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The map label for this gene is lutP [H]

Identifier: 220905432

GI number: 220905432

Start: 2615858

End: 2617495

Strand: Reverse

Name: lutP [H]

Synonym: Ddes_2170

Alternate gene names: 220905432

Gene position: 2617495-2615858 (Counterclockwise)

Preceding gene: 220905433

Following gene: 220905431

Centisome position: 91.09

GC content: 60.5

Gene sequence:

>1638_bases
ATGGCATGGACGCAAGTTTACGATCCGATGGGCGGAGCCGTATGGTCCGCACTGGTAGCGGGGATTCCGCTCATCAGTCT
TTTTTACATGCTGGCTGTACGCCGCGCCAAAGGACATTACGCCGCGGCTCTGGCCGTGGGCCTGTCTTTTGTTCTCGCCC
TCTTTGTGTGGGGCATGCCTGTGGGCACGGCCTTCAGCGCGTTGACCTACGGGGCAGCCTTTGGCCTCTTTCCGATTATC
TGGATCGTGGTCACGGCGGTCTGGGTGTACAACATGACCGTGGAATCGGGCGAATTTGAGTACATCAAGGAATCGCTCGC
CCGCCTGACAGACGACCGCCGTCTGCAGGCCATCTTTATCGCCTTTGCTTTCGGTTCGTTCATTGAAGGCACGGCCGGCT
TCGGTACGCCCGTGGCCATCACCGCGGCAATGCTGGTGGGCTTGGGCTTCAAGCCCCTGTACGCCGCCGGTATCTGCCTT
ATCGCCAACACCGCTCCCGTGGCCTTCGGCGCCATCGGCATTCCGATCATCGTGGGCGCTCAGGTTTCCGGCCTGAACGA
AATGACCGTGAGCCAGATTGTGGGTCGCCAGCTGCCCTTCCTGTCCATCATAGTGCCTCTATGGCTGTGCTGCGTCATGT
GCGGCTTCCGCCGTTCGCTGGAAGTGCTGCCCGCCATCATCGTGGCCGGTGTGGCTTTTGCCGGTTCGCAGTTCCTGTTC
TCCAACTTCCACGGCCCGACCCTGCCCGACATCATGTCGGCCCTGGTCACCATCATCGCCATGGTGCTGCTGCTGCGCGT
GTGGAAGCCTGCCCGCGTCTGGCGCTTTGAAGGCGAAAAGGAAAGCAAGATCGAAGGATCTGCGCCGCCCTTCAGTGTGG
TGTTCCGCGCATGGCTGCCCTACATCATTCTGGCTGTGATGGTCTTCCTGTGGGGCCTGCCGCAGTTCAAGAACATGCTG
AACGCCATTCCCGGCGCCATCATGAAGTTTTCCTGGCCCGGCCTGGACGGCATGGTTGAAAAGACCGCCCCCATCGTGGC
CCAGAACACCAACTACGCGGCCGTGTTTACGTTCAACTGGCTGTCTGCCGGCGGCACCGCCATCCTGCTGGCCGGTTTCA
TTGCCGTGCCCTTCATGCCCGGCTACTCTTTCAGCAAGGCTGTAAACTGCTTCTTCCGCACCATTTACCAGCTGCGCTTC
CCCATCCTGACCATCGCCATGATTCTTGGCCTGGCCTATCTCATGAACTACTCGGGCATGAGTTCCACAATGGGTATTGC
CTTTACCCTGACCGGCTCGCTGTTCCCGCTGTTCTCGCCCATTCTGGGCTGGCTCGGCGTGTTTCTGACCGGTTCGGATA
CGTCCTCCAACGCCCTGTTCTGCGGTATGCAGCGCTCCACCGCTGAAGTGGTGGGCATGGACCCGAACCTGGCCGTGGCC
GCCAACTCCTCCGGTGGTGTGACCGGTAAGATGATTTCGCCCCAGTCCATCAGCGTGGCCACAGCTGCCACCAACCTGGT
GGGCCATGAAGGCGACCTCTTCCGCTTCACTCTGGGTCACAGTATTGCAATGACCCTCTTTATCTGCGCGCTGACCTATC
TGCAGGCCGGCGTGCTGCACTGGATGCTGCCCTCCTAG

Upstream 100 bases:

>100_bases
GCTATTCTTGGGGACAGTCGGGTCCGCAGCGCGTGTGGGGTGCACAATCGCTTGCGGGGCCGTGTCTCCATACAAACCAT
AGCGCAAGGGAGCAGTTGTT

Downstream 100 bases:

>100_bases
GGTTTTGTTTCCCTGCCGCCGCCTGTCGGACAGGCGGCGGCAGGGGCTGGCTACACCCCGCAAGATGATCACCAGGGCGG
ACCTGACCGGTACGACCGCG

Product: L-lactate transport protein

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MAWTQVYDPMGGAVWSALVAGIPLISLFYMLAVRRAKGHYAAALAVGLSFVLALFVWGMPVGTAFSALTYGAAFGLFPII
WIVVTAVWVYNMTVESGEFEYIKESLARLTDDRRLQAIFIAFAFGSFIEGTAGFGTPVAITAAMLVGLGFKPLYAAGICL
IANTAPVAFGAIGIPIIVGAQVSGLNEMTVSQIVGRQLPFLSIIVPLWLCCVMCGFRRSLEVLPAIIVAGVAFAGSQFLF
SNFHGPTLPDIMSALVTIIAMVLLLRVWKPARVWRFEGEKESKIEGSAPPFSVVFRAWLPYIILAVMVFLWGLPQFKNML
NAIPGAIMKFSWPGLDGMVEKTAPIVAQNTNYAAVFTFNWLSAGGTAILLAGFIAVPFMPGYSFSKAVNCFFRTIYQLRF
PILTIAMILGLAYLMNYSGMSSTMGIAFTLTGSLFPLFSPILGWLGVFLTGSDTSSNALFCGMQRSTAEVVGMDPNLAVA
ANSSGGVTGKMISPQSISVATAATNLVGHEGDLFRFTLGHSIAMTLFICALTYLQAGVLHWMLPS

Sequences:

>Translated_545_residues
MAWTQVYDPMGGAVWSALVAGIPLISLFYMLAVRRAKGHYAAALAVGLSFVLALFVWGMPVGTAFSALTYGAAFGLFPII
WIVVTAVWVYNMTVESGEFEYIKESLARLTDDRRLQAIFIAFAFGSFIEGTAGFGTPVAITAAMLVGLGFKPLYAAGICL
IANTAPVAFGAIGIPIIVGAQVSGLNEMTVSQIVGRQLPFLSIIVPLWLCCVMCGFRRSLEVLPAIIVAGVAFAGSQFLF
SNFHGPTLPDIMSALVTIIAMVLLLRVWKPARVWRFEGEKESKIEGSAPPFSVVFRAWLPYIILAVMVFLWGLPQFKNML
NAIPGAIMKFSWPGLDGMVEKTAPIVAQNTNYAAVFTFNWLSAGGTAILLAGFIAVPFMPGYSFSKAVNCFFRTIYQLRF
PILTIAMILGLAYLMNYSGMSSTMGIAFTLTGSLFPLFSPILGWLGVFLTGSDTSSNALFCGMQRSTAEVVGMDPNLAVA
ANSSGGVTGKMISPQSISVATAATNLVGHEGDLFRFTLGHSIAMTLFICALTYLQAGVLHWMLPS
>Mature_544_residues
AWTQVYDPMGGAVWSALVAGIPLISLFYMLAVRRAKGHYAAALAVGLSFVLALFVWGMPVGTAFSALTYGAAFGLFPIIW
IVVTAVWVYNMTVESGEFEYIKESLARLTDDRRLQAIFIAFAFGSFIEGTAGFGTPVAITAAMLVGLGFKPLYAAGICLI
ANTAPVAFGAIGIPIIVGAQVSGLNEMTVSQIVGRQLPFLSIIVPLWLCCVMCGFRRSLEVLPAIIVAGVAFAGSQFLFS
NFHGPTLPDIMSALVTIIAMVLLLRVWKPARVWRFEGEKESKIEGSAPPFSVVFRAWLPYIILAVMVFLWGLPQFKNMLN
AIPGAIMKFSWPGLDGMVEKTAPIVAQNTNYAAVFTFNWLSAGGTAILLAGFIAVPFMPGYSFSKAVNCFFRTIYQLRFP
ILTIAMILGLAYLMNYSGMSSTMGIAFTLTGSLFPLFSPILGWLGVFLTGSDTSSNALFCGMQRSTAEVVGMDPNLAVAA
NSSGGVTGKMISPQSISVATAATNLVGHEGDLFRFTLGHSIAMTLFICALTYLQAGVLHWMLPS

Specific function: Is the principal permease for the uptake of L-lactate in B.subtilis [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=558, Percent_Identity=50.5376344086022, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1790031, Length=549, Percent_Identity=48.4517304189435, Blast_Score=504, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58467; Mature: 58336

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCC
>Mature Secondary Structure 
AWTQVYDPMGGAVWSALVAGIPLISLFYMLAVRRAKGHYAAALAVGLSFVLALFVWGMP
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
VGTAFSALTYGAAFGLFPIIWIVVTAVWVYNMTVESGEFEYIKESLARLTDDRRLQAIFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHHHHHHHHHHCCCCHHHHHHH
AFAFGSFIEGTAGFGTPVAITAAMLVGLGFKPLYAAGICLIANTAPVAFGAIGIPIIVGA
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEECCCCHHHHHCCCCEEECC
QVSGLNEMTVSQIVGRQLPFLSIIVPLWLCCVMCGFRRSLEVLPAIIVAGVAFAGSQFLF
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SNFHGPTLPDIMSALVTIIAMVLLLRVWKPARVWRFEGEKESKIEGSAPPFSVVFRAWLP
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
YIILAVMVFLWGLPQFKNMLNAIPGAIMKFSWPGLDGMVEKTAPIVAQNTNYAAVFTFNW
HHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCCCCCCHHHHCCCEEEECCCEEEEEEEEC
LSAGGTAILLAGFIAVPFMPGYSFSKAVNCFFRTIYQLRFPILTIAMILGLAYLMNYSGM
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSTMGIAFTLTGSLFPLFSPILGWLGVFLTGSDTSSNALFCGMQRSTAEVVGMDPNLAVA
CCCCCEEEEEHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCHHHCCCCCCEEEE
ANSSGGVTGKMISPQSISVATAATNLVGHEGDLFRFTLGHSIAMTLFICALTYLQAGVLH
ECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
WMLPS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969506 [H]