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The map label for this gene is folD

Identifier: 220905477

GI number: 220905477

Start: 2671035

End: 2671892

Strand: Reverse

Name: folD

Synonym: Ddes_2215

Alternate gene names: 220905477

Gene position: 2671892-2671035 (Counterclockwise)

Preceding gene: 220905478

Following gene: 220905476

Centisome position: 92.99

GC content: 62.82

Gene sequence:

>858_bases
ATGCTAATAATTGACGGCAAAAAGACCGCGCAGGACATCCGCGCGGAACTGGCTACAGAGGTGACCGCAGCCACGGCCGA
AGGACGCCGCGCCCCCGGTCTGGCGGTAATTCTGGTTGGCGAGGATCCGGCCTCGCAGGTATACGTGCGCAACAAGGAAA
AAGCCTGCACCGAGGTGGGTATTACCTCCTTTGCCTATCGCCTGCCGGCTGAAACCGGACAGCAGGAATTGCTGGACCTG
ATTGCCGAATGCAATGCGCGCCCCGATGTGGACGGCATTTTGCTGCAGCTGCCCCTGCCCCGCGGGCTTGACGCCCAGGC
CTGCCTGCTGGCTATTGATCCCGCCAAGGACGTGGACGGCTTTCATCCCGAAAACGTAGGACGCCTTTCACTGGGCCTGC
CGGGCTTTGTTTCCTGCACGCCTGCCGGTGTTATGGAGCTGCTGCGCCGCTACAACCTGCCCACCAGGGGTAAAAAGGCC
GTGGTGGTGGGCCGGTCGGACATTGTGGGCAAACCGCTTGCCCTGCTGCTGACCCGTTCCGGTGAGTTTGGCGACGCCAC
TGTTACCATCTGCCATTCGCGCACCCCCGACCTGGCTGAAGAGTGCCGCAAGGCCGATTTTCTGTTCCTTGCCGTGGGCA
GGCCCCGCCTCATTACCGGCGACATGGTGCGCCAGGGCGCAGTGGTCATTGACGTGGGCATCAACCGCAGCGACGATGGC
CTGTGCGGCGATGCGGACTATGAAAGTGTCAGTAAAAAGGCCGCGGCCATCACGCCCGTACCCGGCGGCGTGGGACCGAT
GACCATTGCCATGCTGCTTGTGAACACGGTGCAGTCGTGGCGGCAACGCACGGCCTGA

Upstream 100 bases:

>100_bases
GCCCTTGAGCTTGAAGCCGGGGCAGTCTATACTGCTAACATCGCATGACTATACGTTGTTGCCCTCCTGCGGGCCAAGAA
AAAAATACATGGAGTAAGCC

Downstream 100 bases:

>100_bases
TCCGTTTTCTGATATTTTTGCCCTGCCCCCTCCGGCGGAGGGGGCAGGAGACCTCGACCCGCCATAAGGCCTGCAAGGAG
CACGCATGGATTTTAAAACC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MLIIDGKKTAQDIRAELATEVTAATAEGRRAPGLAVILVGEDPASQVYVRNKEKACTEVGITSFAYRLPAETGQQELLDL
IAECNARPDVDGILLQLPLPRGLDAQACLLAIDPAKDVDGFHPENVGRLSLGLPGFVSCTPAGVMELLRRYNLPTRGKKA
VVVGRSDIVGKPLALLLTRSGEFGDATVTICHSRTPDLAEECRKADFLFLAVGRPRLITGDMVRQGAVVIDVGINRSDDG
LCGDADYESVSKKAAAITPVPGGVGPMTIAMLLVNTVQSWRQRTA

Sequences:

>Translated_285_residues
MLIIDGKKTAQDIRAELATEVTAATAEGRRAPGLAVILVGEDPASQVYVRNKEKACTEVGITSFAYRLPAETGQQELLDL
IAECNARPDVDGILLQLPLPRGLDAQACLLAIDPAKDVDGFHPENVGRLSLGLPGFVSCTPAGVMELLRRYNLPTRGKKA
VVVGRSDIVGKPLALLLTRSGEFGDATVTICHSRTPDLAEECRKADFLFLAVGRPRLITGDMVRQGAVVIDVGINRSDDG
LCGDADYESVSKKAAAITPVPGGVGPMTIAMLLVNTVQSWRQRTA
>Mature_285_residues
MLIIDGKKTAQDIRAELATEVTAATAEGRRAPGLAVILVGEDPASQVYVRNKEKACTEVGITSFAYRLPAETGQQELLDL
IAECNARPDVDGILLQLPLPRGLDAQACLLAIDPAKDVDGFHPENVGRLSLGLPGFVSCTPAGVMELLRRYNLPTRGKKA
VVVGRSDIVGKPLALLLTRSGEFGDATVTICHSRTPDLAEECRKADFLFLAVGRPRLITGDMVRQGAVVIDVGINRSDDG
LCGDADYESVSKKAAAITPVPGGVGPMTIAMLLVNTVQSWRQRTA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=47.4402730375427, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI94721354, Length=288, Percent_Identity=49.3055555555556, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=40.4109589041096, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI36796743, Length=214, Percent_Identity=30.8411214953271, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=52.8776978417266, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=261, Percent_Identity=41.3793103448276, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=43.9862542955326, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=39.4648829431438, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=26.9592476489028, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=51.1945392491468, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=51.1945392491468, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=45.8333333333333, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=45.8333333333333, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=45.8333333333333, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=45.8333333333333, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_DESDA (B8J466)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480789.1
- GeneID:   7285931
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2215
- HOGENOM:   HBG328751
- ProtClustDB:   PRK14184
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30298; Mature: 30298

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIDGKKTAQDIRAELATEVTAATAEGRRAPGLAVILVGEDPASQVYVRNKEKACTEVG
CEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHH
ITSFAYRLPAETGQQELLDLIAECNARPDVDGILLQLPLPRGLDAQACLLAIDPAKDVDG
HHHHHEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCC
FHPENVGRLSLGLPGFVSCTPAGVMELLRRYNLPTRGKKAVVVGRSDIVGKPLALLLTRS
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEECC
GEFGDATVTICHSRTPDLAEECRKADFLFLAVGRPRLITGDMVRQGAVVIDVGINRSDDG
CCCCCEEEEEEECCCCHHHHHHHHCCEEEEEECCCCEEEHHHHHCCCEEEEECCCCCCCC
LCGDADYESVSKKAAAITPVPGGVGPMTIAMLLVNTVQSWRQRTA
CCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIIDGKKTAQDIRAELATEVTAATAEGRRAPGLAVILVGEDPASQVYVRNKEKACTEVG
CEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHH
ITSFAYRLPAETGQQELLDLIAECNARPDVDGILLQLPLPRGLDAQACLLAIDPAKDVDG
HHHHHEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCC
FHPENVGRLSLGLPGFVSCTPAGVMELLRRYNLPTRGKKAVVVGRSDIVGKPLALLLTRS
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEECC
GEFGDATVTICHSRTPDLAEECRKADFLFLAVGRPRLITGDMVRQGAVVIDVGINRSDDG
CCCCCEEEEEEECCCCHHHHHHHHCCEEEEEECCCCEEEHHHHHCCCEEEEECCCCCCCC
LCGDADYESVSKKAAAITPVPGGVGPMTIAMLLVNTVQSWRQRTA
CCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA