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The map label for this gene is mdtK [H]

Identifier: 220904546

GI number: 220904546

Start: 1504713

End: 1506155

Strand: Reverse

Name: mdtK [H]

Synonym: Ddes_1277

Alternate gene names: 220904546

Gene position: 1506155-1504713 (Counterclockwise)

Preceding gene: 220904547

Following gene: 220904540

Centisome position: 52.42

GC content: 53.92

Gene sequence:

>1443_bases
ATGTTTCAGAAATATTTTTCTTTTGCAGAAGCCCGCCGTTTTTACAGTATCGGCCTGCCGATTTTTATTGCGCAGCTTTC
ACAGACCGGCATGACATTTGTTGATACCGCCATGGCCGGGCAGTATAGCGCCGAGCACATGGCCGCTGTGGCCGTTGGCA
GCAGCGTATGGGTTCCCATATCCTTGTTGGGTATCGGTTGCCTGCTGGCCTTGCCGCCCCTGAGCGCACAGGTTGTCGGG
GCGGGCAGACCTGAAAAGGCGGCCCATTTATTGCGGCAGGGCTTGTGGCTGACCTTGGGGCTGAGCATCGTTCTGATGAG
CCTTTTTTATACAATTTCTTGGAATATGGAATCATTTGGCCTGAATCCGGAAATGGCGCACATTTCCGGAGGCTTTTTGC
GGGCCATCATGTGGGGGCTGCCGGGCTTCATGCTGTTTGTGAACTTGCGCAGTTTTCTTGAGGGCTTTGCCCGCACCCGG
CCAGCCATGATCATCGGTATGCTTGGCCTGACCGTAAACATACCTTGTAATTTTATCTTTATTTACGGCAAATTCGGCAT
GCCCGAACTGGGCGGCGTTGGTTGTGGGGTAGCTACGGCCATAAGTTTTTGGTCTATGGCACTGGGTATGTTTTTTTATT
TGCACCGGGACCCGCAATGTGCCCAGGTGCGCCCGTTATTTATGCCACTTTGGCGTAACTGCAGAAAAACGGGTGAAAAA
CCTGCCCGGGAAGCACAGGAAAACTGCCTGCCGCGTTTTGATGCACCCCTGATCTGGCGGATTTTACGCATCGGCATGCC
TGGCGCTCTGGCCTTGTTCTTTGAGGTTTCACTTTTCGCTCTCAGCGCCATCCTGCTGGCCCCCTTGGGGACCATTATGG
TGGCTGGACACCAGATCGCCCTCAACTTCGGTTCACTGGCCTTTATGGTTCCGCTTTCTCTTTGCATGACCGTGACGATC
CGCGTGGGCCGCTGCCTGGGCGCACAGGACCTGGAACGAGCCAGAATGGCCGCGCGTACGGCCCTTGTACTCAGCGTTGT
TTTTGCCCTGATAATTGCTGCCATCACCATATCGTTCCGGCAGAATATCGTGCGCATTTATACGGACAATCCGGAAGTAA
CTGCTTTGGCCATGCACCTGTTGCTTTTTCAGGCGGCCTATCAGGTGGTGGATGGCCTTCAGGTCACAGGTATAGGAATT
TTGCGGGGCTATAACGATACACGTGTTATTTCCGTCATCTGTTTTATTGCCTACTGGGTCATTGGCCTGCCCCTGGGCTT
CACGCTGGCACGCACCAATATTATTGTGCCGTATCTGGGAGCTGAGGGTTTCTGGATTGCCTATATTGTAGCTTTGGGCT
TCGGCGCTCTGTGCTACCTGTCGCGAGTGCGCTTTCTGCACAGTCTTGCACCGCAGGTGGTGTTCAGCCGCATAAGGCGA
TGA

Upstream 100 bases:

>100_bases
GATTGCGGCAGTACCGTTTCCAGGCCGGGCTTTTTGTCTGCCGGATTTGCGGCGTCTTGCCGCTGCGCTTCGCGAACCAC
CTACCACCCCCATACCTCAC

Downstream 100 bases:

>100_bases
GGCAGGCCGCCTGCGCGACAATCCTTCGACAGGCGCGTCGGGCTGGCATGGAAAGGTCACTGTTTGCGGCGGCGGGCCAT
TTTCCAAGTATATTTCATGA

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MFQKYFSFAEARRFYSIGLPIFIAQLSQTGMTFVDTAMAGQYSAEHMAAVAVGSSVWVPISLLGIGCLLALPPLSAQVVG
AGRPEKAAHLLRQGLWLTLGLSIVLMSLFYTISWNMESFGLNPEMAHISGGFLRAIMWGLPGFMLFVNLRSFLEGFARTR
PAMIIGMLGLTVNIPCNFIFIYGKFGMPELGGVGCGVATAISFWSMALGMFFYLHRDPQCAQVRPLFMPLWRNCRKTGEK
PAREAQENCLPRFDAPLIWRILRIGMPGALALFFEVSLFALSAILLAPLGTIMVAGHQIALNFGSLAFMVPLSLCMTVTI
RVGRCLGAQDLERARMAARTALVLSVVFALIIAAITISFRQNIVRIYTDNPEVTALAMHLLLFQAAYQVVDGLQVTGIGI
LRGYNDTRVISVICFIAYWVIGLPLGFTLARTNIIVPYLGAEGFWIAYIVALGFGALCYLSRVRFLHSLAPQVVFSRIRR

Sequences:

>Translated_480_residues
MFQKYFSFAEARRFYSIGLPIFIAQLSQTGMTFVDTAMAGQYSAEHMAAVAVGSSVWVPISLLGIGCLLALPPLSAQVVG
AGRPEKAAHLLRQGLWLTLGLSIVLMSLFYTISWNMESFGLNPEMAHISGGFLRAIMWGLPGFMLFVNLRSFLEGFARTR
PAMIIGMLGLTVNIPCNFIFIYGKFGMPELGGVGCGVATAISFWSMALGMFFYLHRDPQCAQVRPLFMPLWRNCRKTGEK
PAREAQENCLPRFDAPLIWRILRIGMPGALALFFEVSLFALSAILLAPLGTIMVAGHQIALNFGSLAFMVPLSLCMTVTI
RVGRCLGAQDLERARMAARTALVLSVVFALIIAAITISFRQNIVRIYTDNPEVTALAMHLLLFQAAYQVVDGLQVTGIGI
LRGYNDTRVISVICFIAYWVIGLPLGFTLARTNIIVPYLGAEGFWIAYIVALGFGALCYLSRVRFLHSLAPQVVFSRIRR
>Mature_480_residues
MFQKYFSFAEARRFYSIGLPIFIAQLSQTGMTFVDTAMAGQYSAEHMAAVAVGSSVWVPISLLGIGCLLALPPLSAQVVG
AGRPEKAAHLLRQGLWLTLGLSIVLMSLFYTISWNMESFGLNPEMAHISGGFLRAIMWGLPGFMLFVNLRSFLEGFARTR
PAMIIGMLGLTVNIPCNFIFIYGKFGMPELGGVGCGVATAISFWSMALGMFFYLHRDPQCAQVRPLFMPLWRNCRKTGEK
PAREAQENCLPRFDAPLIWRILRIGMPGALALFFEVSLFALSAILLAPLGTIMVAGHQIALNFGSLAFMVPLSLCMTVTI
RVGRCLGAQDLERARMAARTALVLSVVFALIIAAITISFRQNIVRIYTDNPEVTALAMHLLLFQAAYQVVDGLQVTGIGI
LRGYNDTRVISVICFIAYWVIGLPLGFTLARTNIIVPYLGAEGFWIAYIVALGFGALCYLSRVRFLHSLAPQVVFSRIRR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=452, Percent_Identity=22.787610619469, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI153792564, Length=421, Percent_Identity=21.1401425178147, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI38261960, Length=457, Percent_Identity=19.6936542669584, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI48994890, Length=479, Percent_Identity=47.3903966597077, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI226510954, Length=435, Percent_Identity=22.9885057471264, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321821, Length=391, Percent_Identity=22.5063938618926, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320545, Length=429, Percent_Identity=21.4452214452214, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 52811; Mature: 52811

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQKYFSFAEARRFYSIGLPIFIAQLSQTGMTFVDTAMAGQYSAEHMAAVAVGSSVWVPI
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
SLLGIGCLLALPPLSAQVVGAGRPEKAAHLLRQGLWLTLGLSIVLMSLFYTISWNMESFG
HHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
LNPEMAHISGGFLRAIMWGLPGFMLFVNLRSFLEGFARTRPAMIIGMLGLTVNIPCNFIF
CCCCHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCEEEEE
IYGKFGMPELGGVGCGVATAISFWSMALGMFFYLHRDPQCAQVRPLFMPLWRNCRKTGEK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHCCCC
PAREAQENCLPRFDAPLIWRILRIGMPGALALFFEVSLFALSAILLAPLGTIMVAGHQIA
HHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LNFGSLAFMVPLSLCMTVTIRVGRCLGAQDLERARMAARTALVLSVVFALIIAAITISFR
EEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNIVRIYTDNPEVTALAMHLLLFQAAYQVVDGLQVTGIGILRGYNDTRVISVICFIAYWV
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECEEEEECCCCHHHHHHHHHHHHHH
IGLPLGFTLARTNIIVPYLGAEGFWIAYIVALGFGALCYLSRVRFLHSLAPQVVFSRIRR
HHHCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFQKYFSFAEARRFYSIGLPIFIAQLSQTGMTFVDTAMAGQYSAEHMAAVAVGSSVWVPI
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
SLLGIGCLLALPPLSAQVVGAGRPEKAAHLLRQGLWLTLGLSIVLMSLFYTISWNMESFG
HHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
LNPEMAHISGGFLRAIMWGLPGFMLFVNLRSFLEGFARTRPAMIIGMLGLTVNIPCNFIF
CCCCHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCEEEEE
IYGKFGMPELGGVGCGVATAISFWSMALGMFFYLHRDPQCAQVRPLFMPLWRNCRKTGEK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHCCCC
PAREAQENCLPRFDAPLIWRILRIGMPGALALFFEVSLFALSAILLAPLGTIMVAGHQIA
HHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LNFGSLAFMVPLSLCMTVTIRVGRCLGAQDLERARMAARTALVLSVVFALIIAAITISFR
EEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNIVRIYTDNPEVTALAMHLLLFQAAYQVVDGLQVTGIGILRGYNDTRVISVICFIAYWV
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECEEEEECCCCHHHHHHHHHHHHHH
IGLPLGFTLARTNIIVPYLGAEGFWIAYIVALGFGALCYLSRVRFLHSLAPQVVFSRIRR
HHHCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA