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The map label for this gene is eno

Identifier: 220905478

GI number: 220905478

Start: 2672239

End: 2673537

Strand: Reverse

Name: eno

Synonym: Ddes_2216

Alternate gene names: 220905478

Gene position: 2673537-2672239 (Counterclockwise)

Preceding gene: 220905479

Following gene: 220905477

Centisome position: 93.04

GC content: 57.97

Gene sequence:

>1299_bases
ATGAGCAGCATTGCCTCGGTATTTGGCCGTGAAATTCTGGATTCGCGCGGTAATCCCACCGTCGAGGTGGAAGTGACCCT
GGAATCCGGGCTCAGAGCGCGGGCCGCTGTGCCTTCCGGGGCGTCCACCGGCAGCCGCGAAGCTCTGGAAATGCGTGATG
GCGACAAAGCACGGTACTGCGGCAAGGGCGTCACCAAGGCCGTGGACCATGTGAACAGCGAGATCGCCGACGCCCTGCTT
GGCATGGATTCGCTGCGTCAGGTGCAGATAGACAATACCCTTATCGATCTGGACGGCACGGACAACAAGTCCCGGCTCGG
GGCCAATGCCATGCTTGGCGTGTCCATGGCCTGCGCCAGGGTGGCGGCGTCCTTTCTGGGGCTGCCCCTGTACAAGTACC
TCGGCGGCATCAACGCCAAGGTGCTGCCAGCGCCCATGATGAACATCATCAATGGCGGGGCGCATGCTCCCAACAACCTG
GATATCCAGGAATTCATGATCATGCCCGTGGGGGCCATGACCTTTCGCGATTCGCTGCGTATGGGGACAGAAATCTTCCA
CATGCTGCAGGCCATTCTTAAAAAGGACGGGCACGTCACCAGCGTGGGTGATGAGGGCGGCTTTGCCCCCAACCTCAAGA
ATCACGATGAGGCCTTCGCTTACATCATCAAGGCGATCGAAGAAGCGGGCTATAATCCCGGCACCGAGGTGGCGCTGGCT
ATTGATGCCGCCTCCAGCGAATTCTACAAGGACGGTAAATACGTTCTTGCCGGAGAAGGCAAAACCTTCAACAGTGCCGA
ACTGAGCGAGTGGATGGCCGAATTCACCCGCAAGTACCCCCTTATATCCATTGAAGACGGCATGGCCGAAAGCGATTGGG
ACGGCTGGGGCATGCTGACCGCCAGCCTGGGCGATCATGTGCAGCTTGTGGGCGACGACGTTTTTGTGACCAATCCCTCC
ATTCTGGCCGAGGGCATTGCCGAGGGCGTGGCGAACTCCATCCTCATCAAGCTCAACCAGATCGGCACAGTCACAGAAAC
GCTGGATACCATTGAAATGGCTAAGGAAGCTGCCTATACCACCGTTATTTCGCACCGTTCCGGCGAAACGGAAGACAGTT
TCATTGCGGACCTGGCCGTGGGCGTCAACTCCGGGCAGATCAAAACCGGGTCCCTGTGTCGTTCCGAGCGTATGTCCAAG
TACAACCAGCTGCTGCGCATTGAAGAAGAACTGGGCGACGATGCCGAGTTTTTCGGTCCCATGCTGGCGGAATACTATTC
GCTGGGTACAGAAGAATAA

Upstream 100 bases:

>100_bases
AAATTTTTAAATGTCCATTCCCAGCGGTACAATGCGCTGATTCGCAGATGCGTCATATTGACTGATTGAAAACAAGCCCA
AGAGGGCAAAGGAGCCACAC

Downstream 100 bases:

>100_bases
AGCCGGTTCTGCCGGAAATGCCTGTTCGCGCTGTGCCGGTCCATGCCCCGTCGCAAAGCGGCGTGCTGCCGCCAGAGGCC
GCATCCGCCCGGGCCGGGAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSSIASVFGREILDSRGNPTVEVEVTLESGLRARAAVPSGASTGSREALEMRDGDKARYCGKGVTKAVDHVNSEIADALL
GMDSLRQVQIDNTLIDLDGTDNKSRLGANAMLGVSMACARVAASFLGLPLYKYLGGINAKVLPAPMMNIINGGAHAPNNL
DIQEFMIMPVGAMTFRDSLRMGTEIFHMLQAILKKDGHVTSVGDEGGFAPNLKNHDEAFAYIIKAIEEAGYNPGTEVALA
IDAASSEFYKDGKYVLAGEGKTFNSAELSEWMAEFTRKYPLISIEDGMAESDWDGWGMLTASLGDHVQLVGDDVFVTNPS
ILAEGIAEGVANSILIKLNQIGTVTETLDTIEMAKEAAYTTVISHRSGETEDSFIADLAVGVNSGQIKTGSLCRSERMSK
YNQLLRIEEELGDDAEFFGPMLAEYYSLGTEE

Sequences:

>Translated_432_residues
MSSIASVFGREILDSRGNPTVEVEVTLESGLRARAAVPSGASTGSREALEMRDGDKARYCGKGVTKAVDHVNSEIADALL
GMDSLRQVQIDNTLIDLDGTDNKSRLGANAMLGVSMACARVAASFLGLPLYKYLGGINAKVLPAPMMNIINGGAHAPNNL
DIQEFMIMPVGAMTFRDSLRMGTEIFHMLQAILKKDGHVTSVGDEGGFAPNLKNHDEAFAYIIKAIEEAGYNPGTEVALA
IDAASSEFYKDGKYVLAGEGKTFNSAELSEWMAEFTRKYPLISIEDGMAESDWDGWGMLTASLGDHVQLVGDDVFVTNPS
ILAEGIAEGVANSILIKLNQIGTVTETLDTIEMAKEAAYTTVISHRSGETEDSFIADLAVGVNSGQIKTGSLCRSERMSK
YNQLLRIEEELGDDAEFFGPMLAEYYSLGTEE
>Mature_431_residues
SSIASVFGREILDSRGNPTVEVEVTLESGLRARAAVPSGASTGSREALEMRDGDKARYCGKGVTKAVDHVNSEIADALLG
MDSLRQVQIDNTLIDLDGTDNKSRLGANAMLGVSMACARVAASFLGLPLYKYLGGINAKVLPAPMMNIINGGAHAPNNLD
IQEFMIMPVGAMTFRDSLRMGTEIFHMLQAILKKDGHVTSVGDEGGFAPNLKNHDEAFAYIIKAIEEAGYNPGTEVALAI
DAASSEFYKDGKYVLAGEGKTFNSAELSEWMAEFTRKYPLISIEDGMAESDWDGWGMLTASLGDHVQLVGDDVFVTNPSI
LAEGIAEGVANSILIKLNQIGTVTETLDTIEMAKEAAYTTVISHRSGETEDSFIADLAVGVNSGQIKTGSLCRSERMSKY
NQLLRIEEELGDDAEFFGPMLAEYYSLGTEE

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=52.2041763341067, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=49.8834498834499, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=48.9559164733179, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=48.9559164733179, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=44.9648711943794, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=26.4094955489614, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=26.4094955489614, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=26.4094955489614, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=62.0283018867924, Blast_Score=530, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=50.8158508158508, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=50.8158508158508, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=303, Percent_Identity=39.2739273927393, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=50.5773672055427, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.1154734411085, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.1154734411085, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=48.3796296296296, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=48.1481481481481, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_DESDA (B8J467)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480790.1
- ProteinModelPortal:   B8J467
- GeneID:   7285932
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2216
- HOGENOM:   HBG726599
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46412; Mature: 46281

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIASVFGREILDSRGNPTVEVEVTLESGLRARAAVPSGASTGSREALEMRDGDKARYC
CCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHCCCCCHHHH
GKGVTKAVDHVNSEIADALLGMDSLRQVQIDNTLIDLDGTDNKSRLGANAMLGVSMACAR
CCHHHHHHHHHHHHHHHHHHCHHHHHEEEECCEEEECCCCCCHHHCCCHHHHHHHHHHHH
VAASFLGLPLYKYLGGINAKVLPAPMMNIINGGAHAPNNLDIQEFMIMPVGAMTFRDSLR
HHHHHHCCHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
MGTEIFHMLQAILKKDGHVTSVGDEGGFAPNLKNHDEAFAYIIKAIEEAGYNPGTEVALA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
IDAASSEFYKDGKYVLAGEGKTFNSAELSEWMAEFTRKYPLISIEDGMAESDWDGWGMLT
EECCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEE
ASLGDHVQLVGDDVFVTNPSILAEGIAEGVANSILIKLNQIGTVTETLDTIEMAKEAAYT
ECCCCCEEEECCCEEEECHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
TVISHRSGETEDSFIADLAVGVNSGQIKTGSLCRSERMSKYNQLLRIEEELGDDAEFFGP
HHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MLAEYYSLGTEE
HHHHHHHCCCCC
>Mature Secondary Structure 
SSIASVFGREILDSRGNPTVEVEVTLESGLRARAAVPSGASTGSREALEMRDGDKARYC
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHCCCCCHHHH
GKGVTKAVDHVNSEIADALLGMDSLRQVQIDNTLIDLDGTDNKSRLGANAMLGVSMACAR
CCHHHHHHHHHHHHHHHHHHCHHHHHEEEECCEEEECCCCCCHHHCCCHHHHHHHHHHHH
VAASFLGLPLYKYLGGINAKVLPAPMMNIINGGAHAPNNLDIQEFMIMPVGAMTFRDSLR
HHHHHHCCHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
MGTEIFHMLQAILKKDGHVTSVGDEGGFAPNLKNHDEAFAYIIKAIEEAGYNPGTEVALA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
IDAASSEFYKDGKYVLAGEGKTFNSAELSEWMAEFTRKYPLISIEDGMAESDWDGWGMLT
EECCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEE
ASLGDHVQLVGDDVFVTNPSILAEGIAEGVANSILIKLNQIGTVTETLDTIEMAKEAAYT
ECCCCCEEEECCCEEEECHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
TVISHRSGETEDSFIADLAVGVNSGQIKTGSLCRSERMSKYNQLLRIEEELGDDAEFFGP
HHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MLAEYYSLGTEE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA