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The map label for this gene is dhaT [H]

Identifier: 220905313

GI number: 220905313

Start: 2465430

End: 2466638

Strand: Reverse

Name: dhaT [H]

Synonym: Ddes_2051

Alternate gene names: 220905313

Gene position: 2466638-2465430 (Counterclockwise)

Preceding gene: 220905314

Following gene: 220905311

Centisome position: 85.84

GC content: 60.3

Gene sequence:

>1209_bases
ATGTGGGATGACTGCTGCGATTACAATATGGTTCGCGAAGTACGGGTAAAAGGCATTGCTTACCTCGGTGTGGGAGCCAT
AGACAAGATAGACCCCATCCTGGGCAGGTTGAAAAGCGAAGGGATAACAGCAGTTCTTTGTGTTTGCGGCGGGCATGCCT
ACCGTGCTTCGGGCGCGTGGGATAAAGCCGAGGCCGCCGCCCTCAGGCACGGCATTGTGCTGGCCCTGTACAACAGGGTT
ACCCCCAGCCCCACCACAGATACTGTGGATGACGCCGCAGCCCTTGGGCGGGCCATCAATGCCGGAGCGGTGCTTGCCAT
AGGCGGGGGCAGCCCTCTGGACTGTGGCAAGAGTGCGGCCATACTGCTGGCCAATCCCGGTAAAAGCGCTGAAGATCTTT
TTTGTTTTCGCTTCACGCCCCAGAAGGCCCTTCCTCTAGTTGCCGTCAATCTCACCCACGGCACGGGCAGTGAAGTAAAC
CGTTTTGCCGTGGTCACCGTATCGGGGCGCAGCCACAGGTCCATTGTGGGGCATGACTGCATGTACCCCCGGTACGCCAT
TGACGACCCCGCCCTTATGACCGGGCTTTCGGCAGAGCAGAGCCGCTATGTGTCCATAAATGCGTTCTGCAGGGTTGTGG
AAGCTGCCACAGCCACGGCAGCCAACCCCCTGGCGGTTACGCTGGCCCGCGAAACCGTGCGCCTGGTTCATCACTGGTTG
CCGGTGGCGCTGGATGAGCCGGAAAATCTCAAGGCCCGTTACGGGCTGTGCCTGGCAGCCCTTCAGGCCGGGGTGGCTTT
TGACAACAGCCATCTGCACCTGGCGCACGCGCTGGAGCAGCCTTTGGGCCGCTTGCGAAATCTGCCTCACGGCATGCGTC
TGGCCATTCTGCTGCCTGCTGTGATTGGCGCATGTTACGCCGCCAAGCCCGATGTGCTGGCGCATGTGCTTGGGCCGGTG
GCCCCGGGCCTGCAGGGCATGCCCGAAGAAGCTGACCAAGCGGCGCGGGCTGTGGAACGCTGGATATTCAGCCTGGGCAT
AACGCAAAAACTCCTGGACTTGGGAGTCCAGGAGTTCGATGTGGAAGAGTTTTGCAACTTCGTCGAGCAGACGCCTTCGC
TGGGTCTTCTGCTCTCGGTAGCGCCGGTCAGTATTTCCCGCCAGCTTGTGGCAAGAATTTACACTGAATCGTTGCGCCCC
ATGATGTGA

Upstream 100 bases:

>100_bases
AAAATTATGTGAAAAATTGCGCAGCTCCTTGACCTAAGCTTATATTCGTTCTAGCTTGATGCAGCGCAATAAAAGCCCAG
CGTGATCGCGGGAGGTTGCC

Downstream 100 bases:

>100_bases
AATTGCCCATCCTCCTCCAGGGCCGGACTGATCTGCCGCAGTCCGGTCCATACCCAGCCCCTCCGTTTGCCGACGGCGGG
GCTTTTTCTTATCACGCCCC

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MWDDCCDYNMVREVRVKGIAYLGVGAIDKIDPILGRLKSEGITAVLCVCGGHAYRASGAWDKAEAAALRHGIVLALYNRV
TPSPTTDTVDDAAALGRAINAGAVLAIGGGSPLDCGKSAAILLANPGKSAEDLFCFRFTPQKALPLVAVNLTHGTGSEVN
RFAVVTVSGRSHRSIVGHDCMYPRYAIDDPALMTGLSAEQSRYVSINAFCRVVEAATATAANPLAVTLARETVRLVHHWL
PVALDEPENLKARYGLCLAALQAGVAFDNSHLHLAHALEQPLGRLRNLPHGMRLAILLPAVIGACYAAKPDVLAHVLGPV
APGLQGMPEEADQAARAVERWIFSLGITQKLLDLGVQEFDVEEFCNFVEQTPSLGLLLSVAPVSISRQLVARIYTESLRP
MM

Sequences:

>Translated_402_residues
MWDDCCDYNMVREVRVKGIAYLGVGAIDKIDPILGRLKSEGITAVLCVCGGHAYRASGAWDKAEAAALRHGIVLALYNRV
TPSPTTDTVDDAAALGRAINAGAVLAIGGGSPLDCGKSAAILLANPGKSAEDLFCFRFTPQKALPLVAVNLTHGTGSEVN
RFAVVTVSGRSHRSIVGHDCMYPRYAIDDPALMTGLSAEQSRYVSINAFCRVVEAATATAANPLAVTLARETVRLVHHWL
PVALDEPENLKARYGLCLAALQAGVAFDNSHLHLAHALEQPLGRLRNLPHGMRLAILLPAVIGACYAAKPDVLAHVLGPV
APGLQGMPEEADQAARAVERWIFSLGITQKLLDLGVQEFDVEEFCNFVEQTPSLGLLLSVAPVSISRQLVARIYTESLRP
MM
>Mature_402_residues
MWDDCCDYNMVREVRVKGIAYLGVGAIDKIDPILGRLKSEGITAVLCVCGGHAYRASGAWDKAEAAALRHGIVLALYNRV
TPSPTTDTVDDAAALGRAINAGAVLAIGGGSPLDCGKSAAILLANPGKSAEDLFCFRFTPQKALPLVAVNLTHGTGSEVN
RFAVVTVSGRSHRSIVGHDCMYPRYAIDDPALMTGLSAEQSRYVSINAFCRVVEAATATAANPLAVTLARETVRLVHHWL
PVALDEPENLKARYGLCLAALQAGVAFDNSHLHLAHALEQPLGRLRNLPHGMRLAILLPAVIGACYAAKPDVLAHVLGPV
APGLQGMPEEADQAARAVERWIFSLGITQKLLDLGVQEFDVEEFCNFVEQTPSLGLLLSVAPVSISRQLVARIYTESLRP
MM

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=373, Percent_Identity=22.5201072386059, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI48994951, Length=340, Percent_Identity=30.2941176470588, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87082107, Length=287, Percent_Identity=31.0104529616725, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787493, Length=327, Percent_Identity=27.217125382263, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789163, Length=307, Percent_Identity=28.6644951140065, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789386, Length=341, Percent_Identity=26.3929618768328, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=381, Percent_Identity=23.3595800524934, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=292, Percent_Identity=31.1643835616438, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24657991, Length=374, Percent_Identity=25.4010695187166, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDDCCDYNMVREVRVKGIAYLGVGAIDKIDPILGRLKSEGITAVLCVCGGHAYRASGAW
CCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCC
DKAEAAALRHGIVLALYNRVTPSPTTDTVDDAAALGRAINAGAVLAIGGGSPLDCGKSAA
CHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCE
ILLANPGKSAEDLFCFRFTPQKALPLVAVNLTHGTGSEVNRFAVVTVSGRSHRSIVGHDC
EEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCC
MYPRYAIDDPALMTGLSAEQSRYVSINAFCRVVEAATATAANPLAVTLARETVRLVHHWL
CCCCCCCCCCHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHC
PVALDEPENLKARYGLCLAALQAGVAFDNSHLHLAHALEQPLGRLRNLPHGMRLAILLPA
CCCCCCCCCHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VIGACYAAKPDVLAHVLGPVAPGLQGMPEEADQAARAVERWIFSLGITQKLLDLGVQEFD
HHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VEEFCNFVEQTPSLGLLLSVAPVSISRQLVARIYTESLRPMM
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MWDDCCDYNMVREVRVKGIAYLGVGAIDKIDPILGRLKSEGITAVLCVCGGHAYRASGAW
CCCCCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCC
DKAEAAALRHGIVLALYNRVTPSPTTDTVDDAAALGRAINAGAVLAIGGGSPLDCGKSAA
CHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCE
ILLANPGKSAEDLFCFRFTPQKALPLVAVNLTHGTGSEVNRFAVVTVSGRSHRSIVGHDC
EEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCC
MYPRYAIDDPALMTGLSAEQSRYVSINAFCRVVEAATATAANPLAVTLARETVRLVHHWL
CCCCCCCCCCHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHC
PVALDEPENLKARYGLCLAALQAGVAFDNSHLHLAHALEQPLGRLRNLPHGMRLAILLPA
CCCCCCCCCHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VIGACYAAKPDVLAHVLGPVAPGLQGMPEEADQAARAVERWIFSLGITQKLLDLGVQEFD
HHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VEEFCNFVEQTPSLGLLLSVAPVSISRQLVARIYTESLRPMM
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]