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The map label for this gene is leuD

Identifier: 220917087

GI number: 220917087

Start: 2241106

End: 2241681

Strand: Direct

Name: leuD

Synonym: A2cp1_1984

Alternate gene names: 220917087

Gene position: 2241106-2241681 (Clockwise)

Preceding gene: 220917086

Following gene: 220917088

Centisome position: 44.56

GC content: 71.7

Gene sequence:

>576_bases
ATGGAACCGATCCGCGTCATCGAGAGCCGCACCGTCGTCCTCCCCCGCGAGAACGTCGACACCGACCAGATCATCCCGGC
CCGCTTCCTCAAGGTCACCGACAAGAAGGGGCTCGGCAAGGCGCTGTTCTCCGACTGGCGCTACGCCGCCGACGGCGCGC
CCAGGCCCGACTTCGTCATGAACCGGCCGGAGGCCCAGGGCTGCTCGATCCTGGTGGCCGGCGACAACTTCGGCTGCGGC
TCGTCGCGCGAGCACGCGCCCTGGGCGCTGGTGGACGCCGGCGTCCGCGCGGTGATCTCCACCCGCATCGCGGACATCTT
CCGCAACAACGCGCTCAAGAACGGCCTCGTCCCGGTGGTGCTCGACGCGGCCAGCCACGCGAAGCTGCTCGCCGCCCCCG
GCGCCAGCGTCCGCGTGGACGTCGAGGCGCAGACCGTCACGCTGCCCGACGGGTCCACCGCGCAGTTCCCGCTGGACGGG
TTCGCGCGCTACTGCCTGCTGAACGGCGTGGACGAGCTCGGCTTCCTGCTCGCGCAGGAGGCGGACATCGCCGCGTTCGA
AGGGGGCCGCCGGTGA

Upstream 100 bases:

>100_bases
GCCCCGGCGGGCGGACGCTCCTCGCCAGCCCGCTCACCGCCGCGGCCGCCGCGGTGACGGGCGCCGTCGCCGATCCCCGC
CGCGTGCTGGAGGGCCGGTG

Downstream 100 bases:

>100_bases
GCGAGGGCGCCAAGCGCCTCGCCGTTCCCGGCGGCGCCGCCGCGTCCGCGCCGTCGCGCGGCTCCGCGGGCCGCGGCATC
CGCGCCGTGGCCGTATGACG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEPIRVIESRTVVLPRENVDTDQIIPARFLKVTDKKGLGKALFSDWRYAADGAPRPDFVMNRPEAQGCSILVAGDNFGCG
SSREHAPWALVDAGVRAVISTRIADIFRNNALKNGLVPVVLDAASHAKLLAAPGASVRVDVEAQTVTLPDGSTAQFPLDG
FARYCLLNGVDELGFLLAQEADIAAFEGGRR

Sequences:

>Translated_191_residues
MEPIRVIESRTVVLPRENVDTDQIIPARFLKVTDKKGLGKALFSDWRYAADGAPRPDFVMNRPEAQGCSILVAGDNFGCG
SSREHAPWALVDAGVRAVISTRIADIFRNNALKNGLVPVVLDAASHAKLLAAPGASVRVDVEAQTVTLPDGSTAQFPLDG
FARYCLLNGVDELGFLLAQEADIAAFEGGRR
>Mature_191_residues
MEPIRVIESRTVVLPRENVDTDQIIPARFLKVTDKKGLGKALFSDWRYAADGAPRPDFVMNRPEAQGCSILVAGDNFGCG
SSREHAPWALVDAGVRAVISTRIADIFRNNALKNGLVPVVLDAASHAKLLAAPGASVRVDVEAQTVTLPDGSTAQFPLDG
FARYCLLNGVDELGFLLAQEADIAAFEGGRR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=45.360824742268, Blast_Score=169, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6321429, Length=200, Percent_Identity=41.5, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_ANAD2 (B8J826)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492391.1
- ProteinModelPortal:   B8J826
- SMR:   B8J826
- GeneID:   7296640
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1984
- HOGENOM:   HBG304838
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 20488; Mature: 20488

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIRVIESRTVVLPRENVDTDQIIPARFLKVTDKKGLGKALFSDWRYAADGAPRPDFVM
CCCCEEECCCEEEEECCCCCCCCCCHHHHEEECCCCCCCHHHHHCCCCCCCCCCCCCCEE
NRPEAQGCSILVAGDNFGCGSSREHAPWALVDAGVRAVISTRIADIFRNNALKNGLVPVV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDAASHAKLLAAPGASVRVDVEAQTVTLPDGSTAQFPLDGFARYCLLNGVDELGFLLAQE
EECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCC
ADIAAFEGGRR
CCEEECCCCCC
>Mature Secondary Structure
MEPIRVIESRTVVLPRENVDTDQIIPARFLKVTDKKGLGKALFSDWRYAADGAPRPDFVM
CCCCEEECCCEEEEECCCCCCCCCCHHHHEEECCCCCCCHHHHHCCCCCCCCCCCCCCEE
NRPEAQGCSILVAGDNFGCGSSREHAPWALVDAGVRAVISTRIADIFRNNALKNGLVPVV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDAASHAKLLAAPGASVRVDVEAQTVTLPDGSTAQFPLDGFARYCLLNGVDELGFLLAQE
EECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCC
ADIAAFEGGRR
CCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA