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The map label for this gene is glgX [H]

Identifier: 220916291

GI number: 220916291

Start: 1325519

End: 1327657

Strand: Reverse

Name: glgX [H]

Synonym: A2cp1_1184

Alternate gene names: 220916291

Gene position: 1327657-1325519 (Counterclockwise)

Preceding gene: 220916292

Following gene: 220916290

Centisome position: 26.4

GC content: 72.88

Gene sequence:

>2139_bases
ATGGACGGCGATCGCATCTGGCCTGGCCGACCCTACCCGCCGGGCGCGGTGTTCGACGGCGAGGGCACCAACTTCGCGGT
GTACGCGCGCCACGCGGACGCGGTGGAGCTGTGCCTGTTCGATCCCGCCGACCCCGCGGTCGAGCGGCGGCGACTCCGGC
TCGGCGCCCGCACCGGCCACGTCTGGCACGCCTACCTGCCCGGCGTCGGGCCGGGGACGCCCTACGGCTACCGGGCGCAC
GGTCCGTACGAGCCGGAGGCGGGGCACCGCTACAACCCGGCCAAGCTGCTGGTGGACCCGTACGCGCGCGAGCTGAGCGG
CCAGGCCGACCTGCGCGGCGCGGTGTTCGGCTACCGCCGCGGCGCGCCGGACGAGGACCTCACCCCCGACCCGGAGGACT
CCGCGCCGCGGGTGCCCCGCGCGCTGGTGGTCGGCAACCACTACGACTGGGAGGGCGACCGGCCTCCGCGCACGCCGCTG
CACCGCTCGGTCCTCTACGAGCTGCACGTGCGCGGCTTCACCATGCGTCATCCGGAGGTCCCGCCGGAGCTGCGCGGCAC
GTACGCCGGGCTGGCCTCGCCGCCCGCGCTCGAGCACCTGGCGCGCCTGGGGGTCACCGCCGTCGAGCTGCTGCCGGTGC
ACGCGTTCGTGGACGACGCGTTCCTGCTCCAGCGCGGGCTGCGCAACTACTGGGGCTACTCCACGCTCGCCTACCTCGCC
CCCGAGCCGCGCTACGCCGCGCGCCGCGGCCCCGGCGAGCAGGTGAACGAGTTCCGCGGGATGGTGAAGGCGCTGCACCG
CGCCGGCGTCGAGGTCATCCTCGACGTGGTCTTCAACCACACCTGCGAGGGCAACCACCTCGGCCCGACGCTCTCGCTGA
AGGGGCTCGACAACCGGACGTACTACCGGCTCGCGGGCGAGTCGCCGCGCTACTACGCCGACTACACCGGCACCGGCAAC
TCGCTCGACCTCACCCAGCCCCAGACGCTGGCGCTGGTGATGGACTCCCTGCGCTGCTGGGTCAACGAGATGCACGTGGA
CGGCTTCCGCCTCGACCTCGCCGTCACGCTGGCGCGCGACCCGGAGACGTTCGACGAGGCCTCGCGCTTCCTGGCGGCGG
TGCACCAGGACCCGGTGCTGCGGCAGGTGAAGCTCATCGCCGAGCCGTGGGACGTCGGGCCGGACGGCTACAAGGTCGGC
GCGTTCCCGGTGATCTGGTCGGAGTGGAACGGGAAGTACCGCGACGTGGTGCGGCGGTTCTGGAAGGGCGACGTGGACCA
GCAGGCGGAGATGGGCTACCGGCTCACCGGCTCGGCCGATCTGTACGAGCCCGCCGGGCGGAAGATCTACGCCAGCGTGA
ACTTCGTCACGGCGCACGACGGGTTCACGTTGCGCGATCTCGTCTCGTACGACCGCAAGCACAACGAGGCGAACGGCGAG
GAGAACCGCGACGGGACCGACGAGAACTTCTCCTGGAACTGCGGCGTGGAGGGAGAGACCGAAGAGCCGGCGGTGCTGGC
GCTGCGCGAGCGGCAGCAGCGGAACCTCATGGCGACGCTGCTCGTGTCGCAGGGCGTCCCGATGATCGCCGCGGGCGACG
AGATGGGGAAGACGCAGTGCGGGAACAACAACGCGTACTGCCACGACGACGAGCTGTCCTGGCTGGACTGGGACCTCGAC
GAGCGGCGCCGCGCGCTGCTCGCGTTCACCCGGCGCATGATCCGGCTGCGCCTCTCGCAGCCGGTGCTGCAGCGGCGCAG
CTTCTTCCGCGGCGCGCAGCTCTGGGACTCGTCGGTGAAGGACCTGGCCTGGTTCCGCCCCGACGGCGCCGAGATGACGC
AGGCGGACTGGGACGAGCCGTTCGCGCGGTCCGTCGCGTTCCTGCTGGGCGGCGACGCCATCGCCAGCCCCGACGAGCAC
GGGGAGCGCATCGTGGGGGACACGCTGCTCGTGCTGCTCAACGCGCACCACGAGCCGGTCCGCTACGTGCTCCCGGCGGT
GGAGTGGGGCCGGCGCTGGCTGGTGCTGGAGGACACCGCCGCCGAGCCGGAGCGCCGCCACGGGCCGGTGGAGGCCCGGG
GGGCGGTCGAGGTGGAGGCGCGCTCGCTCGTGATCCTGGCCCGGCCCGCCGAGGTGTGA

Upstream 100 bases:

>100_bases
CGGCGCGCGCTGACGCCCCACCCCACCCGGACCCACCGCCGCCGTGGCAGGCCCGCCGGCGGGGCCCCATCTTCCGGTGG
AGGCCGCGACGGAGGCGCCC

Downstream 100 bases:

>100_bases
CGGCGCCTCCGCCGCGCGCGGCGCGGGCGTCCCGGGCCGAGGTCGCCTATGATCGCGCCCGCATGGACCCGACCAAGGCG
AGCATCCTGGTGGTGGACGA

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MDGDRIWPGRPYPPGAVFDGEGTNFAVYARHADAVELCLFDPADPAVERRRLRLGARTGHVWHAYLPGVGPGTPYGYRAH
GPYEPEAGHRYNPAKLLVDPYARELSGQADLRGAVFGYRRGAPDEDLTPDPEDSAPRVPRALVVGNHYDWEGDRPPRTPL
HRSVLYELHVRGFTMRHPEVPPELRGTYAGLASPPALEHLARLGVTAVELLPVHAFVDDAFLLQRGLRNYWGYSTLAYLA
PEPRYAARRGPGEQVNEFRGMVKALHRAGVEVILDVVFNHTCEGNHLGPTLSLKGLDNRTYYRLAGESPRYYADYTGTGN
SLDLTQPQTLALVMDSLRCWVNEMHVDGFRLDLAVTLARDPETFDEASRFLAAVHQDPVLRQVKLIAEPWDVGPDGYKVG
AFPVIWSEWNGKYRDVVRRFWKGDVDQQAEMGYRLTGSADLYEPAGRKIYASVNFVTAHDGFTLRDLVSYDRKHNEANGE
ENRDGTDENFSWNCGVEGETEEPAVLALRERQQRNLMATLLVSQGVPMIAAGDEMGKTQCGNNNAYCHDDELSWLDWDLD
ERRRALLAFTRRMIRLRLSQPVLQRRSFFRGAQLWDSSVKDLAWFRPDGAEMTQADWDEPFARSVAFLLGGDAIASPDEH
GERIVGDTLLVLLNAHHEPVRYVLPAVEWGRRWLVLEDTAAEPERRHGPVEARGAVEVEARSLVILARPAEV

Sequences:

>Translated_712_residues
MDGDRIWPGRPYPPGAVFDGEGTNFAVYARHADAVELCLFDPADPAVERRRLRLGARTGHVWHAYLPGVGPGTPYGYRAH
GPYEPEAGHRYNPAKLLVDPYARELSGQADLRGAVFGYRRGAPDEDLTPDPEDSAPRVPRALVVGNHYDWEGDRPPRTPL
HRSVLYELHVRGFTMRHPEVPPELRGTYAGLASPPALEHLARLGVTAVELLPVHAFVDDAFLLQRGLRNYWGYSTLAYLA
PEPRYAARRGPGEQVNEFRGMVKALHRAGVEVILDVVFNHTCEGNHLGPTLSLKGLDNRTYYRLAGESPRYYADYTGTGN
SLDLTQPQTLALVMDSLRCWVNEMHVDGFRLDLAVTLARDPETFDEASRFLAAVHQDPVLRQVKLIAEPWDVGPDGYKVG
AFPVIWSEWNGKYRDVVRRFWKGDVDQQAEMGYRLTGSADLYEPAGRKIYASVNFVTAHDGFTLRDLVSYDRKHNEANGE
ENRDGTDENFSWNCGVEGETEEPAVLALRERQQRNLMATLLVSQGVPMIAAGDEMGKTQCGNNNAYCHDDELSWLDWDLD
ERRRALLAFTRRMIRLRLSQPVLQRRSFFRGAQLWDSSVKDLAWFRPDGAEMTQADWDEPFARSVAFLLGGDAIASPDEH
GERIVGDTLLVLLNAHHEPVRYVLPAVEWGRRWLVLEDTAAEPERRHGPVEARGAVEVEARSLVILARPAEV
>Mature_712_residues
MDGDRIWPGRPYPPGAVFDGEGTNFAVYARHADAVELCLFDPADPAVERRRLRLGARTGHVWHAYLPGVGPGTPYGYRAH
GPYEPEAGHRYNPAKLLVDPYARELSGQADLRGAVFGYRRGAPDEDLTPDPEDSAPRVPRALVVGNHYDWEGDRPPRTPL
HRSVLYELHVRGFTMRHPEVPPELRGTYAGLASPPALEHLARLGVTAVELLPVHAFVDDAFLLQRGLRNYWGYSTLAYLA
PEPRYAARRGPGEQVNEFRGMVKALHRAGVEVILDVVFNHTCEGNHLGPTLSLKGLDNRTYYRLAGESPRYYADYTGTGN
SLDLTQPQTLALVMDSLRCWVNEMHVDGFRLDLAVTLARDPETFDEASRFLAAVHQDPVLRQVKLIAEPWDVGPDGYKVG
AFPVIWSEWNGKYRDVVRRFWKGDVDQQAEMGYRLTGSADLYEPAGRKIYASVNFVTAHDGFTLRDLVSYDRKHNEANGE
ENRDGTDENFSWNCGVEGETEEPAVLALRERQQRNLMATLLVSQGVPMIAAGDEMGKTQCGNNNAYCHDDELSWLDWDLD
ERRRALLAFTRRMIRLRLSQPVLQRRSFFRGAQLWDSSVKDLAWFRPDGAEMTQADWDEPFARSVAFLLGGDAIASPDEH
GERIVGDTLLVLLNAHHEPVRYVLPAVEWGRRWLVLEDTAAEPERRHGPVEARGAVEVEARSLVILARPAEV

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=662, Percent_Identity=43.3534743202417, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80036; Mature: 80036

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGDRIWPGRPYPPGAVFDGEGTNFAVYARHADAVELCLFDPADPAVERRRLRLGARTGH
CCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCC
VWHAYLPGVGPGTPYGYRAHGPYEPEAGHRYNPAKLLVDPYARELSGQADLRGAVFGYRR
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEECHHHHHCCCCCCCCHHHHHCCC
GAPDEDLTPDPEDSAPRVPRALVVGNHYDWEGDRPPRTPLHRSVLYELHVRGFTMRHPEV
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCC
PPELRGTYAGLASPPALEHLARLGVTAVELLPVHAFVDDAFLLQRGLRNYWGYSTLAYLA
CHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PEPRYAARRGPGEQVNEFRGMVKALHRAGVEVILDVVFNHTCEGNHLGPTLSLKGLDNRT
CCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCE
YYRLAGESPRYYADYTGTGNSLDLTQPQTLALVMDSLRCWVNEMHVDGFRLDLAVTLARD
EEEECCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECC
PETFDEASRFLAAVHQDPVLRQVKLIAEPWDVGPDGYKVGAFPVIWSEWNGKYRDVVRRF
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHH
WKGDVDQQAEMGYRLTGSADLYEPAGRKIYASVNFVTAHDGFTLRDLVSYDRKHNEANGE
HCCCCCCHHHCCEEEECCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
ENRDGTDENFSWNCGVEGETEEPAVLALRERQQRNLMATLLVSQGVPMIAAGDEMGKTQC
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GNNNAYCHDDELSWLDWDLDERRRALLAFTRRMIRLRLSQPVLQRRSFFRGAQLWDSSVK
CCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAWFRPDGAEMTQADWDEPFARSVAFLLGGDAIASPDEHGERIVGDTLLVLLNAHHEPV
HHEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHEEEECCCCCCH
RYVLPAVEWGRRWLVLEDTAAEPERRHGPVEARGAVEVEARSLVILARPAEV
HHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECEEEEEECCCCC
>Mature Secondary Structure
MDGDRIWPGRPYPPGAVFDGEGTNFAVYARHADAVELCLFDPADPAVERRRLRLGARTGH
CCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCC
VWHAYLPGVGPGTPYGYRAHGPYEPEAGHRYNPAKLLVDPYARELSGQADLRGAVFGYRR
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEECHHHHHCCCCCCCCHHHHHCCC
GAPDEDLTPDPEDSAPRVPRALVVGNHYDWEGDRPPRTPLHRSVLYELHVRGFTMRHPEV
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCC
PPELRGTYAGLASPPALEHLARLGVTAVELLPVHAFVDDAFLLQRGLRNYWGYSTLAYLA
CHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PEPRYAARRGPGEQVNEFRGMVKALHRAGVEVILDVVFNHTCEGNHLGPTLSLKGLDNRT
CCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCE
YYRLAGESPRYYADYTGTGNSLDLTQPQTLALVMDSLRCWVNEMHVDGFRLDLAVTLARD
EEEECCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECC
PETFDEASRFLAAVHQDPVLRQVKLIAEPWDVGPDGYKVGAFPVIWSEWNGKYRDVVRRF
CCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHH
WKGDVDQQAEMGYRLTGSADLYEPAGRKIYASVNFVTAHDGFTLRDLVSYDRKHNEANGE
HCCCCCCHHHCCEEEECCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
ENRDGTDENFSWNCGVEGETEEPAVLALRERQQRNLMATLLVSQGVPMIAAGDEMGKTQC
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GNNNAYCHDDELSWLDWDLDERRRALLAFTRRMIRLRLSQPVLQRRSFFRGAQLWDSSVK
CCCCCEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAWFRPDGAEMTQADWDEPFARSVAFLLGGDAIASPDEHGERIVGDTLLVLLNAHHEPV
HHEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHEEEECCCCCCH
RYVLPAVEWGRRWLVLEDTAAEPERRHGPVEARGAVEVEARSLVILARPAEV
HHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]