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The map label for this gene is cofH [H]

Identifier: 220904548

GI number: 220904548

Start: 1507645

End: 1508787

Strand: Direct

Name: cofH [H]

Synonym: Ddes_1279

Alternate gene names: 220904548

Gene position: 1507645-1508787 (Clockwise)

Preceding gene: 220904545

Following gene: 220904549

Centisome position: 52.47

GC content: 57.48

Gene sequence:

>1143_bases
ATGTTAGACGCTGCATACTACGCCGCACTGGGCCTTTCGTCCATCCATGAAAAAGTGCTTTCAGGGCAGCGGCTGAGCTT
TGAAGACGGCCTTGCCCTGTTCCATTGTCCCGACGTTACCGCCGTGGGAGCGCTGGCTCTGCATGTGCGCTGCCGTTTGC
ACGGACACAAGGCTTTTTACGTGGTAAACCGGCAGATCAACTATACCAATGTTTGTGTCAACGGCTGTACGTTTTGCGCT
TTCCGGCGAGATGAGGAAAACGAAGCAGGCGCGTTTGCCCTGAGCCTTGATGACGTGCTGGACCGTCTGCGCGACGCGGA
CTCTACCCCACTGCACCTTGACGAACTGCACATAGTGGGCGGCTGCCACCCCACGCGGCCTCTTGCGTGGTTTGAAGACA
TGATACGCACCGTGCGGCATTTTAACCCAAACTTGCCTGTAAAAGCTTTTACGGCTGTGGAGATTGAACATTTCTCACGG
CTTGAAGGCATCAGCACCCTTGAGGTTCTTCGACGGCTTCAGGCAGCCGGGCTGGTCATGATGCCAGGCGGCGGCGCAGA
AATATTTGATGAAGAACTGCGCCCGCAGATTTGTCCTCACAAGGCCGATGCAACCGCATGGCTGCGCATTTCTGGCGAAG
CACACAGCCTGGGCATCAAGACCAACTGCACCATGCTCTACGGCCACCTGGAAACCTACGAACACCGGATCGACCACCTT
TGTCGCCTGCGTGAGCAGCAGGATAAAAGCGGCGGCTTCACCTGCTTTATTCCGCTGCCCTTCCTCACAGAAAACAGCCG
GCTCAAGCTGCCGGAAGACAAAGTTGGCCCGCAGCGCGGCCTTGACCAGTTGCGCACGGTGGCAGTCTCCCGCCTTATGC
TGGACAATATTCCCCACCTTAAAGCCTACTGGATAATGATGGGCCACAAACTGGCCCCCCTGGCCCTGTGGTACGGCGCC
GATGATTTGGATGGAACCATCATCGAAGAACGCATCGGCCATATGGCTGGCGCCCAGTCGGCGCAAGGCATGACCATTGC
CGAACTGGAGCATGTCATCCGCGCCTCGGGCTTTACGCCTGTTCGCCGTAACGCCACGTTTAACACCCTCAATGATACCG
ACAGTCCGGAGGCGCGCCAGTGA

Upstream 100 bases:

>100_bases
GAAAAGCTGAAGACATATATTCCTCACATTGACAGCTTGCGGCGGGCGGCCTACTCTGCTTGCGGCCCTTCCGGCCCAAT
CAACCCCACAGGATTTCATC

Downstream 100 bases:

>100_bases
GCACCTTTTCCCCCGCACACAGCCCCTTTGTCATGCATGGCGGACAAGATAAGGATTTTGCCGATGTGCGCGAAGCCGCA
GCCCTGGCCGCAGCCGGTCA

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MLDAAYYAALGLSSIHEKVLSGQRLSFEDGLALFHCPDVTAVGALALHVRCRLHGHKAFYVVNRQINYTNVCVNGCTFCA
FRRDEENEAGAFALSLDDVLDRLRDADSTPLHLDELHIVGGCHPTRPLAWFEDMIRTVRHFNPNLPVKAFTAVEIEHFSR
LEGISTLEVLRRLQAAGLVMMPGGGAEIFDEELRPQICPHKADATAWLRISGEAHSLGIKTNCTMLYGHLETYEHRIDHL
CRLREQQDKSGGFTCFIPLPFLTENSRLKLPEDKVGPQRGLDQLRTVAVSRLMLDNIPHLKAYWIMMGHKLAPLALWYGA
DDLDGTIIEERIGHMAGAQSAQGMTIAELEHVIRASGFTPVRRNATFNTLNDTDSPEARQ

Sequences:

>Translated_380_residues
MLDAAYYAALGLSSIHEKVLSGQRLSFEDGLALFHCPDVTAVGALALHVRCRLHGHKAFYVVNRQINYTNVCVNGCTFCA
FRRDEENEAGAFALSLDDVLDRLRDADSTPLHLDELHIVGGCHPTRPLAWFEDMIRTVRHFNPNLPVKAFTAVEIEHFSR
LEGISTLEVLRRLQAAGLVMMPGGGAEIFDEELRPQICPHKADATAWLRISGEAHSLGIKTNCTMLYGHLETYEHRIDHL
CRLREQQDKSGGFTCFIPLPFLTENSRLKLPEDKVGPQRGLDQLRTVAVSRLMLDNIPHLKAYWIMMGHKLAPLALWYGA
DDLDGTIIEERIGHMAGAQSAQGMTIAELEHVIRASGFTPVRRNATFNTLNDTDSPEARQ
>Mature_380_residues
MLDAAYYAALGLSSIHEKVLSGQRLSFEDGLALFHCPDVTAVGALALHVRCRLHGHKAFYVVNRQINYTNVCVNGCTFCA
FRRDEENEAGAFALSLDDVLDRLRDADSTPLHLDELHIVGGCHPTRPLAWFEDMIRTVRHFNPNLPVKAFTAVEIEHFSR
LEGISTLEVLRRLQAAGLVMMPGGGAEIFDEELRPQICPHKADATAWLRISGEAHSLGIKTNCTMLYGHLETYEHRIDHL
CRLREQQDKSGGFTCFIPLPFLTENSRLKLPEDKVGPQRGLDQLRTVAVSRLMLDNIPHLKAYWIMMGHKLAPLALWYGA
DDLDGTIIEERIGHMAGAQSAQGMTIAELEHVIRASGFTPVRRNATFNTLNDTDSPEARQ

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42457; Mature: 42457

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAAYYAALGLSSIHEKVLSGQRLSFEDGLALFHCPDVTAVGALALHVRCRLHGHKAFY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHEEEEEEEECCEEEE
VVNRQINYTNVCVNGCTFCAFRRDEENEAGAFALSLDDVLDRLRDADSTPLHLDELHIVG
EEECCCCEEEEEECCEEEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEEEEEE
GCHPTRPLAWFEDMIRTVRHFNPNLPVKAFTAVEIEHFSRLEGISTLEVLRRLQAAGLVM
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHCCCHHHHHHHHHHHCCEEE
MPGGGAEIFDEELRPQICPHKADATAWLRISGEAHSLGIKTNCTMLYGHLETYEHRIDHL
ECCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEEHHHHHHHHHHHHH
CRLREQQDKSGGFTCFIPLPFLTENSRLKLPEDKVGPQRGLDQLRTVAVSRLMLDNIPHL
HHHHHHCCCCCCEEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
KAYWIMMGHKLAPLALWYGADDLDGTIIEERIGHMAGAQSAQGMTIAELEHVIRASGFTP
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
VRRNATFNTLNDTDSPEARQ
CCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MLDAAYYAALGLSSIHEKVLSGQRLSFEDGLALFHCPDVTAVGALALHVRCRLHGHKAFY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHEEEEEEEECCEEEE
VVNRQINYTNVCVNGCTFCAFRRDEENEAGAFALSLDDVLDRLRDADSTPLHLDELHIVG
EEECCCCEEEEEECCEEEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCEEEEEEEEEE
GCHPTRPLAWFEDMIRTVRHFNPNLPVKAFTAVEIEHFSRLEGISTLEVLRRLQAAGLVM
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHCCCHHHHHHHHHHHCCEEE
MPGGGAEIFDEELRPQICPHKADATAWLRISGEAHSLGIKTNCTMLYGHLETYEHRIDHL
ECCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEEHHHHHHHHHHHHH
CRLREQQDKSGGFTCFIPLPFLTENSRLKLPEDKVGPQRGLDQLRTVAVSRLMLDNIPHL
HHHHHHCCCCCCEEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
KAYWIMMGHKLAPLALWYGADDLDGTIIEERIGHMAGAQSAQGMTIAELEHVIRASGFTP
HHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
VRRNATFNTLNDTDSPEARQ
CCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]