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The map label for this gene is leuA [H]

Identifier: 220917085

GI number: 220917085

Start: 2238093

End: 2239664

Strand: Direct

Name: leuA [H]

Synonym: A2cp1_1982

Alternate gene names: 220917085

Gene position: 2238093-2239664 (Clockwise)

Preceding gene: 220917084

Following gene: 220917086

Centisome position: 44.5

GC content: 70.36

Gene sequence:

>1572_bases
GTGAGCGACGCAGGCCAAAGGGTCACCATCTTCGACACCACCCTCCGCGACGGGGAGCAGTCGCCGGGGGCGTCGATGAA
CCTGGGGCAGAAGCTCCAGGTGGCGCGGGCCCTTCGGGATCTGGGCGTGGACGTGATCGAGGCCGGCTTCGCGGCCGCCT
CCCCCGGCGATCTGGAGGCGATCCAGGCGGTCGGCCGGCAGGTCGAGGGGCCGATCGTCGCGAGCCTGTCGCGCTGCAAC
AAGGGCGACATCGAGGCGTCCTGGAAGGCGCTCGCCGACGCGCCGCGGCGCCGCTGCCACGTCTTCCTCGCCACCTCGCC
CATCCACCGCGACTTCAAGCTGAAGCTCGCGAAGGACGAGATCATCAAGCGCTCCGTGGACGGCGTGAAGCTCGCCCGCG
AGAAGTTCGACGACGTCGAGTTCTCCGCCGAGGACTCCGCCCGCACCGAGCTCGAGTTCCTGGCCGAGGTGGTCGAGCGG
GTCATCGAGGCGGGCGTCGGCACCGTGAACATCCCGGACACGGTCGGCTACGCGCTCCCCTCGACCTACGCCGAGACCAT
CCGCTACCTGCGCAAGCACGTCCGCGGCATCGAGAAGGCGGTGATCTCGGTGCACTGCCACAACGACCTGGGCATGGCGG
TCGCGAACAGCCTGGCCGGCGTGCTCGAGGGCGCGCGCCAGGTCGAGTGCACCATCAACGGCATCGGCGAGCGCGCCGGG
AACGCCTCCCTCGAGGAGATCGTCATGGCGTTCAAGACGCGCCACGACGTCCTCGGGGTGACCACCGGCGTCCAGACCGA
GCGGCTCTTCCCCACCAGCCGCCTGGTGTCGCAGGTGACCGGGCTGCACGTGCAGCGGAACAAGGCCATCGTGGGCCAGA
ACGCGTTCGCGCACGAGGCCGGCATCCACCAGCACGGGATGCTCACCAACCGCGAGACCTACGAGATCATGCGGCCCGAG
GAGGTCGGCTTCGCCCGCAGCCAGCTCGTGCTCGGCAAGCACTCCGGGCGCCACGCGCTGAAGGACCGGCTGCTCGCGCT
CGGCTACCAGCTCGACGAGGCGCAGATCGACAAGGTCTTCGCCGACTTCAAGGTGCTGGCCGACAAGAAGAAGGACATCT
ACGACGCCGACATCGAGGCGCTCGTGGTCCACGGGCAGATCCTGGGGAAGGGGAGCGTGGCCTGGGAGCTGGAGGCGCTC
TCCACCACCTCCGGCACCGGCACGCTGCCCTGCGCCTCCGTGGCGCTCCGCTCCGCCGCCGGCGAGCGGATCCAGGAGGC
CGCGGCCGGCGACGGCCCGGTGGACGCGGTGTACCGCGCCATCGAGAAGGTCACCGGCATCAGCCTCAAGCTCCGCGACT
ACCAGATCGCGAGCGTGTCCCGCGGAGAGGACGCGCAGGGCGAGGTCTCCATCGAGGTCGAGCACCCGACCGGCGTGTAC
CGCGGCCGCGCGCTCTCCACCGACATCATCGAGGGCAGCGCGCGGGCGTTCCTCGACGTGGTCAACCGGATCGCCCTGAA
GATGCCACCGCCCACCGAGAGCGAGGCCCGCGCGGAGATGGGGACGCCCTGA

Upstream 100 bases:

>100_bases
GATGGGTACACATCGCCGGACGTTTCTGGTACCCTTCGCGGTTCCCGTTTCAGCCGAGCCGGTCAGACCGAGTCAAGGTC
CCGGAAGGAGAGACGAAACC

Downstream 100 bases:

>100_bases
TGTCGAAGCGCAGCGACGGCAGCCAGCCGAGGACCATCGTCGACAAGGTCTGGGACGCGCACGTGGTCCGCGCGGAGACC
CCGGACGCGCCCGCGATCCT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSDAGQRVTIFDTTLRDGEQSPGASMNLGQKLQVARALRDLGVDVIEAGFAAASPGDLEAIQAVGRQVEGPIVASLSRCN
KGDIEASWKALADAPRRRCHVFLATSPIHRDFKLKLAKDEIIKRSVDGVKLAREKFDDVEFSAEDSARTELEFLAEVVER
VIEAGVGTVNIPDTVGYALPSTYAETIRYLRKHVRGIEKAVISVHCHNDLGMAVANSLAGVLEGARQVECTINGIGERAG
NASLEEIVMAFKTRHDVLGVTTGVQTERLFPTSRLVSQVTGLHVQRNKAIVGQNAFAHEAGIHQHGMLTNRETYEIMRPE
EVGFARSQLVLGKHSGRHALKDRLLALGYQLDEAQIDKVFADFKVLADKKKDIYDADIEALVVHGQILGKGSVAWELEAL
STTSGTGTLPCASVALRSAAGERIQEAAAGDGPVDAVYRAIEKVTGISLKLRDYQIASVSRGEDAQGEVSIEVEHPTGVY
RGRALSTDIIEGSARAFLDVVNRIALKMPPPTESEARAEMGTP

Sequences:

>Translated_523_residues
MSDAGQRVTIFDTTLRDGEQSPGASMNLGQKLQVARALRDLGVDVIEAGFAAASPGDLEAIQAVGRQVEGPIVASLSRCN
KGDIEASWKALADAPRRRCHVFLATSPIHRDFKLKLAKDEIIKRSVDGVKLAREKFDDVEFSAEDSARTELEFLAEVVER
VIEAGVGTVNIPDTVGYALPSTYAETIRYLRKHVRGIEKAVISVHCHNDLGMAVANSLAGVLEGARQVECTINGIGERAG
NASLEEIVMAFKTRHDVLGVTTGVQTERLFPTSRLVSQVTGLHVQRNKAIVGQNAFAHEAGIHQHGMLTNRETYEIMRPE
EVGFARSQLVLGKHSGRHALKDRLLALGYQLDEAQIDKVFADFKVLADKKKDIYDADIEALVVHGQILGKGSVAWELEAL
STTSGTGTLPCASVALRSAAGERIQEAAAGDGPVDAVYRAIEKVTGISLKLRDYQIASVSRGEDAQGEVSIEVEHPTGVY
RGRALSTDIIEGSARAFLDVVNRIALKMPPPTESEARAEMGTP
>Mature_522_residues
SDAGQRVTIFDTTLRDGEQSPGASMNLGQKLQVARALRDLGVDVIEAGFAAASPGDLEAIQAVGRQVEGPIVASLSRCNK
GDIEASWKALADAPRRRCHVFLATSPIHRDFKLKLAKDEIIKRSVDGVKLAREKFDDVEFSAEDSARTELEFLAEVVERV
IEAGVGTVNIPDTVGYALPSTYAETIRYLRKHVRGIEKAVISVHCHNDLGMAVANSLAGVLEGARQVECTINGIGERAGN
ASLEEIVMAFKTRHDVLGVTTGVQTERLFPTSRLVSQVTGLHVQRNKAIVGQNAFAHEAGIHQHGMLTNRETYEIMRPEE
VGFARSQLVLGKHSGRHALKDRLLALGYQLDEAQIDKVFADFKVLADKKKDIYDADIEALVVHGQILGKGSVAWELEALS
TTSGTGTLPCASVALRSAAGERIQEAAAGDGPVDAVYRAIEKVTGISLKLRDYQIASVSRGEDAQGEVSIEVEHPTGVYR
GRALSTDIIEGSARAFLDVVNRIALKMPPPTESEARAEMGTP

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=500, Percent_Identity=48.2, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320019, Length=362, Percent_Identity=32.3204419889503, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=32.2404371584699, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6324682, Length=558, Percent_Identity=24.910394265233, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=556, Percent_Identity=25.1798561151079, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56428; Mature: 56296

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAGQRVTIFDTTLRDGEQSPGASMNLGQKLQVARALRDLGVDVIEAGFAAASPGDLEA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHH
IQAVGRQVEGPIVASLSRCNKGDIEASWKALADAPRRRCHVFLATSPIHRDFKLKLAKDE
HHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHEEEEEEECCCCCCEEEEEHHHH
IIKRSVDGVKLAREKFDDVEFSAEDSARTELEFLAEVVERVIEAGVGTVNIPDTVGYALP
HHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHCCC
STYAETIRYLRKHVRGIEKAVISVHCHNDLGMAVANSLAGVLEGARQVECTINGIGERAG
HHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
NASLEEIVMAFKTRHDVLGVTTGVQTERLFPTSRLVSQVTGLHVQRNKAIVGQNAFAHEA
CCCHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHCCEEECCCEEECCCCHHHHC
GIHQHGMLTNRETYEIMRPEEVGFARSQLVLGKHSGRHALKDRLLALGYQLDEAQIDKVF
CCCCCCCCCCCCHHEEECCHHCCCHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
ADFKVLADKKKDIYDADIEALVVHGQILGKGSVAWELEALSTTSGTGTLPCASVALRSAA
HHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCEEEEEEEEECCCCCCCCHHHHHHHHHHH
GERIQEAAAGDGPVDAVYRAIEKVTGISLKLRDYQIASVSRGEDAQGEVSIEVEHPTGVY
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCEEEEEEECCCCCC
RGRALSTDIIEGSARAFLDVVNRIALKMPPPTESEARAEMGTP
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SDAGQRVTIFDTTLRDGEQSPGASMNLGQKLQVARALRDLGVDVIEAGFAAASPGDLEA
CCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHH
IQAVGRQVEGPIVASLSRCNKGDIEASWKALADAPRRRCHVFLATSPIHRDFKLKLAKDE
HHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHCCHHHEEEEEEECCCCCCEEEEEHHHH
IIKRSVDGVKLAREKFDDVEFSAEDSARTELEFLAEVVERVIEAGVGTVNIPDTVGYALP
HHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHCCC
STYAETIRYLRKHVRGIEKAVISVHCHNDLGMAVANSLAGVLEGARQVECTINGIGERAG
HHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
NASLEEIVMAFKTRHDVLGVTTGVQTERLFPTSRLVSQVTGLHVQRNKAIVGQNAFAHEA
CCCHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHCCEEECCCEEECCCCHHHHC
GIHQHGMLTNRETYEIMRPEEVGFARSQLVLGKHSGRHALKDRLLALGYQLDEAQIDKVF
CCCCCCCCCCCCHHEEECCHHCCCHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
ADFKVLADKKKDIYDADIEALVVHGQILGKGSVAWELEALSTTSGTGTLPCASVALRSAA
HHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCEEEEEEEEECCCCCCCCHHHHHHHHHHH
GERIQEAAAGDGPVDAVYRAIEKVTGISLKLRDYQIASVSRGEDAQGEVSIEVEHPTGVY
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCEEEEEEECCCCCC
RGRALSTDIIEGSARAFLDVVNRIALKMPPPTESEARAEMGTP
CCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]