVGGAKVSDKIKVLENLIAKADAICVGGAMAYTFLKAQGVPVGKS
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCCCCCHH
LVEEDKLELARQILERAEARKVDLLLPVDHVCGAEPKETAERVVVNDRAIPDGLMGLDIG
HHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCHHHHHHHEEECCCCCCCCCCCCCCC
PKTLDRYRQRIAAAKTVFWNGPMGLFEQKPWSEGTFGVAKAMAASPAVTVVGGGDSAAAV
HHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHH
EQAGLVDAMKHVSTGGGASLEFIEGRELPGVKACEE
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ALRTIDALELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASH
CCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGRPKGKPEDRQKLTLEPAAVRLSELLSQDVILADDCVGDGVKKLVRDLKDGHVLLLENL
CCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEHHHHCHHHHHHHHHCCCCCEEEEECC
RFHPEEEKNDEAFARELASLADVWVNDAFGTAHRAHASTAGMARFVKEKAAGFLVQKEVE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLGKALGSPARPFVAIVGGAKVSDKIKVLENLIAKADAICVGGAMAYTFLKAQGVPVGKS
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCCCCCHH
LVEEDKLELARQILERAEARKVDLLLPVDHVCGAEPKETAERVVVNDRAIPDGLMGLDIG
HHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCHHHHHHHEEECCCCCCCCCCCCCCC
PKTLDRYRQRIAAAKTVFWNGPMGLFEQKPWSEGTFGVAKAMAASPAVTVVGGGDSAAAV
HHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHH
EQAGLVDAMKHVSTGGGASLEFIEGRELPGVKACEE
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA