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The map label for this gene is neoB [H]

Identifier: 220905474

GI number: 220905474

Start: 2667594

End: 2668781

Strand: Reverse

Name: neoB [H]

Synonym: Ddes_2212

Alternate gene names: 220905474

Gene position: 2668781-2667594 (Counterclockwise)

Preceding gene: 220905475

Following gene: 220905473

Centisome position: 92.88

GC content: 60.35

Gene sequence:

>1188_bases
ATGGATATCAAGGTCAATTTCAGCGGACGTGCCATCAGGTACACCGAAGAAGAAATAGCCGTGGTTGTCGAGGCCATGCG
CAACGCCGATCCCCTTACCCAGGGGCATCACATGCGCCGGTTTGAGAGCAGGTTCGCAGAGTACCAGGGGGTGCCGCAGG
GAACCTGCTTTACCACCATGAACGGCTGCTCCGCCCTGGAACTTTCAGCGCAGCTCTGCCTGTTCAATGAAGGCGACGAG
GTGGTCATTCCCTCCCATACCTTCACGGCCTCGGCCTATCCCTACATCAAGAAGGGGGCCAGGCTTGTCTGGGCCGATGT
GGACCTGCACAGCCGCGTGGTCACCGCCGAAAGCATTGAAAAAGTGCTTACCCCGCGTACCAGGGCCGTGGTGGTCGTGC
ACCTTTACGGCTATGTGGCCGACATGCCCGCCATTGCCGCCCTGTGCAAGGAACGCGGCCTTATCCTCATCGAGGACGCC
GCCCAGGCCATAGGGGCCGAAGTAAACGGCATCAAGGCCGGAGCCTTCGGGGATATGGGGGTCTTCTCGTTCCACTCGCA
CAAAAACCTGACCACCCTGGGCGAAGGCGGCATGCTGTATGTGCGCGACCCCAAGCTGGCGGGCATGGTTCCCACCCTGC
GCCACAACGGGCATTGCGCCTTTGATTTTGAGCGCCCCGATTACTGGAAGCCCGCCATGGGCAATGTGGACATGCCCTTT
ATCGACGGCCGCATGGTGCCGCCCAACAACTACTGCCTGGGCGAAGTGGAATGCGCCCTCGGAACCAAGCTGCTGGAACG
TATTGACGAGATCAACGGGCAGAAGCGCAGCCGCGCCCTGGCCTTCATTGATGCCCTGGCGGACTTTCCCGAACTGGAAT
TCCATCGGGTCGACAGCCCCCGCCACAACTACCATCTGCTTGCGGCCCGCATGACCACGGGCGTGGAGGCGCGGGATCGC
TTCATGCGCGCCATGTTCAATGAAAAGGGCGTCAAATGCGTGGTGCAGTATATCCCGCTGGACAGGTACGACTATTACCG
CCGCCTGGGCATGGGCGAGGCCTGTTGCCCCAATGCGGACCTGTTCTTTGACACTATGATTTCTTTCCCCTTCCAGCACT
GGCTGACCGGGGAAGAATTTGACTATATGCTCGCTTCCACCCGCGAGGTACTGGCGAGCCTGCGCTAA

Upstream 100 bases:

>100_bases
ATGCGGAACGCGCGCAGCAACGGTTGCAGGACTGGCGCGTCCGGGCCTTGCCCGCTGTGACGCGACAATTTCAAGCGCAA
ACTGCTTTATGAGGAGTCCC

Downstream 100 bases:

>100_bases
GGCGGCGTATCTGCCGTCCGGCCGCGTCAGAAATAACGGCGTGGGCCGGGAATGGTACTCAAGGGTACTGGCCTGGCCTG
CGCCGCTGTGTTAGCCGGGC

Product: DegT/DnrJ/EryC1/StrS aminotransferase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: L-glutamine:DOI aminotransferase; Bifunctional L-glutamine:ketocyclitol aminotransferase I/II; L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase; L-glutamine:amino-DOI aminotransferase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MDIKVNFSGRAIRYTEEEIAVVVEAMRNADPLTQGHHMRRFESRFAEYQGVPQGTCFTTMNGCSALELSAQLCLFNEGDE
VVIPSHTFTASAYPYIKKGARLVWADVDLHSRVVTAESIEKVLTPRTRAVVVVHLYGYVADMPAIAALCKERGLILIEDA
AQAIGAEVNGIKAGAFGDMGVFSFHSHKNLTTLGEGGMLYVRDPKLAGMVPTLRHNGHCAFDFERPDYWKPAMGNVDMPF
IDGRMVPPNNYCLGEVECALGTKLLERIDEINGQKRSRALAFIDALADFPELEFHRVDSPRHNYHLLAARMTTGVEARDR
FMRAMFNEKGVKCVVQYIPLDRYDYYRRLGMGEACCPNADLFFDTMISFPFQHWLTGEEFDYMLASTREVLASLR

Sequences:

>Translated_395_residues
MDIKVNFSGRAIRYTEEEIAVVVEAMRNADPLTQGHHMRRFESRFAEYQGVPQGTCFTTMNGCSALELSAQLCLFNEGDE
VVIPSHTFTASAYPYIKKGARLVWADVDLHSRVVTAESIEKVLTPRTRAVVVVHLYGYVADMPAIAALCKERGLILIEDA
AQAIGAEVNGIKAGAFGDMGVFSFHSHKNLTTLGEGGMLYVRDPKLAGMVPTLRHNGHCAFDFERPDYWKPAMGNVDMPF
IDGRMVPPNNYCLGEVECALGTKLLERIDEINGQKRSRALAFIDALADFPELEFHRVDSPRHNYHLLAARMTTGVEARDR
FMRAMFNEKGVKCVVQYIPLDRYDYYRRLGMGEACCPNADLFFDTMISFPFQHWLTGEEFDYMLASTREVLASLR
>Mature_395_residues
MDIKVNFSGRAIRYTEEEIAVVVEAMRNADPLTQGHHMRRFESRFAEYQGVPQGTCFTTMNGCSALELSAQLCLFNEGDE
VVIPSHTFTASAYPYIKKGARLVWADVDLHSRVVTAESIEKVLTPRTRAVVVVHLYGYVADMPAIAALCKERGLILIEDA
AQAIGAEVNGIKAGAFGDMGVFSFHSHKNLTTLGEGGMLYVRDPKLAGMVPTLRHNGHCAFDFERPDYWKPAMGNVDMPF
IDGRMVPPNNYCLGEVECALGTKLLERIDEINGQKRSRALAFIDALADFPELEFHRVDSPRHNYHLLAARMTTGVEARDR
FMRAMFNEKGVKCVVQYIPLDRYDYYRRLGMGEACCPNADLFFDTMISFPFQHWLTGEEFDYMLASTREVLASLR

Specific function: Catalyzes the PLP-dependent transamination of 2-deoxy- scyllo-inosose (DOI) to form 2-deoxy-scyllo-inosamine (DOIA) using L-glutamine as the amino donor. Also catalyzes the transamination of 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI) into 2- deoxystre

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. L-glutamine:2- deoxy-scyllo-inosose/scyllo-inosose aminotransferase subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=333, Percent_Identity=28.2282282282282, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI145693159, Length=381, Percent_Identity=27.0341207349081, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 44421; Mature: 44421

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKVNFSGRAIRYTEEEIAVVVEAMRNADPLTQGHHMRRFESRFAEYQGVPQGTCFTTM
CEEEEECCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
NGCSALELSAQLCLFNEGDEVVIPSHTFTASAYPYIKKGARLVWADVDLHSRVVTAESIE
CCCCEEEEEEEEEEECCCCCEEECCCCCCCCCCHHHHCCCEEEEEECCCCHHEECHHHHH
KVLTPRTRAVVVVHLYGYVADMPAIAALCKERGLILIEDAAQAIGAEVNGIKAGAFGDMG
HHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHCCCCCCCEEECCCCCCC
VFSFHSHKNLTTLGEGGMLYVRDPKLAGMVPTLRHNGHCAFDFERPDYWKPAMGNVDMPF
EEEEECCCCCEEECCCCEEEEECCCCCCCCCHHCCCCCEEEECCCCCCCCCCCCCCCCCE
IDGRMVPPNNYCLGEVECALGTKLLERIDEINGQKRSRALAFIDALADFPELEFHRVDSP
ECCEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCC
RHNYHLLAARMTTGVEARDRFMRAMFNEKGVKCVVQYIPLDRYDYYRRLGMGEACCPNAD
CCCEEEEEEEHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCC
LFFDTMISFPFQHWLTGEEFDYMLASTREVLASLR
HHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIKVNFSGRAIRYTEEEIAVVVEAMRNADPLTQGHHMRRFESRFAEYQGVPQGTCFTTM
CEEEEECCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEC
NGCSALELSAQLCLFNEGDEVVIPSHTFTASAYPYIKKGARLVWADVDLHSRVVTAESIE
CCCCEEEEEEEEEEECCCCCEEECCCCCCCCCCHHHHCCCEEEEEECCCCHHEECHHHHH
KVLTPRTRAVVVVHLYGYVADMPAIAALCKERGLILIEDAAQAIGAEVNGIKAGAFGDMG
HHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCEEEEECHHHHCCCCCCCEEECCCCCCC
VFSFHSHKNLTTLGEGGMLYVRDPKLAGMVPTLRHNGHCAFDFERPDYWKPAMGNVDMPF
EEEEECCCCCEEECCCCEEEEECCCCCCCCCHHCCCCCEEEECCCCCCCCCCCCCCCCCE
IDGRMVPPNNYCLGEVECALGTKLLERIDEINGQKRSRALAFIDALADFPELEFHRVDSP
ECCEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCC
RHNYHLLAARMTTGVEARDRFMRAMFNEKGVKCVVQYIPLDRYDYYRRLGMGEACCPNAD
CCCEEEEEEEHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCC
LFFDTMISFPFQHWLTGEEFDYMLASTREVLASLR
HHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12546424 [H]