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The map label for this gene is cofH [H]

Identifier: 220904549

GI number: 220904549

Start: 1508784

End: 1509929

Strand: Direct

Name: cofH [H]

Synonym: Ddes_1280

Alternate gene names: 220904549

Gene position: 1508784-1509929 (Clockwise)

Preceding gene: 220904548

Following gene: 220904550

Centisome position: 52.51

GC content: 57.5

Gene sequence:

>1146_bases
GTGAGCACCTTTTCCCCCGCACACAGCCCCTTTGTCATGCATGGCGGACAAGATAAGGATTTTGCCGATGTGCGCGAAGC
CGCAGCCCTGGCCGCAGCCGGTCAACGGCTTGACCGCAGCGCCGCAGAAATTCTGTATTACAAGGCCGACCTTCACACAC
TCGCGCAGCTTGCTCATGCCATGCGCTTGCGCCTGCATCCTGACCCCGTGGTTACATATGTAGGCGACCGCAACATCAAT
TATTCGAACGTATGCGTGTGCGCCTGCCGATTCTGCGCTTTTTTCCGCGCCCCGGATCACGAAGACTGTTACGTCATAAG
CCGGGATGAAATGAGCCGCAAAGTGGAAGAAACGTTGGCTCTCGGCGGTACGCAGATATTGCTCCAGGGCGGTCACCACC
CCGACCTGCCGCTGGAATGGTATGAAGATCTTTTACGCTGGCTGCGGCAGACGTGGCCGCAGCTGCATATTCATGCCTTT
TCACCACCGGAAATATTCTTCTGGGCTGAAAAATTCGATATTTCTGTTGCCGAAGTGCTGCGCCGCCTGAAAGAGGCCGG
TCTGCATTCCCTGCCGGGCGGCGGGGCTGAAATCCTGCATACTGGCATACGCGCCCAGGTTTCACCCAATAAATGCACGG
CTGAGCAATGGCTCGATGTTATGGAAGAAGCGCACAAGCTGGGGCTGCGCACCACCGCCACAATGATGTTCGGCCATGAG
GAAAAAGCCTCGCACAGATTGGACCATCTTTTTGCAGTGCGTGAGGTACAGGATCGCACCCACGGTTTTACCGCATTCAT
TCCCTGGACCTTCCAGCCTGCCCACACACGTATCGAGGTCCCCCCCCTGCCTGCCCCGGCCTACTTGCGCCTGCTGGCAG
TTTCGCGTCTTGTGCTGGACAACGTGCCCAACATCCAGTCCTCCTGGGTGACCATGGGGCCACAGGTGGCCCAGTTGGCT
CTTTTTTACGGGGCCAACGATTTCGGATCCCTGATGATCGAAGAAAACGTGGTGGCTGCGGCAGGCGTGTCCTATCATAT
GAGCCGGGCCGATATTCACAAGGTGATCCACGCTGCGGGCTTCACCCCCATACAGCGCACCATGGACTACAGGGCTGTAG
ACCCGCAACCGGATGTGAAACTGTAA

Upstream 100 bases:

>100_bases
CCGAACTGGAGCATGTCATCCGCGCCTCGGGCTTTACGCCTGTTCGCCGTAACGCCACGTTTAACACCCTCAATGATACC
GACAGTCCGGAGGCGCGCCA

Downstream 100 bases:

>100_bases
CAGGACGGACCATAGCGGAAAAAGGTGTGCCCGGGCGTTTTGCCCATTGCAGGCAAACCTTTCCGCGCCGGATTTTGCGG
CGTTTGCTCGCCAGGCAGAC

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSTFSPAHSPFVMHGGQDKDFADVREAAALAAAGQRLDRSAAEILYYKADLHTLAQLAHAMRLRLHPDPVVTYVGDRNIN
YSNVCVCACRFCAFFRAPDHEDCYVISRDEMSRKVEETLALGGTQILLQGGHHPDLPLEWYEDLLRWLRQTWPQLHIHAF
SPPEIFFWAEKFDISVAEVLRRLKEAGLHSLPGGGAEILHTGIRAQVSPNKCTAEQWLDVMEEAHKLGLRTTATMMFGHE
EKASHRLDHLFAVREVQDRTHGFTAFIPWTFQPAHTRIEVPPLPAPAYLRLLAVSRLVLDNVPNIQSSWVTMGPQVAQLA
LFYGANDFGSLMIEENVVAAAGVSYHMSRADIHKVIHAAGFTPIQRTMDYRAVDPQPDVKL

Sequences:

>Translated_381_residues
MSTFSPAHSPFVMHGGQDKDFADVREAAALAAAGQRLDRSAAEILYYKADLHTLAQLAHAMRLRLHPDPVVTYVGDRNIN
YSNVCVCACRFCAFFRAPDHEDCYVISRDEMSRKVEETLALGGTQILLQGGHHPDLPLEWYEDLLRWLRQTWPQLHIHAF
SPPEIFFWAEKFDISVAEVLRRLKEAGLHSLPGGGAEILHTGIRAQVSPNKCTAEQWLDVMEEAHKLGLRTTATMMFGHE
EKASHRLDHLFAVREVQDRTHGFTAFIPWTFQPAHTRIEVPPLPAPAYLRLLAVSRLVLDNVPNIQSSWVTMGPQVAQLA
LFYGANDFGSLMIEENVVAAAGVSYHMSRADIHKVIHAAGFTPIQRTMDYRAVDPQPDVKL
>Mature_380_residues
STFSPAHSPFVMHGGQDKDFADVREAAALAAAGQRLDRSAAEILYYKADLHTLAQLAHAMRLRLHPDPVVTYVGDRNINY
SNVCVCACRFCAFFRAPDHEDCYVISRDEMSRKVEETLALGGTQILLQGGHHPDLPLEWYEDLLRWLRQTWPQLHIHAFS
PPEIFFWAEKFDISVAEVLRRLKEAGLHSLPGGGAEILHTGIRAQVSPNKCTAEQWLDVMEEAHKLGLRTTATMMFGHEE
KASHRLDHLFAVREVQDRTHGFTAFIPWTFQPAHTRIEVPPLPAPAYLRLLAVSRLVLDNVPNIQSSWVTMGPQVAQLAL
FYGANDFGSLMIEENVVAAAGVSYHMSRADIHKVIHAAGFTPIQRTMDYRAVDPQPDVKL

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42800; Mature: 42669

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFSPAHSPFVMHGGQDKDFADVREAAALAAAGQRLDRSAAEILYYKADLHTLAQLAHA
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
MRLRLHPDPVVTYVGDRNINYSNVCVCACRFCAFFRAPDHEDCYVISRDEMSRKVEETLA
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
LGGTQILLQGGHHPDLPLEWYEDLLRWLRQTWPQLHIHAFSPPEIFFWAEKFDISVAEVL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHCCHHHHHH
RRLKEAGLHSLPGGGAEILHTGIRAQVSPNKCTAEQWLDVMEEAHKLGLRTTATMMFGHE
HHHHHCCCCCCCCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCH
EKASHRLDHLFAVREVQDRTHGFTAFIPWTFQPAHTRIEVPPLPAPAYLRLLAVSRLVLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEECEECCCCCEEECCCCCHHHHHHHHHHHHHHHH
NVPNIQSSWVTMGPQVAQLALFYGANDFGSLMIEENVVAAAGVSYHMSRADIHKVIHAAG
CCCCCCCCCEECCHHHHHHHHHHCCCCHHCEEEECCCHHHCCHHHHHHHHHHHHHHHHCC
FTPIQRTMDYRAVDPQPDVKL
CCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
STFSPAHSPFVMHGGQDKDFADVREAAALAAAGQRLDRSAAEILYYKADLHTLAQLAHA
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
MRLRLHPDPVVTYVGDRNINYSNVCVCACRFCAFFRAPDHEDCYVISRDEMSRKVEETLA
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
LGGTQILLQGGHHPDLPLEWYEDLLRWLRQTWPQLHIHAFSPPEIFFWAEKFDISVAEVL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHCCHHHHHH
RRLKEAGLHSLPGGGAEILHTGIRAQVSPNKCTAEQWLDVMEEAHKLGLRTTATMMFGHE
HHHHHCCCCCCCCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCH
EKASHRLDHLFAVREVQDRTHGFTAFIPWTFQPAHTRIEVPPLPAPAYLRLLAVSRLVLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEECEECCCCCEEECCCCCHHHHHHHHHHHHHHHH
NVPNIQSSWVTMGPQVAQLALFYGANDFGSLMIEENVVAAAGVSYHMSRADIHKVIHAAG
CCCCCCCCCEECCHHHHHHHHHHCCCCHHCEEEECCCHHHCCHHHHHHHHHHHHHHHHCC
FTPIQRTMDYRAVDPQPDVKL
CCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]