Click here to switch to the map view.

The map label for this gene is leuC

Identifier: 220917086

GI number: 220917086

Start: 2239664

End: 2241106

Strand: Direct

Name: leuC

Synonym: A2cp1_1983

Alternate gene names: 220917086

Gene position: 2239664-2241106 (Clockwise)

Preceding gene: 220917085

Following gene: 220917087

Centisome position: 44.53

GC content: 74.71

Gene sequence:

>1443_bases
ATGTCGAAGCGCAGCGACGGCAGCCAGCCGAGGACCATCGTCGACAAGGTCTGGGACGCGCACGTGGTCCGCGCGGAGAC
CCCGGACGCGCCCGCGATCCTCTACATCGACCTGCACCTCGTGCACGAGGTGACGAGCCCGCAGGCGTTCACGGTGCTCC
GGGAGCGCGGCCTGAAGCTCCGCCGCCCCGAGCGCACGCTCGCCACCATGGACCACTCCATCCCGACGCTGCCCCGCGGC
GCGGATGGGCGGTGGCCGTTCGTGGACGCGCAGGCGGCGGCGCAGGTCTCGCAGATGGAGCGCAACTGCGCCGACTTCGG
CGTCGAGCTTCACGGGCTCGGCGACGACGCGCAGGGCGTGGTCCACGTGTTCGGCCCGGAGATGGGCGCCACCCAGCCCG
GCATGACGGTGGTGTGCGGCGACAGCCACACCGCCACGCACGGCGCGTTCGGCGCCCTCGCGTTCGGCATCGGCACCTCC
GAGGTCGGCCACGTGCTCGCCTCGCAGTGCCTGCTGCAGCGCCGCCCCCGCACGCTGGCGGTGCGGGTGGACGGGGAGCT
CGGCCCCGGCCTCTCCGCCAAGGACCTGATCCTCGCGATCATCGCGAAGCTCGGGGTCGGCGGCGGCACCGGCCACGTCA
TCGAGTATCTCGGCCCCGCGGTCCGCGCGCTCTCGATGGAGGGCCGCATGACCCTCTGCAACATGTCGATCGAGGCGGGC
GCCCGCGCCGGCCTGGTCGCGCCGGACGACACCACCTTCGAGTGGCTGGCCGGCCGGCCCCGCGCGCCGAAGGGCGCCGC
GTGGGACGAGGCGGTGGCCCGCTGGCGCGCGCTGCCGTCGGACGACGGCGCCACCTACGATCGCGAGCTCCGCCTGGACG
CCGCCGCGCTCGAGCCGATGATCACGTTCGGCACGAACCCGGGGCAGGGCATCGCCGTCACCGGCCTGGTGCCGGACCCG
GTCGCCGAGCGGGACGCCTCCGCGCGCGCCACCCTCGAGGCCGCGCTCCGCTACATGGGGCTCGTCCCCGGCAAGCCCAT
CGCCGGCCAGAAGGTGGACGTGGTGTTCATCGGCTCCTGCACGAACGGCCGCATCGAGGACATGCGCGAGGCGGCCCGGG
TGCTGAAGGGGCGCAAGGTGCGGACCCGCACGCTGGTGGTGCCCGGCTCGCACCGGGTGAAGAAGGACGCCGAGGCCGAG
GGCATCGACCGCATCGTGCGCGAGGCCGGCGCCGAGTGGCGCGAGCCGGGCTGCTCGATGTGCATCGCCATGAACGGCGA
CAACCTCCAGGCCGGCCAGTACTGCGTCTCGACGTCGAACCGCAACTTCGAGGGGCGCCAGGGCCCCGGCGGGCGGACGC
TCCTCGCCAGCCCGCTCACCGCCGCGGCCGCCGCGGTGACGGGCGCCGTCGCCGATCCCCGCCGCGTGCTGGAGGGCCGG
TGA

Upstream 100 bases:

>100_bases
AGGGCAGCGCGCGGGCGTTCCTCGACGTGGTCAACCGGATCGCCCTGAAGATGCCACCGCCCACCGAGAGCGAGGCCCGC
GCGGAGATGGGGACGCCCTG

Downstream 100 bases:

>100_bases
TGGAACCGATCCGCGTCATCGAGAGCCGCACCGTCGTCCTCCCCCGCGAGAACGTCGACACCGACCAGATCATCCCGGCC
CGCTTCCTCAAGGTCACCGA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSKRSDGSQPRTIVDKVWDAHVVRAETPDAPAILYIDLHLVHEVTSPQAFTVLRERGLKLRRPERTLATMDHSIPTLPRG
ADGRWPFVDAQAAAQVSQMERNCADFGVELHGLGDDAQGVVHVFGPEMGATQPGMTVVCGDSHTATHGAFGALAFGIGTS
EVGHVLASQCLLQRRPRTLAVRVDGELGPGLSAKDLILAIIAKLGVGGGTGHVIEYLGPAVRALSMEGRMTLCNMSIEAG
ARAGLVAPDDTTFEWLAGRPRAPKGAAWDEAVARWRALPSDDGATYDRELRLDAAALEPMITFGTNPGQGIAVTGLVPDP
VAERDASARATLEAALRYMGLVPGKPIAGQKVDVVFIGSCTNGRIEDMREAARVLKGRKVRTRTLVVPGSHRVKKDAEAE
GIDRIVREAGAEWREPGCSMCIAMNGDNLQAGQYCVSTSNRNFEGRQGPGGRTLLASPLTAAAAAVTGAVADPRRVLEGR

Sequences:

>Translated_480_residues
MSKRSDGSQPRTIVDKVWDAHVVRAETPDAPAILYIDLHLVHEVTSPQAFTVLRERGLKLRRPERTLATMDHSIPTLPRG
ADGRWPFVDAQAAAQVSQMERNCADFGVELHGLGDDAQGVVHVFGPEMGATQPGMTVVCGDSHTATHGAFGALAFGIGTS
EVGHVLASQCLLQRRPRTLAVRVDGELGPGLSAKDLILAIIAKLGVGGGTGHVIEYLGPAVRALSMEGRMTLCNMSIEAG
ARAGLVAPDDTTFEWLAGRPRAPKGAAWDEAVARWRALPSDDGATYDRELRLDAAALEPMITFGTNPGQGIAVTGLVPDP
VAERDASARATLEAALRYMGLVPGKPIAGQKVDVVFIGSCTNGRIEDMREAARVLKGRKVRTRTLVVPGSHRVKKDAEAE
GIDRIVREAGAEWREPGCSMCIAMNGDNLQAGQYCVSTSNRNFEGRQGPGGRTLLASPLTAAAAAVTGAVADPRRVLEGR
>Mature_479_residues
SKRSDGSQPRTIVDKVWDAHVVRAETPDAPAILYIDLHLVHEVTSPQAFTVLRERGLKLRRPERTLATMDHSIPTLPRGA
DGRWPFVDAQAAAQVSQMERNCADFGVELHGLGDDAQGVVHVFGPEMGATQPGMTVVCGDSHTATHGAFGALAFGIGTSE
VGHVLASQCLLQRRPRTLAVRVDGELGPGLSAKDLILAIIAKLGVGGGTGHVIEYLGPAVRALSMEGRMTLCNMSIEAGA
RAGLVAPDDTTFEWLAGRPRAPKGAAWDEAVARWRALPSDDGATYDRELRLDAAALEPMITFGTNPGQGIAVTGLVPDPV
AERDASARATLEAALRYMGLVPGKPIAGQKVDVVFIGSCTNGRIEDMREAARVLKGRKVRTRTLVVPGSHRVKKDAEAEG
IDRIVREAGAEWREPGCSMCIAMNGDNLQAGQYCVSTSNRNFEGRQGPGGRTLLASPLTAAAAAVTGAVADPRRVLEGR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=397, Percent_Identity=28.463476070529, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI8659555, Length=374, Percent_Identity=24.8663101604278, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI41352693, Length=372, Percent_Identity=24.1935483870968, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=58.3333333333333, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=27.1929824561404, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.5927977839335, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367097, Length=135, Percent_Identity=36.2962962962963, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=375, Percent_Identity=29.3333333333333, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=367, Percent_Identity=29.4277929155313, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=303, Percent_Identity=29.7029702970297, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=459, Percent_Identity=25.7080610021786, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=54.8728813559322, Blast_Score=508, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6322261, Length=387, Percent_Identity=27.906976744186, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=368, Percent_Identity=29.8913043478261, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=460, Percent_Identity=26.7391304347826, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281365315, Length=376, Percent_Identity=27.9255319148936, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=376, Percent_Identity=27.9255319148936, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076999, Length=376, Percent_Identity=27.9255319148936, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=376, Percent_Identity=27.6595744680851, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137564, Length=368, Percent_Identity=27.9891304347826, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645686, Length=368, Percent_Identity=26.6304347826087, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_ANAD2 (B8J825)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492390.1
- GeneID:   7296639
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1983
- HOGENOM:   HBG330745
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50898; Mature: 50766

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRSDGSQPRTIVDKVWDAHVVRAETPDAPAILYIDLHLVHEVTSPQAFTVLRERGLKL
CCCCCCCCCCHHHHHHHHCCEEEEECCCCCCEEEEEEEEEEHHHCCCHHHHHHHHCCCCC
RRPERTLATMDHSIPTLPRGADGRWPFVDAQAAAQVSQMERNCADFGVELHGLGDDAQGV
CCCHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCEEEEECCCCCCCE
VHVFGPEMGATQPGMTVVCGDSHTATHGAFGALAFGIGTSEVGHVLASQCLLQRRPRTLA
EEEECCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
VRVDGELGPGLSAKDLILAIIAKLGVGGGTGHVIEYLGPAVRALSMEGRMTLCNMSIEAG
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCEEEEECCHHCC
ARAGLVAPDDTTFEWLAGRPRAPKGAAWDEAVARWRALPSDDGATYDRELRLDAAALEPM
CCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHEEECHHHHCCE
ITFGTNPGQGIAVTGLVPDPVAERDASARATLEAALRYMGLVPGKPIAGQKVDVVFIGSC
EEECCCCCCCEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
TNGRIEDMREAARVLKGRKVRTRTLVVPGSHRVKKDAEAEGIDRIVREAGAEWREPGCSM
CCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHCCCCCCE
CIAMNGDNLQAGQYCVSTSNRNFEGRQGPGGRTLLASPLTAAAAAVTGAVADPRRVLEGR
EEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure 
SKRSDGSQPRTIVDKVWDAHVVRAETPDAPAILYIDLHLVHEVTSPQAFTVLRERGLKL
CCCCCCCCCHHHHHHHHCCEEEEECCCCCCEEEEEEEEEEHHHCCCHHHHHHHHCCCCC
RRPERTLATMDHSIPTLPRGADGRWPFVDAQAAAQVSQMERNCADFGVELHGLGDDAQGV
CCCHHHHHHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCEEEEECCCCCCCE
VHVFGPEMGATQPGMTVVCGDSHTATHGAFGALAFGIGTSEVGHVLASQCLLQRRPRTLA
EEEECCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
VRVDGELGPGLSAKDLILAIIAKLGVGGGTGHVIEYLGPAVRALSMEGRMTLCNMSIEAG
EEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHCCCCCEEEEECCHHCC
ARAGLVAPDDTTFEWLAGRPRAPKGAAWDEAVARWRALPSDDGATYDRELRLDAAALEPM
CCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHEEECHHHHCCE
ITFGTNPGQGIAVTGLVPDPVAERDASARATLEAALRYMGLVPGKPIAGQKVDVVFIGSC
EEECCCCCCCEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
TNGRIEDMREAARVLKGRKVRTRTLVVPGSHRVKKDAEAEGIDRIVREAGAEWREPGCSM
CCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHCCCCCCE
CIAMNGDNLQAGQYCVSTSNRNFEGRQGPGGRTLLASPLTAAAAAVTGAVADPRRVLEGR
EEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA