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The map label for this gene is rsmE [H]

Identifier: 220904439

GI number: 220904439

Start: 1377044

End: 1377802

Strand: Reverse

Name: rsmE [H]

Synonym: Ddes_1169

Alternate gene names: 220904439

Gene position: 1377802-1377044 (Counterclockwise)

Preceding gene: 220904440

Following gene: 220904438

Centisome position: 47.95

GC content: 59.29

Gene sequence:

>759_bases
ATGAGTTTAGCTTTTTTTTACCTTGCTCCTGAAAAGTGGGCAAAGGAAGTTTGTCTTGAAGACCAGGAAGCCCGCCACCT
GAGTCAGGTTTTACGGCTTGGCCCCGGAAATGAGGTGGGCCTGCTGGATGGTCGGGGCCGCAAAGGCCGCTTTATTGTAC
GCAAGGCAGGCAAAAAAAACGTCCAGCTTGAATGCATCAGCGAAGAGCGTCTGCCACTGCCCGAAGCCCGTGCGATAATA
GCCCTTGCGTTCAGCAAGGCTGTACGCCGGGGATTTTTCATGGAAAAGGCCGTAGAGTTAGGCGCTCACGGCATCTGGCT
TTGGCAGGGCGACCACAGCCAGGGCAAACTTTCAGCCGCAGCCGAAGAGGCGTGCCATGGTCAGCTTGTGGCCGGGGCCA
AACAGTGCGGCAACCCCTGGCTGCCCGAAATACGTGGTCTCGCCAATGGGGTTGACGAACTGGCAACTCTTGCCGCCGGG
GCCGATCACCGGATTTTGCCGTGGGAGGCACAGGATAATGTACCCATGCTTACCCCCGCCCTGGCAGGCCAGCCGGGTCT
GAGCGTGTATGTCATCGGGCCTGAAGGCGGCTTTTCATCACGTGAGCTGGATATGCTCAAGCGGTCCGGGTTTGTTGCGG
TAAGTCTGGGCGCCCGTGTGCTGCGTTGCGAGACAGCGGCCACCCTGTGCCTGGGGCTGCACTGGTGGGCCTCGCAACTG
CCGGAAAAGAGCTGTCCAGAAATGGAGCCTGGGGCATGA

Upstream 100 bases:

>100_bases
CCGGACTGGAAATTCGTAAGAACTACGGACGCCTCGTCTCCCTTCTGGATGGCTTCAAGGCCGTGCGGAAGGCAAGAACA
GGCAAGGAAAACATTGTAGC

Downstream 100 bases:

>100_bases
GTGTAAGCAAGCCCCTGCCTGAGCGCATGCGCCCGGATGATCTGGCCCTTTTTCTTGGGCAGACGCATCTCGGCAGCCGC
CTTCGTTCCCTCATGCAGGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSLAFFYLAPEKWAKEVCLEDQEARHLSQVLRLGPGNEVGLLDGRGRKGRFIVRKAGKKNVQLECISEERLPLPEARAII
ALAFSKAVRRGFFMEKAVELGAHGIWLWQGDHSQGKLSAAAEEACHGQLVAGAKQCGNPWLPEIRGLANGVDELATLAAG
ADHRILPWEAQDNVPMLTPALAGQPGLSVYVIGPEGGFSSRELDMLKRSGFVAVSLGARVLRCETAATLCLGLHWWASQL
PEKSCPEMEPGA

Sequences:

>Translated_252_residues
MSLAFFYLAPEKWAKEVCLEDQEARHLSQVLRLGPGNEVGLLDGRGRKGRFIVRKAGKKNVQLECISEERLPLPEARAII
ALAFSKAVRRGFFMEKAVELGAHGIWLWQGDHSQGKLSAAAEEACHGQLVAGAKQCGNPWLPEIRGLANGVDELATLAAG
ADHRILPWEAQDNVPMLTPALAGQPGLSVYVIGPEGGFSSRELDMLKRSGFVAVSLGARVLRCETAATLCLGLHWWASQL
PEKSCPEMEPGA
>Mature_251_residues
SLAFFYLAPEKWAKEVCLEDQEARHLSQVLRLGPGNEVGLLDGRGRKGRFIVRKAGKKNVQLECISEERLPLPEARAIIA
LAFSKAVRRGFFMEKAVELGAHGIWLWQGDHSQGKLSAAAEEACHGQLVAGAKQCGNPWLPEIRGLANGVDELATLAAGA
DHRILPWEAQDNVPMLTPALAGQPGLSVYVIGPEGGFSSRELDMLKRSGFVAVSLGARVLRCETAATLCLGLHWWASQLP
EKSCPEMEPGA

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=234, Percent_Identity=26.0683760683761, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27286; Mature: 27155

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAFFYLAPEKWAKEVCLEDQEARHLSQVLRLGPGNEVGLLDGRGRKGRFIVRKAGKKN
CCEEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCC
VQLECISEERLPLPEARAIIALAFSKAVRRGFFMEKAVELGAHGIWLWQGDHSQGKLSAA
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
AEEACHGQLVAGAKQCGNPWLPEIRGLANGVDELATLAAGADHRILPWEAQDNVPMLTPA
HHHHHCCHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCEECHH
LAGQPGLSVYVIGPEGGFSSRELDMLKRSGFVAVSLGARVLRCETAATLCLGLHWWASQL
HCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHC
PEKSCPEMEPGA
CCCCCCCCCCCC
>Mature Secondary Structure 
SLAFFYLAPEKWAKEVCLEDQEARHLSQVLRLGPGNEVGLLDGRGRKGRFIVRKAGKKN
CEEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCC
VQLECISEERLPLPEARAIIALAFSKAVRRGFFMEKAVELGAHGIWLWQGDHSQGKLSAA
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
AEEACHGQLVAGAKQCGNPWLPEIRGLANGVDELATLAAGADHRILPWEAQDNVPMLTPA
HHHHHCCHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCEECHH
LAGQPGLSVYVIGPEGGFSSRELDMLKRSGFVAVSLGARVLRCETAATLCLGLHWWASQL
HCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHC
PEKSCPEMEPGA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9729120 [H]