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The map label for this gene is trpA

Identifier: 220904853

GI number: 220904853

Start: 1908136

End: 1908903

Strand: Reverse

Name: trpA

Synonym: Ddes_1587

Alternate gene names: 220904853

Gene position: 1908903-1908136 (Counterclockwise)

Preceding gene: 220904854

Following gene: 220904852

Centisome position: 66.43

GC content: 60.42

Gene sequence:

>768_bases
ATGAACCTGCTTGAAGAAAAGATACGTGCGGCCAATGCCGCCGGACGCCCGGCCCTTATCCCTTTTTTGACCGCGGGCTT
TCCCGATCGCGCAACATTTTGGCCCACATTGATGGAGCTGGATGAAAACGGAGCGGATATTATTGAAATAGGCGTGCCGT
TTTCCGATCCCGTTGCCGACGGCCCGGTGGTGGAAGAGGCTTCGCGGCGGGCGCTCAGCGACGGGGTAAACCTGCGCGGC
ATTCTTGCGGAGCTTGCCCAGCGCAAGGGCCTTGTCAAGGCCGGGCTTGTGTTGATGGGATATCTGAATCCCTTTTTGCA
GTATGGCTACGAAAAACTGGCGCAGGATGCAGCCCGGGGCGGTGTGCATGGCTTCATCGTGCCCGACCTGCCCCATGAAG
AGTCCGGGCCGCTGCATCGTGCCCTGAAGAAAGAGGGCATCGCCCTTATCCCGCTGGTGGGACCCAATACCAGTGCGGAG
CGCATGGCCCTGTATGCCGAAGAAGGCGAGGGCTATGTGTATGTGGTTTCGGTTATGGGCATTACCGGAGAACGGGGAGA
TGTAGCTCCGCAGGTGGCCGATACCATGCGGCGGGCGCGTTCTGTCTTCAGCCTTCCCTTGGCTTTAGGCTTCGGTCTGC
GTGAGCCGGGGCAGCTCAGGGAACTTCCGCCGGACGCCAGGCCGGATGCCGCGGTTTTCGGCAGTGCCCTGCTCAACCAC
CTTGAAGAAGGCCACAGCGCCGAAGAGTTTATGGCCCGCTGGAAATAG

Upstream 100 bases:

>100_bases
GCCAGCAGGTGGTGGTCAACCTTTCGGGCCGCGGTGACAAGGATCTTGGCATTATCAACAAGGCCCTGGGTTTTGCCCTG
GGGCAGGACGAGGTGTAGTC

Downstream 100 bases:

>100_bases
AGCAGTCTTACACTCTGTTGCTACGGCGACGCGCAAAGGGCTCTGCCGCGAAGGGGCGGTACAGTTTGTTTGTGCTGTCA
AGCGCCGGAGCGGACGTGCC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNLLEEKIRAANAAGRPALIPFLTAGFPDRATFWPTLMELDENGADIIEIGVPFSDPVADGPVVEEASRRALSDGVNLRG
ILAELAQRKGLVKAGLVLMGYLNPFLQYGYEKLAQDAARGGVHGFIVPDLPHEESGPLHRALKKEGIALIPLVGPNTSAE
RMALYAEEGEGYVYVVSVMGITGERGDVAPQVADTMRRARSVFSLPLALGFGLREPGQLRELPPDARPDAAVFGSALLNH
LEEGHSAEEFMARWK

Sequences:

>Translated_255_residues
MNLLEEKIRAANAAGRPALIPFLTAGFPDRATFWPTLMELDENGADIIEIGVPFSDPVADGPVVEEASRRALSDGVNLRG
ILAELAQRKGLVKAGLVLMGYLNPFLQYGYEKLAQDAARGGVHGFIVPDLPHEESGPLHRALKKEGIALIPLVGPNTSAE
RMALYAEEGEGYVYVVSVMGITGERGDVAPQVADTMRRARSVFSLPLALGFGLREPGQLRELPPDARPDAAVFGSALLNH
LEEGHSAEEFMARWK
>Mature_255_residues
MNLLEEKIRAANAAGRPALIPFLTAGFPDRATFWPTLMELDENGADIIEIGVPFSDPVADGPVVEEASRRALSDGVNLRG
ILAELAQRKGLVKAGLVLMGYLNPFLQYGYEKLAQDAARGGVHGFIVPDLPHEESGPLHRALKKEGIALIPLVGPNTSAE
RMALYAEEGEGYVYVVSVMGITGERGDVAPQVADTMRRARSVFSLPLALGFGLREPGQLRELPPDARPDAAVFGSALLNH
LEEGHSAEEFMARWK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=206, Percent_Identity=31.5533980582524, Blast_Score=107, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321412, Length=238, Percent_Identity=39.4957983193277, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_DESDA (B8J160)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480165.1
- GeneID:   7285285
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1587
- HOGENOM:   HBG391690
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27506; Mature: 27506

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLEEKIRAANAAGRPALIPFLTAGFPDRATFWPTLMELDENGADIIEIGVPFSDPVAD
CCHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
GPVVEEASRRALSDGVNLRGILAELAQRKGLVKAGLVLMGYLNPFLQYGYEKLAQDAARG
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVHGFIVPDLPHEESGPLHRALKKEGIALIPLVGPNTSAERMALYAEEGEGYVYVVSVMG
CCCEEECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHEEEEEECCCCEEEEEEEEC
ITGERGDVAPQVADTMRRARSVFSLPLALGFGLREPGQLRELPPDARPDAAVFGSALLNH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHH
LEEGHSAEEFMARWK
HHCCCCHHHHHHHCC
>Mature Secondary Structure
MNLLEEKIRAANAAGRPALIPFLTAGFPDRATFWPTLMELDENGADIIEIGVPFSDPVAD
CCHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
GPVVEEASRRALSDGVNLRGILAELAQRKGLVKAGLVLMGYLNPFLQYGYEKLAQDAARG
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVHGFIVPDLPHEESGPLHRALKKEGIALIPLVGPNTSAERMALYAEEGEGYVYVVSVMG
CCCEEECCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHEEEEEECCCCEEEEEEEEC
ITGERGDVAPQVADTMRRARSVFSLPLALGFGLREPGQLRELPPDARPDAAVFGSALLNH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHH
LEEGHSAEEFMARWK
HHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA